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Protein

Carboxypeptidase B

Gene

CPB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential release of a C-terminal lysine or arginine amino acid.By similarity

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi176Zinc; catalytic1 Publication1
Metal bindingi179Zinc; catalytic1 Publication1
Binding sitei234SubstrateBy similarity1
Metal bindingi304Zinc; catalytic1 Publication1
Binding sitei356SubstrateBy similarity1
Active sitei378Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • carboxypeptidase activity Source: ProtInc
  • metallocarboxypeptidase activity Source: GO_Central
  • zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS07870-MONOMER.
BRENDAi3.4.17.2. 2681.
ReactomeiR-HSA-2022377. Metabolism of Angiotensinogen to Angiotensins.

Protein family/group databases

MEROPSiM14.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase B (EC:3.4.17.2By similarity)
Alternative name(s):
Pancreas-specific protein
Short name:
PASP
Gene namesi
Name:CPB1
Synonyms:CPB, PCPB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:2299. CPB1.

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi1360.
OpenTargetsiENSG00000153002.
PharmGKBiPA26821.

Chemistry databases

ChEMBLiCHEMBL2552.
GuidetoPHARMACOLOGYi1593.

Polymorphism and mutation databases

BioMutaiCPB1.
DMDMi20532382.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 152 PublicationsAdd BLAST15
PropeptideiPRO_000000437116 – 110Activation peptideAdd BLAST95
ChainiPRO_0000004372111 – 417Carboxypeptidase BAdd BLAST307

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi173 ↔ 1861 Publication
Disulfide bondi245 ↔ 2681 Publication
Disulfide bondi259 ↔ 2731 Publication

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

MaxQBiP15086.
PaxDbiP15086.
PeptideAtlasiP15086.
PRIDEiP15086.

PTM databases

PhosphoSitePlusiP15086.

Miscellaneous databases

PMAP-CutDBP15086.

Expressioni

Tissue specificityi

Pancreas.1 Publication

Gene expression databases

BgeeiENSG00000153002.
CleanExiHS_CPB1.
ExpressionAtlasiP15086. baseline and differential.
GenevisibleiP15086. HS.

Organism-specific databases

HPAiCAB026158.
HPA038069.
HPA046340.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000282957.

Chemistry databases

BindingDBiP15086.

Structurei

Secondary structure

1417
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi19 – 22Combined sources4
Beta strandi26 – 32Combined sources7
Helixi35 – 47Combined sources13
Beta strandi50 – 57Combined sources8
Helixi58 – 60Combined sources3
Beta strandi63 – 71Combined sources9
Helixi73 – 75Combined sources3
Helixi76 – 85Combined sources10
Beta strandi90 – 95Combined sources6
Helixi97 – 103Combined sources7
Beta strandi111 – 113Combined sources3
Helixi122 – 135Combined sources14
Turni137 – 139Combined sources3
Beta strandi140 – 147Combined sources8
Beta strandi153 – 160Combined sources8
Beta strandi168 – 172Combined sources5
Helixi181 – 196Combined sources16
Turni197 – 199Combined sources3
Helixi201 – 209Combined sources9
Beta strandi211 – 216Combined sources6
Helixi220 – 228Combined sources9
Helixi250 – 252Combined sources3
Beta strandi254 – 257Combined sources4
Beta strandi260 – 262Combined sources3
Helixi281 – 292Combined sources12
Turni293 – 296Combined sources4
Beta strandi297 – 304Combined sources8
Beta strandi309 – 313Combined sources5
Beta strandi315 – 318Combined sources4
Helixi324 – 342Combined sources19
Beta strandi347 – 350Combined sources4
Helixi351 – 354Combined sources4
Helixi362 – 368Combined sources7
Beta strandi372 – 378Combined sources7
Beta strandi382 – 385Combined sources4
Helixi386 – 388Combined sources3
Helixi391 – 393Combined sources3
Helixi394 – 413Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KWMX-ray1.60A/B16-417[»]
1ZLIX-ray2.09A109-417[»]
ProteinModelPortaliP15086.
SMRiP15086.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15086.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni176 – 179Substrate bindingBy similarity4
Regioni251 – 252Substrate bindingBy similarity2
Regioni305 – 306Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252968.
HOVERGENiHBG050815.
InParanoidiP15086.
KOiK01291.
OMAiLAIKYVA.
OrthoDBiEOG091G0HUI.
PhylomeDBiP15086.
TreeFamiTF317197.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15086-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLALLVLVTV ALASAHHGGE HFEGEKVFRV NVEDENHINI IRELASTTQI
60 70 80 90 100
DFWKPDSVTQ IKPHSTVDFR VKAEDTVTVE NVLKQNELQY KVLISNLRNV
110 120 130 140 150
VEAQFDSRVR ATGHSYEKYN KWETIEAWTQ QVATENPALI SRSVIGTTFE
160 170 180 190 200
GRAIYLLKVG KAGQNKPAIF MDCGFHAREW ISPAFCQWFV REAVRTYGRE
210 220 230 240 250
IQVTELLDKL DFYVLPVLNI DGYIYTWTKS RFWRKTRSTH TGSSCIGTDP
260 270 280 290 300
NRNFDAGWCE IGASRNPCDE TYCGPAAESE KETKALADFI RNKLSSIKAY
310 320 330 340 350
LTIHSYSQMM IYPYSYAYKL GENNAELNAL AKATVKELAS LHGTKYTYGP
360 370 380 390 400
GATTIYPAAG GSDDWAYDQG IRYSFTFELR DTGRYGFLLP ESQIRATCEE
410
TFLAIKYVAS YVLEHLY
Length:417
Mass (Da):47,368
Last modified:May 10, 2002 - v4
Checksum:iEBBB98B27F5D5AF9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16H → A AA sequence (PubMed:1370825).Curated1
Sequence conflicti17H → Q AA sequence (PubMed:1370825).Curated1
Sequence conflicti37H → Q AA sequence (PubMed:2920728).Curated1
Sequence conflicti245Missing in AAA66973 (PubMed:1370825).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048598208D → N.1 PublicationCorresponds to variant rs1059502dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81057 mRNA. Translation: AAA66973.1.
AJ224866 mRNA. Translation: CAA12163.1.
BT009910 mRNA. Translation: AAP88912.1.
CH471052 Genomic DNA. Translation: EAW78903.1.
BC015338 mRNA. Translation: AAH15338.1.
CCDSiCCDS33874.1.
PIRiA42332.
RefSeqiNP_001862.2. NM_001871.2.
UniGeneiHs.477891.

