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Protein

Dihydrofolate reductase HdrA

Gene

hdrA

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.1 Publication

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation1 Publication

Enzyme regulationi

Activity unstable at KCl and NaCl concentrations lower than 2 M and activity increases with increasing concentrations of KCl or NaCl.1 Publication

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase HdrA (hdrA)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei8NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei29SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 20NADPBy similarity7
Nucleotide bindingi47 – 48NADPBy similarity2
Nucleotide bindingi67 – 68NADPBy similarity2
Nucleotide bindingi101 – 108NADPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

BRENDAi1.5.1.3 2561
UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase HdrA (EC:1.5.1.3)
Short name:
DHFR A
Short name:
hDHFR-1
Gene namesi
Name:hdrA
Synonyms:folA
Ordered Locus Names:HVO_1279
OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Taxonomic identifieri309800 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHaloferacalesHaloferacaceaeHaloferax
Proteomesi
  • UP000008243 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864331 – 162Dihydrofolate reductase HdrAAdd BLAST162

Interactioni

Protein-protein interaction databases

STRINGi309800.HVO_1279

Structurei

Secondary structure

1162
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 11Combined sources9
Beta strandi13 – 17Combined sources5
Helixi27 – 36Combined sources10
Beta strandi38 – 40Combined sources3
Beta strandi42 – 45Combined sources4
Helixi46 – 51Combined sources6
Turni52 – 54Combined sources3
Beta strandi58 – 64Combined sources7
Beta strandi73 – 75Combined sources3
Beta strandi78 – 81Combined sources4
Helixi82 – 91Combined sources10
Beta strandi97 – 101Combined sources5
Helixi103 – 109Combined sources7
Helixi110 – 112Combined sources3
Beta strandi114 – 124Combined sources11
Beta strandi128 – 130Combined sources3
Turni136 – 138Combined sources3
Beta strandi139 – 146Combined sources8
Beta strandi151 – 157Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VDRX-ray2.55A/B1-162[»]
2ITHNMR-A1-162[»]
2JYBNMR-A1-162[»]
ProteinModelPortaliP15093
SMRiP15093
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15093

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 162DHFRPROSITE-ProRule annotationAdd BLAST161

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

eggNOGiarCOG01490 Archaea
COG0262 LUCA
HOGENOMiHOG000040233
KOiK00287
OMAiEECFVIG
OrthoDBiPOG093Z0EES

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
PIRSFiPIRSF000194 DHFR, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P15093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELVSVAALA ENRVIGRDGE LPWPSIPADK KQYRSRIADD PVVLGRTTFE
60 70 80 90 100
SMRDDLPGSA QIVMSRSERS FSVDTAHRAA SVEEAVDIAA SLDAETAYVI
110 120 130 140 150
GGAAIYALFQ PHLDRMVLSR VPGEYEGDTY YPEWDAAEWE LDAETDHEGF
160
TLQEWVRSAS SR
Length:162
Mass (Da):17,980
Last modified:April 1, 1990 - v1
Checksum:i31B047661F14281F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05088 Genomic DNA Translation: AAA72219.1
CP001956 Genomic DNA Translation: ADE04659.1
PIRiA34429
RefSeqiWP_004043660.1, NZ_AOHU01000091.1

Genome annotation databases

EnsemblBacteriaiADE04659; ADE04659; HVO_1279
GeneIDi8925483
KEGGihvo:HVO_1279

Similar proteinsi

Entry informationi

Entry nameiDYRA_HALVD
AccessioniPrimary (citable) accession number: P15093
Secondary accession number(s): D4GXA6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: March 28, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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