Reviewed, UniProtKB/Swiss-Prot P15038 (HELD_ECOLI)

Names and origin

Protein names

Recommended name:
    Helicase IV
    EC=3.6.1.-
Alternative name(s):
    75 kDa helicase

Gene names

Name:	helD
Ordered Locus Names:	b0962, JW0945

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	684 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP.
Sequence similarities	Belongs to the helicase family. UvrD subfamily. Contains 1 uvrD-like helicase ATP-binding domain.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent DNA helicase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
pepB	P37095	1	EBI-551473,EBI-549539
tufA	P0A6N1	1	EBI-551473,EBI-301077

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 684	684	Helicase IV		PRO_0000102049
Regions
	Domain	195 – 505	311	UvrD-like helicase ATP-binding
	Nucleotide binding	216 – 223	8	ATP Probable
Experimental info
	Sequence conflict	70	1	H → P in AAA23952. Ref.1
	Sequence conflict	137 – 140	4	SALP → LRVA in AAA23952. Ref.1
	Sequence conflict	365 – 366	2	MH → ID in AAA23952. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P15038-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: FC1A6250537A4A24	FASTA	684	77,976
	10 20 30 40 50 60 MELKATTLGK RLAQHPYDRA VILNAGIKVS GDRHEYLIPF NQLLAIHCKR GLVWGELEFV 70 80 90 100 110 120 LPDEKVVRLH GTEWGETQRF YHHLDAHWRR WSGEMSEIAS GVLRQQLDLI ATRTGENKWL 130 140 150 160 170 180 TREQTSGVQQ QIRQALSALP LPVNRLEEFD NCREAWRKCQ AWLKDIESAR LQHNQAYTEA 190 200 210 220 230 240 MLTEYADFFR QVESSPLNPA QARAVVNGEH SLLVLAGAGS GKTSVLVARA GWLLARGEAS 250 260 270 280 290 300 PEQILLLAFG RKAAEEMDER IRERLHTEDI TARTFHALAL HIIQQGSKKV PIVSKLENDT 310 320 330 340 350 360 AARHELFIAE WRKQCSEKKA QAKGWRQWLT EEMQWSVPEG NFWDDEKLQR RLASRLDRWV 370 380 390 400 410 420 SLMRMHGGAQ AEMIASAPEE IRDLFSKRIK LMAPLLKAWK GALKAENAVD FSGLIHQAIV 430 440 450 460 470 480 ILEKGRFISP WKHILVDEFQ DISPQRAALL AALRKQNSQT TLFAVGDDWQ AIYRFSGAQM 490 500 510 520 530 540 SLTTAFHENF GEGERCDLDT TYRFNSRIGE VANRFIQQNP GQLKKPLNSL TNGDKKAVTL 550 560 570 580 590 600 LDESQLDALL DKLSGYAKPE ERILILARYH HMRPASLEKA ATRWPKLQID FMTIHASKGQ 610 620 630 640 650 660 QADYVIIVGL QEGSDGFPAA ARESIMEEAL LPPVEDFPDA EERRLMYVAL TRARHRVWAL 670 680 FNKENPSPFV EILKNLDVPV ARKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The molecular cloning of the gene encoding the Escherichia coli 75-kDa helicase and the determination of its nucleotide sequence and gentic map position." Wood E.R., Matson S.W. J. Biol. Chem. 264:8297-8303(1989) [PubMed: 2542273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-27.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J04726 Genomic DNA. Translation: AAA23952.1. U00096 Genomic DNA. Translation: AAC74048.1. AP009048 Genomic DNA. Translation: BAA35727.1.
PIR	HJECD4. A64837.
RefSeq	AP_001592.1. NP_415482.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9876N.
IntAct	P15038. 33 interactions.
STRING	P15038.
Genome annotation databases
GeneID	946227.
GenomeReviews	Gene locus JW0945 in contig AP009048_GR. Gene locus b0962 in contig U00096_GR.
KEGG	ecj:JW0945. eco:b0962.
Organism-specific databases
EchoBASE	EB0421.
EcoGene	EG10426. helD.
CMR	Search...
Phylogenomic databases
HOGENOM	P15038.
OMA	VSPWKHI
Enzyme and pathway databases
BioCyc	EcoCyc:EG10426-MON. ECOL168927:B0962-MON.
Gene expression databases
Genevestigator	P15038.
Family and domain databases
InterPro	IPR000212. DNA_helicase_UvrD/REP. IPR014016. Helicase_SF1_UvrD-rel. IPR003661. Sig_transdc_His_kin_sub1_dim/P. [Graphical view]
PANTHER	PTHR11070. UvrD_helicase. 1 hit.
Pfam	PF00580. UvrD-helicase. 2 hits. [Graphical view]
SMART	SM00388. HisKA. 1 hit. [Graphical view]
PROSITE	PS51198. UVRD_HELICASE_ATP_BIND. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HELD_ECOLI

Accession

Primary (citable) accession number: P15038
Secondary accession number(s): P77623

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	November 1, 1997
Last modified:	November 24, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)