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Protein

ATP-dependent DNA helicase RecQ

Gene

recQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the RecF recombination pathway; its gene expression is under the regulation of the SOS system. It is a DNA helicase.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi47 – 54ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: EcoliWiki
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' DNA helicase activity Source: GO_Central
  • ATP-dependent DNA helicase activity Source: EcoliWiki
  • DNA binding Source: EcoliWiki
  • DNA-dependent ATPase activity Source: EcoliWiki
  • four-way junction helicase activity Source: GO_Central
  • single-stranded DNA-dependent ATP-dependent DNA helicase activity Source: EcoliWiki
  • transition metal ion binding Source: EcoliWiki
  • zinc ion binding Source: EcoliWiki

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • DNA recombination Source: EcoliWiki
  • DNA repair Source: EcoliWiki
  • DNA replication Source: InterPro
  • SOS response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10833-MONOMER.
ECOL316407:JW5855-MONOMER.
MetaCyc:EG10833-MONOMER.
BRENDAi3.6.4.12. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase RecQ (EC:3.6.4.12)
Gene namesi
Name:recQ
Ordered Locus Names:b3822, JW5855
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10833. recQ.

Subcellular locationi

GO - Cellular componenti

  • bacterial nucleoid Source: UniProtKB
  • cytoplasm Source: EcoliWiki
  • replisome Source: UniProtKB
  • single-stranded DNA-dependent ATP-dependent DNA helicase complex Source: EcoliWiki
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002050332 – 609ATP-dependent DNA helicase RecQAdd BLAST608

Proteomic databases

PaxDbiP15043.
PRIDEiP15043.

Interactioni

Protein-protein interaction databases

BioGridi4259619. 148 interactors.
DIPiDIP-10656N.
IntActiP15043. 11 interactors.
MINTiMINT-1223947.
STRINGi511145.b3822.

Structurei

Secondary structure

1609
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 19Combined sources11
Helixi30 – 38Combined sources9
Beta strandi43 – 46Combined sources4
Helixi50 – 63Combined sources14
Beta strandi64 – 71Combined sources8
Helixi75 – 87Combined sources13
Beta strandi92 – 95Combined sources4
Helixi101 – 112Combined sources12
Beta strandi117 – 121Combined sources5
Helixi123 – 126Combined sources4
Beta strandi128 – 130Combined sources3
Helixi131 – 135Combined sources5
Beta strandi140 – 147Combined sources8
Helixi148 – 151Combined sources4
Helixi160 – 163Combined sources4
Helixi164 – 167Combined sources4
Helixi168 – 171Combined sources4
Beta strandi177 – 182Combined sources6
Helixi186 – 196Combined sources11
Beta strandi202 – 205Combined sources4
Beta strandi213 – 216Combined sources4
Helixi223 – 233Combined sources11
Turni234 – 236Combined sources3
Beta strandi239 – 242Combined sources4
Helixi246 – 258Combined sources13
Beta strandi263 – 266Combined sources4
Helixi272 – 283Combined sources12
Beta strandi288 – 292Combined sources5
Turni298 – 300Combined sources3
Beta strandi307 – 312Combined sources6
Helixi317 – 324Combined sources8
Beta strandi334 – 340Combined sources7
Helixi342 – 353Combined sources12
Helixi359 – 375Combined sources17
Helixi381 – 388Combined sources8
Beta strandi398 – 400Combined sources3
Helixi401 – 404Combined sources4
Beta strandi409 – 411Combined sources3
Helixi413 – 425Combined sources13
Turni426 – 428Combined sources3
Helixi432 – 440Combined sources9
Helixi445 – 449Combined sources5
Helixi452 – 454Combined sources3
Turni456 – 463Combined sources8
Helixi466 – 478Combined sources13
Beta strandi481 – 484Combined sources4
Helixi486 – 488Combined sources3
Beta strandi492 – 494Combined sources3
Helixi496 – 498Combined sources3
Helixi499 – 502Combined sources4
Beta strandi509 – 511Combined sources3
Helixi533 – 550Combined sources18
Helixi554 – 557Combined sources4
Helixi560 – 569Combined sources10
Helixi574 – 578Combined sources5
Helixi585 – 603Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OYWX-ray1.80A1-522[»]
1OYYX-ray2.50A1-523[»]
1WUDX-ray2.20A/B/D524-609[»]
ProteinModelPortaliP15043.
SMRiP15043.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15043.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 202Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST169
Domaini223 – 371Helicase C-terminalPROSITE-ProRule annotationAdd BLAST149
Domaini529 – 609HRDCPROSITE-ProRule annotationAdd BLAST81

