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P15086

- CBPB1_HUMAN

UniProt

P15086 - CBPB1_HUMAN

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Protein

Carboxypeptidase B

Gene

CPB1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential release of a C-terminal lysine or arginine amino acid.

Cofactori

Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi176 – 1761Zinc
Metal bindingi179 – 1791Zinc
Metal bindingi304 – 3041Zinc
Active sitei378 – 3781NucleophileBy similarity

GO - Molecular functioni

  1. carboxypeptidase activity Source: ProtInc
  2. metallocarboxypeptidase activity Source: InterPro
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_147707. Metabolism of Angiotensinogen to Angiotensins.

Protein family/group databases

MEROPSiM14.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase B (EC:3.4.17.2)
Alternative name(s):
Pancreas-specific protein
Short name:
PASP
Gene namesi
Name:CPB1
Synonyms:CPB, PCPB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:2299. CPB1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26821.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 15152 PublicationsAdd
BLAST
Propeptidei16 – 11095Activation peptidePRO_0000004371Add
BLAST
Chaini111 – 417307Carboxypeptidase BPRO_0000004372Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi173 ↔ 186
Disulfide bondi245 ↔ 268
Disulfide bondi259 ↔ 273

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

MaxQBiP15086.
PaxDbiP15086.
PRIDEiP15086.

Miscellaneous databases

PMAP-CutDBP15086.

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

BgeeiP15086.
CleanExiHS_CPB1.
ExpressionAtlasiP15086. baseline.
GenevestigatoriP15086.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000282957.

Structurei

Secondary structure

1
417
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi19 – 224
Beta strandi26 – 327
Helixi35 – 4713
Beta strandi50 – 578
Helixi58 – 603
Beta strandi63 – 719
Helixi73 – 753
Helixi76 – 8510
Beta strandi90 – 956
Helixi97 – 1037
Beta strandi111 – 1133
Helixi122 – 13514
Turni137 – 1393
Beta strandi140 – 1478
Beta strandi153 – 1608
Beta strandi168 – 1725
Helixi181 – 19616
Turni197 – 1993
Helixi201 – 2099
Beta strandi211 – 2166
Helixi220 – 2289
Helixi250 – 2523
Beta strandi254 – 2574
Beta strandi260 – 2623
Helixi281 – 29212
Turni293 – 2964
Beta strandi297 – 3048
Beta strandi309 – 3135
Beta strandi315 – 3184
Helixi324 – 34219
Beta strandi347 – 3504
Helixi351 – 3544
Helixi362 – 3687
Beta strandi372 – 3787
Beta strandi382 – 3854
Helixi386 – 3883
Helixi391 – 3933
Helixi394 – 41320

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KWMX-ray1.60A/B16-417[»]
1ZLIX-ray2.09A109-417[»]
ProteinModelPortaliP15086.
SMRiP15086. Positions 16-417.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15086.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2866.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252968.
HOVERGENiHBG050815.
InParanoidiP15086.
KOiK01291.
OMAiYEKYNKW.
OrthoDBiEOG7RZ5Q9.
PhylomeDBiP15086.
TreeFamiTF317197.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR000834. Peptidase_M14.
IPR003146. Prot_inh_M14A.
IPR009020. Prot_inh_propept.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15086-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLALLVLVTV ALASAHHGGE HFEGEKVFRV NVEDENHINI IRELASTTQI
60 70 80 90 100
DFWKPDSVTQ IKPHSTVDFR VKAEDTVTVE NVLKQNELQY KVLISNLRNV
110 120 130 140 150
VEAQFDSRVR ATGHSYEKYN KWETIEAWTQ QVATENPALI SRSVIGTTFE
160 170 180 190 200
GRAIYLLKVG KAGQNKPAIF MDCGFHAREW ISPAFCQWFV REAVRTYGRE
210 220 230 240 250
IQVTELLDKL DFYVLPVLNI DGYIYTWTKS RFWRKTRSTH TGSSCIGTDP
260 270 280 290 300
NRNFDAGWCE IGASRNPCDE TYCGPAAESE KETKALADFI RNKLSSIKAY
310 320 330 340 350
LTIHSYSQMM IYPYSYAYKL GENNAELNAL AKATVKELAS LHGTKYTYGP
360 370 380 390 400
GATTIYPAAG GSDDWAYDQG IRYSFTFELR DTGRYGFLLP ESQIRATCEE
410
TFLAIKYVAS YVLEHLY
Length:417
Mass (Da):47,368
Last modified:May 10, 2002 - v4
Checksum:iEBBB98B27F5D5AF9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161H → A AA sequence (PubMed:1370825)Curated
Sequence conflicti17 – 171H → Q AA sequence (PubMed:1370825)Curated
Sequence conflicti37 – 371H → Q AA sequence (PubMed:2920728)Curated
Sequence conflicti245 – 2451Missing in AAA66973. (PubMed:1370825)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti208 – 2081D → N.1 Publication
Corresponds to variant rs1059502 [ dbSNP | Ensembl ].
VAR_048598

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M81057 mRNA. Translation: AAA66973.1.
AJ224866 mRNA. Translation: CAA12163.1.
BT009910 mRNA. Translation: AAP88912.1.
CH471052 Genomic DNA. Translation: EAW78903.1.
BC015338 mRNA. Translation: AAH15338.1.
CCDSiCCDS33874.1.
PIRiA42332.
RefSeqiNP_001862.2. NM_001871.2.
XP_005247181.1. XM_005247124.1.
UniGeneiHs.477891.

