Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Fe(3+) dicitrate-binding periplasmic protein

Gene

fecB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Binds citrate-dependent Fe3+; part of the binding-protein-dependent transport system for uptake of citrate-dependent Fe3+.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciEcoCyc:FECB-MONOMER.
ECOL316407:JW4250-MONOMER.
MetaCyc:FECB-MONOMER.

Protein family/group databases

TCDBi3.A.1.14.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Fe(3+) dicitrate-binding periplasmic protein
Alternative name(s):
Iron(III) dicitrate-binding periplasmic protein
Gene namesi
Name:fecB
Ordered Locus Names:b4290, JW4250
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10287. fecB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 300279Fe(3+) dicitrate-binding periplasmic proteinPRO_0000031820Add
BLAST

Proteomic databases

PaxDbiP15028.

Expressioni

Inductioni

Induced 1.8-fold by hydroxyurea.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261562. 211 interactions.
DIPiDIP-9584N.
IntActiP15028. 10 interactions.
MINTiMINT-1275090.
STRINGi511145.b4290.

Structurei

3D structure databases

ProteinModelPortaliP15028.
SMRiP15028. Positions 19-290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 295257Fe/B12 periplasmic-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Fe/B12 periplasmic-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108HUF. Bacteria.
COG4594. LUCA.
HOGENOMiHOG000045521.
InParanoidiP15028.
KOiK02016.
OMAiQPAYLEN.
OrthoDBiEOG6J1DGT.
PhylomeDBiP15028.

Family and domain databases

InterProiIPR002491. ABC_transptr_periplasmic_BD.
[Graphical view]
PfamiPF01497. Peripla_BP_2. 1 hit.
[Graphical view]
PROSITEiPS50983. FE_B12_PBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P15028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAFIRFLFA GLLLVISHAF AATVQDEHGT FTLEKTPQRI VVLELSFADA
60 70 80 90 100
LAAVDVIPIG IADDNDAKRI LPEVRAHLKP WQSVGTRAQP SLEAIAALKP
110 120 130 140 150
DLIIADSSRH AGVYIALQQI APVLLLKSRN ETYAENLQSA AIIGEMVGKK
160 170 180 190 200
REMQARLEQH KERMAQWASQ LPKGTRVAFG TSREQQFNLH TQETWTGSVL
210 220 230 240 250
ASLGLNVPAA MAGASMPSIG LEQLLAVNPA WLLVAHYREE SIVKRWQQDP
260 270 280 290 300
LWQMLTAAQK QQVASVDSNT WARMRGIFAA ERIAADTVKI FHHQPLTVVK
Length:300
Mass (Da):33,146
Last modified:July 19, 2003 - v3
Checksum:i6024ED0F9C82D0EA
GO

Sequence cautioni

The sequence AAA97186.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231T → M in AAA23762 (PubMed:2651410).Curated
Sequence conflicti57 – 571I → S in AAA23762 (PubMed:2651410).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti8 – 81L → V in strain: B.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26397 Genomic DNA. Translation: AAA23762.1.
U14003 Genomic DNA. Translation: AAA97186.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77246.2.
AP009048 Genomic DNA. Translation: BAE78281.1.
PIRiS56515. QRECD3.
RefSeqiNP_418710.4. NC_000913.3.
WP_000879155.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77246; AAC77246; b4290.
BAE78281; BAE78281; BAE78281.
GeneIDi946838.
KEGGiecj:JW4250.
eco:b4290.
PATRICi32124159. VBIEscCol129921_4423.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26397 Genomic DNA. Translation: AAA23762.1.
U14003 Genomic DNA. Translation: AAA97186.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77246.2.
AP009048 Genomic DNA. Translation: BAE78281.1.
PIRiS56515. QRECD3.
RefSeqiNP_418710.4. NC_000913.3.
WP_000879155.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP15028.
SMRiP15028. Positions 19-290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261562. 211 interactions.
DIPiDIP-9584N.
IntActiP15028. 10 interactions.
MINTiMINT-1275090.
STRINGi511145.b4290.

Protein family/group databases

TCDBi3.A.1.14.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP15028.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77246; AAC77246; b4290.
BAE78281; BAE78281; BAE78281.
GeneIDi946838.
KEGGiecj:JW4250.
eco:b4290.
PATRICi32124159. VBIEscCol129921_4423.

Organism-specific databases

EchoBASEiEB0283.
EcoGeneiEG10287. fecB.

Phylogenomic databases

eggNOGiENOG4108HUF. Bacteria.
COG4594. LUCA.
HOGENOMiHOG000045521.
InParanoidiP15028.
KOiK02016.
OMAiQPAYLEN.
OrthoDBiEOG6J1DGT.
PhylomeDBiP15028.

Enzyme and pathway databases

BioCyciEcoCyc:FECB-MONOMER.
ECOL316407:JW4250-MONOMER.
MetaCyc:FECB-MONOMER.

Miscellaneous databases

PROiP15028.

Family and domain databases

InterProiIPR002491. ABC_transptr_periplasmic_BD.
[Graphical view]
PfamiPF01497. Peripla_BP_2. 1 hit.
[Graphical view]
PROSITEiPS50983. FE_B12_PBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic-binding-protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli."
    Staudenmaier H., van Hove B., Yaraghi Z., Braun V.
    J. Bacteriol. 171:2626-2633(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Hydroxyurea induces hydroxyl radical-mediated cell death in Escherichia coli."
    Davies B.W., Kohanski M.A., Simmons L.A., Winkler J.A., Collins J.J., Walker G.C.
    Mol. Cell 36:845-860(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY HYDROXYUREA.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Entry informationi

Entry nameiFECB_ECOLI
AccessioniPrimary (citable) accession number: P15028
Secondary accession number(s): P76816, Q2M625
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: July 19, 2003
Last modified: January 20, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.