P15119 (MCPT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mast cell protease 2
Short name=mMCP-2
EC=3.4.21.-

Gene names

Name:

Mcpt2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Tissue specificity	Mucosal mast cells.
Sequence similarities	Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from direct assay PubMed 9360993. Source: MGI
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18
	Propeptide	19 – 20	2	Activation peptide		PRO_0000027451
	Chain	21 – 244	224	Mast cell protease 2		PRO_0000027452
Regions
	Domain	21 – 242	222	Peptidase S1
Sites
	Active site	65	1	Charge relay system By similarity
	Active site	109	1	Charge relay system By similarity
	Active site	202	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	44	1	N-linked (GlcNAc...) Potential
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	143 ↔ 208		By similarity
	Disulfide bond	174 ↔ 187		By similarity
Experimental info
	Sequence conflict	67	1	N → R in AAA39972. Ref.1
	Sequence conflict	108	1	Y → N in AAA39972. Ref.1
	Sequence conflict	178	1	S → N in AAA39972. Ref.1
	Sequence conflict	194	1	S → L in AAA39972. Ref.1
	Sequence conflict	210	1	S → G in AAA39972. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P15119 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 132E248B6E2339CC	FASTA	244	26,732
	10 20 30 40 50 60 MQALLFLMAL LLPSGAGAEE IIGGVEAKPH SRPYMAYLKF TTKNGSKERC GGFLIAPQFV 70 80 90 100 110 120 MTAAHCNGSE ISVILGAHNI NKNEPTQQII KTEKTFVHPK FQYLSGFYDI MLLKLQKKAE 130 140 150 160 170 180 LNSDVDVISL PSSSDFIKPG KMCWTAGWGK TGKNNPLSVT LREVELRIMD QEACKDHSDY 190 200 210 220 230 240 DYQLQVCAGS PTTSKSIGQG DSGGPLVCDS VAHGIASSYE AKAPAVFTRI SYYLPWIYKV LKSK

References

[1]	"Identification and molecular cloning of a novel mouse mucosal mast cell serine protease." Serafin W.E., Reynolds D.S., Rogelj S., Lane W.S., Conder G.A., Johnson S.S., Austen K.F., Stevens R.L. J. Biol. Chem. 265:423-429(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A closely linked complex of mouse mast cell-specific chymase genes on chromosome 14." Gurish M.F., Nadeau J.H., Johnson K.R., McNeil H.P., Grattan K.M., Austen K.F., Stevens R.L. J. Biol. Chem. 268:11372-11379(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. Tissue: Hematopoietic.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J05177 mRNA. Translation: AAA39972.1. L08486 Genomic DNA. Translation: AAA74555.1.
IPI	IPI00127248.
PIR	A34910. A46721.
RefSeq	NP_032597.1. NM_008571.1.
UniGene	Mm.4409.
3D structure databases
ProteinModelPortal	P15119.
SMR	P15119. Positions 21-241.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000015576.
Protein family/group databases
MEROPS	S01.003.
Proteomic databases
PRIDE	P15119.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000015576; ENSMUSP00000015576; ENSMUSG00000022226.
GeneID	17225.
KEGG	mmu:17225.
Organism-specific databases
CTD	17225.
MGI	MGI:96938. Mcpt2.
Phylogenomic databases
eggNOG	COG5640.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	P15119.
KO	K08662.
OMA	PWIRTIL.
Gene expression databases
ArrayExpress	P15119.
Bgee	P15119.
CleanEx	MM_MCPT2.
Genevestigator	P15119.
GermOnline	ENSMUSG00000022226. Mus musculus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	291640.
SOURCE	Search...

Entry information

Entry name

MCPT2_MOUSE

Accession

Primary (citable) accession number: P15119

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program