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P15043 (RECQ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 141. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent DNA helicase RecQ
EC=3.6.4.12
Gene names
Name:recQ
Ordered Locus Names:b3822, JW5855
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length609 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the RecF recombination pathway; its gene expression is under the regulation of the SOS system. It is a DNA helicase. Ref.6

Catalytic activity

ATP + H2O = ADP + phosphate.

Sequence similarities

Belongs to the helicase family. RecQ subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 1 HRDC domain.

Sequence caution

The sequence AAA24517.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAA67618.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 609608ATP-dependent DNA helicase RecQ
PRO_0000205033

Regions

Domain34 – 202169Helicase ATP-binding
Domain223 – 371149Helicase C-terminal
Domain529 – 60981HRDC
Nucleotide binding47 – 548ATP Potential
Motif146 – 1494DEAH box

Experimental info

Sequence conflict2551R → A Ref.1
Sequence conflict2551R → A Ref.2
Sequence conflict503 – 5042RG → A Ref.1
Sequence conflict503 – 5042RG → A Ref.2
Sequence conflict6001R → P in AAA24517. Ref.1

Secondary structure

1 ........................................................................................................ 609
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P15043 [UniParc].

Last modified January 23, 2007. Version 5.
Checksum: 82542DE445655D48

FASTA60968,364
        10         20         30         40         50         60 
MAQAEVLNLE SGAKQVLQET FGYQQFRPGQ EEIIDTVLSG RDCLVVMPTG GGKSLCYQIP 

        70         80         90        100        110        120 
ALLLNGLTVV VSPLISLMKD QVDQLQANGV AAACLNSTQT REQQLEVMTG CRTGQIRLLY 

       130        140        150        160        170        180 
IAPERLMLDN FLEHLAHWNP VLLAVDEAHC ISQWGHDFRP EYAALGQLRQ RFPTLPFMAL 

       190        200        210        220        230        240 
TATADDTTRQ DIVRLLGLND PLIQISSFDR PNIRYMLMEK FKPLDQLMRY VQEQRGKSGI 

       250        260        270        280        290        300 
IYCNSRAKVE DTAARLQSKG ISAAAYHAGL ENNVRADVQE KFQRDDLQIV VATVAFGMGI 

       310        320        330        340        350        360 
NKPNVRFVVH FDIPRNIESY YQETGRAGRD GLPAEAMLFY DPADMAWLRR CLEEKPQGQL 

       370        380        390        400        410        420 
QDIERHKLNA MGAFAEAQTC RRLVLLNYFG EGRQEPCGNC DICLDPPKQY DGSTDAQIAL 

       430        440        450        460        470        480 
STIGRVNQRF GMGYVVEVIR GANNQRIRDY GHDKLKVYGM GRDKSHEHWV SVIRQLIHLG 

       490        500        510        520        530        540 
LVTQNIAQHS ALQLTEAARP VLRGESSLQL AVPRIVALKP KAMQKSFGGN YDRKLFAKLR 

       550        560        570        580        590        600 
KLRKSIADES NVPPYVVFND ATLIEMAEQM PITASEMLSV NGVGMRKLER FGKPFMALIR 


AHVDGDDEE

« Hide

References

« Hide 'large scale' references
[1]"The recQ gene of Escherichia coli K12: primary structure and evidence for SOS regulation."
Irino N., Nakayama K., Nakayama H.
Mol. Gen. Genet. 205:298-304(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 254.
Strain: K12 / MG1655 / ATCC 47076.
[4]"Escherichia coli K-12: a cooperatively developed annotation snapshot -- 2005."
Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R., Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T., Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H., Thomson N.R., Wishart D., Wanner B.L.
Nucleic Acids Res. 34:1-9(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 503-504.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Escherichia coli RecQ protein is a DNA helicase."
Umezu K., Nakayama K., Nakayama H.
Proc. Natl. Acad. Sci. U.S.A. 87:5363-5367(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PROTEIN SEQUENCE OF 2-6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M30198 Genomic DNA. Translation: AAA24517.1. Different initiation.
M87049 Genomic DNA. Translation: AAA67618.1. Different initiation.
U00096 Genomic DNA. Translation: AAT48221.1.
AP009048 Genomic DNA. Translation: BAE77479.1.
RefSeqYP_026263.3. NC_000913.2.
YP_491620.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1OYWX-ray1.80A4-523[»]
1OYYX-ray2.50A4-523[»]
1WUDX-ray2.20A/B/D524-608[»]
ProteinModelPortalP15043.
SMRP15043. Positions 1-516, 530-606.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-10656N.
IntActP15043. 11 interactions.
MINTMINT-1223947.
STRING511145.b3822.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAT48221; AAT48221; b3822.
BAE77479; BAE77479; BAE77479.
GeneID12930625.
948318.
KEGGecj:Y75_p3356.
eco:b3822.
PATRIC32123145. VBIEscCol129921_3938.

Organism-specific databases

EchoBASEEB0826.
EcoGeneEG10833. recQ.

Phylogenomic databases

eggNOGCOG0514.
HOGENOMHOG000044388.
KOK03654.
OMAYCLSRAK.
ProtClustDBPRK11057.

Enzyme and pathway databases

BioCycEcoCyc:EG10833-MONOMER.
ECOL316407:JW5855-MONOMER.
MetaCyc:EG10833-MONOMER.

Gene expression databases

GenevestigatorP15043.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
1.10.150.80. 1 hit.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR004589. DNA_helicase_ATP-dep_RecQ.
IPR006293. DNA_helicase_ATP-dep_RecQ_bac.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR010997. HRDC-like.
IPR002121. HRDC_dom.
IPR018982. RQC_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF00570. HRDC. 1 hit.
PF09382. RQC. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00341. HRDC. 1 hit.
SM00956. RQC. 1 hit.
[Graphical view]
SUPFAMSSF47819. HRDC_like. 1 hit.
TIGRFAMsTIGR01389. recQ. 1 hit.
TIGR00614. recQ_fam. 1 hit.
PROSITEPS00690. DEAH_ATP_HELICASE. False negative.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50967. HRDC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP15043.

Entry information

Entry nameRECQ_ECOLI
AccessionPrimary (citable) accession number: P15043
Secondary accession number(s): P76762, Q2M8C7, Q6BEZ3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: May 1, 2013
This is version 141 of the entry and version 5 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents