Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P15145 (AMPN_PIG)

Last modified October 13, 2009. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Aminopeptidase N
      Short name=AP-N
      Short name=pAPN
    EC=3.4.11.2
Alternative name(s):
    Alanyl aminopeptidase
    Microsomal aminopeptidase
    Aminopeptidase M
      Short name=AP-M
    gp130
    CD_antigen=CD13
Gene names
Name: ANPEP
OrganismSus scrofa (Pig)
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Hide | Top

Protein attributes

Sequence length963 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May be involved in the metabolism of regulatory peptides of diverse cell types and in the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis By similarity. It is able to degrade Leu-enkephalin and Met-enkephalin but not cholecystokinin CCK8, neuromedin C (GRP-10), somatostatin-14, substance P and vasoactive intestinal peptide. In case of porcine transmissible gastroenteritis coronavirus (TGEV) and porcine respiratory coronavirus (PRCoV) infections, serves as a receptor for TGEV and PRCoV spike glycoprotein in a species-specific manner.

Catalytic activity

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Homodimer. Interacts with TGEV and PRCoV spike glycoprotein. Ref.10

Subcellular location

Membrane; Single-pass type II membrane protein.

Post-translational modification

Sulfated.

Sequence similarities

Belongs to the peptidase M1 family.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 963962Aminopeptidase N
PRO_0000095083

Regions

Topological domain2 – 87Cytoplasmic
Transmembrane9 – 3224Signal-anchor for type II membrane protein Potential
Region33 – 6432Cytosolic Ser/Thr-rich junction
Region65 – 963899Metalloprotease
Region717 – 81397Interaction with TGEV spike glycoprotein

Sites

Active site3841 By similarity
Active site4721Proton donor Potential
Metal binding3831Zinc; catalytic By similarity
Metal binding3871Zinc; catalytic By similarity
Metal binding4061Zinc; catalytic By similarity

Amino acid modifications

Modified residue1711Sulfotyrosine Potential
Glycosylation1241N-linked (GlcNAc...) Potential
Glycosylation2291N-linked (GlcNAc...) Potential
Glycosylation2371N-linked (GlcNAc...) Potential
Glycosylation2581N-linked (GlcNAc...) Potential
Glycosylation2861N-linked (GlcNAc...) Potential
Glycosylation3141N-linked (GlcNAc...) Potential
Glycosylation3281N-linked (GlcNAc...) Potential
Glycosylation5061N-linked (GlcNAc...) Potential
Glycosylation5561N-linked (GlcNAc...) Potential
Glycosylation5691N-linked (GlcNAc...) Potential
Glycosylation6221N-linked (GlcNAc...) Potential
Glycosylation6461N-linked (GlcNAc...) Potential
Glycosylation7361N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict821F → N in CAA23291. Ref.4
Sequence conflict107 – 1082LL → FI in CAA23291. Ref.4
Sequence conflict1111E → G in CAA23291. Ref.4
Sequence conflict1261T → N in CAA23291. Ref.4
Sequence conflict1401S → F in CAA23291. Ref.4

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P15145-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 45751498F84CE899

FASTA963108,865
        10         20         30         40         50         60 
MAKGFYISKA LGILGILLGV AAVATIIALS VVYAQEKNKN AEHVPQAPTS PTITTTAAIT 

        70         80         90        100        110        120 
LDQSKPWNRY RLPTTLLPDS YFVTLRPYLT PNADGLYIFK GKSIVRLLCQ EPTDVIIIHS 

       130        140        150        160        170        180 
KKLNYTTQGH MVVLRGVGDS QVPEIDRTEL VELTEYLVVH LKGSLQPGHM YEMESEFQGE 

       190        200        210        220        230        240 
LADDLAGFYR SEYMEGNVKK VLATTQMQST DARKSFPCFD EPAMKATFNI TLIHPNNLTA 

       250        260        270        280        290        300 
LSNMPPKGSS TPLAEDPNWS VTEFETTPVM STYLLAYIVS EFQSVNETAQ NGVLIRIWAR 

       310        320        330        340        350        360 
PNAIAEGHGM YALNVTGPIL NFFANHYNTS YPLPKSDQIA LPDFNAGAME NWGLVTYREN 

       370        380        390        400        410        420 
ALLFDPQSSS ISNKERVVTV IAHELAHQWF GNLVTLAWWN DLWLNEGFAS YVEYLGADHA 

       430        440        450        460        470        480 
EPTWNLKDLI VPGDVYRVMA VDALASSHPL TTPAEEVNTP AQISEMFDSI SYSKGASVIR 

       490        500        510        520        530        540 
MLSNFLTEDL FKEGLASYLH AFAYQNTTYL DLWEHLQKAV DAQTSIRLPD TVRAIMDRWT 

       550        560        570        580        590        600 
LQMGFPVITV DTKTGNISQK HFLLDSESNV TRSSAFDYLW IVPISSIKNG VMQDHYWLRD 

       610        620        630        640        650        660 
VSQAQNDLFK TASDDWVLLN VNVTGYFQVN YDEDNWRMIQ HQLQTNLSVI PVINRAQVIY 

       670        680        690        700        710        720 
DSFNLATAHM VPVTLALDNT LFLNGEKEYM PWQAALSSLS YFSLMFDRSE VYGPMKKYLR 

       730        740        750        760        770        780 
KQVEPLFQHF ETLTKNWTER PENLMDQYSE INAISTACSN GLPQCENLAK TLFDQWMSDP 

