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Protein

Xaa-Pro aminopeptidase

Gene

pepP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.

Cofactori

Mn2+Note: Binds 2 manganese ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi261Manganese 21
Metal bindingi272Manganese 11
Metal bindingi272Manganese 21
Metal bindingi355Manganese 11
Metal bindingi384Manganese 11
Metal bindingi407Manganese 11
Metal bindingi407Manganese 21

GO - Molecular functioni

  • aminopeptidase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • manganese ion binding Source: EcoCyc
  • metalloexopeptidase activity Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10697-MONOMER.
ECOL316407:JW2876-MONOMER.
MetaCyc:EG10697-MONOMER.
BRENDAi3.1.8.1. 2026.
3.4.11.9. 2026.

Protein family/group databases

MEROPSiM24.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Xaa-Pro aminopeptidase (EC:3.4.11.9)
Alternative name(s):
Aminoacylproline aminopeptidase
Aminopeptidase P II
Short name:
APP-II
X-Pro aminopeptidase
Gene namesi
Name:pepP
Ordered Locus Names:b2908, JW2876
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10697. pepP.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001850752 – 441Xaa-Pro aminopeptidaseAdd BLAST440

Proteomic databases

EPDiP15034.
PaxDbiP15034.
PRIDEiP15034.

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
guaCP605602EBI-554801,EBI-544491

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4259236. 11 interactors.
DIPiDIP-10459N.
IntActiP15034. 10 interactors.
MINTiMINT-1252312.
STRINGi511145.b2908.

Structurei

Secondary structure

1441
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 19Combined sources14
Beta strandi22 – 29Combined sources8
Beta strandi35 – 37Combined sources3
Helixi48 – 54Combined sources7
Beta strandi62 – 67Combined sources6
Beta strandi69 – 71Combined sources3
Beta strandi73 – 79Combined sources7
Helixi84 – 90Combined sources7
Helixi95 – 103Combined sources9
Beta strandi106 – 110Combined sources5
Helixi111 – 113Combined sources3
Helixi114 – 122Combined sources9
Beta strandi126 – 129Combined sources4
Helixi136 – 150Combined sources15
Helixi153 – 155Combined sources3
Beta strandi161 – 164Combined sources4
Helixi167 – 175Combined sources9
Helixi179 – 202Combined sources24
Helixi209 – 222Combined sources14
Beta strandi227 – 230Combined sources4
Beta strandi233 – 236Combined sources4
Helixi237 – 241Combined sources5
Beta strandi257 – 262Combined sources6
Beta strandi264 – 266Combined sources3
Beta strandi273 – 278Combined sources6
Helixi285 – 304Combined sources20
Helixi311 – 328Combined sources18
Helixi336 – 341Combined sources6
Turni342 – 348Combined sources7
Beta strandi358 – 362Combined sources5
Helixi369 – 371Combined sources3
Beta strandi380 – 383Combined sources4
Beta strandi386 – 389Combined sources4
Helixi397 – 399Combined sources3
Beta strandi402 – 405Combined sources4
Beta strandi407 – 413Combined sources7
Beta strandi416 – 421Combined sources6
Helixi428 – 439Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A16X-ray2.30A2-441[»]
1JAWX-ray2.70A2-441[»]
1M35X-ray2.40A/B/C/D/E/F2-441[»]
1N51X-ray2.30A2-441[»]
1W2MX-ray2.40A/B/C/D/E/F2-441[»]
1W7VX-ray2.00A/B/C/D2-441[»]
1WBQX-ray2.30A/B/C/D2-441[»]
1WL6X-ray2.00A2-441[»]
1WL9X-ray1.90A2-441[»]
1WLRX-ray2.10A2-441[»]
2BH3X-ray2.40A2-441[»]
2BHAX-ray2.40A2-441[»]
2BHBX-ray2.41A2-441[»]
2BHCX-ray2.40A2-441[»]
2BHDX-ray2.50A2-441[»]
2BN7X-ray2.40A2-441[»]
2BWSX-ray1.75A2-441[»]
2BWTX-ray2.90A2-441[»]
2BWUX-ray2.20A2-441[»]
2BWVX-ray1.70A2-441[»]
2BWWX-ray2.61A2-441[»]
2BWXX-ray1.70A2-441[»]
2BWYX-ray2.40A2-441[»]
2V3XX-ray1.70A2-441[»]
2V3YX-ray1.60A2-441[»]
2V3ZX-ray1.56A2-441[»]
ProteinModelPortaliP15034.
SMRiP15034.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15034.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24B family.Curated

Phylogenomic databases

eggNOGiENOG4107RAQ. Bacteria.
COG0006. LUCA.
HOGENOMiHOG000008762.
InParanoidiP15034.
KOiK01262.
OMAiKDPLMET.
PhylomeDBiP15034.

