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Protein

Urokinase-type plasminogen activator

Gene

PLAU

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activityi

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei217Charge relay systemBy similarity1
Active sitei272Charge relay systemBy similarity1
Active sitei373Charge relay systemBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processPlasminogen activation

Protein family/group databases

MEROPSiS01.231

Names & Taxonomyi

Protein namesi
Recommended name:
Urokinase-type plasminogen activator (EC:3.4.21.73)
Short name:
U-plasminogen activator
Short name:
uPA
Cleaved into the following 2 chains:
Gene namesi
Name:PLAU
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000002833721 – 434Urokinase-type plasminogen activatorAdd BLAST414
ChainiPRO_000002833821 – 171Urokinase-type plasminogen activator chain ABy similarityAdd BLAST151
ChainiPRO_0000028339173 – 434Urokinase-type plasminogen activator chain BBy similarityAdd BLAST262

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi40 ↔ 48By similarity
Disulfide bondi42 ↔ 60By similarity
Disulfide bondi62 ↔ 71By similarity
Disulfide bondi79 ↔ 158By similarity
Disulfide bondi96 ↔ 139By similarity
Disulfide bondi128 ↔ 152By similarity
Disulfide bondi162 ↔ 296Interchain (between A and B chains)PROSITE-ProRule annotation
Disulfide bondi202 ↔ 218By similarity
Disulfide bondi210 ↔ 285By similarity
Glycosylationi228N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi310 ↔ 379By similarity
Disulfide bondi342 ↔ 358By similarity
Disulfide bondi369 ↔ 397By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP15120

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000008143

Structurei

3D structure databases

ProteinModelPortaliP15120
SMRiP15120
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 72EGF-likePROSITE-ProRule annotationAdd BLAST37
Domaini79 – 158KringlePROSITE-ProRule annotationAdd BLAST80
Domaini173 – 421Peptidase S1PROSITE-ProRule annotationAdd BLAST249

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni159 – 172Connecting peptideAdd BLAST14

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Kringle, Signal

Phylogenomic databases

eggNOGiENOG410IGFI Eukaryota
COG5640 LUCA
HOGENOMiHOG000237314
HOVERGENiHBG008633
InParanoidiP15120
KOiK01348
PhylomeDBiP15120

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
Gene3Di2.40.20.10, 1 hit
InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000001 Kringle
IPR013806 Kringle-like
IPR018056 Kringle_CS
IPR038178 Kringle_sf
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00051 Kringle, 1 hit
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00130 KR, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
SSF57440 SSF57440, 1 hit
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS01186 EGF_2, 1 hit
PS50026 EGF_3, 1 hit
PS00021 KRINGLE_1, 1 hit
PS50070 KRINGLE_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15120-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLIIFLTVT LCTLVTGLDS VYIRQYYKLS HKHRPQHREC QCLNGGTCIT
60 70 80 90 100
YRFFSQIKRC LCPEGYGGLH CEIDTNSICY SGNGEDYRGM AEDPGCLYWD
110 120 130 140 150
HPSVIRWGDY HADLKNALQL GLGKHNYCRN PNGRSRPWCY TKRRYSIQET
160 170 180 190 200
PCSTIEKCER TCGQRSFSKY FKIVGGSQAE VETQPWIAGI FQNIMGTDQF
210 220 230 240 250
LCGGSLIDPC WVLTAAHCFY NPTKKQPNKS VYKVFLGKSI LNTNDEHEQV
260 270 280 290 300
FMVDEIISHP DFTDHTGGND NDIALIRIRT ASGQCAVESN YVRTVCLPEK
310 320 330 340 350
NLNLYDNTWC EIAGYGKQNS YDIYYAQRLM SATVNLISQD DCKNKYYDST
360 370 380 390 400
RVTDNMVCAG DPLWETDACK GDSGGPMVCE HNGRMTLYGI VSWGDGCAKK
410 420 430
NKPGVYTRVT RYLNWIDSNM NAVFTKSRSF REPK
Length:434
Mass (Da):49,400
Last modified:April 1, 1990 - v1
Checksum:iBD881048DD666A55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05187 mRNA Translation: AAA49131.1
J05188 Genomic DNA Translation: AAA49130.1
PIRiA35005
RefSeqiNP_990774.2, NM_205443.2
UniGeneiGga.817

Genome annotation databases

GeneIDi396424
KEGGigga:396424

Similar proteinsi

Entry informationi

Entry nameiUROK_CHICK
AccessioniPrimary (citable) accession number: P15120
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: March 28, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health