Glycogen debranching enzyme - Escherichia coli (strain K12)

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P15067 (GLGX_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glycogen debranching enzyme
EC=3.2.1.-

Alternative name(s):
Glycogen operon protein GlgX

Gene names

Name:	glgX
Synonyms:	glyX
Ordered Locus Names:	b3431, JW3394

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen. Ref.4
Pathway	Glycan degradation; glycogen degradation. HAMAP MF_01248
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.
Sequence caution	The sequence AAA98735.1 differs from that shown. Reason: Erroneous termination at position 507. Translated as Gln.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Molecular function	Glycosidase Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycogen catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glycogen debranching enzyme activity Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 657

657

Glycogen debranching enzyme HAMAP MF_01248

PRO_0000054297

Sites

Active site

336

Nucleophile By similarity

Active site

371

Proton donor By similarity

Site

443

Transition state stabilizer By similarity

Experimental info

Sequence conflict

288

Y → YY in AAA98735. Ref.3

Secondary structure

657

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P15067 [UniParc].

Last modified November 1, 1995. Version 3.
Checksum: AA9EFC1F67FD0420
Show »

FASTA

657

73,577

        10         20         30         40         50         60 
MTQLAIGKPA PLGAHYDGQG VNFTLFSAHA ERVELCVFDA NGQEHRYDLP GHSGDIWHGY 

        70         80         90        100        110        120 
LPDARPGLRY GYRVHGPWQP AEGHRFNPAK LLIDPCARQI DGEFKDNPLL HAGHNEPDYR 

       130        140        150        160        170        180 
DNAAIAPKCV VVVDHYDWED DAPPRTPWGS TIIYEAHVKG LTYLHPEIPV EIRGTYKALG 

       190        200        210        220        230        240 
HPVMINYLKQ LGITALELLP VAQFASEPRL QRMGLSNYWG YNPVAMFALH PAYACSPETA 

       250        260        270        280        290        300 
LDEFRDAIKA LHKAGIEVIL DIVLNHSAEL DLDGPLFSLR GIDNRSYYWI REDGDYHNWT 

       310        320        330        340        350        360 
GCGNTLNLSH PAVVDYASAC LRYWVETCHV DGFRFDLAAV MGRTPEFRQD APLFTAIQNC 

       370        380        390        400        410        420 
PVLSQVKLIA EPWDIAPGGY QVGNFPPLFA EWNDHFRDAA RRFWLHYDLP LGAFAGRFAA 

       430        440        450        460        470        480 
SSDVFKRNGR LPSAAINLVT AHDGFTLRDC VCFNHKHNEA NGEENRDGTN NNYSNNHGKE 

       490        500        510        520        530        540 
GLGGSLDLVE RRRDSIHALL TTLLLSQGTP MLLAGDEHGH SQHGNNNAYC QDNQLTWLDW 

       550        560        570        580        590        600 
SQASSGLTAF TAALIHLRKR IPALVENRWW EEGDGNVRWL NRYAQPLSTD EWQNGPKQLQ 

       610        620        630        640        650 
ILLSDRFLIA INATLEVTEI VLPAGEWHAI PPFAGEDNPV ITAVWQGPAH GLCVFQR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Analysis of the Escherichia coli glycogen gene cluster suggests that catabolic enzymes are encoded among the biosynthetic genes." Romeo T., Kumar A., Preiss J. Gene 70:363-376(1988) [PubMed: 2975249] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-590. Strain: K12.
[4]	"Coordinate genetic regulation of glycogen catabolism and biosynthesis in Escherichia coli via the CsrA gene product." Yang H., Liu M.-Y., Romeo T. J. Bacteriol. 178:1012-1017(1996) [PubMed: 8576033] [Abstract] Cited for: FUNCTION. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J01616 Genomic DNA. Translation: AAA98735.1. Sequence problems.
U18997 Genomic DNA. Translation: AAA58229.1.
U00096 Genomic DNA. Translation: AAC76456.1.
AP009048 Genomic DNA. Translation: BAE77861.1.

PIR

BVECGX. B65139.

RefSeq

NP_417889.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WSK	X-ray	2.25	A	1-657	[»]

ProteinModelPortal

P15067.

SMR

P15067. Positions 4-657.

ModBase

Search...

Protein-protein interaction databases

IntAct

P15067. 8 interactions.

Protein family/group databases

CAZy

CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000000066; EBESCP00000000066; EBESCG00000000052.
EBESCT00000017349; EBESCP00000016640; EBESCG00000016405.

GeneID

947941.

GenomeReviews

Gene locus JW3394 in contig AP009048_GR.
Gene locus b3431 in contig U00096_GR.

KEGG

ecj:JW3394.
eco:b3431.

PATRIC

32122302. VBIEscCol129921_3528.

Organism-specific databases

EchoBASE

EB0376.

EcoGene

EG10381. glgX.

Phylogenomic databases

eggNOG

COG1523.

GeneTree

EBGT00050000009924.

HOGENOM

HBG350946.

OMA

WTGCGNM.

PhylomeDB

P15067.

ProtClustDB

PRK03705.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10381-MONOMER.
MetaCyc:EG10381-MONOMER.

Gene expression databases

Genevestigator

P15067.

Family and domain databases

HAMAP

MF_01248. GlgX.
[Tree]

InterPro

IPR015902. Alpha_amylase.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR022844. Glycogen_debranch_bac.
IPR011837. Glycogen_debranch_debranch.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
G3DSA:2.60.40.10. Ig-like_fold. 1 hit.

K02438.

PANTHER

PTHR10357. Alpha_amylase. 1 hit.
PTHR10357:SF74. PTHR10357:SF74. 1 hit.

Pfam

PF00128. Alpha-amylase. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.
SSF81296. Ig_E-set. 1 hit.

TIGRFAMs

TIGR02100. GlgX_debranch. 1 hit.

ProtoNet

Search...

Entry information

Entry name

GLGX_ECOLI

Accession

Primary (citable) accession number: P15067
Secondary accession number(s): P76693, Q2M795

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 103 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents