Reviewed,
UniProtKB/Swiss-Prot P14922 (CYC8_YEAST)
Last modified
June 16, 2009.
Version 108.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: General transcriptional corepressor CYC8 Alternative name(s): Glucose repression mediator protein CYC8 | ||||||||
| Gene names |
| ||||||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 966 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Acts as component of the CYC8-TUP1 corepressor complex which is involved in the repression of many genes in a wide variety of physiological processes including heme-regulated and catabolite repressed genes. May also be involved in the derepression of at least some target genes. The complex is recruited to target genes by interaction with DNA-bound transcriptional repressors, like MATALPHA2, MIG1, RFX1 and SKO1. The complex recruits histone deacetylases to produce a repressive chromatin structure, interacts with hypoacetylated N-terminal tails of histones H3 and H4 that have been programmed for repression by the action of histone deacetylases and interferes directly with the transcriptional machinery by associating with the RNA polymerase II mediator complex. Ref.10 Ref.11 Ref.13 Ref.17 Ref.18 |
| Subunit structure | Associates with TUP1 to form the CYC8-TUP1 (or TUP1-SSN6) corepressor complex that is composed of 4 copies of TUP1 and one copy of CYC8. Interacts with MATALPHA2, CTI6, MIG1, TUP1, SUT1, RFX1, PGD1, HOS1, HOS2 AND RPD3. Ref.10 Ref.11 Ref.6 Ref.7 Ref.8 Ref.9 Ref.12 Ref.14 Ref.15 Ref.16 |
| Subcellular location | |
| Miscellaneous | Present with 3890 molecules/cell in log phase SD medium. Ref.19 |
| Sequence similarities | Belongs to the CYC8/SSN6 family. Contains 10 TPR repeats. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Transcription Transcription regulation |
| Cellular component | Nucleus |
| Domain | Repeat TPR repeat |
| Molecular function | Repressor |
| PTM | Phosphoprotein |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | chromatin remodeling Inferred from mutant phenotype. Source: SGD negative regulation of transcriptionInferred from direct assay. Source: SGD nucleosome positioningInferred from direct assay. Source: SGD regulation of transcription, DNA-dependentInferred from electronic annotation. Source: UniProtKB-KW transcriptionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | transcriptional repressor complex Inferred from direct assay. Source: SGD |
| Molecular function | general transcriptional repressor activity Inferred from direct assay. Source: SGD transcription coactivator activityInferred from direct assay. Source: SGD |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| HOS1 | Q12214 | 1 | EBI-18215,EBI-8471 | |
| HOS2 | P53096 | 4 | EBI-18215,EBI-8475 | |
| PGD1 | P40356 | 1 | EBI-18215,EBI-13268 | |
| RFX1 | P48743 | 1 | EBI-18215,EBI-15036 | |
| RPD3 | P32561 | 4 | EBI-18215,EBI-15864 | |
| TAF2 | P23255 | 1 | EBI-18215,EBI-18862 | |
| TUP1 | P16649 | 4 | EBI-18215,EBI-19654 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 966 | 966 | General transcriptional corepressor CYC8 | PRO_0000106327 | |||||
Regions | |||||||||
| Repeat | 46 – 79 | 34 | TPR 1 | ||||||
| Repeat | 80 – 113 | 34 | TPR 2 | ||||||
| Repeat | 114 – 147 | 34 | TPR 3 | ||||||
| Repeat | 150 – 183 | 34 | TPR 4 | ||||||
| Repeat | 187 – 220 | 34 | TPR 5 | ||||||
| Repeat | 224 – 257 | 34 | TPR 6 | ||||||
| Repeat | 262 – 295 | 34 | TPR 7 | ||||||
| Repeat | 296 – 329 | 34 | TPR 8 | ||||||
| Repeat | 330 – 363 | 34 | TPR 9 | ||||||
| Repeat | 364 – 398 | 35 | TPR 10 | ||||||
| Region | 493 – 556 | 64 | 30 X 2 AA tandem repeats of Q-A | ||||||
| Compositional bias | 15 – 30 | 16 | Poly-Gln | ||||||
