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P14942

- GSTA4_RAT

UniProt

P14942 - GSTA4_RAT

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Protein

Glutathione S-transferase alpha-4

Gene
Gsta4
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91Glutathione By similarity

GO - Molecular functioni

  1. drug binding Source: RGD
  2. glutathione binding Source: RGD
  3. glutathione transferase activity Source: RGD

GO - Biological processi

  1. cellular response to lithium ion Source: RGD
  2. response to herbicide Source: RGD
  3. response to nicotine Source: RGD
  4. response to zinc ion Source: RGD
  5. xenobiotic metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase alpha-4 (EC:2.5.1.18)
Alternative name(s):
GST 8-8
GST A4-4
GST K
Glutathione S-transferase Yk
Short name:
GST Yk
Gene namesi
Name:Gsta4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi1309970. Gsta4.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 222222Glutathione S-transferase alpha-4PRO_0000185795Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP14942.
PRIDEiP14942.

Expressioni

Gene expression databases

GenevestigatoriP14942.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP14942.
SMRiP14942. Positions 4-222.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 8381GST N-terminalAdd
BLAST
Domaini85 – 208124GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni54 – 552Glutathione binding By similarity
Regioni67 – 682Glutathione binding By similarity

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.

Phylogenomic databases

eggNOGiNOG266414.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP14942.
KOiK00799.
OMAiPDDKYVE.
OrthoDBiEOG79CZ0K.
PhylomeDBiP14942.
TreeFamiTF105321.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14942-1 [UniParc]FASTAAdd to Basket

« Hide

MEVKPKLYYF QGRGRMESIR WLLATAGVEF EEEFLETREQ YEKLQKDGCL    50
LFGQVPLVEI DGMLLTQTRA ILSYLAAKYN LYGKDLKERV RIDMYADGTQ 100
DLMMMIIGAP FKAPQEKEES LALAVKRAKN RYFPVFEKIL KDHGEAFLVG 150
NQLSWADIQL LEAILMVEEV SAPVLSDFPL LQAFKTRISN IPTIKKFLQP 200
GSQRKPPPDG HYVDVVRTVL KF 222
Length:222
Mass (Da):25,510
Last modified:December 1, 1992 - v2
Checksum:iE0E42852DBA37E58
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181S → V AA sequence 1 Publication
Sequence conflicti48 – 481G → D AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62660 mRNA. Translation: CAB46530.1.
PIRiS23433. XURT8C.
RefSeqiNP_001100310.1. NM_001106840.1.
UniGeneiRn.57528.

Genome annotation databases

EnsembliENSRNOT00000012348; ENSRNOP00000012346; ENSRNOG00000030449.
GeneIDi300850.
KEGGirno:300850.
UCSCiRGD:1309970. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62660 mRNA. Translation: CAB46530.1 .
PIRi S23433. XURT8C.
RefSeqi NP_001100310.1. NM_001106840.1.
UniGenei Rn.57528.

3D structure databases

ProteinModelPortali P14942.
SMRi P14942. Positions 4-222.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P14942.
PRIDEi P14942.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000012348 ; ENSRNOP00000012346 ; ENSRNOG00000030449 .
GeneIDi 300850.
KEGGi rno:300850.
UCSCi RGD:1309970. rat.

Organism-specific databases

CTDi 2941.
RGDi 1309970. Gsta4.

Phylogenomic databases

eggNOGi NOG266414.
GeneTreei ENSGT00670000097856.
HOGENOMi HOG000115734.
HOVERGENi HBG053749.
InParanoidi P14942.
KOi K00799.
OMAi PDDKYVE.
OrthoDBi EOG79CZ0K.
PhylomeDBi P14942.
TreeFami TF105321.

Miscellaneous databases

NextBioi 647640.

Gene expression databases

Genevestigatori P14942.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view ]
PRINTSi PR01266. GSTRNSFRASEA.
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cytosolic glutathione transferases from rat liver. Primary structure of class alpha glutathione transferase 8-8 and characterization of low-abundance class Mu glutathione transferases."
    Alin P., Jensson H., Cederlund E., Joernvall H., Mannervik B.
    Biochem. J. 261:531-539(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, ACETYLATION AT MET-1.
    Tissue: Liver.
  2. "Cloning and heterologous expression of cDNA encoding class alpha rat glutathione transferase 8-8, an enzyme with high catalytic activity towards genotoxic alpha,beta-unsaturated carbonyl compounds."
    Stenberg G., Ridderstroem M., Engstroem A., Pemble S.E., Mannervik B.
    Biochem. J. 284:313-319(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Hepatoma.

Entry informationi

Entry nameiGSTA4_RAT
AccessioniPrimary (citable) accession number: P14942
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: December 1, 1992
Last modified: June 11, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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