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Protein

ATP-dependent DNA helicase RecQ

Gene

recQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the RecF recombination pathway; its gene expression is under the regulation of the SOS system. It is a DNA helicase.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi47 – 548ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase activity Source: EcoliWiki
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' DNA helicase activity Source: GO_Central
  • ATP-dependent DNA helicase activity Source: EcoliWiki
  • DNA binding Source: EcoliWiki
  • DNA-dependent ATPase activity Source: EcoliWiki
  • four-way junction helicase activity Source: GO_Central
  • single-stranded DNA-dependent ATP-dependent DNA helicase activity Source: EcoliWiki
  • transition metal ion binding Source: EcoliWiki
  • zinc ion binding Source: EcoliWiki

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • DNA recombination Source: EcoliWiki
  • DNA repair Source: EcoliWiki
  • DNA replication Source: InterPro
  • SOS response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10833-MONOMER.
ECOL316407:JW5855-MONOMER.
MetaCyc:EG10833-MONOMER.
RETL1328306-WGS:GSTH-2991-MONOMER.
BRENDAi3.6.4.12. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase RecQ (EC:3.6.4.12)
Gene namesi
Name:recQ
Ordered Locus Names:b3822, JW5855
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10833. recQ.

Subcellular locationi

GO - Cellular componenti

  • bacterial nucleoid Source: UniProtKB
  • cytoplasm Source: EcoliWiki
  • replisome Source: UniProtKB
  • single-stranded DNA-dependent ATP-dependent DNA helicase complex Source: EcoliWiki
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 609608ATP-dependent DNA helicase RecQPRO_0000205033Add
BLAST

Proteomic databases

PaxDbiP15043.

Interactioni

Protein-protein interaction databases

BioGridi4259619. 148 interactions.
DIPiDIP-10656N.
IntActiP15043. 11 interactions.
MINTiMINT-1223947.
STRINGi511145.b3822.

Structurei

Secondary structure

1
609
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 1911Combined sources
Helixi30 – 389Combined sources
Beta strandi43 – 464Combined sources
Helixi50 – 6314Combined sources
Beta strandi64 – 718Combined sources
Helixi75 – 8713Combined sources
Beta strandi92 – 954Combined sources
Helixi101 – 11212Combined sources
Beta strandi117 – 1215Combined sources
Helixi123 – 1264Combined sources
Beta strandi128 – 1303Combined sources
Helixi131 – 1355Combined sources
Beta strandi140 – 1478Combined sources
Helixi148 – 1514Combined sources
Helixi160 – 1634Combined sources
Helixi164 – 1674Combined sources
Helixi168 – 1714Combined sources
Beta strandi177 – 1826Combined sources
Helixi186 – 19611Combined sources
Beta strandi202 – 2054Combined sources
Beta strandi213 – 2164Combined sources
Helixi223 – 23311Combined sources
Turni234 – 2363Combined sources
Beta strandi239 – 2424Combined sources
Helixi246 – 25813Combined sources
Beta strandi263 – 2664Combined sources
Helixi272 – 28312Combined sources
Beta strandi288 – 2925Combined sources
Turni298 – 3003Combined sources
Beta strandi307 – 3126Combined sources
Helixi317 – 3248Combined sources
Beta strandi334 – 3407Combined sources
Helixi342 – 35312Combined sources
Helixi359 – 37517Combined sources
Helixi381 – 3888Combined sources
Beta strandi398 – 4003Combined sources
Helixi401 – 4044Combined sources
Beta strandi409 – 4113Combined sources
Helixi413 – 42513Combined sources
Turni426 – 4283Combined sources
Helixi432 – 4409Combined sources
Helixi445 – 4495Combined sources
Helixi452 – 4543Combined sources
Turni456 – 4638Combined sources
Helixi466 – 47813Combined sources
Beta strandi481 – 4844Combined sources
Helixi486 – 4883Combined sources
Beta strandi492 – 4943Combined sources
Helixi496 – 4983Combined sources
Helixi499 – 5024Combined sources
Beta strandi509 – 5113Combined sources
Helixi533 – 55018Combined sources
Helixi554 – 5574Combined sources
Helixi560 – 56910Combined sources
Helixi574 – 5785Combined sources
Helixi585 – 60319Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OYWX-ray1.80A1-522[»]
1OYYX-ray2.50A1-523[»]
1WUDX-ray2.20A/B/D524-609[»]
ProteinModelPortaliP15043.
SMRiP15043. Positions 1-516, 530-606.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15043.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 202169Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini223 – 371149Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini529 – 60981HRDCPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi146 – 1494DEAH box