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi146 – 149DEAH box4

Sequence similaritiesi

Belongs to the helicase family. RecQ subfamily.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 HRDC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C9U. Bacteria.
COG0514. LUCA.
HOGENOMiHOG000044388.
InParanoidiP15043.
KOiK03654.
OMAiWDATEPA.
PhylomeDBiP15043.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.80. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR004589. DNA_helicase_ATP-dep_RecQ.
IPR006293. DNA_helicase_ATP-dep_RecQ_bac.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR010997. HRDC-like.
IPR002121. HRDC_dom.
IPR027417. P-loop_NTPase.
IPR032284. RecQ_Zn-bd.
IPR018982. RQC_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00570. HRDC. 1 hit.
PF16124. RecQ_Zn_bind. 1 hit.
PF09382. RQC. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00341. HRDC. 1 hit.
SM00956. RQC. 1 hit.
[Graphical view]
SUPFAMiSSF47819. SSF47819. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR01389. recQ. 1 hit.
TIGR00614. recQ_fam. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50967. HRDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15043-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQAEVLNLE SGAKQVLQET FGYQQFRPGQ EEIIDTVLSG RDCLVVMPTG
60 70 80 90 100
GGKSLCYQIP ALLLNGLTVV VSPLISLMKD QVDQLQANGV AAACLNSTQT
110 120 130 140 150
REQQLEVMTG CRTGQIRLLY IAPERLMLDN FLEHLAHWNP VLLAVDEAHC
160 170 180 190 200
ISQWGHDFRP EYAALGQLRQ RFPTLPFMAL TATADDTTRQ DIVRLLGLND
210 220 230 240 250
PLIQISSFDR PNIRYMLMEK FKPLDQLMRY VQEQRGKSGI IYCNSRAKVE
260 270 280 290 300
DTAARLQSKG ISAAAYHAGL ENNVRADVQE KFQRDDLQIV VATVAFGMGI
310 320 330 340 350
NKPNVRFVVH FDIPRNIESY YQETGRAGRD GLPAEAMLFY DPADMAWLRR
360 370 380 390 400
CLEEKPQGQL QDIERHKLNA MGAFAEAQTC RRLVLLNYFG EGRQEPCGNC
410 420 430 440 450
DICLDPPKQY DGSTDAQIAL STIGRVNQRF GMGYVVEVIR GANNQRIRDY
460 470 480 490 500
GHDKLKVYGM GRDKSHEHWV SVIRQLIHLG LVTQNIAQHS ALQLTEAARP
510 520 530 540 550
VLRGESSLQL AVPRIVALKP KAMQKSFGGN YDRKLFAKLR KLRKSIADES
560 570 580 590 600
NVPPYVVFND ATLIEMAEQM PITASEMLSV NGVGMRKLER FGKPFMALIR

AHVDGDDEE
Length:609
Mass (Da):68,364
Last modified:January 23, 2007 - v5
Checksum:i82542DE445655D48
GO

Sequence cautioni

The sequence AAA24517 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAA67618 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti255R → A (PubMed:3027506).Curated1
Sequence conflicti255R → A (PubMed:1379743).Curated1
Sequence conflicti503 – 504RG → A (PubMed:3027506).Curated2
Sequence conflicti503 – 504RG → A (PubMed:1379743).Curated2
Sequence conflicti600R → P in AAA24517 (PubMed:3027506).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30198 Genomic DNA. Translation: AAA24517.1. Different initiation.
M87049 Genomic DNA. Translation: AAA67618.1. Different initiation.
U00096 Genomic DNA. Translation: AAT48221.1.
AP009048 Genomic DNA. Translation: BAE77479.1.
RefSeqiWP_000035581.1. NZ_LN832404.1.
YP_026263.3. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48221; AAT48221; b3822.
BAE77479; BAE77479; BAE77479.
GeneIDi948318.
KEGGiecj:JW5855.
eco:b3822.
PATRICi32123145. VBIEscCol129921_3938.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30198 Genomic DNA. Translation: AAA24517.1. Different initiation.
M87049 Genomic DNA. Translation: AAA67618.1. Different initiation.
U00096 Genomic DNA. Translation: AAT48221.1.
AP009048 Genomic DNA. Translation: BAE77479.1.
RefSeqiWP_000035581.1. NZ_LN832404.1.
YP_026263.3. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OYWX-ray1.80A1-522[»]
1OYYX-ray2.50A1-523[»]
1WUDX-ray2.20A/B/D524-609[»]
ProteinModelPortaliP15043.
SMRiP15043.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259619. 148 interactors.
DIPiDIP-10656N.
IntActiP15043. 11 interactors.
MINTiMINT-1223947.
STRINGi511145.b3822.

Proteomic databases

PaxDbiP15043.
PRIDEiP15043.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48221; AAT48221; b3822.
BAE77479; BAE77479; BAE77479.
GeneIDi948318.
KEGGiecj:JW5855.
eco:b3822.
PATRICi32123145. VBIEscCol129921_3938.

Organism-specific databases

EchoBASEiEB0826.
EcoGeneiEG10833. recQ.

Phylogenomic databases

eggNOGiENOG4105C9U. Bacteria.
COG0514. LUCA.
HOGENOMiHOG000044388.
InParanoidiP15043.
KOiK03654.
OMAiWDATEPA.
PhylomeDBiP15043.

Enzyme and pathway databases

BioCyciEcoCyc:EG10833-MONOMER.
ECOL316407:JW5855-MONOMER.
MetaCyc:EG10833-MONOMER.
BRENDAi3.6.4.12. 2026.

Miscellaneous databases

EvolutionaryTraceiP15043.
PROiP15043.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.80. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR004589. DNA_helicase_ATP-dep_RecQ.
IPR006293. DNA_helicase_ATP-dep_RecQ_bac.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR010997. HRDC-like.
IPR002121. HRDC_dom.
IPR027417. P-loop_NTPase.
IPR032284. RecQ_Zn-bd.
IPR018982. RQC_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00570. HRDC. 1 hit.
PF16124. RecQ_Zn_bind. 1 hit.
PF09382. RQC. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00341. HRDC. 1 hit.
SM00956. RQC. 1 hit.
[Graphical view]
SUPFAMiSSF47819. SSF47819. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR01389. recQ. 1 hit.
TIGR00614. recQ_fam. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50967. HRDC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRECQ_ECOLI
AccessioniPrimary (citable) accession number: P15043
Secondary accession number(s): P76762, Q2M8C7, Q6BEZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 169 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.