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Protein

Replication-associated protein A

Gene

C1

Organism
Maize streak virus genotype A (isolate South Africa) (MSV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Implicated in enhancement of V-sense gene expression. Acts a an inhibitor of C-sense gene transcription (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi52Divalent metal cationSequence analysis1
Metal bindingi60Divalent metal cationSequence analysis1
Metal bindingi62Divalent metal cationSequence analysis1
Active sitei100For DNA cleavage activityBy similarity1
Metal bindingi104Divalent metal cationSequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Endonuclease, Hydrolase, Nuclease, Repressor
Biological processG1/S host cell cycle checkpoint dysregulation by virus, Host-virus interaction, Modulation of host cell cycle by virus
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated protein A (EC:3.1.21.-)
Short name:
RepA
Gene namesi
ORF Names:C1
OrganismiMaize streak virus genotype A (isolate South Africa) (MSV)
Taxonomic identifieri10824 [NCBI]
Taxonomic lineageiVirusesssDNA virusesGeminiviridaeMastrevirus
Virus hostiAvena sativa (Oat) [TaxID: 4498]
Axonopus compressus [TaxID: 217170]
Brachiaria deflexa [TaxID: 240436]
Cenchrus polystachios [TaxID: 281129]
Coix lacryma-jobi (Job's tears) [TaxID: 4505]
Dactyloctenium aegyptium [TaxID: 270102]
Digitaria [TaxID: 66017]
Echinochloa colona [TaxID: 90396]
Eleusine coracana (Indian finger millet) (Ragi) [TaxID: 4511]
Eleusine indica (Goosegrass) (Cynosurus indicus) [TaxID: 29674]
Hordeum vulgare (Barley) [TaxID: 4513]
Megathyrsus maximus [TaxID: 59788]
Melinis repens (Natal redtop) (Rhynchelytrum repens) [TaxID: 29709]
Oryza glaberrima (African rice) [TaxID: 4538]
Oryza sativa (Rice) [TaxID: 4530]
Paspalum conjugatum (Hilo grass) [TaxID: 158143]
Paspalum notatum (Bahia grass) [TaxID: 147272]
Paspalum scrobiculatum [TaxID: 173849]
Pennisetum americanum (Pearl millet) (Pennisetum glaucum) [TaxID: 4543]
Rottboellia cochinchinensis [TaxID: 300125]
Saccharum officinarum (Sugarcane) [TaxID: 4547]
Setaria barbata [TaxID: 192628]
Triticum aestivum (Wheat) [TaxID: 4565]
Zea mays (Maize) [TaxID: 4577]
Proteomesi
  • UP000006541 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi201 – 202NE → LI: Complete loss of interaction with RBR1. 1 Publication2
Mutagenesisi201 – 202NE → LK: Complete loss of interaction with RBR1. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002222081 – 272Replication-associated protein AAdd BLAST272

Interactioni

Subunit structurei

Homooligomer. Interacts (via LXCXE domain) with host retinoblastoma-related protein 1 (RBR1), and may thereby deregulate the host cell cycle. Part of the C- and V-complexes which are RepA-Rep-DNA complexes involved in the c-sense and v-sense transcription (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP14990.
SMRiP14990.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni175 – 187OligomerizationBy similarityAdd BLAST13

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi18 – 22RCR-15
Motifi60 – 65RCR-26
Motifi100 – 103RCR-34
Motifi198 – 202LXCXE motif, interaction with host RBR1By similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 6Poly-Ser4

Domaini

There are 3 rolling circle replication (RCR) motifs. RCR-2 may be involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.
The LXCXE motif specifically binds to host RBR1.

Sequence similaritiesi

Belongs to the geminiviridae Rep protein family.Curated

Phylogenomic databases

OrthoDBiVOG090000BW.

Family and domain databases

InterProiView protein in InterPro
IPR001146. Gemini_AL1_MSV.
IPR001191. Gemini_AL1_REP.
IPR022690. Gemini_AL1_REP_cat-dom.
IPR022692. Gemini_AL1_REP_central.
PfamiView protein in Pfam
PF00799. Gemini_AL1. 1 hit.
PF08283. Gemini_AL1_M. 1 hit.
PRINTSiPR00227. GEMCOATAL1.
PR00229. GEMCOATMSVL1.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform RepA (identifier: P14990-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSSSNRQF SHRNANTFLT YPKCPENPEI ACQMIWELVV RWIPKYILCA
60 70 80 90 100
REAHKDGSLH LHALLQTEKP VRISDSRFFD INGFHPNIQS AKSVNRVRDY
110 120 130 140 150
ILKEPLAVFE RGTFIPRKSP FLGKSDSEVK EKKPSKDEIM RDIISHATSK
160 170 180 190 200
AEYLSMIQKE LPFDWSTKLQ YFEYSANKLF PEIQEEFTNP HPPSSPDLLC
210 220 230 240 250
NESINDWLQP NIFQVSPEAY MLLQPTCYTL EDAISDLQWM DSVSSHQMKD
260 270
QESRASTSSA QQEPENLLGP EA
Note: Produced from the unspliced transcript.
Length:272
Mass (Da):31,329
Last modified:April 1, 1990 - v1
Checksum:i36311F3ADDCEED3D
GO
Isoform Rep (identifier: P14989-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P14989.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:355
Mass (Da):41,303
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00514 Genomic DNA. Translation: CAA68569.1.
PIRiS04806.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00514 Genomic DNA. Translation: CAA68569.1.
PIRiS04806.

3D structure databases

ProteinModelPortaliP14990.
SMRiP14990.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OrthoDBiVOG090000BW.

Family and domain databases

InterProiView protein in InterPro
IPR001146. Gemini_AL1_MSV.
IPR001191. Gemini_AL1_REP.
IPR022690. Gemini_AL1_REP_cat-dom.
IPR022692. Gemini_AL1_REP_central.
PfamiView protein in Pfam
PF00799. Gemini_AL1. 1 hit.
PF08283. Gemini_AL1_M. 1 hit.
PRINTSiPR00227. GEMCOATAL1.
PR00229. GEMCOATMSVL1.
ProtoNetiSearch...

Entry informationi

Entry nameiREPA_MSVS
AccessioniPrimary (citable) accession number: P14990
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: March 15, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.