Genome annotation databases

EnsembliENST00000282957; ENSP00000282957; ENSG00000153002.
ENST00000491148; ENSP00000417222; ENSG00000153002.
GeneIDi1360.
KEGGihsa:1360.
UCSCiuc003ewl.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81057 mRNA. Translation: AAA66973.1.
AJ224866 mRNA. Translation: CAA12163.1.
BT009910 mRNA. Translation: AAP88912.1.
CH471052 Genomic DNA. Translation: EAW78903.1.
BC015338 mRNA. Translation: AAH15338.1.
CCDSiCCDS33874.1.
PIRiA42332.
RefSeqiNP_001862.2. NM_001871.2.
UniGeneiHs.477891.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KWMX-ray1.60A/B16-417[»]
1ZLIX-ray2.09A109-417[»]
ProteinModelPortaliP15086.
SMRiP15086.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000282957.

Chemistry databases

BindingDBiP15086.
ChEMBLiCHEMBL2552.
GuidetoPHARMACOLOGYi1593.

Protein family/group databases

MEROPSiM14.003.

PTM databases

PhosphoSitePlusiP15086.

Polymorphism and mutation databases

BioMutaiCPB1.
DMDMi20532382.

Proteomic databases

MaxQBiP15086.
PaxDbiP15086.
PeptideAtlasiP15086.
PRIDEiP15086.

Protocols and materials databases

DNASUi1360.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000282957; ENSP00000282957; ENSG00000153002.
ENST00000491148; ENSP00000417222; ENSG00000153002.
GeneIDi1360.
KEGGihsa:1360.
UCSCiuc003ewl.4. human.

Organism-specific databases

CTDi1360.
DisGeNETi1360.
GeneCardsiCPB1.
HGNCiHGNC:2299. CPB1.
HPAiCAB026158.
HPA038069.
HPA046340.
MIMi114852. gene.
neXtProtiNX_P15086.
OpenTargetsiENSG00000153002.
PharmGKBiPA26821.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252968.
HOVERGENiHBG050815.
InParanoidiP15086.
KOiK01291.
OMAiLAIKYVA.
OrthoDBiEOG091G0HUI.
PhylomeDBiP15086.
TreeFamiTF317197.

Enzyme and pathway databases

BioCyciZFISH:HS07870-MONOMER.
BRENDAi3.4.17.2. 2681.
ReactomeiR-HSA-2022377. Metabolism of Angiotensinogen to Angiotensins.

Miscellaneous databases

ChiTaRSiCPB1. human.
EvolutionaryTraceiP15086.
GenomeRNAii1360.
PMAP-CutDBP15086.
PROiP15086.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000153002.
CleanExiHS_CPB1.
ExpressionAtlasiP15086. baseline and differential.
GenevisibleiP15086. HS.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBPB1_HUMAN
AccessioniPrimary (citable) accession number: P15086
Secondary accession number(s): O60834, Q53XJ0, Q96BQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: May 10, 2002
Last modified: November 2, 2016
This is version 177 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Peptidase families
    Classification of peptidase families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.