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Protein

5-methylcytosine-specific restriction enzyme B

Gene

mcrB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes N4- and C5-methylcytosine (and 5-hydroxy-methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Isoform 33 kDa is less active than isoform 51 kDa and may play a role in regulating the activity of isoform 51 kDa by competing with it in DNA and protein binding abilities.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi201 – 2088GTPSequence analysis
Nucleotide bindingi300 – 3034GTPSequence analysis
Nucleotide bindingi333 – 3364GTPSequence analysis

GO - Molecular functioni

  • ATPase activity Source: InterPro
  • ATP binding Source: InterPro
  • DNA binding Source: EcoliWiki
  • GTPase activity Source: EcoliWiki
  • GTP binding Source: UniProtKB-KW
  • restriction endodeoxyribonuclease activity Source: EcoliWiki

GO - Biological processi

  • DNA catabolic process Source: EcoliWiki
  • DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

DNA-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10574-MONOMER.
ECOL316407:JW5871-MONOMER.
MetaCyc:EG10574-MONOMER.

Protein family/group databases

REBASEi2865. EcoKMcrBC.

Names & Taxonomyi

Protein namesi
Recommended name:
5-methylcytosine-specific restriction enzyme B (EC:3.1.21.-)
Alternative name(s):
EcoKMcrBC
Gene namesi
Name:mcrB
Synonyms:rglB
Ordered Locus Names:b4346, JW5871
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10574. mcrB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4594595-methylcytosine-specific restriction enzyme BPRO_0000030347Add
BLAST

Proteomic databases

PaxDbiP15005.
PRIDEiP15005.

Interactioni

Protein-protein interaction databases

BioGridi4262765. 160 interactions.
DIPiDIP-10168N.
IntActiP15005. 3 interactions.
MINTiMINT-1286238.
STRINGi511145.b4346.

Structurei

Secondary structure

1
459
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1713Combined sources
Beta strandi24 – 263Combined sources
Beta strandi28 – 303Combined sources
Beta strandi33 – 5321Combined sources
Beta strandi59 – 7012Combined sources
Helixi71 – 733Combined sources
Beta strandi75 – 828Combined sources
Beta strandi84 – 863Combined sources
Helixi102 – 1109Combined sources
Beta strandi120 – 1278Combined sources
Helixi128 – 1303Combined sources
Helixi134 – 15219Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SSCX-ray2.10A/B1-161[»]
3SSDX-ray2.20A/B1-161[»]
3SSEX-ray2.70A/B1-161[»]
ProteinModelPortaliP15005.
SMRiP15005. Positions 2-155, 195-220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105FBQ. Bacteria.
COG1401. LUCA.
HOGENOMiHOG000081315.
InParanoidiP15005.
KOiK07452.
OMAiKAYLMES.
OrthoDBiEOG69GZQ6.
PhylomeDBiP15005.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011704. ATPase_dyneun-rel_AAA.
IPR021961. DUF3578.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF07728. AAA_5. 1 hit.
PF12102. DUF3578. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 51 kDa (identifier: P15005-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESIQPWIEK FIKQAQQQRS QSTKDYPTSY RNLRVKLSFG YGNFTSIPWF
60 70 80 90 100
AFLGEGQEAS NGIYPVILYY KDFDELVLAY GISDTNEPHA QWQFSSDIPK
110 120 130 140 150
TIAEYFQATS GVYPKKYGQS YYACSQKVSQ GIDYTRFASM LDNIINDYKL
160 170 180 190 200
IFNSGKSVIP PMSKTESYCL EDALNDLFIP ETTIETILKR LTIKKNIILQ
210 220 230 240 250
GPPGVGKTFV ARRLAYLLTG EKAPQRVNMV QFHQSYSYED FIQGYRPNGV
260 270 280 290 300
GFRRKDGIFY NFCQQAKEQP EKKYIFIIDE INRANLSKVF GEVMMLMEHD
310 320 330 340 350
KRGENWSVPL TYSENDEERF YVPENVYIIG LMNTADRSLA VVDYALRRRF
360 370 380 390 400
SFIDIEPGFD TPQFRNFLLN KKAEPSFVES LCQKMNELNQ EISKEATILG
410 420 430 440 450
KGFRIGHSYF CCGLEDGTSP DTQWLNEIVM TDIAPLLEEY FFDDPYKQQK

WTNKLLGDS
Length:459
Mass (Da):53,157
Last modified:December 15, 1998 - v3
Checksum:iC8167978B0D720CA
GO
Isoform 33 kDa (identifier: P15005-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-161: Missing.

