Minor capsid protein VP2 - BK polyomavirus (strain AS) (BKPyV)

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Reviewed, UniProtKB/Swiss-Prot P14997 (VP2_POVBA)

Last modified January 19, 2010. Version 52. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names	Recommended name: Minor capsid protein VP2 Alternative name(s): Minor structural protein VP2
Organism	BK polyomavirus (strain AS) (BKPyV) [Complete proteome]
Taxonomic identifier	10631 [NCBI]
Taxonomic lineage	Viruses › dsDNA viruses, no RNA stage › Polyomaviridae › Polyomavirus
Virus host	Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Isoform VP2 is a structural protein that resides within the core of the capsid surrounded by 72 VP1 pentamers. Participates in host cell receptor binding together with VP1. Following virus entry, VP2 and VP3 form oligomers and integrate into the endoplasmic reticulum membrane. Heterooligomer VP2-VP3 may create a viroporin for transporting the viral genome across the endoplasmic reticulum membrane. Plays a role in virion assembly within the nucleus in particular through a DNA-binding domain located in the C-terminal region. A N-terminal myristoylation suggests a scaffold function for virion assembly By similarity. Isoform VP3 is a structural protein that resides within the core of the capsid surrounded by 72 VP1 pentamers. Following virus entry, VP2 and VP3 form oligomers and integrate into the endoplasmic reticulum membrane. Heterooligomer VP2-VP3 may create a viroporin for transporting the viral genome across the endoplasmic reticulum membrane. Isoform VP3 plays a role in virion assembly within the nucleus. May participate in host cell lysis when associated with VP4 By similarity. Isoform VP4 is expressed after the late structural proteins and is not incorporated into the mature virion. May associate with VP3 to initiate host cell lysis and viral progeny release By similarity.
Subunit structure	Isoform VP2 forms homooligomers, and heterooligomers with VP3 in the endoplasmic reticulum membrane. Isoform VP2 interacts (via D1 domain) with VP1. Isoform VP3 interacts (via D1 domain) with VP1 By similarity.
Subcellular location	Isoform VP2: Virion. Host nucleus. Host endoplasmic reticulum. Host endoplasmic reticulum membrane By similarity. Isoform VP3: Virion. Host nucleus. Host endoplasmic reticulum. Host endoplasmic reticulum membrane By similarity. Isoform VP4: Host nucleus By similarity.
Sequence similarities	Belongs to the polyomaviruses capsid protein VP2 family.

Ontologies

Keywords
Cellular component	Capsid protein Host endoplasmic reticulum Host membrane Host nucleus Membrane Virion
Coding sequence diversity	Alternative initiation Alternative splicing
Developmental stage	Late protein
Domain	Transmembrane
Ligand	DNA-binding
PTM	Lipoprotein Myristate
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	host cell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing and alternative initiation. [Align] [Select]

Isoform VP2 (identifier: P14997-1) Also known as: Minor capsid protein VP2; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by alternative splicing of the late mRNA.

Isoform VP3 (identifier: P14997-2) Also known as: Minor capsid protein VP3; The sequence of this isoform differs from the canonical sequence as follows: 1-119: Missing.
Note: Produced by alternative initiation at Met-120 of isoform VP2.

Isoform VP4 (identifier: P14997-3) The sequence of this isoform differs from the canonical sequence as follows: 1-228: Missing.
Note: Produced by alternative initiation at Met-228 of isoform VP2.

Isoform VP1 (identifier: P14996-1) The sequence of this isoform can be found in the external entry P14996-1. Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by alternative splicing of the late mRNA.

Isoform Agno (identifier: P14998-1) The sequence of this isoform can be found in the external entry P14998-1. Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by alternative initiation of the late mRNA leader region.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

Removed; by host By similarity

Chain

350

Minor capsid protein VP2

PRO_0000039203

Regions

Transmembrane

21

Potential

Region

36

D1 By similarity

Region

40

DNA-binding By similarity

Motif

9

Nuclear localization signal By similarity

Compositional bias

81

Ala-rich

Amino acid modifications

Lipidation

1

N-myristoyl glycine; by host By similarity

Natural variations

Alternative sequence

228

Missing in isoform VP4.

VSP_036013

Alternative sequence

119

Missing in isoform VP3.

VSP_018915

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform VP2 (Minor capsid protein VP2) [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: D926105B0D1A4130
Show »

351

38,303

        10         20         30         40         50         60 
MGAALALLGD LVASVSEAAA ATGFSVAEIA AGEAAAAIEV QIASLATVEG ITTTSEAIAA 

        70         80         90        100        110        120 
IGLTPQTYAV IAGAPGAIAG FAALIQTVTG ISSLAQVGYR FFSDWDHKVS TVGLYQQSGM 

       130        140        150        160        170        180 
ALELFNPDEY YDILFPGVNT FVNNIQYLDP RHWGPSLFAT ISQALWHVIR DDIPAITSQE 

       190        200        210        220        230        240 
LQRRTERFFR DSLARFLEET TWTIVNAPIN FYNYIQDYYS NLSPIRPSMV RQVAEREGTH 

       250        260        270        280        290        300 
VNFGHTYSID NADSIEEVTQ RMDLRNKESV HSGEFIEKTI APGGANQRTA PQWMLPLLLG 

       310        320        330        340        350 
LYGTVTPALE AYEDGPNQKK RRVSRGSSQK AKGTRASAKT TNKRRSRSSR S

Isoform VP3 (Minor capsid protein VP3).

Checksum: 0C5A5047989D099A
Show »

232

26,648

Isoform VP4.

Checksum: 9F9E6E9E28BF4B82
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123

13,686

Isoform VP1.

–

–

Isoform Agno.

–

–

References

[1]	"Nucleotide sequence of the human polyomavirus AS virus, an antigenic variant of BK virus." Tavis J.E., Walker D.L., Gardner S.D., Frisque R.J. J. Virol. 63:901-911(1989) [PubMed: 2536111] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M23122 Genomic DNA. Translation: AAA46880.1. M23122 Genomic DNA. Translation: AAA46881.1.
PIR	VVVPAS. E33278.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001070. Polyoma_coat_VP2. [Graphical view]
Pfam	PF00761. Polyoma_coat2. 1 hit. [Graphical view]
PIRSF	PIRSF003377. Polyoma_coat2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

VP2_POVBA

Accession

Primary (citable) accession number: P14997

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 23, 2007
Last modified:	January 19, 2010
This is version 52 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents