Cytochrome P450 4B1 - Rattus norvegicus (Rat)

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P15129 (CP4B1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome P450 4B1
EC=1.14.14.1

Alternative name(s):
CYPIVB1
Cytochrome P450 L-2
Cytochrome P450 isozyme 5

Gene names

Name:	Cyp4b1
Synonyms:	Cyp4b-1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O₂ = ROH + oxidized flavoprotein + H₂O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	biphenyl metabolic process Inferred from mutant phenotype PubMed 9310344. Source: RGD exogenous drug catabolic process Inferred from direct assay PubMed 8248926. Source: RGD fluorene metabolic process Inferred from direct assay PubMed 8248926. Source: RGD
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell intracellular membrane-bounded organelle Inferred from direct assay PubMed 8248926 PubMed 9310344. Source: RGD
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC drug binding Inferred from direct assay PubMed 8248926. Source: RGD electron carrier activity Inferred from electronic annotation. Source: InterPro fluorene oxygenase activity Inferred from direct assay PubMed 8248926. Source: RGD heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from direct assay PubMed 8248926. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.3

Chain

2 – 511

510

Cytochrome P450 4B1

PRO_0000051822

Sites

Metal binding

453

Iron (heme axial ligand) By similarity

Binding site

315

Heme (covalent; via 1 link) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P15129 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: CEAAFB5BE2ED9944
Show »

FASTA

511

58,936

        10         20         30         40         50         60 
MVLNFLSPSL SRLGLWASVV ILMVIVLKLF SLLLRRQKLA RAMDSFPGPP THWLFGHALE 

        70         80         90        100        110        120 
IQKLGSLDKV VSWAQQFPHA HPLWFGQFVG FLNIYEPDYA KAVYSRGDPK AADVYDFFLQ 

       130        140        150        160        170        180 
WIGKGLLVLD GPKWFQHRKL LTPGFHYDVL KPYVAIFAES TRMMLDKWEK KASENKSFDI 

       190        200        210        220        230        240 
FCDVGHMALD TLMKCTFGKG DSGLGHRDNS YYLAVSDLTL LMQQRIDSFQ YHNDFIYWLT 

       250        260        270        280        290        300 
PHGRRFLRAC KIAHDHTDEV IRQRKAALQD EKERKKIQQR RHLDFLDILL GVRDESGIKL 

       310        320        330        340        350        360 
SDAELRAEVD TFMFEGHDTT TSGISWFLYC MALYPEHQQL CREEVRGILG DQDSFQWDDL 

       370        380        390        400        410        420 
AKMTYLTMCM KECFRLYPPV PQVYRQLNKP VTFVDGRSLP AGSLISLHIY ALHRNSTVWP 

       430        440        450        460        470        480 
DPEVFDPLRF SPENAAGRHP FAFMPFSAGP RNCIGQQFAM NEMKVVTALC LLRFEFSLDP 

       490        500        510 
SKMPIKVPQL ILRSKNGIHL YLKPLASRSG K

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structures of cytochrome P-450 isozyme 5 from rabbit and rat and regulation of species-dependent expression and induction in lung and liver: identification of cytochrome P-450 gene subfamily IVB." Gasser R., Philpot R.M. Mol. Pharmacol. 35:617-625(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[3]	"Purification and characterization of rat pulmonary cytochrome P-450." Imaoka S., Funae Y. J. Biochem. 108:33-36(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M29853 mRNA. Translation: AAA41778.1. BC074012 mRNA. Translation: AAH74012.1.
IPI	IPI00206129.
PIR	B40164.
RefSeq	NP_058695.2. NM_016999.2.
UniGene	Rn.86651.
3D structure databases
ProteinModelPortal	P15129.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000013321.
PTM databases
PhosphoSite	P15129.
Proteomic databases
PRIDE	P15129.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000013321; ENSRNOP00000013321; ENSRNOG00000009741.
GeneID	24307.
KEGG	rno:24307.
UCSC	RGD:2480. rat.
Organism-specific databases
CTD	1580.
RGD	2480. Cyp4b1.
Phylogenomic databases
eggNOG	COG2124.
GeneTree	ENSGT00660000095395.
HOGENOM	HOG000233833.
HOVERGEN	HBG000182.
InParanoid	P15129.
KO	K07426.
OMA	WEEMARE.
OrthoDB	EOG4ZS93G.
Gene expression databases
Genevestigator	P15129.
GermOnline	ENSRNOG00000009741. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	602935.

Entry information

Entry name

CP4B1_RAT

Accession

Primary (citable) accession number: P15129

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 105 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents