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Protein

Helicase IV

Gene

helD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei503 – 5031ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi216 – 2238ATPCurated

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent 3'-5' DNA helicase activity Source: EcoCyc
  • DNA binding Source: UniProtKB-KW

GO - Biological processi

  • DNA recombination Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10426-MONOMER.
ECOL316407:JW0945-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Helicase IV (EC:3.6.4.12)
Alternative name(s):
75 kDa helicase
Gene namesi
Name:helD
Ordered Locus Names:b0962, JW0945
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10426. helD.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • DNA helicase complex Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4405.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 684684Helicase IVPRO_0000102049Add
BLAST

Proteomic databases

PaxDbiP15038.
PRIDEiP15038.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
cyaAP009363EBI-551473,EBI-1116685
tufAP0CE472EBI-551473,EBI-301077

Protein-protein interaction databases

BioGridi4263362. 127 interactions.
DIPiDIP-9876N.
IntActiP15038. 34 interactions.
MINTiMINT-1226516.
STRINGi511145.b0962.

Chemistry

BindingDBiP15038.

Structurei

3D structure databases

ProteinModelPortaliP15038.
SMRiP15038. Positions 187-527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini195 – 505311UvrD-like helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. UvrD subfamily.Curated
Contains 1 uvrD-like helicase ATP-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41088T4. Bacteria.
COG0210. LUCA.
HOGENOMiHOG000118816.
InParanoidiP15038.
KOiK03658.
OMAiHYFGEGD.
OrthoDBiEOG6HJ254.
PhylomeDBiP15038.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR022161. Helicase_IV_N.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 1 hit.
PfamiPF12462. Helicase_IV_N. 1 hit.
PF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15038-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELKATTLGK RLAQHPYDRA VILNAGIKVS GDRHEYLIPF NQLLAIHCKR
60 70 80 90 100
GLVWGELEFV LPDEKVVRLH GTEWGETQRF YHHLDAHWRR WSGEMSEIAS
110 120 130 140 150
GVLRQQLDLI ATRTGENKWL TREQTSGVQQ QIRQALSALP LPVNRLEEFD
160 170 180 190 200
NCREAWRKCQ AWLKDIESAR LQHNQAYTEA MLTEYADFFR QVESSPLNPA
210 220 230 240 250
QARAVVNGEH SLLVLAGAGS GKTSVLVARA GWLLARGEAS PEQILLLAFG
260 270 280 290 300
RKAAEEMDER IRERLHTEDI TARTFHALAL HIIQQGSKKV PIVSKLENDT
310 320 330 340 350
AARHELFIAE WRKQCSEKKA QAKGWRQWLT EEMQWSVPEG NFWDDEKLQR
360 370 380 390 400
RLASRLDRWV SLMRMHGGAQ AEMIASAPEE IRDLFSKRIK LMAPLLKAWK
410 420 430 440 450
GALKAENAVD FSGLIHQAIV ILEKGRFISP WKHILVDEFQ DISPQRAALL
460 470 480 490 500
AALRKQNSQT TLFAVGDDWQ AIYRFSGAQM SLTTAFHENF GEGERCDLDT
510 520 530 540 550
TYRFNSRIGE VANRFIQQNP GQLKKPLNSL TNGDKKAVTL LDESQLDALL
560 570 580 590 600
DKLSGYAKPE ERILILARYH HMRPASLEKA ATRWPKLQID FMTIHASKGQ
610 620 630 640 650
QADYVIIVGL QEGSDGFPAA ARESIMEEAL LPPVEDFPDA EERRLMYVAL
660 670 680
TRARHRVWAL FNKENPSPFV EILKNLDVPV ARKP
Length:684
Mass (Da):77,976
Last modified:November 1, 1997 - v2
Checksum:iFC1A6250537A4A24
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701H → P in AAA23952 (PubMed:2542273).Curated
Sequence conflicti137 – 1404SALP → LRVA in AAA23952 (PubMed:2542273).Curated
Sequence conflicti365 – 3662MH → ID in AAA23952 (PubMed:2542273).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04726 Genomic DNA. Translation: AAA23952.1.
U00096 Genomic DNA. Translation: AAC74048.1.
AP009048 Genomic DNA. Translation: BAA35727.1.
PIRiA64837. HJECD4.
RefSeqiNP_415482.1. NC_000913.3.
WP_001295354.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74048; AAC74048; b0962.
BAA35727; BAA35727; BAA35727.
GeneIDi946227.
KEGGiecj:JW0945.
eco:b0962.
PATRICi32117143. VBIEscCol129921_0996.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04726 Genomic DNA. Translation: AAA23952.1.
U00096 Genomic DNA. Translation: AAC74048.1.
AP009048 Genomic DNA. Translation: BAA35727.1.
PIRiA64837. HJECD4.
RefSeqiNP_415482.1. NC_000913.3.
WP_001295354.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP15038.
SMRiP15038. Positions 187-527.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263362. 127 interactions.
DIPiDIP-9876N.
IntActiP15038. 34 interactions.
MINTiMINT-1226516.
STRINGi511145.b0962.

Chemistry

BindingDBiP15038.
ChEMBLiCHEMBL4405.

Proteomic databases

PaxDbiP15038.
PRIDEiP15038.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74048; AAC74048; b0962.
BAA35727; BAA35727; BAA35727.
GeneIDi946227.
KEGGiecj:JW0945.
eco:b0962.
PATRICi32117143. VBIEscCol129921_0996.

Organism-specific databases

EchoBASEiEB0421.
EcoGeneiEG10426. helD.

Phylogenomic databases

eggNOGiENOG41088T4. Bacteria.
COG0210. LUCA.
HOGENOMiHOG000118816.
InParanoidiP15038.
KOiK03658.
OMAiHYFGEGD.
OrthoDBiEOG6HJ254.
PhylomeDBiP15038.

Enzyme and pathway databases

BioCyciEcoCyc:EG10426-MONOMER.
ECOL316407:JW0945-MONOMER.

Miscellaneous databases

PROiP15038.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR022161. Helicase_IV_N.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 1 hit.
PfamiPF12462. Helicase_IV_N. 1 hit.
PF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The molecular cloning of the gene encoding the Escherichia coli 75-kDa helicase and the determination of its nucleotide sequence and gentic map position."
    Wood E.R., Matson S.W.
    J. Biol. Chem. 264:8297-8303(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-27.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiHELD_ECOLI
AccessioniPrimary (citable) accession number: P15038
Secondary accession number(s): P77623
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1997
Last modified: July 6, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.