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P15111

- DHE4_SALTY

UniProt

P15111 - DHE4_SALTY

Protein

NADP-specific glutamate dehydrogenase

Gene

gdhA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (23 Jan 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.By similarity

    Catalytic activityi

    L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei92 – 921SubstrateBy similarity
    Binding sitei113 – 1131SubstrateBy similarity
    Binding sitei116 – 1161SubstrateBy similarity
    Active sitei128 – 1281Proton donorPROSITE-ProRule annotation
    Binding sitei167 – 1671Substrate; via carbonyl oxygenBy similarity
    Sitei168 – 1681Important for catalysisBy similarity
    Binding sitei211 – 2111NADPBy similarity
    Binding sitei242 – 2421NADPBy similarity
    Binding sitei380 – 3801SubstrateBy similarity

    GO - Molecular functioni

    1. glutamate dehydrogenase (NADP+) activity Source: UniProtKB

    GO - Biological processi

    1. glutamate biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-1310-MONOMER.
    SABIO-RKP15111.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
    Short name:
    NADP-GDH
    Gene namesi
    Name:gdhA
    Ordered Locus Names:STM1299
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 447447NADP-specific glutamate dehydrogenasePRO_0000182776Add
    BLAST

    Proteomic databases

    PaxDbiP15111.
    PRIDEiP15111.

    Interactioni

    Subunit structurei

    Homohexamer.By similarity

    Protein-protein interaction databases

    STRINGi99287.STM1299.

    Structurei

    3D structure databases

    ProteinModelPortaliP15111.
    SMRiP15111. Positions 6-447.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0334.
    HOGENOMiHOG000243799.
    KOiK00262.
    OMAiIMLDIHA.
    OrthoDBiEOG65XN4D.
    PhylomeDBiP15111.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P15111-1 [UniParc]FASTAAdd to Basket

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    MDQTCSLESF LNHVQKRDPH QTEFAQAVRE VMTTLWPFLE QNPRYRHMSL    50
    LERLVEPERV IQFRVVWLDD KNQVQVNRAW RVQFNSAIGP YKGGMRFHPS 100
    VNLSILKFLG FEQTFKNALT TLPMGGGKGG SDFDPKGKSE GEVMRFCQAL 150
    MTELYRHLGP DTDVPAGDIG VGGREVGFMA GMMRKLSNNS SCVFTGKGLS 200
    FGGSLIRPEA TGYGLVYFTE AMLKRHGLGF EGMRVAVSGS GNVAQYAIEK 250
    AMAFGARVVT ASDSSGTVVD ESGFTPEKLA RLCEIKASRD GRVADYAREF 300
    GLTYLEGQQP WSVPVDIALP CATQNELDVD AARVLIANGV KAVAEGANMP 350
    TTIEATDLFL EAGVLFAPGK AANAGGVATS GLEMAQNAAR LSWKAEKVDA 400
    RLHHIMLDIH HACVEYGGDN KHTNYVQGAN IAGFVKVADA MLAQGVI 447
    Length:447
    Mass (Da):48,574
    Last modified:January 23, 2002 - v2
    Checksum:iCB94B38B5042E0A2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti299 – 2991E → D in AAA27131. (PubMed:2656714)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24021 Genomic DNA. Translation: AAA27131.1.
    AE006468 Genomic DNA. Translation: AAL20224.1.
    PIRiA33504.
    RefSeqiNP_460265.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL20224; AAL20224; STM1299.
    GeneIDi1252817.
    KEGGistm:STM1299.
    PATRICi32381075. VBISalEnt20916_1380.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24021 Genomic DNA. Translation: AAA27131.1 .
    AE006468 Genomic DNA. Translation: AAL20224.1 .
    PIRi A33504.
    RefSeqi NP_460265.1. NC_003197.1.

    3D structure databases

    ProteinModelPortali P15111.
    SMRi P15111. Positions 6-447.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM1299.

    Proteomic databases

    PaxDbi P15111.
    PRIDEi P15111.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL20224 ; AAL20224 ; STM1299 .
    GeneIDi 1252817.
    KEGGi stm:STM1299.
    PATRICi 32381075. VBISalEnt20916_1380.

    Phylogenomic databases

    eggNOGi COG0334.
    HOGENOMi HOG000243799.
    KOi K00262.
    OMAi IMLDIHA.
    OrthoDBi EOG65XN4D.
    PhylomeDBi P15111.

    Enzyme and pathway databases

    BioCyci SENT99287:GCTI-1310-MONOMER.
    SABIO-RK P15111.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000185. Glu_DH. 1 hit.
    PRINTSi PR00082. GLFDHDRGNASE.
    SMARTi SM00839. ELFV_dehydrog. 1 hit.
    [Graphical view ]
    PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Affinity labeling of a glutamyl peptide in the coenzyme binding site of NADP+-specific glutamate dehydrogenase of Salmonella typhimurium by 2-[(4-bromo-2,3-dioxobutyl)thio]-1,N6-ethenoadenosine 2',5'-bisphosphate."
      Bansal A., Dayton M.A., Zalkin H., Colman R.F.
      J. Biol. Chem. 264:9827-9835(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.

    Entry informationi

    Entry nameiDHE4_SALTY
    AccessioniPrimary (citable) accession number: P15111
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: January 23, 2002
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3