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P14942

- GSTA4_RAT

UniProt

P14942 - GSTA4_RAT

Protein

Glutathione S-transferase alpha-4

Gene

Gsta4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei9 – 91GlutathioneBy similarity

    GO - Molecular functioni

    1. drug binding Source: RGD
    2. glutathione binding Source: RGD
    3. glutathione transferase activity Source: RGD

    GO - Biological processi

    1. cellular response to lithium ion Source: RGD
    2. response to herbicide Source: RGD
    3. response to nicotine Source: RGD
    4. response to zinc ion Source: RGD
    5. xenobiotic metabolic process Source: RGD

    Keywords - Molecular functioni

    Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase alpha-4 (EC:2.5.1.18)
    Alternative name(s):
    GST 8-8
    GST A4-4
    GST K
    Glutathione S-transferase Yk
    Short name:
    GST Yk
    Gene namesi
    Name:Gsta4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi1309970. Gsta4.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. mitochondrion Source: RGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 222222Glutathione S-transferase alpha-4PRO_0000185795Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP14942.
    PRIDEiP14942.

    Expressioni

    Gene expression databases

    GenevestigatoriP14942.

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP14942.
    SMRiP14942. Positions 4-222.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 8381GST N-terminalAdd
    BLAST
    Domaini85 – 208124GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni54 – 552Glutathione bindingBy similarity
    Regioni67 – 682Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Alpha family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiNOG266414.
    GeneTreeiENSGT00670000097856.
    HOGENOMiHOG000115734.
    HOVERGENiHBG053749.
    InParanoidiP14942.
    KOiK00799.
    OMAiPDDKYVE.
    OrthoDBiEOG79CZ0K.
    PhylomeDBiP14942.
    TreeFamiTF105321.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR003080. GST_alpha.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    PRINTSiPR01266. GSTRNSFRASEA.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14942-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVKPKLYYF QGRGRMESIR WLLATAGVEF EEEFLETREQ YEKLQKDGCL    50
    LFGQVPLVEI DGMLLTQTRA ILSYLAAKYN LYGKDLKERV RIDMYADGTQ 100
    DLMMMIIGAP FKAPQEKEES LALAVKRAKN RYFPVFEKIL KDHGEAFLVG 150
    NQLSWADIQL LEAILMVEEV SAPVLSDFPL LQAFKTRISN IPTIKKFLQP 200
    GSQRKPPPDG HYVDVVRTVL KF 222
    Length:222
    Mass (Da):25,510
    Last modified:December 1, 1992 - v2
    Checksum:iE0E42852DBA37E58
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti18 – 181S → V AA sequence (PubMed:2775231)Curated
    Sequence conflicti48 – 481G → D AA sequence (PubMed:2775231)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62660 mRNA. Translation: CAB46530.1.
    PIRiS23433. XURT8C.
    RefSeqiNP_001100310.1. NM_001106840.1.
    UniGeneiRn.57528.

    Genome annotation databases

    EnsembliENSRNOT00000012348; ENSRNOP00000012346; ENSRNOG00000030449.
    GeneIDi300850.
    KEGGirno:300850.
    UCSCiRGD:1309970. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62660 mRNA. Translation: CAB46530.1 .
    PIRi S23433. XURT8C.
    RefSeqi NP_001100310.1. NM_001106840.1.
    UniGenei Rn.57528.

    3D structure databases

    ProteinModelPortali P14942.
    SMRi P14942. Positions 4-222.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P14942.
    PRIDEi P14942.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000012348 ; ENSRNOP00000012346 ; ENSRNOG00000030449 .
    GeneIDi 300850.
    KEGGi rno:300850.
    UCSCi RGD:1309970. rat.

    Organism-specific databases

    CTDi 2941.
    RGDi 1309970. Gsta4.

    Phylogenomic databases

    eggNOGi NOG266414.
    GeneTreei ENSGT00670000097856.
    HOGENOMi HOG000115734.
    HOVERGENi HBG053749.
    InParanoidi P14942.
    KOi K00799.
    OMAi PDDKYVE.
    OrthoDBi EOG79CZ0K.
    PhylomeDBi P14942.
    TreeFami TF105321.

    Miscellaneous databases

    NextBioi 647640.

    Gene expression databases

    Genevestigatori P14942.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR003080. GST_alpha.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01266. GSTRNSFRASEA.
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cytosolic glutathione transferases from rat liver. Primary structure of class alpha glutathione transferase 8-8 and characterization of low-abundance class Mu glutathione transferases."
      Alin P., Jensson H., Cederlund E., Joernvall H., Mannervik B.
      Biochem. J. 261:531-539(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, ACETYLATION AT MET-1.
      Tissue: Liver.
    2. "Cloning and heterologous expression of cDNA encoding class alpha rat glutathione transferase 8-8, an enzyme with high catalytic activity towards genotoxic alpha,beta-unsaturated carbonyl compounds."
      Stenberg G., Ridderstroem M., Engstroem A., Pemble S.E., Mannervik B.
      Biochem. J. 284:313-319(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Hepatoma.

    Entry informationi

    Entry nameiGSTA4_RAT
    AccessioniPrimary (citable) accession number: P14942
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3