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Protein

Enolase

Gene

Eno

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+Note: Mg2+ is required for catalysis and for stabilizing the dimer.

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (Gapdh1), Glyceraldehyde-3-phosphate dehydrogenase 2 (Gapdh2), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh2), Glyceraldehyde-3-phosphate dehydrogenase (CG9010)
  2. Phosphoglycerate kinase (Pgk)
  3. no protein annotated in this organism
  4. Enolase (Eno)
  5. Pyruvate kinase (CG7069), Pyruvate kinase (CG7069), Pyruvate kinase (CG7362), Pyruvate kinase (PyK), Pyruvate kinase (CG2964), Pyruvate kinase (CG2964)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225SubstrateBy similarity1
Binding sitei234SubstrateBy similarity1
Active sitei277Proton donorBy similarity1
Metal bindingi312MagnesiumBy similarity1
Metal bindingi361MagnesiumBy similarity1
Binding sitei361SubstrateBy similarity1
Metal bindingi386MagnesiumBy similarity1
Binding sitei386SubstrateBy similarity1
Active sitei411Proton acceptorBy similarity1
Binding sitei462SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • glycolytic process Source: UniProtKB-UniPathway
  • regulation of glucose metabolic process Source: FlyBase

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-70171. Glycolysis.
R-DME-70263. Gluconeogenesis.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:Eno
ORF Names:CG17654
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000579. Eno.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • lipid particle Source: FlyBase
  • phosphopyruvate hydratase complex Source: InterPro
  • plasma membrane Source: FlyBase

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340811 – 500EnolaseAdd BLAST500

Proteomic databases

PaxDbiP15007.
PRIDEiP15007.

Expressioni

Gene expression databases

BgeeiFBgn0000579.
ExpressionAtlasiP15007. differential.
GenevisibleiP15007. DM.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi59591. 86 interactors.
IntActiP15007. 5 interactors.
MINTiMINT-338625.
STRINGi7227.FBpp0077572.

Structurei

3D structure databases

ProteinModelPortaliP15007.
SMRiP15007.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni438 – 441Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
GeneTreeiENSGT00840000129817.
InParanoidiP15007.
KOiK01689.
OMAiEFMIIPV.
OrthoDBiEOG091G07NH.
PhylomeDBiP15007.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: P15007-1) [UniParc]FASTAAdd to basket
Also known as: C, D, E

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSWTASVFLR TSTTSMKFLR LRWPLPRIPQ NKSANVAPRF RSKSAVSQLS
60 70 80 90 100
SGFKFVQIRK STCDSNEMTI KAIKARQIYD SRGNPTVEVD LTTELGLFRA
110 120 130 140 150
AVPSGASTGV HEALELRDND KANYHGKSVL KAVGHVNDTL GPELIKANLD
160 170 180 190 200
VVDQASIDNF MIKLDGTENK SKFGANAILG VSLAVAKAGA AKKGVPLYKH
210 220 230 240 250
IADLAGNKEI ILPVPAFNVI NGGSHAGNKL AMQEFMILPT GATSFTEAMK
260 270 280 290 300
MGSEVYHHLK NVIKAKFGLD ATAVGDEGGF APNIQSNKEA LNLISDAIAK
310 320 330 340 350
AGYTGKIEIG MDVAASEFYK DGQYDLDFKN EKSDKSQWLP ADKLANLYQE
360 370 380 390 400
FIKDFPIVSI EDPFDQDHWE AWSNLTGCTD IQIVGDDLTV TNPKRIATAV
410 420 430 440 450
EKKACNCLLL KVNQIGTVTE SIAAHLLAKK NGWGTMVSHR SGETEDSFIG
460 470 480 490 500
DLVVGLSTGQ IKTGAPCRSE RLAKYNQILR IEEEIGAGVK FAGKSFRKPQ
Note: No experimental confirmation available.
Length:500
Mass (Da):54,310
Last modified:June 21, 2005 - v2
Checksum:i41CCA31672C01280
GO
Isoform A (identifier: P15007-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-67: Missing.

