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Protein

Glutathione S-transferase alpha-4

Gene

Gsta4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9GlutathioneBy similarity1

GO - Molecular functioni

  • drug binding Source: RGD
  • glutathione binding Source: RGD
  • glutathione transferase activity Source: RGD

GO - Biological processi

  • cellular response to lithium ion Source: RGD
  • response to herbicide Source: RGD
  • response to nicotine Source: RGD
  • response to zinc ion Source: RGD
  • xenobiotic metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase alpha-4 (EC:2.5.1.18)
Alternative name(s):
GST 8-8
GST A4-4
GST K
Glutathione S-transferase Yk
Short name:
GST Yk
Gene namesi
Name:Gsta4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1309970. Gsta4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001857951 – 222Glutathione S-transferase alpha-4Add BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP14942.
PRIDEiP14942.

PTM databases

iPTMnetiP14942.
PhosphoSitePlusiP14942.

Expressioni

Gene expression databases

BgeeiENSRNOG00000030449.
ExpressionAtlasiP14942. baseline and differential.
GenevisibleiP14942. RN.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi256738. 1 interactor.
STRINGi10116.ENSRNOP00000012346.

Structurei

3D structure databases

ProteinModelPortaliP14942.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 83GST N-terminalAdd BLAST81
Domaini85 – 208GST C-terminalAdd BLAST124

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 55Glutathione bindingBy similarity2
Regioni67 – 68Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP14942.
KOiK00799.
OMAiMGMIMYL.
OrthoDBiEOG091G0O3D.
PhylomeDBiP14942.
TreeFamiTF105321.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14942-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVKPKLYYF QGRGRMESIR WLLATAGVEF EEEFLETREQ YEKLQKDGCL
60 70 80 90 100
LFGQVPLVEI DGMLLTQTRA ILSYLAAKYN LYGKDLKERV RIDMYADGTQ
110 120 130 140 150
DLMMMIIGAP FKAPQEKEES LALAVKRAKN RYFPVFEKIL KDHGEAFLVG
160 170 180 190 200
NQLSWADIQL LEAILMVEEV SAPVLSDFPL LQAFKTRISN IPTIKKFLQP
210 220
GSQRKPPPDG HYVDVVRTVL KF
Length:222
Mass (Da):25,510
Last modified:December 1, 1992 - v2
Checksum:iE0E42852DBA37E58
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18S → V AA sequence (PubMed:2775231).Curated1
Sequence conflicti48G → D AA sequence (PubMed:2775231).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62660 mRNA. Translation: CAB46530.1.
PIRiS23433. XURT8C.
RefSeqiNP_001100310.1. NM_001106840.1.
UniGeneiRn.57528.

Genome annotation databases

EnsembliENSRNOT00000012348; ENSRNOP00000012346; ENSRNOG00000030449.
ENSRNOT00000090146; ENSRNOP00000075572; ENSRNOG00000030449.
GeneIDi300850.
KEGGirno:300850.
UCSCiRGD:1309970. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62660 mRNA. Translation: CAB46530.1.
PIRiS23433. XURT8C.
RefSeqiNP_001100310.1. NM_001106840.1.
UniGeneiRn.57528.

3D structure databases

ProteinModelPortaliP14942.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi256738. 1 interactor.
STRINGi10116.ENSRNOP00000012346.

PTM databases

iPTMnetiP14942.
PhosphoSitePlusiP14942.

Proteomic databases

PaxDbiP14942.
PRIDEiP14942.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012348; ENSRNOP00000012346; ENSRNOG00000030449.
ENSRNOT00000090146; ENSRNOP00000075572; ENSRNOG00000030449.
GeneIDi300850.
KEGGirno:300850.
UCSCiRGD:1309970. rat.

Organism-specific databases

CTDi2941.
RGDi1309970. Gsta4.

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP14942.
KOiK00799.
OMAiMGMIMYL.
OrthoDBiEOG091G0O3D.
PhylomeDBiP14942.
TreeFamiTF105321.

Miscellaneous databases

PROiP14942.

Gene expression databases

BgeeiENSRNOG00000030449.
ExpressionAtlasiP14942. baseline and differential.
GenevisibleiP14942. RN.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTA4_RAT
AccessioniPrimary (citable) accession number: P14942
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.