P15095 (RDRP_NMV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: RNA replication protein Alternative name(s): 186 kDa protein ORF1 protein Including the following 2 domains: RNA-directed RNA polymerase EC=2.7.7.48 Helicase EC=3.6.4.13
Organism	Narcissus mosaic virus (NMV) [Complete proteome]
Taxonomic identifier	12180 [NCBI]
Taxonomic lineage	Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Tymovirales › Alphaflexiviridae › Potexvirus
Virus host	Narcissus pseudonarcissus (Daffodil) [TaxID: 39639]

Protein attributes

Sequence length	1643 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA replication. The central part of this protein possibly functions as an ATP-binding helicase Probable.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). ATP + H2O = ADP + phosphate.
Sequence similarities	Belongs to the potexvirus/carlavirus RNA replication protein family. Contains 1 (+)RNA virus helicase ATP-binding domain. Contains 1 (+)RNA virus helicase C-terminal domain. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	RNA processing Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1643	1643	RNA replication protein		PRO_0000222549
Regions
	Domain	828 – 996	169	(+)RNA virus helicase ATP-binding
	Domain	997 – 1132	136	(+)RNA virus helicase C-terminal
	Domain	1372 – 1479	108	RdRp catalytic
	Nucleotide binding	868 – 875	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P15095 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: ADAE6FCF0FF2EC43	FASTA	1,643	186,305
	10 20 30 40 50 60 MAKVRAALER IRDPSVQTAL SEAAYTHVRP VLKESLVNCP YALSNDEADC LESFGITVNP 70 80 90 100 110 120 YANQTHTHAA CKVIENRMLE IVGMHLPKHS CTMLFLKRSK LRYMRRAAIL KDVFLNKDVE 130 140 150 160 170 180 PKDLFRYDRD TIRSRLQDID TKIAYMSDTL HFMSRREIVQ LFEDSPKLET LLATVVLPVE 190 200 210 220 230 240 ALHKRTSLYP SLYSINYSAK GFEYIPGNHG GGSYFHPYTT LEWLKVRMIN AEDFHKLSTG 250 260 270 280 290 300 FILTFQLVES LGANHLFIVQ KAKLLTPQMR TFCRDSLVTL PQVFCPAAMN ANRPLSKTKA 310 320 330 340 350 360 MQMLLYCKSV KQVTERDIYA KIRQIIPTSE LELYDPDEIV HLANYFFFVS SLDSITCYED 370 380 390 400 410 420 LLSSDIWMRL TRPLRTAVRK FVELFKGKQD FDKLLIALKW QPFSYSLEPV DFTAYFVSRR 430 440 450 460 470 480 VRTLAKMEDI SWHQAAELAR RLESEPDLLS WEDLCSKPIA DPLASVSTPP LATLTSPAIS 490 500 510 520 530 540 ESRVTDTSSP KLTNISVESI LHPTSSNHAT IAESLNPKFC GWTRGDFHHM IVNQPSDRLK 550 560 570 580 590 600 GCRSWSYTTS PDVDLMLENL LLTPIPWESR LSDILLTLNT PANACCIQIL DCSAASAWVD 610 620 630 640 650 660 WSVQPIKTFP VAFVGMGETT LTFEDDSTLP LKEGEFVFFP PEWLARHKYQ IKTASNLHLC 670 680 690 700 710 720 ATFLVLDTTL LGETLANNCI EPEPLRPTAS RKSCQPTPSV DKSGADSPPE KQIVTPIVDA 730 740 750 760 770 780 AVLSCRPKLQ DVTKPTKVLM AAETSDSVAD SLPWASWVNL LQKHGFKGNQ QQIAQDGQLI 790 800 810 820 830 840 IPISDIRKLP HIPFPEEVPE TLRETLKNIK RFPVEITMQH KRAGSYDSDI KNNRTGKLLS 850 860 870 880 890 900 QMDNKWKAAF AYKLQQEDRK VCGTIIHGCG GSGKSFAIQE WMRSLKEDQS VVTVVTPTVL 910 920 930 940 950 960 LRNDWQTKLP ILPADVFKTF EKSVIQPCNP ILVFDDYTKL PPGLIESVVM HHQNVVFIIL 970 980 990 1000 1010 1020 TGDNRQSVYH ETNPEAYIAA LPEAVEIFSP YCEFYLNATH RNVKDLANKL GVYSEREGKL 1030 1040 1050 1060 1070 1080 KVNFASHHLK ASRIPMLVPS TMKRNAMFDM GHHSMTYAGC QGLTAPKIQI LLDNHTQFCS 1090 1100 1110 1120 1130 1140 ERVLYTCLSR AVDRIHFINT GPTTGDYWAK LESTPYLKAF IDTYRDEKTE VYNSQPASAE 1150 1160 1170 1180 1190 1200 PTEPEAPATH FPTAPKPLLE PLVEKLTDKE AREIFSPAFG HSNAIQTEDS VVQLFQHQQA 1210 1220 1230 1240 1250 1260 KDETLYWATI DTRLAISTPE ANLREFNMKR DIGDILFMNY AKLMCLPPEP VPFEERLWKI 1270 1280 1290 1300 1310 1320 SADEVRNTYI SKPIGNLVNA ASRQSPDFPK NKIALFLKSQ WVKKTEKLGT LKVKPGQTIA 1330 1340 1350 1360 1370 1380 SFMQETVMLY GTMARYLRKM RRRFQPDNIF INCETTPEDL DKFIKSQWDF SRPAHTNDFT 1390 1400 1410 1420 1430 1440 AFDQSQDGAM LQFEVIKAKF FNIPAEIIEG YIYIKLNAAI FLGTLGIMRL SGEGPTFDAN 1450 1460 1470 1480 1490 1500 TECSIAYNAT RFHITDDTAQ VYAGDDMALD RVSIEKDSFN RLEKQLKLTS KPMFPKQVKG 1510 1520 1530 1540 1550 1560 DYAEFCGWVM TPAGIIKHSL KMHASIQLQK KINNIKESAR SYALDLRYAY KLGDELQEHL 1570 1580 1590 1600 1610 1620 NEVEADYHQQ SVRDMHLLHQ QDVLNKGSAS PPHVFEKTAD ANTAGSSKTH KRNALKKKKQ 1630 1640 TRIAEILPSP DATGLSSLPF RFF

References

[1]	"Nucleotide sequence of narcissus mosaic virus RNA." Zuidema D., Linthorst H.J.M., Huisman M.J., Asjes C.J., Bol J.F. J. Gen. Virol. 70:267-276(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13747 Genomic RNA. Translation: BAA02891.1.
PIR	RRWGNV. JT0470.
RefSeq	NP_040778.1. NC_001441.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1494016.
Family and domain databases
InterPro	IPR027351. (+)RNA_virus_helicase_core_dom. IPR007094. RNA-dir_pol_PSvirus. IPR002588. Tymovirus_MeTrfase. IPR001788. Tymovirus_RNA-dep_RNA_pol. [Graphical view]
Pfam	PF00978. RdRP_2. 1 hit. PF01443. Viral_helicase1. 1 hit. PF01660. Vmethyltransf. 1 hit. [Graphical view]
PROSITE	PS51657. PSRV_HELICASE. 1 hit. PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_NMV

Accession

Primary (citable) accession number: P15095

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program