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Reviewed, UniProtKB/Swiss-Prot P15120 (UROK_CHICK)

Last modified September 1, 2009. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Urokinase-type plasminogen activator
      Short name=U-plasminogen activator
      Short name=uPA
    EC=3.4.21.73
Cleaved into the following 2 chains:
    1- Recommended name:
            Urokinase-type plasminogen activator chain A
    2- Recommended name:
            Urokinase-type plasminogen activator chain B
Gene names
Name: PLAU
OrganismGallus gallus (Chicken)
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

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Protein attributes

Sequence length434 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.

Catalytic activity

Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 EGF-like domain.

Contains 1 kringle domain.

Contains 1 peptidase S1 domain.

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Ontologies

Keywords
   Biological processPlasminogen activation
   Cellular componentSecreted
   DomainEGF-like domain
Kringle
Signal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 434414Urokinase-type plasminogen activator
PRO_0000028337
Chain21 – 171151Urokinase-type plasminogen activator chain A By similarity
PRO_0000028338
Chain173 – 434262Urokinase-type plasminogen activator chain B By similarity
PRO_0000028339

Regions

Domain36 – 7237EGF-like
Domain79 – 15880Kringle
Domain173 – 421249Peptidase S1
Region159 – 17214Connecting peptide

Sites

Active site2171Charge relay system By similarity
Active site2721Charge relay system By similarity
Active site3731Charge relay system By similarity

Amino acid modifications

Glycosylation2281N-linked (GlcNAc...) Potential
Disulfide bond40 ↔ 48 By similarity
Disulfide bond42 ↔ 60 By similarity
Disulfide bond62 ↔ 71 By similarity
Disulfide bond79 ↔ 158 By similarity
Disulfide bond96 ↔ 139 By similarity
Disulfide bond128 ↔ 152 By similarity
Disulfide bond162 ↔ 296Interchain (between A and B chains) By similarity
Disulfide bond202 ↔ 218 By similarity
Disulfide bond210 ↔ 285 By similarity
Disulfide bond310 ↔ 379 By similarity
Disulfide bond342 ↔ 358 By similarity
Disulfide bond369 ↔ 397 By similarity

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Sequences

Sequence LengthMass (Da)Tools
P15120-1 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: BD881048DD666A55

FASTA43449,400
        10         20         30         40         50         60 
MKLIIFLTVT LCTLVTGLDS VYIRQYYKLS HKHRPQHREC QCLNGGTCIT YRFFSQIKRC 

        70         80         90        100        110        120 
LCPEGYGGLH CEIDTNSICY SGNGEDYRGM AEDPGCLYWD HPSVIRWGDY HADLKNALQL 

       130        140        150        160        170        180 
GLGKHNYCRN PNGRSRPWCY TKRRYSIQET PCSTIEKCER TCGQRSFSKY FKIVGGSQAE 

       190        200        210        220        230        240 
VETQPWIAGI FQNIMGTDQF LCGGSLIDPC WVLTAAHCFY NPTKKQPNKS VYKVFLGKSI 

       250        260        270        280        290        300 
LNTNDEHEQV FMVDEIISHP DFTDHTGGND NDIALIRIRT ASGQCAVESN YVRTVCLPEK 

       310        320        330        340        350        360 
NLNLYDNTWC EIAGYGKQNS YDIYYAQRLM SATVNLISQD DCKNKYYDST RVTDNMVCAG 

       370        380        390        400        410        420 
DPLWETDACK GDSGGPMVCE HNGRMTLYGI VSWGDGCAKK NKPGVYTRVT RYLNWIDSNM 

       430 
NAVFTKSRSF REPK

« Hide

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References

[1]"The chicken urokinase-type plasminogen activator gene."
Leslie N.D., Kessler C.A., Bell S.M., Degen J.L.
J. Biol. Chem. 265:1339-1344(1990) [PubMed: 2295632] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

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Cross-references

Sequence databases

J05187 mRNA. Translation: AAA49131.1.
J05188 Genomic DNA. Translation: AAA49130.1.
IPIIPI00584377.
PIRA35005.
RefSeqNP_990774.1.
UniGeneGga.817

3D structure databases

HSSPHSSP built from PDB template 1EZX based on UniProtKB P00760.
ModBaseSearch...

Protein-protein interaction databases

STRINGP15120.

Protein family/group databases

MEROPSS01.232.

Genome annotation databases

EnsemblENSGALT00000008157; ENSGALP00000008143; ENSGALG00000005086; Gallus gallus. [Genome view]
GeneID396424.
KEGGgga:396424.

Organism-specific databases

CTD396424.

Phylogenomic databases

HOGENOMP15120.
HOVERGENP15120.

Enzyme and pathway databases

BRENDA3.4.21.73. 4.

Family and domain databases

InterProIPR006210. EGF-like.
IPR013032. EGF-like_reg_CS.
IPR000742. EGF_3.
IPR000001. Kringle.
IPR018056. Kringle_CS.
IPR018059. Kringle_sub.
IPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
[Graphical view]
Gene3DG3DSA:2.40.20.10. Kringle. 1 hit.
PfamPF00051. Kringle. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
PR00018. KRINGLE.
ProDomPD000395. Kringle. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00181. EGF. 1 hit.
SM00130. KR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
PROSITEPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00021. KRINGLE_1. 1 hit.
PS50070. KRINGLE_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameUROK_CHICK
AccessionPrimary (citable) accession number: P15120
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 1, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents