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Protein

Exodeoxyribonuclease 8

Gene

recE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Is involved in the RecE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA.

Catalytic activityi

Degradation of double-stranded DNA. It acts progressively in a 5' to 3' direction, releasing 5'-phosphomononucleotides.

GO - Molecular functioni

  • double-stranded DNA 5'-3' exodeoxyribonuclease activity Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BioCyciEcoCyc:EG10827-MONOMER.
ECOL316407:JW1344-MONOMER.
MetaCyc:EG10827-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Exodeoxyribonuclease 8 (EC:3.1.11.-)
Alternative name(s):
Exodeoxyribonuclease VIII
Short name:
EXO VIII
Gene namesi
Name:recE
Ordered Locus Names:b1350, JW1344
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10827. recE.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000972271 – 866Exodeoxyribonuclease 8Add BLAST866

Proteomic databases

PaxDbiP15032.
PRIDEiP15032.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi4260163. 103 interactors.
DIPiDIP-10652N.
IntActiP15032. 11 interactors.
MINTiMINT-1262359.
STRINGi511145.b1350.

Structurei

Secondary structure

1866
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi616 – 624Combined sources9
Helixi626 – 632Combined sources7
Helixi647 – 655Combined sources9
Helixi658 – 661Combined sources4
Helixi704 – 712Combined sources9
Helixi716 – 722Combined sources7
Beta strandi723 – 725Combined sources3
Beta strandi727 – 729Combined sources3
Beta strandi732 – 735Combined sources4
Turni737 – 739Combined sources3
Beta strandi742 – 745Combined sources4
Beta strandi748 – 751Combined sources4
Helixi752 – 754Combined sources3
Beta strandi756 – 764Combined sources9
Helixi766 – 772Combined sources7
Helixi773 – 775Combined sources3
Turni776 – 779Combined sources4
Helixi780 – 793Combined sources14
Beta strandi798 – 807Combined sources10
Beta strandi815 – 819Combined sources5
Helixi822 – 844Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3H4RX-ray2.80A606-866[»]
ProteinModelPortaliP15032.
SMRiP15032.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP15032.

Family & Domainsi

Phylogenomic databases

eggNOGiENOG41076J9. Bacteria.
ENOG410XPW2. LUCA.
HOGENOMiHOG000120165.
KOiK10906.
OMAiWKFTSAN.

