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Protein

Orotidine 5'-phosphate decarboxylase

Gene

ura4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Orotidine 5'-phosphate = UMP + CO2.PROSITE-ProRule annotation

Pathway: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes UMP from orotate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (ura5)
  2. Orotidine 5'-phosphate decarboxylase (ura4)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401SubstrateBy similarity
Active sitei95 – 951Proton donorPROSITE-ProRule annotation
Binding sitei214 – 2141SubstrateBy similarity
Binding sitei233 – 2331SubstrateBy similarity

GO - Molecular functioni

  • orotidine-5'-phosphate decarboxylase activity Source: PomBase

GO - Biological processi

  • 'de novo' pyrimidine nucleobase biosynthetic process Source: PomBase
  • 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  • UMP biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

ReactomeiREACT_327756. Pyrimidine biosynthesis.
UniPathwayiUPA00070; UER00120.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotidine 5'-phosphate decarboxylase (EC:4.1.1.23)
Alternative name(s):
OMP decarboxylase
Short name:
OMPDCase
Short name:
OMPdecase
Uridine 5'-monophosphate synthase
Short name:
UMP synthase
Gene namesi
Name:ura4
ORF Names:SPCC330.05c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC330.05c.
PomBaseiSPCC330.05c. ura4.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Orotidine 5'-phosphate decarboxylasePRO_0000134683Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi275351. 6 interactions.
MINTiMINT-4687324.
STRINGi4896.SPCC330.05c.1.

Structurei

3D structure databases

ProteinModelPortaliP14965.
SMRiP14965. Positions 7-262.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni62 – 643Substrate bindingBy similarity
Regioni93 – 10210Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the OMP decarboxylase family.Curated

Phylogenomic databases

eggNOGiCOG0284.
HOGENOMiHOG000213905.
InParanoidiP14965.
KOiK01591.
OMAiMGQQYRT.
OrthoDBiEOG7CVQ76.
PhylomeDBiP14965.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR018089. OMPdecase_AS.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01740. pyrF. 1 hit.
PROSITEiPS00156. OMPDECASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14965-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDARVFQSYS ARAEGMKNPI AKELLALMEE KQSNLSVAVD LTKKSEILEL
60 70 80 90 100
VDKIGPYVCV IKTHIDVVED FDQDMVEKLV ALGKKHRFLI FEDRKFADIG
110 120 130 140 150
NTVKLQYASG VYKIASWAHI TNCHTVPGEG IIQGLKEVGL PLGRGLLLLA
160 170 180 190 200
EMSSKGSLAT GSYTEKTLEW FEKHTDFCFG FIAGRRFPNL QSDYITMSPG
210 220 230 240 250
IGLDVKGDGL GQQYRTPEEV IVNCGSDIII VGRGVYGAGR NPVVEAKRYR
260
EAGWKAYQQR LSQH
Length:264
Mass (Da):29,362
Last modified:April 1, 1990 - v1
Checksum:iC9B78224E1EE1299
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13976 Genomic DNA. Translation: CAA32157.1.
CU329672 Genomic DNA. Translation: CAA20910.1.
PIRiS08503.
RefSeqiNP_587705.1. NM_001022700.2.

Genome annotation databases

EnsemblFungiiSPCC330.05c.1; SPCC330.05c.1:pep; SPCC330.05c.
GeneIDi2538768.
KEGGispo:SPCC330.05c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13976 Genomic DNA. Translation: CAA32157.1.
CU329672 Genomic DNA. Translation: CAA20910.1.
PIRiS08503.
RefSeqiNP_587705.1. NM_001022700.2.

3D structure databases

ProteinModelPortaliP14965.
SMRiP14965. Positions 7-262.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi275351. 6 interactions.
MINTiMINT-4687324.
STRINGi4896.SPCC330.05c.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPCC330.05c.1; SPCC330.05c.1:pep; SPCC330.05c.
GeneIDi2538768.
KEGGispo:SPCC330.05c.

Organism-specific databases

EuPathDBiFungiDB:SPCC330.05c.
PomBaseiSPCC330.05c. ura4.

Phylogenomic databases

eggNOGiCOG0284.
HOGENOMiHOG000213905.
InParanoidiP14965.
KOiK01591.
OMAiMGQQYRT.
OrthoDBiEOG7CVQ76.
PhylomeDBiP14965.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00120.
ReactomeiREACT_327756. Pyrimidine biosynthesis.

Miscellaneous databases

NextBioi20799952.
PROiP14965.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR018089. OMPdecase_AS.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01740. pyrF. 1 hit.
PROSITEiPS00156. OMPDECASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker."
    Grimm C., Kohli J., Murray J., Maundrell K.
    Mol. Gen. Genet. 215:81-86(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiPYRF_SCHPO
AccessioniPrimary (citable) accession number: P14965
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 24, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.