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Protein

Glycogen debranching enzyme

Gene

glgX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen.1 Publication

Pathwayi: glycogen degradation

This protein is involved in the pathway glycogen degradation, which is part of Glycan degradation.
View all proteins of this organism that are known to be involved in the pathway glycogen degradation and in Glycan degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei336 – 3361NucleophileBy similarity
Active sitei371 – 3711Proton donorBy similarity
Sitei443 – 4431Transition state stabilizerBy similarity

GO - Molecular functioni

  • amylo-alpha-1,6-glucosidase activity Source: EcoCyc
  • glycogen debranching enzyme activity Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • glycogen catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Enzyme and pathway databases

BioCyciEcoCyc:EG10381-MONOMER.
ECOL316407:JW3394-MONOMER.
MetaCyc:EG10381-MONOMER.
BRENDAi3.2.1.33. 2026.
UniPathwayiUPA00165.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen debranching enzyme (EC:3.2.1.-)
Alternative name(s):
Glycogen operon protein GlgX
Gene namesi
Name:glgX
Synonyms:glyX
Ordered Locus Names:b3431, JW3394
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10381. glgX.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 657657Glycogen debranching enzymePRO_0000054297Add
BLAST

Proteomic databases

PaxDbiP15067.

Interactioni

Protein-protein interaction databases

BioGridi4263517. 16 interactions.
IntActiP15067. 14 interactions.
STRINGi511145.b3431.

Structurei

Secondary structure

1
657
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 166Combined sources
Beta strandi18 – 269Combined sources
Beta strandi31 – 388Combined sources
Beta strandi44 – 485Combined sources
Beta strandi51 – 533Combined sources
Beta strandi56 – 627Combined sources
Beta strandi69 – 757Combined sources
Helixi80 – 823Combined sources
Beta strandi100 – 1023Combined sources
Helixi108 – 1103Combined sources
Beta strandi114 – 1163Combined sources
Helixi123 – 1253Combined sources
Beta strandi128 – 1303Combined sources
Helixi148 – 1503Combined sources
Beta strandi153 – 1564Combined sources
Helixi158 – 1625Combined sources
Helixi170 – 1723Combined sources
Helixi176 – 1794Combined sources
Helixi182 – 19110Combined sources
Beta strandi195 – 1995Combined sources
Beta strandi202 – 2043Combined sources
Helixi208 – 2114Combined sources
Turni212 – 2143Combined sources
Beta strandi223 – 2297Combined sources
Helixi231 – 2333Combined sources
Helixi237 – 2393Combined sources
Helixi240 – 25314Combined sources
Beta strandi257 – 2626Combined sources
Helixi279 – 2879Combined sources
Beta strandi294 – 2963Combined sources
Beta strandi299 – 3035Combined sources
Helixi311 – 32616Combined sources
Beta strandi332 – 3354Combined sources
Turni336 – 3383Combined sources
Helixi339 – 3424Combined sources
Beta strandi343 – 3475Combined sources
Helixi352 – 3598Combined sources
Helixi363 – 3653Combined sources
Beta strandi366 – 3705Combined sources
Beta strandi389 – 3924Combined sources
Helixi394 – 40512Combined sources
Helixi411 – 4188Combined sources
Helixi422 – 4254Combined sources
Helixi432 – 4343Combined sources
Beta strandi435 – 4373Combined sources
Beta strandi442 – 4443Combined sources
Helixi447 – 4504Combined sources
Helixi459 – 4613Combined sources
Beta strandi479 – 4824Combined sources
Helixi486 – 50520Combined sources
Beta strandi506 – 5138Combined sources
Turni514 – 5196Combined sources
Turni534 – 5363Combined sources
Helixi540 – 5423Combined sources
Helixi545 – 55814Combined sources
Helixi562 – 5654Combined sources
Beta strandi574 – 5807Combined sources
Beta strandi584 – 5863Combined sources
Helixi589 – 5946Combined sources
Beta strandi597 – 6037Combined sources
Turni604 – 6063Combined sources
Beta strandi607 – 6126Combined sources
Beta strandi614 – 6163Combined sources
Beta strandi618 – 6214Combined sources
Beta strandi643 – 6475Combined sources
Beta strandi651 – 6566Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WSKX-ray2.25A1-657[»]
ProteinModelPortaliP15067.
SMRiP15067. Positions 4-657.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15067.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiENOG4107SS7. Bacteria.
COG1523. LUCA.
HOGENOMiHOG000239197.
InParanoidiP15067.
KOiK02438.
OMAiYSYNHGV.
OrthoDBiEOG64BQ3X.
PhylomeDBiP15067.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01248. GlgX.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR022844. Glycogen_debranch_bac.
IPR011837. Glycogen_debranch_GlgX.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02100. glgX_debranch. 1 hit.

Sequencei

Sequence statusi: Complete.

