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P15102

- GLNA4_PHAVU

UniProt

P15102 - GLNA4_PHAVU

Protein

Glutamine synthetase leaf isozyme, chloroplastic

Gene
N/A
Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. nitrogen fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Nitrogen fixation

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase leaf isozyme, chloroplastic (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase
    Isozyme delta
    OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
    Taxonomic identifieri3885 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5050ChloroplastBy similarityAdd
    BLAST
    Chaini51 – 429379Glutamine synthetase leaf isozyme, chloroplasticPRO_0000011183Add
    BLAST

    Proteomic databases

    PRIDEiP15102.
    ProMEXiP15102.

    Expressioni

    Tissue specificityi

    Expressed in leaves and stems. Low levels detected in roots and nodules.1 Publication

    Interactioni

    Subunit structurei

    Homooctamer.

    Structurei

    3D structure databases

    ProteinModelPortaliP15102.
    SMRiP15102. Positions 61-412.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P15102-1 [UniParc]FASTAAdd to Basket

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    MAQILAPSTQ WQMRFTKSSR HASPITSNTW SSLLMKQNKK TSSAKFRVLA    50
    VKSDGSTINR LEGLLNLDIT PFTDKIIAEY IWIGGTGIDV RSKSRTISKP 100
    VEHPSELPKW NYDGSSTGQA PGEDSEVILY PQAIFKDPFR GGNNILVICD 150
    AYTPAGEPIP TNKRHRAAEV FSNPRVIAEV PWFGIEQEYT LLQTNVNWPL 200
    GWPVGGYPGP QGPYYCSAGA DKSFGRDISD AHYKACLFAG INISGTNGEV 250
    MPGQWEYQVG PSVGIEAGDH IWASRYILER ITEQAGVVLS LDPKPIEGDW 300
    NGAGCHTNYS TKSMREDGGF EVIKKAILNL SLRHKEHISA YGEGNERRLT 350
    GKHETASINT FSWGVANRGC SIRVGRDTEK NGKGYLEDRR PASNMDPYVV 400
    TSLLAESTLL WEPTLEAEAL AAQKLALKV 429
    Length:429
    Mass (Da):47,246
    Last modified:April 1, 1990 - v1
    Checksum:i0CA55624B1118AF8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12738 mRNA. Translation: CAA31234.1.
    PIRiS04031. AJFBQD.
    RefSeqiXP_007147796.1. XM_007147734.1.
    XP_007147797.1. XM_007147735.1.

    Genome annotation databases

    GeneIDi18628920.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12738 mRNA. Translation: CAA31234.1 .
    PIRi S04031. AJFBQD.
    RefSeqi XP_007147796.1. XM_007147734.1.
    XP_007147797.1. XM_007147735.1.

    3D structure databases

    ProteinModelPortali P15102.
    SMRi P15102. Positions 61-412.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P15102.
    ProMEXi P15102.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 18628920.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The chloroplast-located glutamine synthetase of Phaseolus vulgaris L.: nucleotide sequence, expression in different organs and uptake into isolated chloroplasts."
      Lightfoot D.A., Green N.K., Cullimore J.V.
      Plant Mol. Biol. 11:191-202(1988)
      [AGRICOLA] [Europe PMC]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: cv. Tendergreen.
      Tissue: Leaf.

    Entry informationi

    Entry nameiGLNA4_PHAVU
    AccessioniPrimary (citable) accession number: P15102
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are at least four isozymes of this enzyme in P.vulgaris.
    Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3