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Protein

Glycogen debranching enzyme

Gene

glgX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen.1 Publication

Pathwayi: glycogen degradation

This protein is involved in the pathway glycogen degradation, which is part of Glycan degradation.
View all proteins of this organism that are known to be involved in the pathway glycogen degradation and in Glycan degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei336NucleophileBy similarity1
Active sitei371Proton donorBy similarity1
Sitei443Transition state stabilizerBy similarity1

GO - Molecular functioni

  • amylo-alpha-1,6-glucosidase activity Source: EcoCyc
  • glycogen debranching enzyme activity Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • glycogen catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Enzyme and pathway databases

BioCyciEcoCyc:EG10381-MONOMER.
ECOL316407:JW3394-MONOMER.
MetaCyc:EG10381-MONOMER.
BRENDAi3.2.1.33. 2026.
UniPathwayiUPA00165.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen debranching enzyme (EC:3.2.1.-)
Alternative name(s):
Glycogen operon protein GlgX
Gene namesi
Name:glgX
Synonyms:glyX
Ordered Locus Names:b3431, JW3394
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10381. glgX.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000542971 – 657Glycogen debranching enzymeAdd BLAST657

Proteomic databases

PaxDbiP15067.
PRIDEiP15067.

Interactioni

Protein-protein interaction databases

BioGridi4263517. 16 interactors.
IntActiP15067. 14 interactors.
STRINGi511145.b3431.

Structurei

Secondary structure

1657
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 16Combined sources6
Beta strandi18 – 26Combined sources9
Beta strandi31 – 38Combined sources8
Beta strandi44 – 48Combined sources5
Beta strandi51 – 53Combined sources3
Beta strandi56 – 62Combined sources7
Beta strandi69 – 75Combined sources7
Helixi80 – 82Combined sources3
Beta strandi100 – 102Combined sources3
Helixi108 – 110Combined sources3
Beta strandi114 – 116Combined sources3
Helixi123 – 125Combined sources3
Beta strandi128 – 130Combined sources3
Helixi148 – 150Combined sources3
Beta strandi153 – 156Combined sources4
Helixi158 – 162Combined sources5
Helixi170 – 172Combined sources3
Helixi176 – 179Combined sources4
Helixi182 – 191Combined sources10
Beta strandi195 – 199Combined sources5
Beta strandi202 – 204Combined sources3
Helixi208 – 211Combined sources4
Turni212 – 214Combined sources3
Beta strandi223 – 229Combined sources7
Helixi231 – 233Combined sources3
Helixi237 – 239Combined sources3
Helixi240 – 253Combined sources14
Beta strandi257 – 262Combined sources6
Helixi279 – 287Combined sources9
Beta strandi294 – 296Combined sources3
Beta strandi299 – 303Combined sources5
Helixi311 – 326Combined sources16
Beta strandi332 – 335Combined sources4
Turni336 – 338Combined sources3
Helixi339 – 342Combined sources4
Beta strandi343 – 347Combined sources5
Helixi352 – 359Combined sources8
Helixi363 – 365Combined sources3
Beta strandi366 – 370Combined sources5
Beta strandi389 – 392Combined sources4
Helixi394 – 405Combined sources12
Helixi411 – 418Combined sources8
Helixi422 – 425Combined sources4
Helixi432 – 434Combined sources3
Beta strandi435 – 437Combined sources3
Beta strandi442 – 444Combined sources3
Helixi447 – 450Combined sources4
Helixi459 – 461Combined sources3
Beta strandi479 – 482Combined sources4
Helixi486 – 505Combined sources20
Beta strandi506 – 513Combined sources8
Turni514 – 519Combined sources6
Turni534 – 536Combined sources3
Helixi540 – 542Combined sources3
Helixi545 – 558Combined sources14
Helixi562 – 565Combined sources4
Beta strandi574 – 580Combined sources7
Beta strandi584 – 586Combined sources3
Helixi589 – 594Combined sources6
Beta strandi597 – 603Combined sources7
Turni604 – 606Combined sources3
Beta strandi607 – 612Combined sources6
Beta strandi614 – 616Combined sources3
Beta strandi618 – 621Combined sources4
Beta strandi643 – 647Combined sources5
Beta strandi651 – 656Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WSKX-ray2.25A1-657[»]
ProteinModelPortaliP15067.
SMRiP15067.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15067.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiENOG4107SS7. Bacteria.
COG1523. LUCA.
HOGENOMiHOG000239197.
InParanoidiP15067.
KOiK02438.
OMAiWVEECHI.
PhylomeDBiP15067.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01248. GlgX. 1 hit.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR022844. Glycogen_debranch_bac.
IPR011837. Glycogen_debranch_GlgX.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02100. glgX_debranch. 1 hit.

Sequencei

Sequence statusi: Complete.