P15067-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQLAIGKPA PLGAHYDGQG VNFTLFSAHA ERVELCVFDA NGQEHRYDLP
60 70 80 90 100
GHSGDIWHGY LPDARPGLRY GYRVHGPWQP AEGHRFNPAK LLIDPCARQI
110 120 130 140 150
DGEFKDNPLL HAGHNEPDYR DNAAIAPKCV VVVDHYDWED DAPPRTPWGS
160 170 180 190 200
TIIYEAHVKG LTYLHPEIPV EIRGTYKALG HPVMINYLKQ LGITALELLP
210 220 230 240 250
VAQFASEPRL QRMGLSNYWG YNPVAMFALH PAYACSPETA LDEFRDAIKA
260 270 280 290 300
LHKAGIEVIL DIVLNHSAEL DLDGPLFSLR GIDNRSYYWI REDGDYHNWT
310 320 330 340 350
GCGNTLNLSH PAVVDYASAC LRYWVETCHV DGFRFDLAAV MGRTPEFRQD
360 370 380 390 400
APLFTAIQNC PVLSQVKLIA EPWDIAPGGY QVGNFPPLFA EWNDHFRDAA
410 420 430 440 450
RRFWLHYDLP LGAFAGRFAA SSDVFKRNGR LPSAAINLVT AHDGFTLRDC
460 470 480 490 500
VCFNHKHNEA NGEENRDGTN NNYSNNHGKE GLGGSLDLVE RRRDSIHALL
510 520 530 540 550
TTLLLSQGTP MLLAGDEHGH SQHGNNNAYC QDNQLTWLDW SQASSGLTAF
560 570 580 590 600
TAALIHLRKR IPALVENRWW EEGDGNVRWL NRYAQPLSTD EWQNGPKQLQ
610 620 630 640 650
ILLSDRFLIA INATLEVTEI VLPAGEWHAI PPFAGEDNPV ITAVWQGPAH

GLCVFQR
Length:657
Mass (Da):73,577
Last modified:November 1, 1995 - v3
Checksum:iAA9EFC1F67FD0420
GO

Sequence cautioni

The sequence AAA98735.1 differs from that shown. Reason: Erroneous termination at position 507. Translated as Gln.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti288 – 2881Y → YY in AAA98735 (PubMed:2975249).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01616 Genomic DNA. Translation: AAA98735.1. Sequence problems.
U18997 Genomic DNA. Translation: AAA58229.1.
U00096 Genomic DNA. Translation: AAC76456.1.
AP009048 Genomic DNA. Translation: BAE77861.1.
PIRiB65139. BVECGX.
RefSeqiNP_417889.1. NC_000913.3.
WP_000192523.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76456; AAC76456; b3431.
BAE77861; BAE77861; BAE77861.
GeneIDi947941.
KEGGiecj:JW3394.
eco:b3431.
PATRICi32122302. VBIEscCol129921_3528.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01616 Genomic DNA. Translation: AAA98735.1. Sequence problems.
U18997 Genomic DNA. Translation: AAA58229.1.
U00096 Genomic DNA. Translation: AAC76456.1.
AP009048 Genomic DNA. Translation: BAE77861.1.
PIRiB65139. BVECGX.
RefSeqiNP_417889.1. NC_000913.3.
WP_000192523.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2WSKX-ray2.25A1-657[»]
ProteinModelPortaliP15067.
SMRiP15067. Positions 4-657.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263517. 16 interactions.
IntActiP15067. 14 interactions.
STRINGi511145.b3431.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbiP15067.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76456; AAC76456; b3431.
BAE77861; BAE77861; BAE77861.
GeneIDi947941.
KEGGiecj:JW3394.
eco:b3431.
PATRICi32122302. VBIEscCol129921_3528.

Organism-specific databases

EchoBASEiEB0376.
EcoGeneiEG10381. glgX.

Phylogenomic databases

eggNOGiENOG4107SS7. Bacteria.
COG1523. LUCA.
HOGENOMiHOG000239197.
InParanoidiP15067.
KOiK02438.
OMAiYSYNHGV.
OrthoDBiEOG64BQ3X.
PhylomeDBiP15067.

Enzyme and pathway databases

UniPathwayiUPA00165.
BioCyciEcoCyc:EG10381-MONOMER.
ECOL316407:JW3394-MONOMER.
MetaCyc:EG10381-MONOMER.
BRENDAi3.2.1.33. 2026.

Miscellaneous databases

EvolutionaryTraceiP15067.
PROiP15067.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01248. GlgX.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR022844. Glycogen_debranch_bac.
IPR011837. Glycogen_debranch_GlgX.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02100. glgX_debranch. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Analysis of the Escherichia coli glycogen gene cluster suggests that catabolic enzymes are encoded among the biosynthetic genes."
    Romeo T., Kumar A., Preiss J.
    Gene 70:363-376(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-590.
    Strain: K12.
  4. "Coordinate genetic regulation of glycogen catabolism and biosynthesis in Escherichia coli via the CsrA gene product."
    Yang H., Liu M.-Y., Romeo T.
    J. Bacteriol. 178:1012-1017(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12.

Entry informationi

Entry nameiGLGX_ECOLI
AccessioniPrimary (citable) accession number: P15067
Secondary accession number(s): P76693, Q2M795
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.