Family and domain databases

InterProiIPR010584. ExoDNase_VIII.
IPR024432. Put_RecE_PDDEXK-like_dom.
[Graphical view]
PfamiPF12684. DUF3799. 1 hit.
PF06630. Exonuc_VIII. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P15032-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTKPLFLLR KAKKSSGEPD VVLWASNDFE STCATLDYLI VKSGKKLSSY
60 70 80 90 100
FKAVATNFPV VNDLPAEGEI DFTWSERYQL SKDSMTWELK PGAAPDNAHY
110 120 130 140 150
QGNTNVNGED MTEIEENMLL PISGQELPIR WLAQHGSEKP VTHVSRDGLQ
160 170 180 190 200
ALHIARAEEL PAVTALAVSH KTSLLDPLEI RELHKLVRDT DKVFPNPGNS
210 220 230 240 250
NLGLITAFFE AYLNADYTDR GLLTKEWMKG NRVSHITRTA SGANAGGGNL
260 270 280 290 300
TDRGEGFVHD LTSLARDVAT GVLARSMDLD IYNLHPAHAK RIEEIIAENK
310 320 330 340 350
PPFSVFRDKF ITMPGGLDYS RAIVVASVKE APIGIEVIPA HVTEYLNKVL
360 370 380 390 400
TETDHANPDP EIVDIACGRS SAPMPQRVTE EGKQDDEEKP QPSGTTAVEQ
410 420 430 440 450
GEAETMEPDA TEHHQDTQPL DAQSQVNSVD AKYQELRAEL HEARKNIPSK
460 470 480 490 500
NPVDDDKLLA ASRGEFVDGI SDPNDPKWVK GIQTRDCVYQ NQPETEKTSP
510 520 530 540 550
DMNQPEPVVQ QEPEIACNAC GQTGGDNCPD CGAVMGDATY QETFDEESQV
560 570 580 590 600
EAKENDPEEM EGAEHPHNEN AGSDPHRDCS DETGEVADPV IVEDIEPGIY
610 620 630 640 650
YGISNENYHA GPGISKSQLD DIADTPALYL WRKNAPVDTT KTKTLDLGTA
660 670 680 690 700
FHCRVLEPEE FSNRFIVAPE FNRRTNAGKE EEKAFLMECA STGKTVITAE
710 720 730 740 750
EGRKIELMYQ SVMALPLGQW LVESAGHAES SIYWEDPETG ILCRCRPDKI
760 770 780 790 800
IPEFHWIMDV KTTADIQRFK TAYYDYRYHV QDAFYSDGYE AQFGVQPTFV
810 820 830 840 850
FLVASTTIEC GRYPVEIFMM GEEAKLAGQQ EYHRNLRTLS DCLNTDEWPA
860
IKTLSLPRWA KEYAND
Length:866
Mass (Da):96,368
Last modified:November 1, 1997 - v3
Checksum:i6133E2FDDB12A538
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti366A → G in AAA03212 (PubMed:2649487).Curated1
Sequence conflicti691 – 692ST → TA (PubMed:8244937).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74432.1.
AP009048 Genomic DNA. Translation: BAA14950.1.
M24905 Unassigned DNA. Translation: AAA03212.1.
X55956 Genomic DNA. Translation: CAA39419.1. Sequence problems.
X55956 Genomic DNA. Translation: CAA39420.1. Sequence problems.
X55956 Genomic DNA. Translation: CAA39421.1. Sequence problems.
X55956 Genomic DNA. Translation: CAA39422.1. Sequence problems.
X55956 Genomic DNA. Translation: CAA39423.1. Sequence problems.
L23927 Unassigned DNA. Translation: AAA16178.1.
PIRiA64885. NCECX8.
RefSeqiNP_415866.1. NC_000913.3.
WP_000105143.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC74432; AAC74432; b1350.
BAA14950; BAA14950; BAA14950.
GeneIDi945918.
KEGGiecj:JW1344.
eco:b1350.
PATRICi32117978. VBIEscCol129921_1409.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74432.1.
AP009048 Genomic DNA. Translation: BAA14950.1.
M24905 Unassigned DNA. Translation: AAA03212.1.
X55956 Genomic DNA. Translation: CAA39419.1. Sequence problems.
X55956 Genomic DNA. Translation: CAA39420.1. Sequence problems.
X55956 Genomic DNA. Translation: CAA39421.1. Sequence problems.
X55956 Genomic DNA. Translation: CAA39422.1. Sequence problems.
X55956 Genomic DNA. Translation: CAA39423.1. Sequence problems.
L23927 Unassigned DNA. Translation: AAA16178.1.
PIRiA64885. NCECX8.
RefSeqiNP_415866.1. NC_000913.3.
WP_000105143.1. NZ_CP014272.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3H4RX-ray2.80A606-866[»]
ProteinModelPortaliP15032.
SMRiP15032.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260163. 103 interactors.
DIPiDIP-10652N.
IntActiP15032. 11 interactors.
MINTiMINT-1262359.
STRINGi511145.b1350.

Proteomic databases

PaxDbiP15032.
PRIDEiP15032.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74432; AAC74432; b1350.
BAA14950; BAA14950; BAA14950.
GeneIDi945918.
KEGGiecj:JW1344.
eco:b1350.
PATRICi32117978. VBIEscCol129921_1409.

Organism-specific databases

EchoBASEiEB0820.
EcoGeneiEG10827. recE.

Phylogenomic databases

eggNOGiENOG41076J9. Bacteria.
ENOG410XPW2. LUCA.
HOGENOMiHOG000120165.
KOiK10906.
OMAiWKFTSAN.

Enzyme and pathway databases

BioCyciEcoCyc:EG10827-MONOMER.
ECOL316407:JW1344-MONOMER.
MetaCyc:EG10827-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP15032.
PROiP15032.

Family and domain databases

InterProiIPR010584. ExoDNase_VIII.
IPR024432. Put_RecE_PDDEXK-like_dom.
[Graphical view]
PfamiPF12684. DUF3799. 1 hit.
PF06630. Exonuc_VIII. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRECE_ECOLI
AccessioniPrimary (citable) accession number: P15032
Secondary accession number(s): P76848, P77807
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.