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Protein

Carboxypeptidase A1

Gene

CPA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.1 Publication

Catalytic activityi

Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi179Zinc; catalyticCombined sources1 Publication1
Metal bindingi182Zinc; catalyticCombined sources1 Publication1
Binding sitei237SubstrateCombined sources1 Publication1
Metal bindingi306Zinc; catalyticCombined sources1 Publication1
Binding sitei358SubstrateCombined sources1 Publication1
Active sitei380Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • metallocarboxypeptidase activity Source: ProtInc
  • zinc ion binding Source: InterPro

GO - Biological processi

  • proteolysis Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS01745-MONOMER.

Protein family/group databases

MEROPSiM14.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase A1 (EC:3.4.17.1)
Gene namesi
Name:CPA1
Synonyms:CPA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:2296. CPA1.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi1357.
MalaCardsiCPA1.
OpenTargetsiENSG00000091704.
Orphaneti676. Hereditary chronic pancreatitis.
PharmGKBiPA26816.

Chemistry databases

ChEMBLiCHEMBL2088.
GuidetoPHARMACOLOGYi1587.

Polymorphism and mutation databases

BioMutaiCPA1.
DMDMi399196.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 162 PublicationsAdd BLAST16
PropeptideiPRO_000000434517 – 110Activation peptideAdd BLAST94
ChainiPRO_0000004346111 – 419Carboxypeptidase A1Add BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi248 ↔ 2712 Publications

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

MaxQBiP15085.
PaxDbiP15085.
PeptideAtlasiP15085.
PRIDEiP15085.

PTM databases

iPTMnetiP15085.
PhosphoSitePlusiP15085.

Expressioni

Gene expression databases

BgeeiENSG00000091704.
CleanExiHS_CPA1.
ExpressionAtlasiP15085. baseline and differential.
GenevisibleiP15085. HS.

Organism-specific databases

HPAiCAB025197.
HPA021836.
HPA052215.

Interactioni

Subunit structurei

Monomer. May form a complex with proelastase 2.2 Publications

Protein-protein interaction databases

BioGridi107749. 1 interactor.
DIPiDIP-48699N.
IntActiP15085. 1 interactor.
STRINGi9606.ENSP00000011292.

Chemistry databases

BindingDBiP15085.

Structurei

Secondary structure

1419
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 28Combined sources5
Helixi33 – 43Combined sources11
Helixi46 – 48Combined sources3
Beta strandi51 – 54Combined sources4
Beta strandi63 – 67Combined sources5
Helixi69 – 71Combined sources3
Helixi72 – 81Combined sources10
Beta strandi86 – 91Combined sources6
Helixi93 – 104Combined sources12
Turni114 – 116Combined sources3
Helixi125 – 138Combined sources14
Turni140 – 142Combined sources3
Beta strandi143 – 150Combined sources8
Beta strandi156 – 162Combined sources7
Beta strandi164 – 168Combined sources5
Beta strandi171 – 176Combined sources6
Helixi183 – 199Combined sources17
Turni200 – 202Combined sources3
Helixi204 – 212Combined sources9
Beta strandi214 – 219Combined sources6
Helixi223 – 231Combined sources9
Helixi253 – 255Combined sources3
Beta strandi257 – 260Combined sources4
Beta strandi263 – 268Combined sources6
Helixi284 – 296Combined sources13
Beta strandi299 – 306Combined sources8
Beta strandi311 – 315Combined sources5
Helixi326 – 341Combined sources16
Turni342 – 344Combined sources3
Beta strandi349 – 352Combined sources4
Helixi353 – 356Combined sources4
Helixi364 – 370Combined sources7
Beta strandi374 – 380Combined sources7
Beta strandi384 – 387Combined sources4
Helixi393 – 395Combined sources3
Helixi396 – 416Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V77X-ray1.60A/B111-419[»]
3FJUX-ray1.60A112-418[»]
4UEEX-ray2.27A/B111-419[»]
4UEFX-ray1.69A/B111-419[»]
4UEZX-ray2.29A/B111-419[»]
4UF4X-ray1.77A/B1-419[»]
ProteinModelPortaliP15085.
SMRiP15085.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15085.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni179 – 182Substrate bindingCombined sources1 Publication4
Regioni254 – 255Substrate bindingCombined sources1 Publication2
Regioni307 – 308Substrate bindingCombined sources1 Publication2

