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Protein

Glutathione S-transferase alpha-4

Gene

Gsta4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91GlutathioneBy similarity

GO - Molecular functioni

  1. drug binding Source: RGD
  2. glutathione binding Source: RGD
  3. glutathione transferase activity Source: RGD

GO - Biological processi

  1. cellular response to lithium ion Source: RGD
  2. response to herbicide Source: RGD
  3. response to nicotine Source: RGD
  4. response to zinc ion Source: RGD
  5. xenobiotic metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase alpha-4 (EC:2.5.1.18)
Alternative name(s):
GST 8-8
GST A4-4
GST K
Glutathione S-transferase Yk
Short name:
GST Yk
Gene namesi
Name:Gsta4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi1309970. Gsta4.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 222222Glutathione S-transferase alpha-4PRO_0000185795Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP14942.
PRIDEiP14942.

Expressioni

Gene expression databases

ExpressionAtlasiP14942. baseline.
GenevestigatoriP14942.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP14942.
SMRiP14942. Positions 4-222.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 8381GST N-terminalAdd
BLAST
Domaini85 – 208124GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni54 – 552Glutathione bindingBy similarity
Regioni67 – 682Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiNOG266414.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP14942.
KOiK00799.
OMAiMGMIMYL.
OrthoDBiEOG79CZ0K.
PhylomeDBiP14942.
TreeFamiTF105321.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14942-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVKPKLYYF QGRGRMESIR WLLATAGVEF EEEFLETREQ YEKLQKDGCL
60 70 80 90 100
LFGQVPLVEI DGMLLTQTRA ILSYLAAKYN LYGKDLKERV RIDMYADGTQ
110 120 130 140 150
DLMMMIIGAP FKAPQEKEES LALAVKRAKN RYFPVFEKIL KDHGEAFLVG
160 170 180 190 200
NQLSWADIQL LEAILMVEEV SAPVLSDFPL LQAFKTRISN IPTIKKFLQP
210 220
GSQRKPPPDG HYVDVVRTVL KF
Length:222
Mass (Da):25,510
Last modified:December 1, 1992 - v2
Checksum:iE0E42852DBA37E58
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181S → V AA sequence (PubMed:2775231).Curated
Sequence conflicti48 – 481G → D AA sequence (PubMed:2775231).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62660 mRNA. Translation: CAB46530.1.
PIRiS23433. XURT8C.
RefSeqiNP_001100310.1. NM_001106840.1.
UniGeneiRn.57528.

Genome annotation databases

EnsembliENSRNOT00000012348; ENSRNOP00000012346; ENSRNOG00000030449.
GeneIDi300850.
KEGGirno:300850.
UCSCiRGD:1309970. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62660 mRNA. Translation: CAB46530.1.
PIRiS23433. XURT8C.
RefSeqiNP_001100310.1. NM_001106840.1.
UniGeneiRn.57528.

3D structure databases

ProteinModelPortaliP14942.
SMRiP14942. Positions 4-222.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiP14942.
PRIDEiP14942.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012348; ENSRNOP00000012346; ENSRNOG00000030449.
GeneIDi300850.
KEGGirno:300850.
UCSCiRGD:1309970. rat.

Organism-specific databases

CTDi2941.
RGDi1309970. Gsta4.

Phylogenomic databases

eggNOGiNOG266414.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP14942.
KOiK00799.
OMAiMGMIMYL.
OrthoDBiEOG79CZ0K.
PhylomeDBiP14942.
TreeFamiTF105321.

Miscellaneous databases

NextBioi647640.

Gene expression databases

ExpressionAtlasiP14942. baseline.
GenevestigatoriP14942.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cytosolic glutathione transferases from rat liver. Primary structure of class alpha glutathione transferase 8-8 and characterization of low-abundance class Mu glutathione transferases."
    Alin P., Jensson H., Cederlund E., Joernvall H., Mannervik B.
    Biochem. J. 261:531-539(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, ACETYLATION AT MET-1.
    Tissue: Liver.
  2. "Cloning and heterologous expression of cDNA encoding class alpha rat glutathione transferase 8-8, an enzyme with high catalytic activity towards genotoxic alpha,beta-unsaturated carbonyl compounds."
    Stenberg G., Ridderstroem M., Engstroem A., Pemble S.E., Mannervik B.
    Biochem. J. 284:313-319(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Hepatoma.

Entry informationi

Entry nameiGSTA4_RAT
AccessioniPrimary (citable) accession number: P14942
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: December 1, 1992
Last modified: January 7, 2015
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.