P14998 (AGNO_POVBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Agnoprotein Alternative name(s): Agno
Organism	BK polyomavirus (strain AS) (BKPyV) [Complete proteome]
Taxonomic identifier	10631 [NCBI]
Taxonomic lineage	Viruses › dsDNA viruses, no RNA stage › Polyomaviridae › Polyomavirus
Virus host	Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	74 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Alters the structure of the nuclear envelope by interacting with host CBX5 and disrupting CBX5 association with LBR. Involved in the perinuclear-nuclear localization of the capsid protein VP1 during virion assembly and maturation. Plays an important role in the release of progeny virions from infected cells and in viral propagation, probably by acting as a viral ionic channel in the host plasma membrane. Allows influx of extracellular calcium ions in the host cell. May contribute to viral genome transcription and translation of viral late proteins By similarity.
Subunit structure	Homooligomer. Interacts with VP1 By similarity. Interacts with large T antigen; this interaction may impact upon the activity of T-antigen on the control of viral gene transcription and replication. Interacts with small t antigen. Interacts with host CBX5; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope By similarity.
Subcellular location	Host cytoplasm Potential. Host nucleus membrane; Single-pass type II membrane protein Potential. Host rough endoplasmic reticulum membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential. Note: Mostly perinuclear By similarity.
Post-translational modification	Phosphorylated by host kinase. Phosphorylation segregates agnoprotein in cytoplasm, whereas unphosphorylated agnoprotein migrate to the nucleus By similarity.
Sequence similarities	Belongs to the polyomavirus agnoprotein family.

Ontologies

Keywords
Biological process	Host-virus interaction Ion transport Transport
Cellular component	Host cell membrane Host cytoplasm Host endoplasmic reticulum Host membrane Host nucleus Membrane
Coding sequence diversity	Alternative initiation Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Ionic channel Viral ionic channel
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	host cell nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell rough endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro ion channel activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing and alternative initiation. [Align] [Select]

Isoform Agno (identifier: P14998-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced by alternative initiation of the late mRNA.

Isoform VP2 (identifier: P14997-1)

Also known as: Minor capsid protein VP2;

The sequence of this isoform can be found in the external entry P14997.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative splicing of the late mRNA.

Isoform VP3 (identifier: P14997-2)

Also known as: Minor capsid protein VP3;

The sequence of this isoform can be found in the external entry P14997.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative initiation at Met-120 of isoform VP2.

Isoform VP4 (identifier: P14997-3)

Also known as: Viroporin VP4;

The sequence of this isoform can be found in the external entry P14997.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative initiation at Met-229 of isoform VP2.

Isoform VP1 (identifier: P14996-1)

The sequence of this isoform can be found in the external entry P14996.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by alternative splicing of the late mRNA.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 74	74	Agnoprotein		PRO_0000115030
Regions
	Topological domain	1 – 35	35	Cytoplasmic Potential
	Transmembrane	36 – 52	17	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	53 – 74	22	Extracellular Potential
Amino acid modifications
	Modified residue	15	1	Phosphoserine; by host By similarity
	Modified residue	19	1	Phosphoserine; by host By similarity
	Modified residue	29	1	Phosphothreonine; by host By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform Agno [UniParc]. Last modified April 1, 1990. Version 1. Checksum: B77365F6878718BB	FASTA	74	8,328
	10 20 30 40 50 60 MFCEPKNLVV LRQLSRQASV KVGKTWTGTK KRAQRIFIFI LELLLEFCRG EDSVDGKNKS 70 TTALPAVKDS VKDS
	Isoform VP2 (Minor capsid protein VP2) [UniParc]. See P14997.		–	–
	Isoform VP3 (Minor capsid protein VP3) [UniParc]. See P14997.		–	–
	Isoform VP4 (Viroporin VP4) [UniParc]. See P14997.		–	–
	Isoform VP1 [UniParc]. See P14996.		–	–

References

[1]	"Nucleotide sequence of the human polyomavirus AS virus, an antigenic variant of BK virus." Tavis J.E., Walker D.L., Gardner S.D., Frisque R.J. J. Virol. 63:901-911(1989) [PubMed: 2536111] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M23122 Genomic DNA. Translation: AAA46879.1.
PIR	DNVPAS. C33278.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002643. Polyoma_agno. [Graphical view]
Pfam	PF01736. Polyoma_agno. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGNO_POVBA

Accession

Primary (citable) accession number: P14998

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	October 19, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program