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P15124

- GLNA_METCA

UniProt

P15124 - GLNA_METCA

Protein

Glutamine synthetase

Gene

glnA

Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    Enzyme regulationi

    The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase
    Gene namesi
    Name:glnA
    Ordered Locus Names:MCA1677
    OrganismiMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
    Taxonomic identifieri243233 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus
    ProteomesiUP000006821: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469Glutamine synthetasePRO_0000153243Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei398 – 3981O-AMP-tyrosineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Oligomer of 12 subunits arranged in the form of two hexagons.

    Protein-protein interaction databases

    STRINGi243233.MCA1677.

    Structurei

    3D structure databases

    ProteinModelPortaliP15124.
    SMRiP15124. Positions 2-469.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000005157.
    KOiK01915.
    OMAiDMLLMPI.
    OrthoDBiEOG6B360N.

    Family and domain databases

    Gene3Di3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    IPR001637. Gln_synth_I_adenylation_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    TIGRFAMsiTIGR00653. GlnA. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00182. GLNA_ADENYLATION. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P15124-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTPKDVLQII KEKEVRYVDL RFADTRGKEQ HVTVPASTID EAAFEEGKMF    50
    DGSSIAGWKG INESDMILMP DASTAVMDPF FDDPTLILRC DIVEPATMQG 100
    YERDPRSIAK RAEAYMKSTG IADTALFGPE NEFFIFDDVR WGANMSGSFY 150
    KVDSEEAGWN SEKVYEDGNI GHRPGVKGGY FPVPPVDSFQ DLRSAMCNTL 200
    EDMGMVVEVH HHEVATAGQC EIGVRCNTLV KKADEVLLLK YAVQNVAHAY 250
    GKTATFMPKP LVGDNGNGMH VHQSLAKDGK NLFSGDLYGG LSETALHYIG 300
    GIIKHAKALN AFCNASTNSY KRLVPGFEAP VMLAYSARNR SASIRIPYVM 350
    NPKARRIEVR FPDSTANPYL AFAAMLMAGL DGIQNKIHPG DAMDKDLYDL 400
    PPEEEKAIPQ VCYSFDQALE ALDKDREFLT RGGVFTDDMI DAYLDLKGQE 450
    VTRLRMSTHP VEFDMYYSL 469
    Length:469
    Mass (Da):52,089
    Last modified:December 7, 2004 - v2
    Checksum:i652DFDF8405AFFFB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti264 – 2641D → A in AAA25312. (PubMed:1969923)Curated
    Sequence conflicti275 – 2751L → V in AAA25312. (PubMed:1969923)Curated
    Sequence conflicti302 – 3065IIKHA → YISMP in AAA25312. (PubMed:1969923)Curated
    Sequence conflicti345 – 3451R → G in AAA25312. (PubMed:1969923)Curated
    Sequence conflicti453 – 4608RLRMSTHP → ACDEHSS in AAA25312. (PubMed:1969923)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M28472 Genomic DNA. Translation: AAA25312.1.
    AE017282 Genomic DNA. Translation: AAU92104.1.
    PIRiA37176.
    RefSeqiWP_010960935.1. NC_002977.6.
    YP_114120.1. NC_002977.6.

    Genome annotation databases

    EnsemblBacteriaiAAU92104; AAU92104; MCA1677.
    GeneIDi3103018.
    KEGGimca:MCA1677.
    PATRICi22607194. VBIMetCap22254_1706.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M28472 Genomic DNA. Translation: AAA25312.1 .
    AE017282 Genomic DNA. Translation: AAU92104.1 .
    PIRi A37176.
    RefSeqi WP_010960935.1. NC_002977.6.
    YP_114120.1. NC_002977.6.

    3D structure databases

    ProteinModelPortali P15124.
    SMRi P15124. Positions 2-469.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243233.MCA1677.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAU92104 ; AAU92104 ; MCA1677 .
    GeneIDi 3103018.
    KEGGi mca:MCA1677.
    PATRICi 22607194. VBIMetCap22254_1706.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000005157.
    KOi K01915.
    OMAi DMLLMPI.
    OrthoDBi EOG6B360N.

    Family and domain databases

    Gene3Di 3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR004809. Gln_synth_I.
    IPR001637. Gln_synth_I_adenylation_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    TIGRFAMsi TIGR00653. GlnA. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00182. GLNA_ADENYLATION. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing and expression of the glutamine synthetase structural gene (glnA) from the obligate methanotroph Methylococcus capsulatus (Bath)."
      Cardy D.L.N., Murrell J.C.
      J. Gen. Microbiol. 136:343-352(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 33009 / NCIMB 11132 / Bath.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33009 / NCIMB 11132 / Bath.

    Entry informationi

    Entry nameiGLNA_METCA
    AccessioniPrimary (citable) accession number: P15124
    Secondary accession number(s): Q607S8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3