Show »
Length:298
Mass (Da):34,660
Checksum:iB05DE88C3CAB9321
GO

Sequence cautioni

The sequence AAA24145.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAA97243.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti186 – 21227TILKR…TFVAR → SDTQTINHQKKYYPPGAARR WKNLCCT in AAA24142 (PubMed:2649480).CuratedAdd
BLAST
Sequence conflicti224 – 2329PQRVNMVQF → RTKYGSV in AAA24142 (PubMed:2649480).Curated
Sequence conflicti245 – 25915YRPNG…KDGIF → IVRMASASVKTHI (PubMed:2649480).CuratedAdd
BLAST

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 161161Missing in isoform 33 kDa. CuratedVSP_018868Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58752 Genomic DNA. Translation: AAA24145.1. Different initiation.
M24927 Genomic DNA. Translation: AAA24142.1.
U14003 Genomic DNA. Translation: AAA97243.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77302.2.
AP009048 Genomic DNA. Translation: BAE78336.1.
PIRiA36708. XYECMB.
RefSeqiNP_418766.4. NC_000913.3.
WP_000443951.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77302; AAC77302; b4346.
BAE78336; BAE78336; BAE78336.
GeneIDi949122.
KEGGiecj:JW5871.
eco:b4346.
PATRICi32124298. VBIEscCol129921_4492.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58752 Genomic DNA. Translation: AAA24145.1. Different initiation.
M24927 Genomic DNA. Translation: AAA24142.1.
U14003 Genomic DNA. Translation: AAA97243.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77302.2.
AP009048 Genomic DNA. Translation: BAE78336.1.
PIRiA36708. XYECMB.
RefSeqiNP_418766.4. NC_000913.3.
WP_000443951.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SSCX-ray2.10A/B1-161[»]
3SSDX-ray2.20A/B1-161[»]
3SSEX-ray2.70A/B1-161[»]
ProteinModelPortaliP15005.
SMRiP15005. Positions 2-155, 195-220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262765. 160 interactions.
DIPiDIP-10168N.
IntActiP15005. 3 interactions.
MINTiMINT-1286238.
STRINGi511145.b4346.

Protein family/group databases

REBASEi2865. EcoKMcrBC.

Proteomic databases

PaxDbiP15005.
PRIDEiP15005.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77302; AAC77302; b4346.
BAE78336; BAE78336; BAE78336.
GeneIDi949122.
KEGGiecj:JW5871.
eco:b4346.
PATRICi32124298. VBIEscCol129921_4492.

Organism-specific databases

EchoBASEiEB0569.
EcoGeneiEG10574. mcrB.

Phylogenomic databases

eggNOGiENOG4105FBQ. Bacteria.
COG1401. LUCA.
HOGENOMiHOG000081315.
InParanoidiP15005.
KOiK07452.
OMAiKAYLMES.
OrthoDBiEOG69GZQ6.
PhylomeDBiP15005.

Enzyme and pathway databases

BioCyciEcoCyc:EG10574-MONOMER.
ECOL316407:JW5871-MONOMER.
MetaCyc:EG10574-MONOMER.

Miscellaneous databases

PROiP15005.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011704. ATPase_dyneun-rel_AAA.
IPR021961. DUF3578.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF07728. AAA_5. 1 hit.
PF12102. DUF3578. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic and sequence organization of the mcrBC locus of Escherichia coli K-12."
    Dila D., Sutherland E., Moran L., Slatko B., Raleigh E.A.
    J. Bacteriol. 172:4888-4900(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Nucleotide sequence of the McrB region of Escherichia coli K-12 and evidence for two independent translational initiation sites at the mcrB locus."
    Ross T.K., Achberger E.C., Braymer H.D.
    J. Bacteriol. 171:1974-1981(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Purification and N-terminal amino acid sequences of two polypeptides encoded by the mcrB gene from Escherichia coli K-12."
    Zheng L., Wang X., Braymer H.D.
    Gene 112:97-100(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
  7. "McrB: a prokaryotic protein specifically recognizing DNA containing modified cytosine residues."
    Krueger T., Wild C., Noyer-Weidner M.
    EMBO J. 14:2661-2669(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiMCRB_ECOLI
AccessioniPrimary (citable) accession number: P15005
Secondary accession number(s): Q2M5X0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: December 15, 1998
Last modified: July 6, 2016
This is version 148 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.