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252967.
HOVERGENiHBG050815.
InParanoidiP15085.
KOiK08779.
OMAiSHGISYE.
OrthoDBiEOG091G0HUI.
PhylomeDBiP15085.
TreeFamiTF317197.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15085-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGLLVLSVL LGAVFGKEDF VGHQVLRISV ADEAQVQKVK ELEDLEHLQL
60 70 80 90 100
DFWRGPAHPG SPIDVRVPFP SIQAVKIFLE SHGISYETMI EDVQSLLDEE
110 120 130 140 150
QEQMFAFRSR ARSTDTFNYA TYHTLEEIYD FLDLLVAENP HLVSKIQIGN
160 170 180 190 200
TYEGRPIYVL KFSTGGSKRP AIWIDTGIHS REWVTQASGV WFAKKITQDY
210 220 230 240 250
GQDAAFTAIL DTLDIFLEIV TNPDGFAFTH STNRMWRKTR SHTAGSLCIG
260 270 280 290 300
VDPNRNWDAG FGLSGASSNP CSETYHGKFA NSEVEVKSIV DFVKDHGNIK
310 320 330 340 350
AFISIHSYSQ LLMYPYGYKT EPVPDQDELD QLSKAAVTAL ASLYGTKFNY
360 370 380 390 400
GSIIKAIYQA SGSTIDWTYS QGIKYSFTFE LRDTGRYGFL LPASQIIPTA
410
KETWLALLTI MEHTLNHPY
Length:419
Mass (Da):47,140
Last modified:July 1, 1993 - v2
Checksum:i439FAFFFAEE958B1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139 – 141NPH → HPG AA sequence (PubMed:8318831).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048593208A → T.Corresponds to variant rs34474469dbSNPEnsembl.1
Natural variantiVAR_054311276H → R.2 PublicationsCorresponds to variant rs17849959dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67318 mRNA. Translation: CAA47732.1.
AK291493 mRNA. Translation: BAF84182.1.
BT007313 mRNA. Translation: AAP35977.1.
CH236950 Genomic DNA. Translation: EAL24089.1.
CH471070 Genomic DNA. Translation: EAW83763.1.
BC005279 mRNA. Translation: AAH05279.1.
CCDSiCCDS5820.1.
PIRiS29127.
RefSeqiNP_001859.1. NM_001868.3.
UniGeneiHs.2879.

Genome annotation databases

EnsembliENST00000011292; ENSP00000011292; ENSG00000091704.
GeneIDi1357.
KEGGihsa:1357.
UCSCiuc003vpx.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67318 mRNA. Translation: CAA47732.1.
AK291493 mRNA. Translation: BAF84182.1.
BT007313 mRNA. Translation: AAP35977.1.
CH236950 Genomic DNA. Translation: EAL24089.1.
CH471070 Genomic DNA. Translation: EAW83763.1.
BC005279 mRNA. Translation: AAH05279.1.
CCDSiCCDS5820.1.
PIRiS29127.
RefSeqiNP_001859.1. NM_001868.3.
UniGeneiHs.2879.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V77X-ray1.60A/B111-419[»]
3FJUX-ray1.60A112-418[»]
4UEEX-ray2.27A/B111-419[»]
4UEFX-ray1.69A/B111-419[»]
4UEZX-ray2.29A/B111-419[»]
4UF4X-ray1.77A/B1-419[»]
ProteinModelPortaliP15085.
SMRiP15085.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107749. 1 interactor.
DIPiDIP-48699N.
IntActiP15085. 1 interactor.
STRINGi9606.ENSP00000011292.

Chemistry databases

BindingDBiP15085.
ChEMBLiCHEMBL2088.
GuidetoPHARMACOLOGYi1587.

Protein family/group databases

MEROPSiM14.001.

PTM databases

iPTMnetiP15085.
PhosphoSitePlusiP15085.

Polymorphism and mutation databases

BioMutaiCPA1.
DMDMi399196.

Proteomic databases

MaxQBiP15085.
PaxDbiP15085.
PeptideAtlasiP15085.
PRIDEiP15085.

Protocols and materials databases

DNASUi1357.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000011292; ENSP00000011292; ENSG00000091704.
GeneIDi1357.
KEGGihsa:1357.
UCSCiuc003vpx.4. human.

Organism-specific databases

CTDi1357.
DisGeNETi1357.
GeneCardsiCPA1.
HGNCiHGNC:2296. CPA1.
HPAiCAB025197.
HPA021836.
HPA052215.
MalaCardsiCPA1.
MIMi114850. gene.
neXtProtiNX_P15085.
OpenTargetsiENSG00000091704.
Orphaneti676. Hereditary chronic pancreatitis.
PharmGKBiPA26816.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2650. Eukaryota.
COG2866. LUCA.
GeneTreeiENSGT00760000119103.
HOGENOMiHOG000252967.
HOVERGENiHBG050815.
InParanoidiP15085.
KOiK08779.
OMAiSHGISYE.
OrthoDBiEOG091G0HUI.
PhylomeDBiP15085.
TreeFamiTF317197.

Enzyme and pathway databases

BioCyciZFISH:HS01745-MONOMER.

Miscellaneous databases

ChiTaRSiCPA1. human.
EvolutionaryTraceiP15085.
GeneWikiiCarboxypeptidase_A1.
GenomeRNAii1357.
PROiP15085.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000091704.
CleanExiHS_CPA1.
ExpressionAtlasiP15085. baseline and differential.
GenevisibleiP15085. HS.

Family and domain databases

Gene3Di3.30.70.340. 1 hit.
InterProiIPR003146. M14A_act_pep.
IPR000834. Peptidase_M14.
IPR009020. Propept_inh.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
PF02244. Propep_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF54897. SSF54897. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBPA1_HUMAN
AccessioniPrimary (citable) accession number: P15085
Secondary accession number(s): A4D1M1
, Q53XU0, Q9BS67, Q9UCF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 164 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Peptidase families
    Classification of peptidase families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.