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Protein

Mast cell protease 2

Gene

Mcpt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay systemBy similarity
Active sitei109 – 1091Charge relay systemBy similarity
Active sitei202 – 2021Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: GO_Central

GO - Biological processi

  1. protein processing Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Mast cell protease 2 (EC:3.4.21.-)
Short name:
mMCP-2
Gene namesi
Name:Mcpt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:96938. Mcpt2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: GO_Central
  2. intracellular Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Add
BLAST
Propeptidei19 – 202Activation peptidePRO_0000027451
Chaini21 – 244224Mast cell protease 2PRO_0000027452Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
Disulfide bondi143 ↔ 208PROSITE-ProRule annotation
Disulfide bondi174 ↔ 187PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP15119.
PRIDEiP15119.

Expressioni

Tissue specificityi

Mucosal mast cells.

Gene expression databases

BgeeiP15119.
CleanExiMM_MCPT2.
ExpressionAtlasiP15119. baseline and differential.
GenevestigatoriP15119.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000015576.

Structurei

3D structure databases

ProteinModelPortaliP15119.
SMRiP15119. Positions 21-241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 242222Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP15119.
KOiK08662.
OMAiNGSKERC.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP15119.
TreeFamiTF333630.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15119-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQALLFLMAL LLPSGAGAEE IIGGVEAKPH SRPYMAYLKF TTKNGSKERC
60 70 80 90 100
GGFLIAPQFV MTAAHCNGSE ISVILGAHNI NKNEPTQQII KTEKTFVHPK
110 120 130 140 150
FQYLSGFYDI MLLKLQKKAE LNSDVDVISL PSSSDFIKPG KMCWTAGWGK
160 170 180 190 200
TGKNNPLSVT LREVELRIMD QEACKDHSDY DYQLQVCAGS PTTSKSIGQG
210 220 230 240
DSGGPLVCDS VAHGIASSYE AKAPAVFTRI SYYLPWIYKV LKSK
Length:244
Mass (Da):26,732
Last modified:October 1, 1996 - v2
Checksum:i132E248B6E2339CC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti67 – 671N → R in AAA39972 (PubMed:1688433).Curated
Sequence conflicti108 – 1081Y → N in AAA39972 (PubMed:1688433).Curated
Sequence conflicti178 – 1781S → N in AAA39972 (PubMed:1688433).Curated
Sequence conflicti194 – 1941S → L in AAA39972 (PubMed:1688433).Curated
Sequence conflicti210 – 2101S → G in AAA39972 (PubMed:1688433).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05177 mRNA. Translation: AAA39972.1.
L08486 Genomic DNA. Translation: AAA74555.1.
CCDSiCCDS27139.1.
PIRiA34910.
A46721.
RefSeqiNP_032597.1. NM_008571.1.
UniGeneiMm.4409.

Genome annotation databases

EnsembliENSMUST00000015576; ENSMUSP00000015576; ENSMUSG00000022226.
GeneIDi17225.
KEGGimmu:17225.
UCSCiuc007ubk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05177 mRNA. Translation: AAA39972.1.
L08486 Genomic DNA. Translation: AAA74555.1.
CCDSiCCDS27139.1.
PIRiA34910.
A46721.
RefSeqiNP_032597.1. NM_008571.1.
UniGeneiMm.4409.

3D structure databases

ProteinModelPortaliP15119.
SMRiP15119. Positions 21-241.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000015576.

Protein family/group databases

MEROPSiS01.003.

Proteomic databases

MaxQBiP15119.
PRIDEiP15119.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015576; ENSMUSP00000015576; ENSMUSG00000022226.
GeneIDi17225.
KEGGimmu:17225.
UCSCiuc007ubk.1. mouse.

Organism-specific databases

CTDi17225.
MGIiMGI:96938. Mcpt2.

Phylogenomic databases

eggNOGiCOG5640.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP15119.
KOiK08662.
OMAiNGSKERC.
OrthoDBiEOG7RRF7Z.
PhylomeDBiP15119.
TreeFamiTF333630.

Miscellaneous databases

NextBioi291640.
PROiP15119.
SOURCEiSearch...

Gene expression databases

BgeeiP15119.
CleanExiMM_MCPT2.
ExpressionAtlasiP15119. baseline and differential.
GenevestigatoriP15119.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification and molecular cloning of a novel mouse mucosal mast cell serine protease."
    Serafin W.E., Reynolds D.S., Rogelj S., Lane W.S., Conder G.A., Johnson S.S., Austen K.F., Stevens R.L.
    J. Biol. Chem. 265:423-429(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A closely linked complex of mouse mast cell-specific chymase genes on chromosome 14."
    Gurish M.F., Nadeau J.H., Johnson K.R., McNeil H.P., Grattan K.M., Austen K.F., Stevens R.L.
    J. Biol. Chem. 268:11372-11379(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/c.
    Tissue: Hematopoietic.

Entry informationi

Entry nameiMCPT2_MOUSE
AccessioniPrimary (citable) accession number: P15119
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1996
Last modified: March 4, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.