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Protein

Carboxypeptidase E

Gene

Cpe

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing.

Miscellaneous

An autoantigen recognized by serum of pretype I diabetes.

Catalytic activityi

Release of C-terminal arginine or lysine residues from polypeptides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi114Zinc; catalyticBy similarity1
Metal bindingi117Zinc; catalyticBy similarity1
Metal bindingi248Zinc; catalyticBy similarity1
Active sitei342Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • carboxypeptidase activity Source: RGD
  • cobalt ion binding Source: RGD
  • metallocarboxypeptidase activity Source: Reactome
  • protein domain specific binding Source: RGD
  • serine-type carboxypeptidase activity Source: GO_Central
  • zinc ion binding Source: InterPro

GO - Biological processi

  • enkephalin processing Source: RGD
  • insulin processing Source: RGD
  • negative regulation of branching morphogenesis of a nerve Source: RGD
  • peptide catabolic process Source: RGD
  • peptide hormone processing Source: RGD
  • peptide metabolic process Source: RGD
  • protein processing Source: RGD

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM14.005

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase E (EC:3.4.17.10)
Short name:
CPE
Alternative name(s):
Carboxypeptidase H
Short name:
CPH
Enkephalin convertase
Prohormone-processing carboxypeptidase
Gene namesi
Name:Cpe
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2394 Cpe

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Or 34Add BLAST27
PropeptideiPRO_000000438828 – 42Activation peptideAdd BLAST15
ChainiPRO_000000438943 – 476Carboxypeptidase EAdd BLAST434

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi139N-linked (GlcNAc...) asparagine; alternateSequence analysis1
Glycosylationi139N-linked (HexNAc...) asparagine; alternateCombined sources1
Glycosylationi390N-linked (GlcNAc...) asparagine; alternateSequence analysis1
Glycosylationi390N-linked (HexNAc...) asparagine; alternateCombined sources1

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, Zymogen

Proteomic databases

PRIDEiP15087

PTM databases

UniCarbKBiP15087

Expressioni

Tissue specificityi

Expressed in brain, heart and anterior pituitary gland. Also expressed in pancreatic islets and adrenal gland.1 Publication

Interactioni

GO - Molecular functioni

  • protein domain specific binding Source: RGD

Structurei

3D structure databases

ProteinModelPortaliP15087
SMRiP15087
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG003410
InParanoidiP15087
KOiK01294
PhylomeDBiP15087

Family and domain databases

CDDicd03865 M14_CPE, 1 hit
InterProiView protein in InterPro
IPR008969 CarboxyPept-like_regulatory
IPR034232 M14_CPE_CPD
IPR000834 Peptidase_M14
PfamiView protein in Pfam
PF00246 Peptidase_M14, 1 hit
PRINTSiPR00765 CRBOXYPTASEA
SMARTiView protein in SMART
SM00631 Zn_pept, 1 hit
SUPFAMiSSF49464 SSF49464, 1 hit
PROSITEiView protein in PROSITE
PS00132 CARBOXYPEPT_ZN_1, 1 hit
PS00133 CARBOXYPEPT_ZN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRGGRVLL ALCAALVAGG WLLAAEAQEP GAPAAGMRRR RRLQQEDGIS
60 70 80 90 100
FEYHRYPELR EALVSVWLQC TAISRIYTVG RSFEGRELLV IELSDNPGVH
110 120 130 140 150
EPGEPEFKYI GNMHGNEAVG RELLIFLAQY LCNEYQRGNE TIVNLIHSTR
160 170 180 190 200
IHIMPSLNPD GFEKAASQPG ELKDWFVGRS NAQGIDLNRN FPDLDRIVYV
210 220 230 240 250
NEKEGGPNNH LLKNLKKIVD QNSKLAPETK AVIHWIMDIP FVLSANLHGG
260 270 280 290 300
DLVANYPYDE TRSGTAHEYS SCPDDAIFQS LARAYSSFNP VMSDPNRPPC
310 320 330 340 350
RKNDDDSSFV DGTTNGGAWY SVPGGMQDFN YLSSNCFEIT VELSCEKFPP
360 370 380 390 400
EETLKSYWED NKNSLINYLE QIHRGVKGFV RDLQGNPIAN ATISVDGIDH
410 420 430 440 450
DVTSAKDGDY WRLLVPGNYK LTASAPGYLA ITKKVAVPFS PAVGVDFELE
460 470
SFSERKEEEK EELMEWWKMM SETLNF
Length:476
Mass (Da):53,309
Last modified:April 1, 1990 - v1
Checksum:iC2213D1FD6ECA120
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti82S → T in AAA40873 (PubMed:2725530).Curated1
Sequence conflicti415V → A in CAA35768 (PubMed:2334405).Curated1
Sequence conflicti453S → Y in CAA35768 (PubMed:2334405).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04625 mRNA Translation: AAA40875.1
M31602 mRNA Translation: AAA40873.1
X51406 mRNA Translation: CAA35768.1
L07281
, L07273, L07274, L07275, L07277, L07278, L07279, L07280 Genomic DNA Translation: AAA40957.1 Sequence problems.
PIRiA40469
S12461
RefSeqiNP_037260.1, NM_013128.1
UniGeneiRn.7149

Genome annotation databases

GeneIDi25669
KEGGirno:25669

Similar proteinsi

Entry informationi

Entry nameiCBPE_RAT
AccessioniPrimary (citable) accession number: P15087
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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