ID   MCPT2_MOUSE             Reviewed;         244 AA.
AC   P15119;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   24-JAN-2024, entry version 157.
DE   RecName: Full=Mast cell protease 2;
DE            Short=mMCP-2;
DE            EC=3.4.21.-;
DE   Flags: Precursor;
GN   Name=Mcpt2;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=1688433; DOI=10.1016/s0021-9258(19)40247-0;
RA   Serafin W.E., Reynolds D.S., Rogelj S., Lane W.S., Conder G.A.,
RA   Johnson S.S., Austen K.F., Stevens R.L.;
RT   "Identification and molecular cloning of a novel mouse mucosal mast cell
RT   serine protease.";
RL   J. Biol. Chem. 265:423-429(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BALB/cJ; TISSUE=Hematopoietic;
RX   PubMed=8098710; DOI=10.1016/s0021-9258(18)82134-2;
RA   Gurish M.F., Nadeau J.H., Johnson K.R., McNeil H.P., Grattan K.M.,
RA   Austen K.F., Stevens R.L.;
RT   "A closely linked complex of mouse mast cell-specific chymase genes on
RT   chromosome 14.";
RL   J. Biol. Chem. 268:11372-11379(1993).
CC   -!- TISSUE SPECIFICITY: Mucosal mast cells.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. Granzyme subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU00274}.
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DR   EMBL; J05177; AAA39972.1; -; mRNA.
DR   EMBL; L08486; AAA74555.1; -; Genomic_DNA.
DR   CCDS; CCDS27139.1; -.
DR   PIR; A34910; A34910.
DR   PIR; A46721; A46721.
DR   RefSeq; NP_032597.1; NM_008571.1.
DR   AlphaFoldDB; P15119; -.
DR   SMR; P15119; -.
DR   STRING; 10090.ENSMUSP00000015576; -.
DR   MEROPS; S01.003; -.
DR   GlyCosmos; P15119; 1 site, No reported glycans.
DR   GlyGen; P15119; 1 site.
DR   iPTMnet; P15119; -.
DR   PhosphoSitePlus; P15119; -.
DR   PaxDb; 10090-ENSMUSP00000015576; -.
DR   ProteomicsDB; 292197; -.
DR   DNASU; 17225; -.
DR   Ensembl; ENSMUST00000015576.6; ENSMUSP00000015576.5; ENSMUSG00000022226.7.
DR   GeneID; 17225; -.
DR   KEGG; mmu:17225; -.
DR   UCSC; uc007ubk.1; mouse.
DR   AGR; MGI:96938; -.
DR   CTD; 17225; -.
DR   MGI; MGI:96938; Mcpt2.
DR   VEuPathDB; HostDB:ENSMUSG00000022226; -.
DR   eggNOG; KOG3627; Eukaryota.
DR   GeneTree; ENSGT01030000234551; -.
DR   HOGENOM; CLU_006842_1_0_1; -.
DR   InParanoid; P15119; -.
DR   OMA; NGSKERC; -.
DR   OrthoDB; 2540265at2759; -.
DR   PhylomeDB; P15119; -.
DR   TreeFam; TF333630; -.
DR   BioGRID-ORCS; 17225; 1 hit in 77 CRISPR screens.
DR   PRO; PR:P15119; -.
DR   Proteomes; UP000000589; Chromosome 14.
DR   RNAct; P15119; Protein.
DR   Bgee; ENSMUSG00000022226; Expressed in mucous cell of stomach and 31 other cell types or tissues.
DR   ExpressionAtlas; P15119; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd00190; Tryp_SPc; 1.
DR   Gene3D; 2.40.10.10; Trypsin-like serine proteases; 2.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR001314; Peptidase_S1A.
DR   InterPro; IPR001254; Trypsin_dom.
DR   InterPro; IPR018114; TRYPSIN_HIS.
DR   InterPro; IPR033116; TRYPSIN_SER.
DR   PANTHER; PTHR24271:SF23; CHYMASE 2, MAST CELL-RELATED; 1.
DR   PANTHER; PTHR24271; KALLIKREIN-RELATED; 1.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR00722; CHYMOTRYPSIN.
DR   SMART; SM00020; Tryp_SPc; 1.
DR   SUPFAM; SSF50494; Trypsin-like serine proteases; 1.
DR   PROSITE; PS50240; TRYPSIN_DOM; 1.
DR   PROSITE; PS00134; TRYPSIN_HIS; 1.
DR   PROSITE; PS00135; TRYPSIN_SER; 1.
DR   Genevisible; P15119; MM.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Hydrolase; Protease; Reference proteome;
KW   Serine protease; Signal; Zymogen.
FT   SIGNAL          1..18
FT   PROPEP          19..20
FT                   /note="Activation peptide"
FT                   /id="PRO_0000027451"
FT   CHAIN           21..244
FT                   /note="Mast cell protease 2"
FT                   /id="PRO_0000027452"
FT   DOMAIN          21..242
FT                   /note="Peptidase S1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   ACT_SITE        65
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        109
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        202
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        44
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        50..66
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        143..208
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        174..187
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   CONFLICT        67
FT                   /note="N -> R (in Ref. 1; AAA39972)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        108
FT                   /note="Y -> N (in Ref. 1; AAA39972)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        178
FT                   /note="S -> N (in Ref. 1; AAA39972)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        194
FT                   /note="S -> L (in Ref. 1; AAA39972)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        210
FT                   /note="S -> G (in Ref. 1; AAA39972)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   244 AA;  26732 MW;  132E248B6E2339CC CRC64;
     MQALLFLMAL LLPSGAGAEE IIGGVEAKPH SRPYMAYLKF TTKNGSKERC GGFLIAPQFV
     MTAAHCNGSE ISVILGAHNI NKNEPTQQII KTEKTFVHPK FQYLSGFYDI MLLKLQKKAE
     LNSDVDVISL PSSSDFIKPG KMCWTAGWGK TGKNNPLSVT LREVELRIMD QEACKDHSDY
     DYQLQVCAGS PTTSKSIGQG DSGGPLVCDS VAHGIASSYE AKAPAVFTRI SYYLPWIYKV
     LKSK
//