Genome annotation databases

EnsembliENST00000282957; ENSP00000282957; ENSG00000153002.
ENST00000491148; ENSP00000417222; ENSG00000153002.
GeneIDi1360.
KEGGihsa:1360.
UCSCiuc003ewl.3. human.

Polymorphism databases

DMDMi20532382.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M81057 mRNA. Translation: AAA66973.1 .
AJ224866 mRNA. Translation: CAA12163.1 .
BT009910 mRNA. Translation: AAP88912.1 .
CH471052 Genomic DNA. Translation: EAW78903.1 .
BC015338 mRNA. Translation: AAH15338.1 .
CCDSi CCDS33874.1.
PIRi A42332.
RefSeqi NP_001862.2. NM_001871.2.
XP_005247181.1. XM_005247124.1.
UniGenei Hs.477891.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1KWM X-ray 1.60 A/B 16-417 [» ]
1ZLI X-ray 2.09 A 109-417 [» ]
ProteinModelPortali P15086.
SMRi P15086. Positions 16-417.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9606.ENSP00000282957.

Chemistry

BindingDBi P15086.
ChEMBLi CHEMBL2552.

Protein family/group databases

MEROPSi M14.003.

Polymorphism databases

DMDMi 20532382.

Proteomic databases

MaxQBi P15086.
PaxDbi P15086.
PRIDEi P15086.

Protocols and materials databases

DNASUi 1360.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000282957 ; ENSP00000282957 ; ENSG00000153002 .
ENST00000491148 ; ENSP00000417222 ; ENSG00000153002 .
GeneIDi 1360.
KEGGi hsa:1360.
UCSCi uc003ewl.3. human.

Organism-specific databases

CTDi 1360.
GeneCardsi GC03P148508.
HGNCi HGNC:2299. CPB1.
MIMi 114852. gene.
neXtProti NX_P15086.
PharmGKBi PA26821.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG2866.
GeneTreei ENSGT00760000119103.
HOGENOMi HOG000252968.
HOVERGENi HBG050815.
InParanoidi P15086.
KOi K01291.
OMAi YEKYNKW.
OrthoDBi EOG7RZ5Q9.
PhylomeDBi P15086.
TreeFami TF317197.

Enzyme and pathway databases

Reactomei REACT_147707. Metabolism of Angiotensinogen to Angiotensins.

Miscellaneous databases

ChiTaRSi CPB1. human.
EvolutionaryTracei P15086.
GenomeRNAii 1360.
NextBioi 5509.
PMAP-CutDB P15086.
PROi P15086.
SOURCEi Search...

Gene expression databases

Bgeei P15086.
CleanExi HS_CPB1.
ExpressionAtlasi P15086. baseline.
Genevestigatori P15086.

Family and domain databases

Gene3Di 3.30.70.340. 1 hit.
InterProi IPR000834. Peptidase_M14.
IPR003146. Prot_inh_M14A.
IPR009020. Prot_inh_propept.
[Graphical view ]
Pfami PF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view ]
PRINTSi PR00765. CRBOXYPTASEA.
SMARTi SM00631. Zn_pept. 1 hit.
[Graphical view ]
SUPFAMi SSF54897. SSF54897. 1 hit.
PROSITEi PS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of a cDNA encoding a human serum marker for acute pancreatitis. Identification of pancreas-specific protein as pancreatic procarboxypeptidase B."
    Yamamoto K.K., Pousette A., Chow P., Wilson H., el Shami S., French C.K.
    J. Biol. Chem. 267:2575-2581(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 16-40.
    Tissue: Pancreas.
  2. "Comparative analysis of the sequences and three-dimensional models of human procarboxypeptidases A1, A2 and B."
    Aloy P., Catasus L., Villegas V., Reverter D., Vendrell J., Aviles F.X.
    Biol. Chem. 379:149-155(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASN-208.
    Tissue: Pancreas.
  3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Adrenal gland.
  6. "Purification and properties of five different forms of human procarboxypeptidases."
    Pascual R., Burgos F.J., Salva M., Soriano F., Mendez E., Aviles F.X.
    Eur. J. Biochem. 179:609-616(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-43.
    Tissue: Pancreas.
  7. "Human procarboxypeptidase B: three-dimensional structure and implications for thrombin-activatable fibrinolysis inhibitor (TAFI)."
    Barbosa-Pereira P.J., Segura-Martin S., Oliva B., Ferrer-Orta C., Aviles F.X., Coll M., Gomis-Ruth F.X., Vendrell J.
    J. Mol. Biol. 321:537-547(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 16-417.

Entry informationi

Entry nameiCBPB1_HUMAN
AccessioniPrimary (citable) accession number: P15086
Secondary accession number(s): O60834, Q53XJ0, Q96BQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: May 10, 2002
Last modified: October 29, 2014
This is version 160 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Peptidase families
    Classification of peptidase families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3