Skip Header

Contribute Send feedback
Read comments (?) or add your own

P15047 (ENTA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
EC=1.3.1.28
Gene names
Name:entA
Ordered Locus Names:b0596, JW0588
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

2,3-dihydro-2,3-dihydroxybenzoate + NAD+ = 2,3-dihydroxybenzoate + NADH.

Pathway

Siderophore biosynthesis; enterobactin biosynthesis.

Subunit structure

Homooctamer.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Sequence caution

The sequence AAB40796.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2482482,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
PRO_0000054659

Regions

Nucleotide binding9 – 3325NAD By similarity

Sites

Active site1441Proton acceptor By similarity
Binding site1311Substrate By similarity

Secondary structure

1 ................................... 248
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P15047 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: E840488335AD317B

FASTA24826,250
        10         20         30         40         50         60 
MDFSGKNVWV TGAGKGIGYA TALAFVEAGA KVTGFDQAFT QEQYPFATEV MDVADAAQVA 

        70         80         90        100        110        120 
QVCQRLLAET ERLDALVNAA GILRMGATDQ LSKEDWQQTF AVNVGGAFNL FQQTMNQFRR 

       130        140        150        160        170        180 
QRGGAIVTVA SDAAHTPRIG MSAYGASKAA LKSLALSVGL ELAGSGVRCN VVSPGSTDTD 

       190        200        210        220        230        240 
MQRTLWVSDD AEEQRIRGFG EQFKLGIPLG KIARPQEIAN TILFLASDLA SHITLQDIVV 


DGGSTLGA

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase."
Liu J., Duncan K., Walsh C.T.
J. Bacteriol. 171:791-798(1989) [PubMed: 2521622] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-14.
[2]"Nucleotide sequence and transcriptional organization of the Escherichia coli enterobactin biosynthesis cistrons entB and entA."
Nahlik M.S., Brickman T.J., Ozenberger B.A., McIntosh M.A.
J. Bacteriol. 171:784-790(1989) [PubMed: 2521621] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M24148 Unassigned DNA. Translation: AAA16103.1.
M24143 Genomic DNA. Translation: AAA76836.1.
U82598 Genomic DNA. Translation: AAB40796.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73697.1.
AP009048 Genomic DNA. Translation: BAE76351.1.
PIRDEECDB. A91904.
RefSeqNP_415128.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FWMX-ray2.00X1-248[»]
ProteinModelPortalP15047.
SMRP15047. Positions 1-248.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-9511N.
IntActP15047. 5 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000004749; EBESCP00000004749; EBESCG00000003876.
EBESCT00000014568; EBESCP00000013859; EBESCG00000013629.
GeneID945284.
GenomeReviewsGene locus JW0588 in contig AP009048_GR.
Gene locus b0596 in contig U00096_GR.
KEGGecj:JW0588.
eco:b0596.
PATRIC32116368. VBIEscCol129921_0624.

Organism-specific databases

EchoBASEEB0255.
EcoGeneEG10259. entA.

Phylogenomic databases

eggNOGCOG1028.
GeneTreeEBGT00050000009180.
HOGENOMHBG750976.
OMAQQTMAQF.
PhylomeDBP15047.
ProtClustDBPRK08220.

Enzyme and pathway databases

BioCycEcoCyc:ENTA-MONOMER.
MetaCyc:ENTA-MONOMER.

Gene expression databases

GenevestigatorP15047.

Family and domain databases

InterProIPR002198. DH_sc/Rdtase_SDR.
IPR003560. DHB_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00216.
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR01397. DHBDHDRGNASE.
PR00080. SDRFAMILY.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameENTA_ECOLI
AccessionPrimary (citable) accession number: P15047
Secondary accession number(s): P77100, Q2MBK5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: January 25, 2012
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents