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Protein

NADP-specific glutamate dehydrogenase

Gene

gdhA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.By similarity

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92SubstrateBy similarity1
Binding sitei113SubstrateBy similarity1
Binding sitei116SubstrateBy similarity1
Active sitei128Proton donorPROSITE-ProRule annotation1
Binding sitei167Substrate; via carbonyl oxygenBy similarity1
Sitei168Important for catalysisBy similarity1
Binding sitei211NADPBy similarity1
Binding sitei242NADPBy similarity1
Binding sitei380SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

SABIO-RKP15111.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
Short name:
NADP-GDH
Gene namesi
Name:gdhA
Ordered Locus Names:STM1299
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001827761 – 447NADP-specific glutamate dehydrogenaseAdd BLAST447

Proteomic databases

PaxDbiP15111.
PRIDEiP15111.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

STRINGi99287.STM1299.

Structurei

3D structure databases

ProteinModelPortaliP15111.
SMRiP15111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105D82. Bacteria.
COG0334. LUCA.
HOGENOMiHOG000243799.
KOiK00262.
OMAiVPWVDDA.
PhylomeDBiP15111.

Family and domain databases

CDDicd05313. NAD_bind_2_Glu_DH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR033524. Glu/Leu/Phe/Val_DH_AS.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
IPR033922. NAD_bind_Glu_DH.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15111-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQTCSLESF LNHVQKRDPH QTEFAQAVRE VMTTLWPFLE QNPRYRHMSL
60 70 80 90 100
LERLVEPERV IQFRVVWLDD KNQVQVNRAW RVQFNSAIGP YKGGMRFHPS
110 120 130 140 150
VNLSILKFLG FEQTFKNALT TLPMGGGKGG SDFDPKGKSE GEVMRFCQAL
160 170 180 190 200
MTELYRHLGP DTDVPAGDIG VGGREVGFMA GMMRKLSNNS SCVFTGKGLS
210 220 230 240 250
FGGSLIRPEA TGYGLVYFTE AMLKRHGLGF EGMRVAVSGS GNVAQYAIEK
260 270 280 290 300
AMAFGARVVT ASDSSGTVVD ESGFTPEKLA RLCEIKASRD GRVADYAREF
310 320 330 340 350
GLTYLEGQQP WSVPVDIALP CATQNELDVD AARVLIANGV KAVAEGANMP
360 370 380 390 400
TTIEATDLFL EAGVLFAPGK AANAGGVATS GLEMAQNAAR LSWKAEKVDA
410 420 430 440
RLHHIMLDIH HACVEYGGDN KHTNYVQGAN IAGFVKVADA MLAQGVI
Length:447
Mass (Da):48,574
Last modified:January 23, 2002 - v2
Checksum:iCB94B38B5042E0A2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti299E → D in AAA27131 (PubMed:2656714).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24021 Genomic DNA. Translation: AAA27131.1.
AE006468 Genomic DNA. Translation: AAL20224.1.
PIRiA33504.
RefSeqiNP_460265.1. NC_003197.1.
WP_000372869.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20224; AAL20224; STM1299.
GeneIDi1252817.
KEGGistm:STM1299.
PATRICi32381075. VBISalEnt20916_1380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24021 Genomic DNA. Translation: AAA27131.1.
AE006468 Genomic DNA. Translation: AAL20224.1.
PIRiA33504.
RefSeqiNP_460265.1. NC_003197.1.
WP_000372869.1. NC_003197.1.

3D structure databases

ProteinModelPortaliP15111.
SMRiP15111.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM1299.

Proteomic databases

PaxDbiP15111.
PRIDEiP15111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20224; AAL20224; STM1299.
GeneIDi1252817.
KEGGistm:STM1299.
PATRICi32381075. VBISalEnt20916_1380.

Phylogenomic databases

eggNOGiENOG4105D82. Bacteria.
COG0334. LUCA.
HOGENOMiHOG000243799.
KOiK00262.
OMAiVPWVDDA.
PhylomeDBiP15111.

Enzyme and pathway databases

SABIO-RKP15111.

Family and domain databases

CDDicd05313. NAD_bind_2_Glu_DH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR033524. Glu/Leu/Phe/Val_DH_AS.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
IPR033922. NAD_bind_Glu_DH.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHE4_SALTY
AccessioniPrimary (citable) accession number: P15111
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2002
Last modified: November 30, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.