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Protein

Mast cell carboxypeptidase A

Gene

Cpa3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi176 – 1761Zinc; catalyticBy similarity
Metal bindingi179 – 1791Zinc; catalyticBy similarity
Metal bindingi304 – 3041Zinc; catalyticBy similarity
Active sitei378 – 3781Proton donor/acceptorBy similarity

GO - Molecular functioni

  • metallocarboxypeptidase activity Source: InterPro
  • peptidase activity Source: MGI
  • zinc ion binding Source: InterPro

GO - Biological processi

  • positive regulation of defense response to virus by host Source: MGI
  • regulation of angiotensin levels in blood Source: MGI
  • xenophagy Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-2022377. Metabolism of Angiotensinogen to Angiotensins.

Protein family/group databases

MEROPSiM14.010.

Names & Taxonomyi

Protein namesi
Recommended name:
Mast cell carboxypeptidase A (EC:3.4.17.1)
Short name:
MC-CPA
Alternative name(s):
Carboxypeptidase A3
Gene namesi
Name:Cpa3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:88479. Cpa3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Add
BLAST
Propeptidei16 – 10994Activation peptidePRO_0000004397Add
BLAST
Chaini110 – 417308Mast cell carboxypeptidase APRO_0000004398Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi173 ↔ 186By similarity
Disulfide bondi245 ↔ 268By similarity

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP15089.
PRIDEiP15089.

PTM databases

PhosphoSiteiP15089.

Expressioni

Gene expression databases

BgeeiP15089.
CleanExiMM_CPA3.
ExpressionAtlasiP15089. baseline and differential.
GenevisibleiP15089. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001921.

Structurei

3D structure databases

ProteinModelPortaliP15089.
SMRiP15089. Positions 21-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
HOGENOMiHOG000252968.
HOVERGENiHBG050815.
InParanoidiP15089.
KOiK08780.
OMAiTMLAVEF.
OrthoDBiEOG7RZ5Q9.
PhylomeDBiP15089.
TreeFamiTF317197.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR000834. Peptidase_M14.
IPR003146. Prot_inh_M14A.
IPR009020. Prot_inh_propept.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15089-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFFLLMAVI YTTLAIAPVH FDREKVFRVK LQNEKHASVL KNLTQSIELD
60 70 80 90 100
FWYPDAIHDI AVNMTVDFRV SEKESQTIQS TLEQHKIHYE ILIHDLQEEI
110 120 130 140 150
EKQFDVKDEI AGRHSYAKYN DWDKIVSWTE KMLEKHPEMV SRIKIGSTVE
160 170 180 190 200
DNPLYVLKIG KKDGERKAIF MDCGIHAREW ISPAFCQWFV YQATKSYGKN
210 220 230 240 250
KIMTKLLDRM NFYVLPVFNV DGYIWSWTQD RMWRKNRSRN QNSTCIGTDL
260 270 280 290 300
NRNFDVSWDS SPNTNKPCLN VYRGPAPESE KETKAVTNFI RSHLNSIKAY
310 320 330 340 350
ITFHSYSQML LIPYGYTFKL PPNHQDLLKV ARIATDALST RYETRYIYGP
360 370 380 390 400
IASTIYKTSG SSLDWVYDLG IKHTFAFELR DKGKSGFLLP ESRIKPTCKE
410
TMLSVKFIAK YILKNTS
Length:417
Mass (Da):48,790
Last modified:April 1, 1990 - v1
Checksum:iA2B300A068D1BA6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05118 mRNA. Translation: AAA37369.1.
CCDSiCCDS17262.1.
PIRiA34487.
RefSeqiNP_031779.1. NM_007753.2.
UniGeneiMm.1135.

Genome annotation databases

EnsembliENSMUST00000001921; ENSMUSP00000001921; ENSMUSG00000001865.
GeneIDi12873.
KEGGimmu:12873.
UCSCiuc008osp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05118 mRNA. Translation: AAA37369.1.
CCDSiCCDS17262.1.
PIRiA34487.
RefSeqiNP_031779.1. NM_007753.2.
UniGeneiMm.1135.

3D structure databases

ProteinModelPortaliP15089.
SMRiP15089. Positions 21-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000001921.

Protein family/group databases

MEROPSiM14.010.

PTM databases

PhosphoSiteiP15089.

Proteomic databases

PaxDbiP15089.
PRIDEiP15089.

Protocols and materials databases

DNASUi12873.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001921; ENSMUSP00000001921; ENSMUSG00000001865.
GeneIDi12873.
KEGGimmu:12873.
UCSCiuc008osp.2. mouse.

Organism-specific databases

CTDi1359.
MGIiMGI:88479. Cpa3.

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
HOGENOMiHOG000252968.
HOVERGENiHBG050815.
InParanoidiP15089.
KOiK08780.
OMAiTMLAVEF.
OrthoDBiEOG7RZ5Q9.
PhylomeDBiP15089.
TreeFamiTF317197.

Enzyme and pathway databases

ReactomeiR-MMU-2022377. Metabolism of Angiotensinogen to Angiotensins.

Miscellaneous databases

PROiP15089.
SOURCEiSearch...

Gene expression databases

BgeeiP15089.
CleanExiMM_CPA3.
ExpressionAtlasiP15089. baseline and differential.
GenevisibleiP15089. MM.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR000834. Peptidase_M14.
IPR003146. Prot_inh_M14A.
IPR009020. Prot_inh_propept.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and molecular cloning of mast cell carboxypeptidase A. A novel member of the carboxypeptidase gene family."
    Reynolds D.S., Stevens R.L., Gurley D.S., Lane W.S., Austen K.F., Serafin W.E.
    J. Biol. Chem. 264:20094-20099(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiCBPA3_MOUSE
AccessioniPrimary (citable) accession number: P15089
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 8, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.