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Protein

Cytochrome P450 4B1

Gene

Cyp4b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei315 – 3151Heme (covalent; via 1 link)By similarity
Metal bindingi453 – 4531Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. drug binding Source: RGD
  3. fluorene oxygenase activity Source: RGD
  4. heme binding Source: InterPro
  5. iron ion binding Source: InterPro
  6. monooxygenase activity Source: RGD

GO - Biological processi

  1. biphenyl metabolic process Source: RGD
  2. cellular aromatic compound metabolic process Source: RGD
  3. exogenous drug catabolic process Source: RGD
  4. fluorene metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_234118. Fatty acids.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4B1 (EC:1.14.14.1)
Alternative name(s):
CYPIVB1
Cytochrome P450 L-2
Cytochrome P450 isozyme 5
Gene namesi
Name:Cyp4b1
Synonyms:Cyp4b-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 5

Organism-specific databases

RGDi2480. Cyp4b1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 511510Cytochrome P450 4B1PRO_0000051822Add
BLAST

Proteomic databases

PRIDEiP15129.

PTM databases

PhosphoSiteiP15129.

Expressioni

Inductioni

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Gene expression databases

ExpressionAtlasiP15129. baseline and differential.
GenevestigatoriP15129.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013321.

Structurei

3D structure databases

ProteinModelPortaliP15129.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiP15129.
KOiK07426.
OMAiHSFAFIP.
PhylomeDBiP15129.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15129-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVLNFLSPSL SRLGLWASVV ILMVIVLKLF SLLLRRQKLA RAMDSFPGPP
60 70 80 90 100
THWLFGHALE IQKLGSLDKV VSWAQQFPHA HPLWFGQFVG FLNIYEPDYA
110 120 130 140 150
KAVYSRGDPK AADVYDFFLQ WIGKGLLVLD GPKWFQHRKL LTPGFHYDVL
160 170 180 190 200
KPYVAIFAES TRMMLDKWEK KASENKSFDI FCDVGHMALD TLMKCTFGKG
210 220 230 240 250
DSGLGHRDNS YYLAVSDLTL LMQQRIDSFQ YHNDFIYWLT PHGRRFLRAC
260 270 280 290 300
KIAHDHTDEV IRQRKAALQD EKERKKIQQR RHLDFLDILL GVRDESGIKL
310 320 330 340 350
SDAELRAEVD TFMFEGHDTT TSGISWFLYC MALYPEHQQL CREEVRGILG
360 370 380 390 400
DQDSFQWDDL AKMTYLTMCM KECFRLYPPV PQVYRQLNKP VTFVDGRSLP
410 420 430 440 450
AGSLISLHIY ALHRNSTVWP DPEVFDPLRF SPENAAGRHP FAFMPFSAGP
460 470 480 490 500
RNCIGQQFAM NEMKVVTALC LLRFEFSLDP SKMPIKVPQL ILRSKNGIHL
510
YLKPLASRSG K
Length:511
Mass (Da):58,936
Last modified:January 23, 2007 - v3
Checksum:iCEAAFB5BE2ED9944
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29853 mRNA. Translation: AAA41778.1.
BC074012 mRNA. Translation: AAH74012.1.
PIRiB40164.
RefSeqiNP_058695.2. NM_016999.2.
UniGeneiRn.86651.

Genome annotation databases

EnsembliENSRNOT00000013321; ENSRNOP00000013321; ENSRNOG00000009741.
GeneIDi24307.
KEGGirno:24307.
UCSCiRGD:2480. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29853 mRNA. Translation: AAA41778.1.
BC074012 mRNA. Translation: AAH74012.1.
PIRiB40164.
RefSeqiNP_058695.2. NM_016999.2.
UniGeneiRn.86651.

3D structure databases

ProteinModelPortaliP15129.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013321.

PTM databases

PhosphoSiteiP15129.

Proteomic databases

PRIDEiP15129.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013321; ENSRNOP00000013321; ENSRNOG00000009741.
GeneIDi24307.
KEGGirno:24307.
UCSCiRGD:2480. rat.

Organism-specific databases

CTDi1580.
RGDi2480. Cyp4b1.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiP15129.
KOiK07426.
OMAiHSFAFIP.
PhylomeDBiP15129.

Enzyme and pathway databases

ReactomeiREACT_234118. Fatty acids.

Miscellaneous databases

NextBioi602935.
PROiP15129.

Gene expression databases

ExpressionAtlasiP15129. baseline and differential.
GenevestigatoriP15129.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structures of cytochrome P-450 isozyme 5 from rabbit and rat and regulation of species-dependent expression and induction in lung and liver: identification of cytochrome P-450 gene subfamily IVB."
    Gasser R., Philpot R.M.
    Mol. Pharmacol. 35:617-625(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  3. "Purification and characterization of rat pulmonary cytochrome P-450."
    Imaoka S., Funae Y.
    J. Biochem. 108:33-36(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-11.
    Tissue: Lung.

Entry informationi

Entry nameiCP4B1_RAT
AccessioniPrimary (citable) accession number: P15129
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.