P15067-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQLAIGKPA PLGAHYDGQG VNFTLFSAHA ERVELCVFDA NGQEHRYDLP
60 70 80 90 100
GHSGDIWHGY LPDARPGLRY GYRVHGPWQP AEGHRFNPAK LLIDPCARQI
110 120 130 140 150
DGEFKDNPLL HAGHNEPDYR DNAAIAPKCV VVVDHYDWED DAPPRTPWGS
160 170 180 190 200
TIIYEAHVKG LTYLHPEIPV EIRGTYKALG HPVMINYLKQ LGITALELLP
210 220 230 240 250
VAQFASEPRL QRMGLSNYWG YNPVAMFALH PAYACSPETA LDEFRDAIKA
260 270 280 290 300
LHKAGIEVIL DIVLNHSAEL DLDGPLFSLR GIDNRSYYWI REDGDYHNWT
310 320 330 340 350
GCGNTLNLSH PAVVDYASAC LRYWVETCHV DGFRFDLAAV MGRTPEFRQD
360 370 380 390 400
APLFTAIQNC PVLSQVKLIA EPWDIAPGGY QVGNFPPLFA EWNDHFRDAA
410 420 430 440 450
RRFWLHYDLP LGAFAGRFAA SSDVFKRNGR LPSAAINLVT AHDGFTLRDC
460 470 480 490 500
VCFNHKHNEA NGEENRDGTN NNYSNNHGKE GLGGSLDLVE RRRDSIHALL
510 520 530 540 550
TTLLLSQGTP MLLAGDEHGH SQHGNNNAYC QDNQLTWLDW SQASSGLTAF
560 570 580 590 600
TAALIHLRKR IPALVENRWW EEGDGNVRWL NRYAQPLSTD EWQNGPKQLQ
610 620 630 640 650
ILLSDRFLIA INATLEVTEI VLPAGEWHAI PPFAGEDNPV ITAVWQGPAH

GLCVFQR
Length:657
Mass (Da):73,577
Last modified:November 1, 1995 - v3
Checksum:iAA9EFC1F67FD0420
GO

Sequence cautioni

The sequence AAA98735 differs from that shown. Reason: Erroneous termination at position 507. Translated as Gln.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti288Y → YY in AAA98735 (PubMed:2975249).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01616 Genomic DNA. Translation: AAA98735.1. Sequence problems.
U18997 Genomic DNA. Translation: AAA58229.1.
U00096 Genomic DNA. Translation: AAC76456.1.
AP009048 Genomic DNA. Translation: BAE77861.1.
PIRiB65139. BVECGX.
RefSeqiNP_417889.1. NC_000913.3.
WP_000192523.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76456; AAC76456; b3431.
BAE77861; BAE77861; BAE77861.
GeneIDi947941.
KEGGiecj:JW3394.
eco:b3431.
PATRICi32122302. VBIEscCol129921_3528.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01616 Genomic DNA. Translation: AAA98735.1. Sequence problems.
U18997 Genomic DNA. Translation: AAA58229.1.
U00096 Genomic DNA. Translation: AAC76456.1.
AP009048 Genomic DNA. Translation: BAE77861.1.
PIRiB65139. BVECGX.
RefSeqiNP_417889.1. NC_000913.3.
WP_000192523.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WSKX-ray2.25A1-657[»]
ProteinModelPortaliP15067.
SMRiP15067.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263517. 16 interactors.
IntActiP15067. 14 interactors.
STRINGi511145.b3431.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbiP15067.
PRIDEiP15067.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76456; AAC76456; b3431.
BAE77861; BAE77861; BAE77861.
GeneIDi947941.
KEGGiecj:JW3394.
eco:b3431.
PATRICi32122302. VBIEscCol129921_3528.

Organism-specific databases

EchoBASEiEB0376.
EcoGeneiEG10381. glgX.

Phylogenomic databases

eggNOGiENOG4107SS7. Bacteria.
COG1523. LUCA.
HOGENOMiHOG000239197.
InParanoidiP15067.
KOiK02438.
OMAiWVEECHI.
PhylomeDBiP15067.

Enzyme and pathway databases

UniPathwayiUPA00165.
BioCyciEcoCyc:EG10381-MONOMER.
ECOL316407:JW3394-MONOMER.
MetaCyc:EG10381-MONOMER.
BRENDAi3.2.1.33. 2026.

Miscellaneous databases

EvolutionaryTraceiP15067.
PROiP15067.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01248. GlgX. 1 hit.
InterProiIPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR022844. Glycogen_debranch_bac.
IPR011837. Glycogen_debranch_GlgX.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02100. glgX_debranch. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLGX_ECOLI
AccessioniPrimary (citable) accession number: P15067
Secondary accession number(s): P76693, Q2M795
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.