       790        800        810        820        830        840 
ENNPIHPNLR STIYCNAIAQ GGQDQWDFAW GQLQQAQLVN EADKLRSALA CSNEVWLLNR 

       850        860        870        880        890        900 
YLGYTLNPDL IRKQDATSTI NSIASNVIGQ PLAWDFVQSN WKKLFQDYGG GSFSFSNLIQ 

       910        920        930        940        950        960 
GVTRRFSSEF ELQQLEQFKK NNMDVGFGSG TRALEQALEK TKANIKWVKE NKEVVLNWFI 


EHS

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Determinants essential for the transmissible gastroenteritis virus-receptor interaction reside within a domain of aminopeptidase-N that is distinct from the enzymatic site."
Delmas B., Gelfi J., Kut E., Sjoestroem H., Noren O., Laude H.
J. Virol. 68:5216-5224(1994) [PubMed: 7913510] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION OF RECEPTOR FUNCTIONAL DOMAINS FOR TGEV INFECTION.
[2]"Cloning of the pig aminopeptidase N gene. Identification of possible regulatory elements and the exon distribution in relation to the membrane-spanning region."
Olsen J., Sjoestroem H., Noren O.
FEBS Lett. 251:275-281(1989) [PubMed: 2568950] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 1-294.
[3]"Identification and characterization of the major stilbene-disulphonate- and concanavalin A-binding protein of the porcine renal brush-border membrane as aminopeptidase N."
See H., Reithmeier R.A.F.
Biochem. J. 271:147-155(1990) [PubMed: 1977382] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-40.
[4]"Evaluation and characterization of a porcine small intestine cDNA library: analysis of 839 clones."
Winteroe A.K., Fredholm M., Davies W.
Mamm. Genome 7:509-517(1996) [PubMed: 8672129] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-144.
Tissue: Small intestine.
[5]"Aminopeptidase N is a major receptor for the entero-pathogenic coronavirus TGEV."
Delmas B., Gelfi J., L'Haridon R., Vogel L.K., Sjostrom H., Noren O., Laude H.
Nature 357:417-420(1992) [PubMed: 1350661] [Abstract]
Cited for: CHARACTERIZATION OF TGEV RECEPTOR FUNCTION.
[6]"Further characterization of aminopeptidase-N as a receptor for coronaviruses."
Delmas B., Gelfi J., Sjostrom H., Noren O., Laude H.
Adv. Exp. Med. Biol. 342:293-298(1993) [PubMed: 7911642] [Abstract]
Cited for: CHARACTERIZATION OF PRCOV RECEPTOR FUNCTION.
[7]"Purification and characterization of aminopeptidase M from muscle and mucosa of the pig intestine."
Terashima H., Bunnett N.W.
J. Gastroenterol. 30:696-704(1995) [PubMed: 8963385] [Abstract]
Cited for: CHARACTERIZATION.
[8]"Interspecies aminopeptidase-N chimeras reveal species-specific receptor recognition by canine coronavirus, feline infectious peritonitis virus, and transmissible gastroenteritis virus."
Benbacer L., Kut E., Besnardeau L., Laude H., Delmas B.
J. Virol. 71:734-737(1997) [PubMed: 8985407] [Abstract]
Cited for: IDENTIFICATION OF RECEPTOR FUNCTIONAL DOMAINS FOR TGEV INFECTION.
[9]"Characterization of determinants involved in the feline infectious peritonitis virus receptor function of feline aminopeptidase N."
Hegyi A., Kolb A.F.
J. Gen. Virol. 79:1387-1391(1998) [PubMed: 9634079] [Abstract]
Cited for: IDENTIFICATION OF RECEPTOR FUNCTIONAL DOMAINS FOR TGEV INFECTION.
[10]"Subunit structured of pig small-intestinal brush-border aminopeptidase N."
Benajiba A., Maroux S.
Biochem. J. 197:573-580(1981) [PubMed: 6119979] [Abstract]
Cited for: SUBUNIT.
[11]"Tyrosine sulfation, a post-translational modification of microvillar enzymes in the small intestinal enterocyte."
Danielsen E.M.
EMBO J. 6:2891-2896(1987) [PubMed: 3121301] [Abstract]
Cited for: SULFATION.

Hide | Top

Cross-references

Sequence databases

Z29522 mRNA. Translation: CAA82641.1.
X16088 Genomic DNA. Translation: CAA34216.1. Different termination.
F14846 mRNA. Translation: CAA23291.1.
PIRA53984.
RefSeqNP_999442.1.
UniGeneSsc.820

3D structure databases

ModBaseSearch...

Protein family/group databases

MEROPSM01.001.

Genome annotation databases

GeneID397520.
KEGGssc:397520.

Organism-specific databases

CTD397520.

Phylogenomic databases

HOVERGENP15145.

Enzyme and pathway databases

BRENDA3.4.11.2. 249.

Family and domain databases

InterProIPR006025. Pept_M_Zn_BS.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERPTHR11533. Peptidase_M1. 1 hit.
PfamPF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSPR00756. ALADIPTASE.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

PMAP-CutDBP15145.

Hide | Top

Entry information

Entry nameAMPN_PIG
AccessionPrimary (citable) accession number: P15145
Secondary accession number(s): Q29242
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: October 13, 2009
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Hide | Top

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents