P14952 (TRPG_CLOTM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component 2
EC=4.1.3.27

Alternative name(s):
Anthranilate synthase component II
Glutamine amido-transferase

Gene names

Name:

trpG

Organism

Clostridium thermocellum

Taxonomic identifier

1515 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	91 AA.
Sequence status	Fragment.
Protein existence	Predicted

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Domain	Glutamine amidotransferase
Molecular function	Lyase
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›91	›91	Anthranilate synthase component 2		PRO_0000056875
Regions
	Domain	4 – ›91	›88	Glutamine amidotransferase type-1
Sites
	Active site	82	1	By similarity
Experimental info
	Non-terminal residue	91	1

Sequences

	Sequence		Length	Mass (Da)
	P14952 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: E5718595500BF1AF	FASTA	91	10,272
	10 20 30 40 50 60 MIENILIIDN YDSFTYNLYQ YVGEISPNIE VYRNDKITLE KIEEMNPTHI IISPGPGFPK 70 80 90 DAGICIEAIR KFGRYIPILG VCLGHQAIGE A

References

[1]	"Molecular cloning and the nucleotide sequence of the Clostridium thermocellum trpE gene." Sato S., Nakada Y., Hon-Nami K., Yasui K., Shiratsuchi A. J. Biochem. 105:362-366(1989) [PubMed: 2732211] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00399 Genomic DNA. Translation: BAA00301.1.
PIR	PT0065.
3D structure databases
ProteinModelPortal	P14952.
SMR	P14952. Positions 4-91.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017926. GATASE_1. [Graphical view]
Pfam	PF00117. GATase. 1 hit. [Graphical view]
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPG_CLOTM

Accession

Primary (citable) accession number: P14952

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program