Show »
Length:433
Mass (Da):46,662
Checksum:iCD21F8AF69725444
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti335K → R in AAM48478 (PubMed:12537569).Curated1
Sequence conflicti335K → R in AAT47775 (Ref. 5) Curated1
Sequence conflicti349Q → K in CAA34895 (PubMed:2106662).Curated1
Sequence conflicti497R → G in CAA34895 (PubMed:2106662).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti380D → A in strain: SC96_47. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0141471 – 67Missing in isoform A. 2 PublicationsAdd BLAST67

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17034 Genomic DNA. Translation: CAA34895.1.
AE014134 Genomic DNA. Translation: AAF51344.2.
AE014134 Genomic DNA. Translation: AAN10455.1.
AE014134 Genomic DNA. Translation: AAN10456.1.
AE014134 Genomic DNA. Translation: AAN10457.1.
AE014134 Genomic DNA. Translation: AAN10458.1.
AY118449 mRNA. Translation: AAM48478.1.
BT014924 mRNA. Translation: AAT47775.1.
BT058002 mRNA. Translation: ACM16712.1.
BT072929 mRNA. Translation: ACN86077.1.
DQ864190 Genomic DNA. Translation: ABH06825.1.
DQ864191 Genomic DNA. Translation: ABH06826.1.
DQ864192 Genomic DNA. Translation: ABH06827.1.
DQ864193 Genomic DNA. Translation: ABH06828.1.
DQ864194 Genomic DNA. Translation: ABH06829.1.
DQ864195 Genomic DNA. Translation: ABH06830.1.
DQ864196 Genomic DNA. Translation: ABH06831.1.
DQ864197 Genomic DNA. Translation: ABH06832.1.
DQ864198 Genomic DNA. Translation: ABH06833.1.
DQ864199 Genomic DNA. Translation: ABH06834.1.
DQ864200 Genomic DNA. Translation: ABH06835.1.
DQ864201 Genomic DNA. Translation: ABH06836.1.
DQ864202 Genomic DNA. Translation: ABH06837.1.
DQ864203 Genomic DNA. Translation: ABH06838.1.
DQ864204 Genomic DNA. Translation: ABH06839.1.
DQ864205 Genomic DNA. Translation: ABH06840.1.
DQ864206 Genomic DNA. Translation: ABH06841.1.
DQ864207 Genomic DNA. Translation: ABH06842.1.
DQ864208 Genomic DNA. Translation: ABH06843.1.
DQ864209 Genomic DNA. Translation: ABH06844.1.
DQ864210 Genomic DNA. Translation: ABH06845.1.
DQ864211 Genomic DNA. Translation: ABH06846.1.
DQ864212 Genomic DNA. Translation: ABH06847.1.
DQ864213 Genomic DNA. Translation: ABH06848.1.
PIRiS07586.
RefSeqiNP_001162853.1. NM_001169382.2. [P15007-2]
NP_477421.1. NM_058073.5. [P15007-2]
NP_722721.1. NM_164431.3. [P15007-1]
NP_722722.1. NM_164432.3. [P15007-1]
NP_722723.1. NM_164433.3. [P15007-1]
NP_722724.1. NM_164434.3. [P15007-1]
UniGeneiDm.18435.

