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Protein

Protocatechuate 3,4-dioxygenase beta chain

Gene

pcaH

Organism
Burkholderia cepacia (Pseudomonas cepacia)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays an essential role in the utilization of numerous aromatic and hydroaromatic compounds via the beta-ketoadipate pathway.

Catalytic activityi

3,4-dihydroxybenzoate + O2 = 3-carboxy-cis,cis-muconate.

Cofactori

Fe3+Note: Binds Fe3+ ion per subunit.

Pathway:ibeta-ketoadipate pathway

This protein is involved in step 1 of the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate.
Proteins known to be involved in this subpathway in this organism are:
  1. Protocatechuate 3,4-dioxygenase beta chain (pcaH), Protocatechuate 3,4-dioxygenase alpha chain (pcaG)
This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-carboxy-cis,cis-muconate from 3,4-dihydroxybenzoate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi107 – 1071Iron; catalyticBy similarity
Metal bindingi146 – 1461Iron; catalyticBy similarity
Metal bindingi159 – 1591Iron; catalyticBy similarity
Metal bindingi161 – 1611Iron; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00157; UER00264.

Names & Taxonomyi

Protein namesi
Recommended name:
Protocatechuate 3,4-dioxygenase beta chain (EC:1.13.11.3)
Alternative name(s):
3,4-PCD
Gene namesi
Name:pcaH
OrganismiBurkholderia cepacia (Pseudomonas cepacia)
Taxonomic identifieri292 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 235235Protocatechuate 3,4-dioxygenase beta chainPRO_0000085099Add
BLAST

Interactioni

Subunit structurei

The enzyme is an oligomer of 12 copies of the alpha and beta chains.

Structurei

3D structure databases

ProteinModelPortaliP15110.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.130.10. 1 hit.
InterProiIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR024756. PCDO_beta_N.
IPR012785. Protocat_dOase_b.
[Graphical view]
PfamiPF00775. Dioxygenase_C. 1 hit.
PF12391. PCDO_beta_N. 1 hit.
[Graphical view]
SUPFAMiSSF49482. SSF49482. 1 hit.
TIGRFAMsiTIGR02422. protocat_beta. 1 hit.
PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15110-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSPTILTPR DWPSHPAYVH PDYRSSVKRG PTRPMIPLKE RLRDQYAPVY
60 70 80 90 100
GAEDLGPLDH DLTKNAVKNG EPLGERIVVT GRVLDEGGKP VRNTLVEVWQ
110 120 130 140 150
ANAAGRYVHK VDQHDAPLDP NFLGAGRCMT DAEGRYRFLT IKPGAYPWGN
160 170 180 190 200
HPNAWRPNHI HFSLFGDYFG SRLVTQMYFP GDPLLAYDPI FQGTPEAARD
210 220 230
RLISRFSLDT TEEGHALGYE FDIVLRGRDA TPMER
Length:235
Mass (Da):26,550
Last modified:April 1, 1990 - v1
Checksum:iE8D00C95C8854324
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30791 Genomic DNA. Translation: AAA25924.1.
PIRiB33487.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30791 Genomic DNA. Translation: AAA25924.1.
PIRiB33487.

3D structure databases

ProteinModelPortaliP15110.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00157; UER00264.

Family and domain databases

Gene3Di2.60.130.10. 1 hit.
InterProiIPR000627. Intradiol_dOase_C.
IPR015889. Intradiol_dOase_core.
IPR024756. PCDO_beta_N.
IPR012785. Protocat_dOase_b.
[Graphical view]
PfamiPF00775. Dioxygenase_C. 1 hit.
PF12391. PCDO_beta_N. 1 hit.
[Graphical view]
SUPFAMiSSF49482. SSF49482. 1 hit.
TIGRFAMsiTIGR02422. protocat_beta. 1 hit.
PROSITEiPS00083. INTRADIOL_DIOXYGENAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genetic organization and sequence of the Pseudomonas cepacia genes for the alpha and beta subunits of protocatechuate 3,4-dioxygenase."
    Zylstra G.J., Olsen R.H., Ballou D.P.
    J. Bacteriol. 171:5915-5921(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiPCXB_BURCE
AccessioniPrimary (citable) accession number: P15110
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 26, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.