P14942 (GSTA4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase alpha-4
EC=2.5.1.18

Alternative name(s):
GST 8-8
GST A4-4
GST K
Glutathione S-transferase Yk
Short name=GST Yk

Gene names

Name:

Gsta4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	xenobiotic metabolic process Inferred from direct assay. Source: RGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	drug binding Inferred from direct assay. Source: RGD glutathione binding Inferred from direct assay. Source: RGD glutathione transferase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Glutathione S-transferase alpha-4		PRO_0000185795
Regions
	Domain	3 – 83	81	GST N-terminal
	Domain	85 – 208	124	GST C-terminal
	Region	54 – 55	2	Glutathione binding By similarity
	Region	67 – 68	2	Glutathione binding By similarity
Sites
	Binding site	9	1	Glutathione By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.1
Experimental info
	Sequence conflict	18	1	S → V AA sequence Ref.1
	Sequence conflict	48	1	G → D AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P14942 [UniParc]. Last modified December 1, 1992. Version 2. Checksum: E0E42852DBA37E58	FASTA	222	25,510
	10 20 30 40 50 60 MEVKPKLYYF QGRGRMESIR WLLATAGVEF EEEFLETREQ YEKLQKDGCL LFGQVPLVEI 70 80 90 100 110 120 DGMLLTQTRA ILSYLAAKYN LYGKDLKERV RIDMYADGTQ DLMMMIIGAP FKAPQEKEES 130 140 150 160 170 180 LALAVKRAKN RYFPVFEKIL KDHGEAFLVG NQLSWADIQL LEAILMVEEV SAPVLSDFPL 190 200 210 220 LQAFKTRISN IPTIKKFLQP GSQRKPPPDG HYVDVVRTVL KF

References

[1]	"Cytosolic glutathione transferases from rat liver. Primary structure of class alpha glutathione transferase 8-8 and characterization of low-abundance class Mu glutathione transferases." Alin P., Jensson H., Cederlund E., Joernvall H., Mannervik B. Biochem. J. 261:531-539(1989) [PubMed: 2775231] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Liver.
[2]	"Cloning and heterologous expression of cDNA encoding class alpha rat glutathione transferase 8-8, an enzyme with high catalytic activity towards genotoxic alpha,beta-unsaturated carbonyl compounds." Stenberg G., Ridderstroem M., Engstroem A., Pemble S.E., Mannervik B. Biochem. J. 284:313-319(1992) [PubMed: 1599415] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Hepatoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62660 mRNA. Translation: CAB46530.1.
IPI	IPI00210542.
PIR	XURT8C. S23433.
RefSeq	NP_001100310.1. NM_001106840.1.
UniGene	Rn.57528.
3D structure databases
ProteinModelPortal	P14942.
SMR	P14942. Positions 4-222.
ModBase	Search...
Protein-protein interaction databases
STRING	P14942.
Proteomic databases
PRIDE	P14942.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000012348; ENSRNOP00000012346; ENSRNOG00000030449.
GeneID	300850.
KEGG	rno:300850.
NMPDR	fig|10116.3.peg.28966.
Organism-specific databases
CTD	2941.
RGD	1309970. Gsta4.
Phylogenomic databases
eggNOG	roNOG04634.
GeneTree	ENSGT00550000074445.
HOVERGEN	HBG053749.
InParanoid	P14942.
OMA	MYVEGIS.
OrthoDB	EOG4N5VXR.
PhylomeDB	P14942.
Gene expression databases
ArrayExpress	P14942.
Genevestigator	P14942.
GermOnline	ENSRNOG00000030449. Rattus norvegicus.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR003080. GST_alpha. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KO	K00799.
PANTHER	PTHR11571:SF4. GST_alpha. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01266. GSTRNSFRASEA.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	647640.

Entry information

Entry name

GSTA4_RAT

Accession

Primary (citable) accession number: P14942

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	December 1, 1992
Last modified:	November 16, 2011
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program