P15005 (MCRB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methylcytosine-specific restriction enzyme B
EC=3.1.21.-

Alternative name(s):
EcoKMcrBC

Gene names

Name:	mcrB
Synonyms:	rglB
Ordered Locus Names:	b4346, JW5871

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Recognizes N4- and C5-methylcytosine (and 5-hydroxy-methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Isoform 33 kDa is less active than isoform 51 kDa and may play a role in regulating the activity of isoform 51 kDa by competing with it in DNA and protein binding abilities.

Sequence caution

The sequence AAA24145.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAA97243.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Restriction system
Coding sequence diversity	Alternative initiation
Ligand	DNA-binding GTP-binding Nucleotide-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro ATPase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
yjeF	P31806	1	EBI-552513,EBI-554188

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform 51 kDa (identifier: P15005-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 33 kDa (identifier: P15005-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-161: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	5-methylcytosine-specific restriction enzyme B		PRO_0000030347
Regions
	Nucleotide binding	201 – 208	8	GTP Potential
	Nucleotide binding	300 – 303	4	GTP Potential
	Nucleotide binding	333 – 336	4	GTP Potential
Natural variations
	Alternative sequence	1 – 161	161	Missing in isoform 33 kDa.		VSP_018868
Experimental info
	Sequence conflict	186 – 212	27	TILKR…TFVAR → SDTQTINHQKKYYPPGAARR WKNLCCT in AAA24142. Ref.2
	Sequence conflict	224 – 232	9	PQRVNMVQF → RTKYGSV in AAA24142. Ref.2
	Sequence conflict	245 – 259	15	YRPNG…KDGIF → IVRMASASVKTHI Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 51 kDa [UniParc]. Last modified December 15, 1998. Version 3. Checksum: C8167978B0D720CA	FASTA	459	53,157
	10 20 30 40 50 60 MESIQPWIEK FIKQAQQQRS QSTKDYPTSY RNLRVKLSFG YGNFTSIPWF AFLGEGQEAS 70 80 90 100 110 120 NGIYPVILYY KDFDELVLAY GISDTNEPHA QWQFSSDIPK TIAEYFQATS GVYPKKYGQS 130 140 150 160 170 180 YYACSQKVSQ GIDYTRFASM LDNIINDYKL IFNSGKSVIP PMSKTESYCL EDALNDLFIP 190 200 210 220 230 240 ETTIETILKR LTIKKNIILQ GPPGVGKTFV ARRLAYLLTG EKAPQRVNMV QFHQSYSYED 250 260 270 280 290 300 FIQGYRPNGV GFRRKDGIFY NFCQQAKEQP EKKYIFIIDE INRANLSKVF GEVMMLMEHD 310 320 330 340 350 360 KRGENWSVPL TYSENDEERF YVPENVYIIG LMNTADRSLA VVDYALRRRF SFIDIEPGFD 370 380 390 400 410 420 TPQFRNFLLN KKAEPSFVES LCQKMNELNQ EISKEATILG KGFRIGHSYF CCGLEDGTSP 430 440 450 DTQWLNEIVM TDIAPLLEEY FFDDPYKQQK WTNKLLGDS
	Isoform 33 kDa [UniParc]. Checksum: B05DE88C3CAB9321	FASTA	298	34,660
	10 20 30 40 50 60 MSKTESYCLE DALNDLFIPE TTIETILKRL TIKKNIILQG PPGVGKTFVA RRLAYLLTGE 70 80 90 100 110 120 KAPQRVNMVQ FHQSYSYEDF IQGYRPNGVG FRRKDGIFYN FCQQAKEQPE KKYIFIIDEI 130 140 150 160 170 180 NRANLSKVFG EVMMLMEHDK RGENWSVPLT YSENDEERFY VPENVYIIGL MNTADRSLAV 190 200 210 220 230 240 VDYALRRRFS FIDIEPGFDT PQFRNFLLNK KAEPSFVESL CQKMNELNQE ISKEATILGK 250 260 270 280 290 GFRIGHSYFC CGLEDGTSPD TQWLNEIVMT DIAPLLEEYF FDDPYKQQKW TNKLLGDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic and sequence organization of the mcrBC locus of Escherichia coli K-12." Dila D., Sutherland E., Moran L., Slatko B., Raleigh E.A. J. Bacteriol. 172:4888-4900(1990) [PubMed: 2203735] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Nucleotide sequence of the McrB region of Escherichia coli K-12 and evidence for two independent translational initiation sites at the mcrB locus." Ross T.K., Achberger E.C., Braymer H.D. J. Bacteriol. 171:1974-1981(1989) [PubMed: 2649480] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Purification and N-terminal amino acid sequences of two polypeptides encoded by the mcrB gene from Escherichia coli K-12." Zheng L., Wang X., Braymer H.D. Gene 112:97-100(1992) [PubMed: 1312983] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.
[7]	"McrB: a prokaryotic protein specifically recognizing DNA containing modified cytosine residues." Krueger T., Wild C., Noyer-Weidner M. EMBO J. 14:2661-2669(1995) [PubMed: 7781618] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58752 Genomic DNA. Translation: AAA24145.1. Different initiation. M24927 Genomic DNA. Translation: AAA24142.1. U14003 Genomic DNA. Translation: AAA97243.1. Different initiation. U00096 Genomic DNA. Translation: AAC77302.2. AP009048 Genomic DNA. Translation: BAE78336.1.
PIR	XYECMB. A36708.
RefSeq	NP_418766.4. NC_000913.2.
3D structure databases
ProteinModelPortal	P15005.
SMR	P15005. Positions 192-355.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10168N.
IntAct	P15005. 2 interactions.
MINT	MINT-1286238.
Protein family/group databases
REBASE	13377. EcoW3110McrBCP. 2865. EcoKMcrBC.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	949122.
GenomeReviews	Gene locus JW5871 in contig AP009048_GR. Gene locus b4346 in contig U00096_GR.
KEGG	ecj:JW5871. eco:b4346.
PATRIC	32124298. VBIEscCol129921_4492.
Organism-specific databases
EchoBASE	EB0569.
EcoGene	EG10574. mcrB.
Phylogenomic databases
eggNOG	COG1401.
GeneTree	EBGT00050000012024.
HOGENOM	HBG474543.
OMA	ESIQAWI.
PhylomeDB	P15005.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10574-MONOMER. MetaCyc:EG10574-MONOMER.
Gene expression databases
Genevestigator	P15005.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR011704. ATPase_dyneun-rel_AAA. IPR021961. DUF3578. [Graphical view]
KO	K07452.
Pfam	PF07728. AAA_5. 1 hit. PF12102. DUF3578. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCRB_ECOLI

Accession

Primary (citable) accession number: P15005
Secondary accession number(s): Q2M5X0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	December 15, 1998
Last modified:	January 25, 2012
This is version 111 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program