P15141 (SPIKE_ADE07) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fiber protein
Short name=SPIKE

Alternative name(s):
Protein IV

Gene names

ORF Names:

L5

Organism

Human adenovirus B serotype 7 (HAdV-7) (Human adenovirus 7)

Taxonomic identifier

10519 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Adenoviridae › Mastadenovirus › Human adenovirus B › Human adenovirus B1

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Forms spikes that protrude from each vertex of the icosahedral capsid. Interacts with host receptor CD46 to provide virion initial attachment to target cell. Fiber proteins are shed during virus entry, when virus is still at the cell surface. Ref.2
Subunit structure	Homotrimer. Interacts with host receptor CD46. Interacts (via N-terminal tail region) with pentons By similarity. Ref.3
Subcellular location	Virion By similarity. Host nucleus By similarity. Note: Anchored to the pentons, protrudes from the virion surface By similarity.
Induction	Expressed in the late phase of the viral replicative cycle.
Domain	The tail region anchors the fiber to penton base capsomers, whereas the shaft, built from several repeated motifs, allows the knob to protrude from the virion By similarity.
Miscellaneous	All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell By similarity.
Sequence similarities	Belongs to the adenoviridae fiber family.
Sequence caution	The sequence AAA53254.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Host-virus interaction Viral attachment to host cell Virus entry into host cell
Cellular component	Host nucleus Virion
Developmental stage	Late protein
Molecular function	Capsid protein
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: InterPro viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 343

343

Fiber protein

PRO_0000221790

Secondary structure

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343

Helix Strand Turn

Details...

Helix

147 – 150

4

Beta strand

151 – 153

3

Beta strand

174 – 183

10

Beta strand

186 – 195

10

Helix

198 – 201

4

Helix

202 – 205

4

Beta strand

207 – 217

11

Turn

225 – 227

3

Beta strand

228 – 230

3

Helix

250 – 253

4

Turn

257 – 259

3

Helix

265 – 271

7

Beta strand

272 – 280

9

Beta strand

286 – 298

13

Beta strand

305 – 315

11

Turn

323 – 325

3

Beta strand

334 – 340

7

Sequences

	Sequence		Length	Mass (Da)
	P15141 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 8AC724C472607CD2	FASTA	343	37,287
	10 20 30 40 50 60 MSNFNSSPVP TIFMSFFQMT KRVRLSDSFN PVYPYEDEST SQHPFINPGF ISPNGFTQSP 70 80 90 100 110 120 DGVLTLKCLT PLTTTGGSLQ LKVGGGLTID DTDGFLKENI SAATPLVKTG HSIGLSLGPG 130 140 150 160 170 180 LGTNENKLCA KLGEGLTFNS NNICIDDNIN TLWTGVNPTT ANCQIMASSE SNDCKLILTL 190 200 210 220 230 240 VKTGGLVTAF VYVIGVSNDF NMLTTHKNIN FTAELFFDST GNLLTSLSSL KTPLNHKSGQ 250 260 270 280 290 300 NMATGALTNA KGFMPSTTAY PFNVNSREKE NYIYGTCYYT ASDHTAFPID ISVMLNQRAL 310 320 330 340 NNETSYCIRV TWSWNTGVAP EVQTSATTLV TSPFTFYYIR EDD

References

[1]	"Characterization of the early region 3 and fiber genes of Ad7." Hong J.S., Mullis K.G., Engler J.A. Virology 167:545-553(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Gomen.
[2]	"Adenovirus serotype 7 retention in a late endosomal compartment prior to cytosol escape is modulated by fiber protein." Miyazawa N., Crystal R.G., Leopold P.L. J. Virol. 75:1387-1400(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[3]	"Avidity binding of human adenovirus serotypes 3 and 7 to the membrane cofactor CD46 triggers infection." Trinh H.V., Lesage G., Chennamparampil V., Vollenweider B., Burckhardt C.J., Schauer S., Havenga M., Greber U.F., Hemmi S. J. Virol. 86:1623-1637(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HUMAN CD46.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M23696 Genomic DNA. Translation: AAA53254.1. Different initiation.

PIR

ERADF7. F31830.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3EXW	X-ray	1.75	A/B/C	135-343	[»]

ProteinModelPortal

P15141.

SMR

P15141. Positions 147-343.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.10.25.20. 1 hit.
2.60.90.10. 1 hit.

InterPro

IPR000931. Adeno_fibre.
IPR000978. Adeno_fibre_knob.
IPR000939. Adenobir_fibre_prot_rpt/shaft.
IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
[Graphical view]

Pfam

PF00541. Adeno_knob. 1 hit.
PF00608. Adeno_shaft. 2 hits.
[Graphical view]

PRINTS

PR00307. ADENOVSFIBRE.

SUPFAM

SSF49835. Viral_att. 1 hit.
SSF51225. Viral_att_shaft. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P15141.

Entry information

Entry name

SPIKE_ADE07

Accession

Primary (citable) accession number: P15141

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program