Family and domain databases

Gene3Di3.40.350.10. 1 hit.
3.90.230.10. 1 hit.
InterProiIPR007865. Aminopep_P_N.
IPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR001131. Peptidase_M24B_aminopep-P_CS.
[Graphical view]
PfamiPF05195. AMP_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SMARTiSM01011. AMP_N. 1 hit.
[Graphical view]
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiPS00491. PROLINE_PEPTIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15034-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEISRQEFQ RRRQALVEQM QPGSAALIFA APEVTRSADS EYPYRQNSDF
60 70 80 90 100
WYFTGFNEPE AVLVLIKSDD THNHSVLFNR VRDLTAEIWF GRRLGQDAAP
110 120 130 140 150
EKLGVDRALA FSEINQQLYQ LLNGLDVVYH AQGEYAYADV IVNSALEKLR
160 170 180 190 200
KGSRQNLTAP ATMIDWRPVV HEMRLFKSPE EIAVLRRAGE ITAMAHTRAM
210 220 230 240 250
EKCRPGMFEY HLEGEIHHEF NRHGARYPSY NTIVGSGENG CILHYTENEC
260 270 280 290 300
EMRDGDLVLI DAGCEYKGYA GDITRTFPVN GKFTQAQREI YDIVLESLET
310 320 330 340 350
SLRLYRPGTS ILEVTGEVVR IMVSGLVKLG ILKGDVDELI AQNAHRPFFM
360 370 380 390 400
HGLSHWLGLD VHDVGVYGQD RSRILEPGMV LTVEPGLYIA PDAEVPEQYR
410 420 430 440
GIGIRIEDDI VITETGNENL TASVVKKPEE IEALMVAARK Q
Length:441
Mass (Da):49,815
Last modified:January 23, 2007 - v2
Checksum:i80A6A5BDD86D84B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00398 Genomic DNA. Translation: BAA00299.1.
D90281 Genomic DNA. Translation: BAA14325.1.
U28377 Genomic DNA. Translation: AAA69076.1.
U00096 Genomic DNA. Translation: AAC75946.1.
AP009048 Genomic DNA. Translation: BAE76973.1.
PIRiJX0067. DPECP.
RefSeqiNP_417384.1. NC_000913.3.
WP_001290136.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75946; AAC75946; b2908.
BAE76973; BAE76973; BAE76973.
GeneIDi947385.
KEGGiecj:JW2876.
eco:b2908.
PATRICi32121230. VBIEscCol129921_3003.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00398 Genomic DNA. Translation: BAA00299.1.
D90281 Genomic DNA. Translation: BAA14325.1.
U28377 Genomic DNA. Translation: AAA69076.1.
U00096 Genomic DNA. Translation: AAC75946.1.
AP009048 Genomic DNA. Translation: BAE76973.1.
PIRiJX0067. DPECP.
RefSeqiNP_417384.1. NC_000913.3.
WP_001290136.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A16X-ray2.30A2-441[»]
1JAWX-ray2.70A2-441[»]
1M35X-ray2.40A/B/C/D/E/F2-441[»]
1N51X-ray2.30A2-441[»]
1W2MX-ray2.40A/B/C/D/E/F2-441[»]
1W7VX-ray2.00A/B/C/D2-441[»]
1WBQX-ray2.30A/B/C/D2-441[»]
1WL6X-ray2.00A2-441[»]
1WL9X-ray1.90A2-441[»]
1WLRX-ray2.10A2-441[»]
2BH3X-ray2.40A2-441[»]
2BHAX-ray2.40A2-441[»]
2BHBX-ray2.41A2-441[»]
2BHCX-ray2.40A2-441[»]
2BHDX-ray2.50A2-441[»]
2BN7X-ray2.40A2-441[»]
2BWSX-ray1.75A2-441[»]
2BWTX-ray2.90A2-441[»]
2BWUX-ray2.20A2-441[»]
2BWVX-ray1.70A2-441[»]
2BWWX-ray2.61A2-441[»]
2BWXX-ray1.70A2-441[»]
2BWYX-ray2.40A2-441[»]
2V3XX-ray1.70A2-441[»]
2V3YX-ray1.60A2-441[»]
2V3ZX-ray1.56A2-441[»]
ProteinModelPortaliP15034.
SMRiP15034.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259236. 11 interactors.
DIPiDIP-10459N.
IntActiP15034. 10 interactors.
MINTiMINT-1252312.
STRINGi511145.b2908.

Protein family/group databases

MEROPSiM24.004.

Proteomic databases

EPDiP15034.
PaxDbiP15034.
PRIDEiP15034.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75946; AAC75946; b2908.
BAE76973; BAE76973; BAE76973.
GeneIDi947385.
KEGGiecj:JW2876.
eco:b2908.
PATRICi32121230. VBIEscCol129921_3003.

Organism-specific databases

EchoBASEiEB0691.
EcoGeneiEG10697. pepP.

Phylogenomic databases

eggNOGiENOG4107RAQ. Bacteria.
COG0006. LUCA.
HOGENOMiHOG000008762.
InParanoidiP15034.
KOiK01262.
OMAiKDPLMET.
PhylomeDBiP15034.

Enzyme and pathway databases

BioCyciEcoCyc:EG10697-MONOMER.
ECOL316407:JW2876-MONOMER.
MetaCyc:EG10697-MONOMER.
BRENDAi3.1.8.1. 2026.
3.4.11.9. 2026.

Miscellaneous databases

EvolutionaryTraceiP15034.
PROiP15034.

Family and domain databases

Gene3Di3.40.350.10. 1 hit.
3.90.230.10. 1 hit.
InterProiIPR007865. Aminopep_P_N.
IPR029149. Creatin/AminoP/Spt16_NTD.
IPR028980. Creatinase/Aminopeptidase_P_N.
IPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR001131. Peptidase_M24B_aminopep-P_CS.
[Graphical view]
PfamiPF05195. AMP_N. 1 hit.
PF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SMARTiSM01011. AMP_N. 1 hit.
[Graphical view]
SUPFAMiSSF53092. SSF53092. 1 hit.
SSF55920. SSF55920. 1 hit.
PROSITEiPS00491. PROLINE_PEPTIDASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPP_ECOLI
AccessioniPrimary (citable) accession number: P15034
Secondary accession number(s): Q2M9T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 163 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.