Genome annotation databases

EnsemblMetazoaiFBtr0077905; FBpp0077571; FBgn0000579. [P15007-1]
FBtr0077906; FBpp0077572; FBgn0000579. [P15007-1]
FBtr0077907; FBpp0077573; FBgn0000579. [P15007-1]
FBtr0077908; FBpp0077574; FBgn0000579. [P15007-1]
GeneIDi33351.
KEGGidme:Dmel_CG17654.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17034 Genomic DNA. Translation: CAA34895.1.
AE014134 Genomic DNA. Translation: AAF51344.2.
AE014134 Genomic DNA. Translation: AAN10455.1.
AE014134 Genomic DNA. Translation: AAN10456.1.
AE014134 Genomic DNA. Translation: AAN10457.1.
AE014134 Genomic DNA. Translation: AAN10458.1.
AY118449 mRNA. Translation: AAM48478.1.
BT014924 mRNA. Translation: AAT47775.1.
BT058002 mRNA. Translation: ACM16712.1.
BT072929 mRNA. Translation: ACN86077.1.
DQ864190 Genomic DNA. Translation: ABH06825.1.
DQ864191 Genomic DNA. Translation: ABH06826.1.
DQ864192 Genomic DNA. Translation: ABH06827.1.
DQ864193 Genomic DNA. Translation: ABH06828.1.
DQ864194 Genomic DNA. Translation: ABH06829.1.
DQ864195 Genomic DNA. Translation: ABH06830.1.
DQ864196 Genomic DNA. Translation: ABH06831.1.
DQ864197 Genomic DNA. Translation: ABH06832.1.
DQ864198 Genomic DNA. Translation: ABH06833.1.
DQ864199 Genomic DNA. Translation: ABH06834.1.
DQ864200 Genomic DNA. Translation: ABH06835.1.
DQ864201 Genomic DNA. Translation: ABH06836.1.
DQ864202 Genomic DNA. Translation: ABH06837.1.
DQ864203 Genomic DNA. Translation: ABH06838.1.
DQ864204 Genomic DNA. Translation: ABH06839.1.
DQ864205 Genomic DNA. Translation: ABH06840.1.
DQ864206 Genomic DNA. Translation: ABH06841.1.
DQ864207 Genomic DNA. Translation: ABH06842.1.
DQ864208 Genomic DNA. Translation: ABH06843.1.
DQ864209 Genomic DNA. Translation: ABH06844.1.
DQ864210 Genomic DNA. Translation: ABH06845.1.
DQ864211 Genomic DNA. Translation: ABH06846.1.
DQ864212 Genomic DNA. Translation: ABH06847.1.
DQ864213 Genomic DNA. Translation: ABH06848.1.
PIRiS07586.
RefSeqiNP_001162853.1. NM_001169382.2. [P15007-2]
NP_477421.1. NM_058073.5. [P15007-2]
NP_722721.1. NM_164431.3. [P15007-1]
NP_722722.1. NM_164432.3. [P15007-1]
NP_722723.1. NM_164433.3. [P15007-1]
NP_722724.1. NM_164434.3. [P15007-1]
UniGeneiDm.18435.

3D structure databases

ProteinModelPortaliP15007.
SMRiP15007.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi59591. 86 interactors.
IntActiP15007. 5 interactors.
MINTiMINT-338625.
STRINGi7227.FBpp0077572.

Proteomic databases

PaxDbiP15007.
PRIDEiP15007.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0077905; FBpp0077571; FBgn0000579. [P15007-1]
FBtr0077906; FBpp0077572; FBgn0000579. [P15007-1]
FBtr0077907; FBpp0077573; FBgn0000579. [P15007-1]
FBtr0077908; FBpp0077574; FBgn0000579. [P15007-1]
GeneIDi33351.
KEGGidme:Dmel_CG17654.

Organism-specific databases

CTDi33351.
FlyBaseiFBgn0000579. Eno.

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
GeneTreeiENSGT00840000129817.
InParanoidiP15007.
KOiK01689.
OMAiEFMIIPV.
OrthoDBiEOG091G07NH.
PhylomeDBiP15007.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.
ReactomeiR-DME-70171. Glycolysis.
R-DME-70263. Gluconeogenesis.

Miscellaneous databases

ChiTaRSiEno. fly.
GenomeRNAii33351.
PROiP15007.

Gene expression databases

BgeeiFBgn0000579.
ExpressionAtlasiP15007. differential.
GenevisibleiP15007. DM.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENO_DROME
AccessioniPrimary (citable) accession number: P15007
Secondary accession number(s): A4UZY9
, A5XD39, A5XD43, B9EQS7, C0PV73, Q8IPX8, Q8MT10, Q9VQ38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 21, 2005
Last modified: March 15, 2017
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.