Glutamine synthetase - Bos taurus (Bovine)

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P15103 (GLNA_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 100. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glutamine synthetase
Short name=GS
EC=6.3.1.2

Alternative name(s):
Glutamate decarboxylase
EC=4.1.1.15
Glutamate--ammonia ligase

Gene names

Name:

GLUL

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential for proliferation of fetal skin fibroblasts. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity.
Catalytic activity	ATP + L-glutamate + NH₃ = ADP + phosphate + L-glutamine. L-glutamate = 4-aminobutanoate + CO₂.
Cofactor	Biotin By similarity. Magnesium or manganese By similarity.
Subunit structure	Homooctamer and homotetramer. Interacts with PALMD By similarity.
Subcellular location	Cytoplasm By similarity. Mitochondrion By similarity.
Post-translational modification	Ubiquitinated by ZNRF1 By similarity.
Sequence similarities	Belongs to the glutamine synthetase family.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase Lyase
PTM	Acetylation Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell proliferation Inferred from electronic annotation. Source: Compara glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro response to glucose stimulus Inferred from electronic annotation. Source: Compara
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate decarboxylase activity Inferred from electronic annotation. Source: EC glutamate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 373

372

Glutamine synthetase

PRO_0000153136

Amino acid modifications

Modified residue

N-acetylalanine By similarity

Modified residue

104

Phosphotyrosine By similarity

Modified residue

180

Phosphotyrosine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P15103 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: BBCC6A953FD7DAD5
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FASTA

373

42,031

        10         20         30         40         50         60 
MATSASSHLN KGIKQVYMAL PQGDKVQAMY IWIDGTGEGL RCKTRTLDSE PKCIEELPEW 

        70         80         90        100        110        120 
NFDGSSTFQS EGSNSDMYLV PAAMFRDPFR KDPNKLVFCE VFKYNRKPAE TNLRHTCKRI 

       130        140        150        160        170        180 
MDMVSNQRPW FGMEQEYTLM GTDGHPFGWP SNGFPGPQGP YYCGVGADKA YGRDIVEAHY 

       190        200        210        220        230        240 
RACLYAGIKI GGTNAEVMPA QWEFQIGPCE GIDMGDHLWV ARFILHRVCE DFGVIATFDP 

       250        260        270        280        290        300 
KPIPGNWNGA GCHTNFSTKA MREENGLKYI EEAIEKLSKR HQYHIRAYDP KGGLDNARRL 

       310        320        330        340        350        360 
TGFHETSNIN DFSAGVANRG ASIRIPRTVG QEKKGYFEDR RPSANCDPFA VTEALIRTCL 

       370 
LNETGDEPFQ YKN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Hypothalamus.
[2]	"The bovine glutamine synthase gene (GLUL) maps to 10q33 and a pseudogene (GLULP) to 16q21." Masabanda J., Wigger G., Eggen A., Stranzinger G., Fries R. Mamm. Genome 8:794-795(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 202-267.
[3]	"The pattern of EAAC1 and GLT-1 glutamate transporter expression by skeletal and adipose tissues of fattening cattle differs from that of glutamine synthetase." Matthews J.C., Etienne N.M.P. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 258-352. Tissue: Kidney.
[4]	"Distribution of glutamine synthetase and carbamoyl-phosphate synthetase I in vertebrate liver." Smith D.D. Jr., Campbell J.W. Proc. Natl. Acad. Sci. U.S.A. 85:160-164(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 267-373. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC103099 mRNA. Translation: AAI03100.1. Y10347 Genomic DNA. Translation: CAA71373.1. Sequence problems. AY186585 mRNA. Translation: AAO27470.1. J03604 mRNA. Translation: AAA87357.1.
IPI	IPI00760457.
PIR	A34006.
RefSeq	NP_001035564.1. NM_001040474.2.
UniGene	Bt.106825.
3D structure databases
ProteinModelPortal	P15103.
SMR	P15103. Positions 3-372.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000041778.
Proteomic databases
PRIDE	P15103.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000038488; ENSBTAP00000038301; ENSBTAG00000013631.
GeneID	281199.
KEGG	bta:281199.
Organism-specific databases
CTD	2752.
Phylogenomic databases
eggNOG	COG0174.
GeneTree	ENSGT00390000010047.
HOGENOM	HOG000061500.
HOVERGEN	HBG005847.
InParanoid	P15103.
KO	K01915.
OMA	YGIDIEF.
OrthoDB	EOG444KKD.
Gene expression databases
ArrayExpress	P15103.
Family and domain databases
Gene3D	3.10.20.70. 1 hit. 3.30.590.10. 1 hit.
InterPro	IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat_dom. IPR008146. Gln_synth_cat_dom. IPR027303. Gln_synth_gly_rich_site. IPR027302. Gln_synth_N_conserv_site. [Graphical view]
Pfam	PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view]
SUPFAM	SSF54368. Gln_synt_beta. 1 hit.
PROSITE	PS00180. GLNA_1. 1 hit. PS00181. GLNA_ATP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20805255.

Entry information

Entry name

GLNA_BOVIN

Accession

Primary (citable) accession number: P15103
Secondary accession number(s): O02850, Q3ZBU6, Q866Q7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 100 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents