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P15124 (GLNA_METCA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase
Gene names
Name:glnA
Ordered Locus Names:MCA1677
OrganismMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath) [Complete proteome] [HAMAP]
Taxonomic identifier243233 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Enzyme regulation

The activity of this enzyme is controlled by adenylation under conditions of abundant glutamine. The fully adenylated enzyme complex is inactive By similarity.

Subunit structure

Oligomer of 12 subunits arranged in the form of two hexagons.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 469469Glutamine synthetase
PRO_0000153243

Amino acid modifications

Modified residue3981O-AMP-tyrosine By similarity

Experimental info

Sequence conflict2641D → A in AAA25312. Ref.1
Sequence conflict2751L → V in AAA25312. Ref.1
Sequence conflict302 – 3065IIKHA → YISMP in AAA25312. Ref.1
Sequence conflict3451R → G in AAA25312. Ref.1
Sequence conflict453 – 4608RLRMSTHP → ACDEHSS in AAA25312. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P15124 [UniParc].

Last modified December 7, 2004. Version 2.
Checksum: 652DFDF8405AFFFB

FASTA46952,089
        10         20         30         40         50         60 
MTPKDVLQII KEKEVRYVDL RFADTRGKEQ HVTVPASTID EAAFEEGKMF DGSSIAGWKG 

        70         80         90        100        110        120 
INESDMILMP DASTAVMDPF FDDPTLILRC DIVEPATMQG YERDPRSIAK RAEAYMKSTG 

       130        140        150        160        170        180 
IADTALFGPE NEFFIFDDVR WGANMSGSFY KVDSEEAGWN SEKVYEDGNI GHRPGVKGGY 

       190        200        210        220        230        240 
FPVPPVDSFQ DLRSAMCNTL EDMGMVVEVH HHEVATAGQC EIGVRCNTLV KKADEVLLLK 

       250        260        270        280        290        300 
YAVQNVAHAY GKTATFMPKP LVGDNGNGMH VHQSLAKDGK NLFSGDLYGG LSETALHYIG 

       310        320        330        340        350        360 
GIIKHAKALN AFCNASTNSY KRLVPGFEAP VMLAYSARNR SASIRIPYVM NPKARRIEVR 

       370        380        390        400        410        420 
FPDSTANPYL AFAAMLMAGL DGIQNKIHPG DAMDKDLYDL PPEEEKAIPQ VCYSFDQALE 

       430        440        450        460 
ALDKDREFLT RGGVFTDDMI DAYLDLKGQE VTRLRMSTHP VEFDMYYSL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing and expression of the glutamine synthetase structural gene (glnA) from the obligate methanotroph Methylococcus capsulatus (Bath)."
Cardy D.L.N., Murrell J.C.
J. Gen. Microbiol. 136:343-352(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 33009 / NCIMB 11132 / Bath.
[2]"Genomic insights into methanotrophy: the complete genome sequence of Methylococcus capsulatus (Bath)."
Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S., Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E., Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J. expand/collapse author list , Tettelin H., Ren Q., Read T.D., DeBoy R.T., Seshadri R., Salzberg S.L., Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H., Holt I.E., Eidhammer I., Jonasen I., Vanaken S., Utterback T.R., Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A.
PLoS Biol. 2:1616-1628(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33009 / NCIMB 11132 / Bath.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M28472 Genomic DNA. Translation: AAA25312.1.
AE017282 Genomic DNA. Translation: AAU92104.1.
PIRA37176.
RefSeqYP_114120.1. NC_002977.6.

3D structure databases

ProteinModelPortalP15124.
SMRP15124. Positions 2-469.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243233.MCA1677.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU92104; AAU92104; MCA1677.
GeneID3103018.
KEGGmca:MCA1677.
PATRIC22607194. VBIMetCap22254_1706.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0174.
HOGENOMHOG000005157.
KOK01915.
OMADMLLMPI.
OrthoDBEOG6B360N.

Family and domain databases

Gene3D3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
TIGRFAMsTIGR00653. GlnA. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA_METCA
AccessionPrimary (citable) accession number: P15124
Secondary accession number(s): Q607S8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: December 7, 2004
Last modified: May 14, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families