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Protein

5-methylcytosine-specific restriction enzyme B

Gene

mcrB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes N4- and C5-methylcytosine (and 5-hydroxy-methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Isoform 33 kDa is less active than isoform 51 kDa and may play a role in regulating the activity of isoform 51 kDa by competing with it in DNA and protein binding abilities.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi201 – 208GTPSequence analysis8
Nucleotide bindingi300 – 303GTPSequence analysis4
Nucleotide bindingi333 – 336GTPSequence analysis4

GO - Molecular functioni

  • ATPase activity Source: InterPro
  • ATP binding Source: InterPro
  • DNA binding Source: EcoliWiki
  • GTPase activity Source: EcoliWiki
  • GTP binding Source: UniProtKB-KW
  • restriction endodeoxyribonuclease activity Source: EcoliWiki

GO - Biological processi

  • DNA catabolic process Source: EcoliWiki
  • DNA restriction-modification system Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Endonuclease, Hydrolase, Nuclease
Biological processRestriction system
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10574-MONOMER
MetaCyc:EG10574-MONOMER

Protein family/group databases

REBASEi2865 EcoKMcrBC

Names & Taxonomyi

Protein namesi
Recommended name:
5-methylcytosine-specific restriction enzyme B (EC:3.1.21.-)
Alternative name(s):
EcoKMcrBC
Gene namesi
Name:mcrB
Synonyms:rglB
Ordered Locus Names:b4346, JW5871
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10574 mcrB

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000303471 – 4595-methylcytosine-specific restriction enzyme BAdd BLAST459

Proteomic databases

PaxDbiP15005
PRIDEiP15005

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
nnrP318063EBI-552513,EBI-554188

Protein-protein interaction databases

BioGridi4262765, 164 interactors
DIPiDIP-10168N
IntActiP15005, 3 interactors
STRINGi316407.85677086

Structurei

Secondary structure

1459
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 17Combined sources13
Beta strandi24 – 26Combined sources3
Beta strandi28 – 30Combined sources3
Beta strandi33 – 53Combined sources21
Beta strandi59 – 70Combined sources12
Helixi71 – 73Combined sources3
Beta strandi75 – 82Combined sources8
Beta strandi84 – 86Combined sources3
Helixi102 – 110Combined sources9
Beta strandi120 – 127Combined sources8
Helixi128 – 130Combined sources3
Helixi134 – 152Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SSCX-ray2.10A/B1-161[»]
3SSDX-ray2.20A/B1-161[»]
3SSEX-ray2.70A/B1-161[»]
ProteinModelPortaliP15005
SMRiP15005
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105FBQ Bacteria
COG1401 LUCA
HOGENOMiHOG000081315
InParanoidiP15005
KOiK07452
OMAiKAYLMES
PhylomeDBiP15005

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR011704 ATPase_dyneun-rel_AAA
IPR021961 DUF3578
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF07728 AAA_5, 1 hit
PF12102 DUF3578, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 51 kDa (identifier: P15005-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESIQPWIEK FIKQAQQQRS QSTKDYPTSY RNLRVKLSFG YGNFTSIPWF
60 70 80 90 100
AFLGEGQEAS NGIYPVILYY KDFDELVLAY GISDTNEPHA QWQFSSDIPK
110 120 130 140 150
TIAEYFQATS GVYPKKYGQS YYACSQKVSQ GIDYTRFASM LDNIINDYKL
160 170 180 190 200
IFNSGKSVIP PMSKTESYCL EDALNDLFIP ETTIETILKR LTIKKNIILQ
210 220 230 240 250
GPPGVGKTFV ARRLAYLLTG EKAPQRVNMV QFHQSYSYED FIQGYRPNGV
260 270 280 290 300
GFRRKDGIFY NFCQQAKEQP EKKYIFIIDE INRANLSKVF GEVMMLMEHD
310 320 330 340 350
KRGENWSVPL TYSENDEERF YVPENVYIIG LMNTADRSLA VVDYALRRRF
360 370 380 390 400
SFIDIEPGFD TPQFRNFLLN KKAEPSFVES LCQKMNELNQ EISKEATILG
410 420 430 440 450
KGFRIGHSYF CCGLEDGTSP DTQWLNEIVM TDIAPLLEEY FFDDPYKQQK

WTNKLLGDS
Length:459
Mass (Da):53,157
Last modified:December 15, 1998 - v3
Checksum:iC8167978B0D720CA
GO
Isoform 33 kDa (identifier: P15005-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-161: Missing.

Show »
Length:298
Mass (Da):34,660
Checksum:iB05DE88C3CAB9321
GO

Sequence cautioni

The sequence AAA24145 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAA97243 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti186 – 212TILKR…TFVAR → SDTQTINHQKKYYPPGAARR WKNLCCT in AAA24142 (PubMed:2649480).CuratedAdd BLAST27
Sequence conflicti224 – 232PQRVNMVQF → RTKYGSV in AAA24142 (PubMed:2649480).Curated9
Sequence conflicti245 – 259YRPNG…KDGIF → IVRMASASVKTHI (PubMed:2649480).CuratedAdd BLAST15

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188681 – 161Missing in isoform 33 kDa. CuratedAdd BLAST161

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58752 Genomic DNA Translation: AAA24145.1 Different initiation.
M24927 Genomic DNA Translation: AAA24142.1
U14003 Genomic DNA Translation: AAA97243.1 Different initiation.
U00096 Genomic DNA Translation: AAC77302.2
AP009048 Genomic DNA Translation: BAE78336.1
PIRiA36708 XYECMB
RefSeqiNP_418766.4, NC_000913.3
WP_000443951.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77302; AAC77302; b4346
BAE78336; BAE78336; BAE78336
GeneIDi949122
KEGGiecj:JW5871
eco:b4346
PATRICifig|511145.12.peg.4492

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiMCRB_ECOLI
AccessioniPrimary (citable) accession number: P15005
Secondary accession number(s): Q2M5X0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: December 15, 1998
Last modified: March 28, 2018
This is version 159 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health