P14941 (ADH_THEBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADP-dependent alcohol dehydrogenase
EC=1.1.1.2

Gene names

Name:

adh

Organism

Thermoanaerobacter brockii (Thermoanaerobium brockii)

Taxonomic identifier

29323 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Thermoanaerobacter

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	An alcohol + NADP+ = an aldehyde + NADPH.
Cofactor	Binds 1 zinc ion per subunit.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	Metal-binding NADP Zinc
Molecular function	Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	alcohol dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 352

352

NADP-dependent alcohol dehydrogenase

PRO_0000160749

Sites

Metal binding

37

1

Zinc; catalytic

Metal binding

59

1

Zinc; catalytic

Metal binding

150

1

Zinc; catalytic

Secondary structure

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352

Helix Strand Turn

Details...

Beta strand

2 – 8

7

Beta strand

11 – 16

6

Beta strand

27 – 35

9

Helix

38 – 45

8

Beta strand

53 – 56

4

Beta strand

60 – 68

9

Beta strand

80 – 83

4

Beta strand

90 – 92

3

Helix

93 – 96

4

Helix

100 – 102

3

Turn

106 – 109

4

Turn

112 – 114

3

Beta strand

121 – 128

8

Helix

129 – 132

4

Helix

142 – 145

4

Turn

146 – 150

5

Helix

151 – 161

11

Beta strand

170 – 173

4

Helix

177 – 187

11

Turn

188 – 190

3

Beta strand

192 – 197

6

Helix

201 – 210

10

Beta strand

213 – 216

4

Helix

218 – 220

3

Helix

223 – 230

8

Turn

231 – 233

3

Beta strand

236 – 241

6

Helix

248 – 255

8

Beta strand

256 – 264

9

Beta strand

270 – 272

3

Turn

278 – 285

8

Beta strand

289 – 293

5

Helix

299 – 310

12

Helix

316 – 319

4

Beta strand

320 – 326

7

Helix

328 – 338

11

Beta strand

345 – 350

6

Sequences

	Sequence		Length	Mass (Da)
	P14941 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: E124A5351AD55185	FASTA	352	37,647
	10 20 30 40 50 60 MKGFAMLSIG KVGWIEKEKP APGPFDAIVR PLAVAPCTSD IHTVFEGAIG ERHNMILGHE 70 80 90 100 110 120 AVGEVVEVGS EVKDFKPGDR VVVPAITPDW RTSEVQRGYH QHSGGMLAGW KFSNVKDGVF 130 140 150 160 170 180 GEFFHVNDAD MNLAHLPKEI PLEAAVMIPD MMTTGFHGAE LADIELGATV AVLGIGPVGL 190 200 210 220 230 240 MAVAGAKLRG AGRIIAVGSR PVCVDAAKYY GATDIVNYKD GPIESQIMNL TEGKGVDAAI 250 260 270 280 290 300 IAGGNADIMA TAVKIVKPGG TIANVNYFGE GEVLPVPRLE WGCGMAHKTI KGGLCPGGRL 310 320 330 340 350 RMERLIDLVF YKRVDPSKLV THVFRGFDNI EKAFMLMKDK PKDLIKPVVI LA

References

[1]	"Amino acid sequence of alcohol dehydrogenase from the thermophilic bacterium Thermoanaerobium brockii." Peretz M., Burstein Y. Biochemistry 28:6549-6555(1989) [PubMed: 2790012] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: ATCC 53556 / HTD4 / DSM 1457.
[2]	Burstein Y. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 53556 / HTD4 / DSM 1457.
[3]	"NADP-dependent bacterial alcohol dehydrogenases: crystal structure, cofactor-binding and cofactor specificity of the ADHs of Clostridium beijerinckii and Thermoanaerobacter brockii." Korkhin Y., Kalb A.J., Peretz M., Bogin O., Burstein Y., Frolow F. J. Mol. Biol. 278:967-981(1998) [PubMed: 9836873] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X64841 Genomic DNA. Translation: CAA46053.1.

PIR

A32973.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BXZ	X-ray	2.99	A/B/C/D	1-352	[»]
1YKF	X-ray	2.50	A/B/C/D	1-352	[»]
2NVB	X-ray	2.80	A/B/C/D	1-352	[»]
3FPC	X-ray	1.40	A/B/C/D	1-352	[»]
3FPL	X-ray	1.90	A	153-295	[»]
3FSR	X-ray	2.20	A/B/C/D	1-352	[»]
3FTN	X-ray	2.19	A/B/C/D	1-352	[»]

ProteinModelPortal

P14941.

SMR

P14941. Positions 1-352.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR11695. ADH_Sf_Zn. 1 hit.

Pfam

PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]

SUPFAM

SSF50129. GroES_like. 1 hit.

PROSITE

PS00059. ADH_ZINC. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ADH_THEBR

Accession

Primary (citable) accession number: P14941

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program