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P15103

- GLNA_BOVIN

UniProt

P15103 - GLNA_BOVIN

Protein

Glutamine synthetase

Gene

GLUL

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Essential for proliferation of fetal skin fibroblasts. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity.By similarity

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
    L-glutamate = 4-aminobutanoate + CO2.

    Cofactori

    Biotin.By similarity
    Magnesium or manganese.By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC
    3. glutamate decarboxylase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell proliferation Source: Ensembl
    2. glutamine biosynthetic process Source: InterPro
    3. response to glucose Source: Ensembl

    Keywords - Molecular functioni

    Ligase, Lyase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_217400. Amino acid synthesis and interconversion (transamination).
    REACT_219505. Astrocytic Glutamate-Glutamine Uptake And Metabolism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase (EC:6.3.1.2)
    Short name:
    GS
    Alternative name(s):
    Glutamate decarboxylase (EC:4.1.1.15)
    Glutamate--ammonia ligase
    Gene namesi
    Name:GLUL
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 16

    Subcellular locationi

    Cytoplasm By similarity. Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 373372Glutamine synthetasePRO_0000153136Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei104 – 1041PhosphotyrosineBy similarity

    Post-translational modificationi

    Ubiquitinated by ZNRF1.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiP15103.

    Interactioni

    Subunit structurei

    Homooctamer and homotetramer. Interacts with PALMD By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000041778.

    Structurei

    3D structure databases

    ProteinModelPortaliP15103.
    SMRiP15103. Positions 3-372.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    GeneTreeiENSGT00390000010047.
    HOGENOMiHOG000061500.
    HOVERGENiHBG005847.
    InParanoidiP15103.
    KOiK01915.
    OMAiRIPRNVG.
    OrthoDBiEOG7CZK5G.
    TreeFamiTF300491.

    Family and domain databases

    Gene3Di3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15103-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATSASSHLN KGIKQVYMAL PQGDKVQAMY IWIDGTGEGL RCKTRTLDSE    50
    PKCIEELPEW NFDGSSTFQS EGSNSDMYLV PAAMFRDPFR KDPNKLVFCE 100
    VFKYNRKPAE TNLRHTCKRI MDMVSNQRPW FGMEQEYTLM GTDGHPFGWP 150
    SNGFPGPQGP YYCGVGADKA YGRDIVEAHY RACLYAGIKI GGTNAEVMPA 200
    QWEFQIGPCE GIDMGDHLWV ARFILHRVCE DFGVIATFDP KPIPGNWNGA 250
    GCHTNFSTKA MREENGLKYI EEAIEKLSKR HQYHIRAYDP KGGLDNARRL 300
    TGFHETSNIN DFSAGVANRG ASIRIPRTVG QEKKGYFEDR RPSANCDPFA 350
    VTEALIRTCL LNETGDEPFQ YKN 373
    Length:373
    Mass (Da):42,031
    Last modified:January 23, 2007 - v4
    Checksum:iBBCC6A953FD7DAD5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC103099 mRNA. Translation: AAI03100.1.
    Y10347 Genomic DNA. Translation: CAA71373.1. Sequence problems.
    AY186585 mRNA. Translation: AAO27470.1.
    J03604 mRNA. Translation: AAA87357.1.
    PIRiA34006.
    RefSeqiNP_001035564.1. NM_001040474.2.
    XP_005217320.1. XM_005217263.1.
    XP_005217321.1. XM_005217264.1.
    UniGeneiBt.106825.

    Genome annotation databases

    EnsembliENSBTAT00000038488; ENSBTAP00000038301; ENSBTAG00000013631.
    GeneIDi281199.
    KEGGibta:281199.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC103099 mRNA. Translation: AAI03100.1 .
    Y10347 Genomic DNA. Translation: CAA71373.1 . Sequence problems.
    AY186585 mRNA. Translation: AAO27470.1 .
    J03604 mRNA. Translation: AAA87357.1 .
    PIRi A34006.
    RefSeqi NP_001035564.1. NM_001040474.2.
    XP_005217320.1. XM_005217263.1.
    XP_005217321.1. XM_005217264.1.
    UniGenei Bt.106825.

    3D structure databases

    ProteinModelPortali P15103.
    SMRi P15103. Positions 3-372.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000041778.

    Proteomic databases

    PRIDEi P15103.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000038488 ; ENSBTAP00000038301 ; ENSBTAG00000013631 .
    GeneIDi 281199.
    KEGGi bta:281199.

    Organism-specific databases

    CTDi 2752.

    Phylogenomic databases

    eggNOGi COG0174.
    GeneTreei ENSGT00390000010047.
    HOGENOMi HOG000061500.
    HOVERGENi HBG005847.
    InParanoidi P15103.
    KOi K01915.
    OMAi RIPRNVG.
    OrthoDBi EOG7CZK5G.
    TreeFami TF300491.

    Enzyme and pathway databases

    Reactomei REACT_217400. Amino acid synthesis and interconversion (transamination).
    REACT_219505. Astrocytic Glutamate-Glutamine Uptake And Metabolism.

    Miscellaneous databases

    NextBioi 20805255.

    Family and domain databases

    Gene3Di 3.10.20.70. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Hypothalamus.
    2. "The bovine glutamine synthase gene (GLUL) maps to 10q33 and a pseudogene (GLULP) to 16q21."
      Masabanda J., Wigger G., Eggen A., Stranzinger G., Fries R.
      Mamm. Genome 8:794-795(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 202-267.
    3. "The pattern of EAAC1 and GLT-1 glutamate transporter expression by skeletal and adipose tissues of fattening cattle differs from that of glutamine synthetase."
      Matthews J.C., Etienne N.M.P.
      Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 258-352.
      Tissue: Kidney.
    4. "Distribution of glutamine synthetase and carbamoyl-phosphate synthetase I in vertebrate liver."
      Smith D.D. Jr., Campbell J.W.
      Proc. Natl. Acad. Sci. U.S.A. 85:160-164(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 267-373.
      Tissue: Liver.

    Entry informationi

    Entry nameiGLNA_BOVIN
    AccessioniPrimary (citable) accession number: P15103
    Secondary accession number(s): O02850, Q3ZBU6, Q866Q7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 110 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3