Sequence similaritiesi

Belongs to the helicase family. RecQ subfamily.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation
Contains 1 HRDC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C9U. Bacteria.
COG0514. LUCA.
HOGENOMiHOG000044388.
InParanoidiP15043.
KOiK03654.
OMAiWDATEPA.
PhylomeDBiP15043.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.80. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR004589. DNA_helicase_ATP-dep_RecQ.
IPR006293. DNA_helicase_ATP-dep_RecQ_bac.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR010997. HRDC-like.
IPR002121. HRDC_dom.
IPR027417. P-loop_NTPase.
IPR032284. RecQ_Zn-bd.
IPR018982. RQC_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00570. HRDC. 1 hit.
PF16124. RecQ_Zn_bind. 1 hit.
PF09382. RQC. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00341. HRDC. 1 hit.
SM00956. RQC. 1 hit.
[Graphical view]
SUPFAMiSSF47819. SSF47819. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR01389. recQ. 1 hit.
TIGR00614. recQ_fam. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50967. HRDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15043-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQAEVLNLE SGAKQVLQET FGYQQFRPGQ EEIIDTVLSG RDCLVVMPTG
60 70 80 90 100
GGKSLCYQIP ALLLNGLTVV VSPLISLMKD QVDQLQANGV AAACLNSTQT
110 120 130 140 150
REQQLEVMTG CRTGQIRLLY IAPERLMLDN FLEHLAHWNP VLLAVDEAHC
160 170 180 190 200
ISQWGHDFRP EYAALGQLRQ RFPTLPFMAL TATADDTTRQ DIVRLLGLND
210 220 230 240 250
PLIQISSFDR PNIRYMLMEK FKPLDQLMRY VQEQRGKSGI IYCNSRAKVE
260 270 280 290 300
DTAARLQSKG ISAAAYHAGL ENNVRADVQE KFQRDDLQIV VATVAFGMGI
310 320 330 340 350
NKPNVRFVVH FDIPRNIESY YQETGRAGRD GLPAEAMLFY DPADMAWLRR
360 370 380 390 400
CLEEKPQGQL QDIERHKLNA MGAFAEAQTC RRLVLLNYFG EGRQEPCGNC
410 420 430 440 450
DICLDPPKQY DGSTDAQIAL STIGRVNQRF GMGYVVEVIR GANNQRIRDY
460 470 480 490 500
GHDKLKVYGM GRDKSHEHWV SVIRQLIHLG LVTQNIAQHS ALQLTEAARP
510 520 530 540 550
VLRGESSLQL AVPRIVALKP KAMQKSFGGN YDRKLFAKLR KLRKSIADES
560 570 580 590 600
NVPPYVVFND ATLIEMAEQM PITASEMLSV NGVGMRKLER FGKPFMALIR

AHVDGDDEE
Length:609
Mass (Da):68,364
Last modified:January 23, 2007 - v5
Checksum:i82542DE445655D48
GO

Sequence cautioni

The sequence AAA24517 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAA67618 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti255 – 2551R → A (PubMed:3027506).Curated
Sequence conflicti255 – 2551R → A (PubMed:1379743).Curated
Sequence conflicti503 – 5042RG → A (PubMed:3027506).Curated
Sequence conflicti503 – 5042RG → A (PubMed:1379743).Curated
Sequence conflicti600 – 6001R → P in AAA24517 (PubMed:3027506).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30198 Genomic DNA. Translation: AAA24517.1. Different initiation.
M87049 Genomic DNA. Translation: AAA67618.1. Different initiation.
U00096 Genomic DNA. Translation: AAT48221.1.
AP009048 Genomic DNA. Translation: BAE77479.1.
RefSeqiWP_000035581.1. NZ_LN832404.1.
YP_026263.3. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48221; AAT48221; b3822.
BAE77479; BAE77479; BAE77479.
GeneIDi948318.
KEGGiecj:JW5855.
eco:b3822.
PATRICi32123145. VBIEscCol129921_3938.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30198 Genomic DNA. Translation: AAA24517.1. Different initiation.
M87049 Genomic DNA. Translation: AAA67618.1. Different initiation.
U00096 Genomic DNA. Translation: AAT48221.1.
AP009048 Genomic DNA. Translation: BAE77479.1.
RefSeqiWP_000035581.1. NZ_LN832404.1.
YP_026263.3. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OYWX-ray1.80A1-522[»]
1OYYX-ray2.50A1-523[»]
1WUDX-ray2.20A/B/D524-609[»]
ProteinModelPortaliP15043.
SMRiP15043. Positions 1-516, 530-606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259619. 148 interactions.
DIPiDIP-10656N.
IntActiP15043. 11 interactions.
MINTiMINT-1223947.
STRINGi511145.b3822.

Proteomic databases

PaxDbiP15043.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48221; AAT48221; b3822.
BAE77479; BAE77479; BAE77479.
GeneIDi948318.
KEGGiecj:JW5855.
eco:b3822.
PATRICi32123145. VBIEscCol129921_3938.

Organism-specific databases

EchoBASEiEB0826.
EcoGeneiEG10833. recQ.

Phylogenomic databases

eggNOGiENOG4105C9U. Bacteria.
COG0514. LUCA.
HOGENOMiHOG000044388.
InParanoidiP15043.
KOiK03654.
OMAiWDATEPA.
PhylomeDBiP15043.

Enzyme and pathway databases

BioCyciEcoCyc:EG10833-MONOMER.
ECOL316407:JW5855-MONOMER.
MetaCyc:EG10833-MONOMER.
RETL1328306-WGS:GSTH-2991-MONOMER.
BRENDAi3.6.4.12. 2026.

Miscellaneous databases

EvolutionaryTraceiP15043.
PROiP15043.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.80. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR004589. DNA_helicase_ATP-dep_RecQ.
IPR006293. DNA_helicase_ATP-dep_RecQ_bac.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR010997. HRDC-like.
IPR002121. HRDC_dom.
IPR027417. P-loop_NTPase.
IPR032284. RecQ_Zn-bd.
IPR018982. RQC_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00570. HRDC. 1 hit.
PF16124. RecQ_Zn_bind. 1 hit.
PF09382. RQC. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00341. HRDC. 1 hit.
SM00956. RQC. 1 hit.
[Graphical view]
SUPFAMiSSF47819. SSF47819. 1 hit.
SSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR01389. recQ. 1 hit.
TIGR00614. recQ_fam. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50967. HRDC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRECQ_ECOLI
AccessioniPrimary (citable) accession number: P15043
Secondary accession number(s): P76762, Q2M8C7, Q6BEZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 167 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.