2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P15047 (ENTA_ECOLI)

Last modified June 16, 2009. Version 83. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
EC=1.3.1.28

Gene names

Name:	entA
Ordered Locus Names:	b0596, JW0588

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	2,3-dihydro-2,3-dihydroxybenzoate + NAD⁺ = 2,3-dihydroxybenzoate + NADH.
Pathway	Siderophore biosynthesis; enterobactin biosynthesis.
Subunit structure	Homooctamer.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

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Ontologies

Keywords
Biological process	Enterobactin biosynthesis Ion transport Iron transport Transport
Ligand	Iron NAD
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	enterobactin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW iron ion transport Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 248

248

2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase

PRO_0000054659

Regions

Nucleotide binding

9 – 33

NAD By similarity

Sites

Active site

144

Proton acceptor By similarity

Binding site

131

Substrate By similarity

Secondary structure

248

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P15047-1 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: E840488335AD317B
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FASTA

248

26,250

        10         20         30         40         50         60 
MDFSGKNVWV TGAGKGIGYA TALAFVEAGA KVTGFDQAFT QEQYPFATEV MDVADAAQVA 

        70         80         90        100        110        120 
QVCQRLLAET ERLDALVNAA GILRMGATDQ LSKEDWQQTF AVNVGGAFNL FQQTMNQFRR 

       130        140        150        160        170        180 
QRGGAIVTVA SDAAHTPRIG MSAYGASKAA LKSLALSVGL ELAGSGVRCN VVSPGSTDTD 

       190        200        210        220        230        240 
MQRTLWVSDD AEEQRIRGFG EQFKLGIPLG KIARPQEIAN TILFLASDLA SHITLQDIVV 


DGGSTLGA

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase." Liu J., Duncan K., Walsh C.T. J. Bacteriol. 171:791-798(1989) [PubMed: 2521622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-14.
[2]	"Nucleotide sequence and transcriptional organization of the Escherichia coli enterobactin biosynthesis cistrons entB and entA." Nahlik M.S., Brickman T.J., Ozenberger B.A., McIntosh M.A. J. Bacteriol. 171:784-790(1989) [PubMed: 2521621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

M24148 Unassigned DNA. Translation: AAA16103.1.
M24143 Genomic DNA. Translation: AAA76836.1.
U82598 Genomic DNA. Translation: AAB40796.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73697.1.
AP009048 Genomic DNA. Translation: BAE76351.1.

PIR

DEECDB. A91904.

RefSeq

AP_001243.1.
NP_415128.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FWM	X-ray	2.00	X	1-248	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:9511N.

Genome annotation databases

GeneID

945284.

GenomeReviews

Gene locus JW0588 in contig AP009048_GR.
Gene locus b0596 in contig U00096_GR.

KEGG

ecj:JW0588.
eco:b0596.

Organism-specific databases

EchoBASE

EB0255.

EcoGene

EG10259. entA.

CMR

Search...

Phylogenomic databases

HOGENOM

P15047.

OMA

P15047. HASHITL.

Enzyme and pathway databases

BioCyc

EcoCyc:ENTA-MON.
MetaCyc:ENTA-MON.

Family and domain databases

InterPro

IPR002198. DH_sc/Rdtase_SDR.
IPR003560. DHB_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR19410. ADH_short_C2. 1 hit.

Pfam

PF00106. adh_short. 1 hit.
[Graphical view]

PRINTS

PR01397. DHBDHDRGNASE.
PR00080. SDRFAMILY.

PROSITE

PS00061. ADH_SHORT. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

ENTA_ECOLI

Accession

Primary (citable) accession number: P15047
Secondary accession number(s): P77100, Q2MBK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 16, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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