| Compositional bias | 557 – 587 | 31 | Poly-Gln | ||||||
Amino acid modifications | |||||||||
| Modified residue | 429 | 1 | Phosphoserine Ref.24 | ||||||
| Modified residue | 475 | 1 | Phosphothreonine Ref.24 | ||||||
| Modified residue | 710 | 1 | Phosphoserine Ref.24 Ref.22 | ||||||
| Modified residue | 741 | 1 | Phosphoserine Ref.24 Ref.23 | ||||||
| Modified residue | 755 | 1 | Phosphoserine Ref.24 | ||||||
| Modified residue | 768 | 1 | Phosphoserine Ref.24 | ||||||
| Modified residue | 779 | 1 | Phosphothreonine Ref.24 | ||||||
| Modified residue | 780 | 1 | Phosphoserine; by ATM or ATR Ref.24 Ref.23 | ||||||
| Modified residue | 793 | 1 | Phosphoserine Ref.24 | ||||||
| Modified residue | 805 | 1 | Phosphoserine Ref.24 Ref.20 | ||||||
| Modified residue | 815 | 1 | Phosphoserine Ref.24 Ref.23 Ref.21 | ||||||
| Modified residue | 817 | 1 | Phosphoserine Ref.24 Ref.21 | ||||||
| Modified residue | 866 | 1 | Phosphoserine Ref.24 | ||||||
| Modified residue | 939 | 1 | Phosphoserine Ref.24 | ||||||
| Modified residue | 943 | 1 | Phosphoserine Ref.24 Ref.21 | ||||||
Experimental info | |||||||||
| Sequence conflict | 547 | 1 | K → Q in CAA46973. Ref.3 | ||||||
| Sequence conflict | 547 | 1 | K → Q in CAA55615. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and characterization of the CYC8 gene mediating glucose repression in yeast." Trumbly R.J. Gene 73:97-111(1988) [PubMed: 2854095] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Molecular analysis of SSN6, a gene functionally related to the SNF1 protein kinase of Saccharomyces cerevisiae." Schultz J., Carlson M. Mol. Cell. Biol. 7:3637-3645(1987) [PubMed: 3316983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Molecular analysis of yeast chromosome II between CMD1 and LYS2: the excision repair gene RAD16 located in this region belongs to a novel group of double-finger proteins." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 8:397-408(1992) [PubMed: 1626431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [4] | "Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K.EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [5] | "A repeating amino acid motif in CDC23 defines a family of proteins and a new relationship among genes required for mitosis and RNA synthesis." Sikorski R.S., Boguski M.S., Goebl M., Hieter P.A. Cell 60:307-317(1990) [PubMed: 2404612] [Abstract] Cited for: DOMAINS TPR REPEATS. |
| [6] | "The tetratricopeptide repeats of Ssn6 interact with the homeo domain of alpha 2." Smith R.L., Redd M.J., Johnson A.D. Genes Dev. 9:2903-2910(1995) [PubMed: 7498787] [Abstract] Cited for: INTERACTION WITH MATALPHA2. |
| [7] | "Repression by SSN6-TUP1 is directed by MIG1, a repressor/activator protein." Treitel M.A., Carlson M. Proc. Natl. Acad. Sci. U.S.A. 92:3132-3136(1995) [PubMed: 7724528] [Abstract] Cited for: INTERACTION WITH MIG1. |
| [8] | "The Cyc8 (Ssn6)-Tup1 corepressor complex is composed of one Cyc8 and four Tup1 subunits." Varanasi U.S., Klis M., Mikesell P.B., Trumbly R.J. Mol. Cell. Biol. 16:6707-6714(1996) [PubMed: 8943325] [Abstract] Cited for: SUBUNIT. |
| [9] | "The DNA replication and damage checkpoint pathways induce transcription by inhibition of the Crt1 repressor." Huang M., Zhou Z., Elledge S.J. Cell 94:595-605(1998) [PubMed: 9741624] [Abstract] Cited for: INTERACTION WITH RFX1. |
| [10] | "Ssn6-Tup1 interacts with class I histone deacetylases required for repression." Watson A.D., Edmondson D.G., Bone J.R., Mukai Y., Yu Y., Du W., Stillman D.J., Roth S.Y. Genes Dev. 14:2737-2744(2000) [PubMed: 11069890] [Abstract] Cited for: INTERACTION WITH TUP1; HOS2 AND RPD3, FUNCTION OF THE CYC8-TUP1 COMPLEX. |
| [11] | "Hrs1/Med3 is a Cyc8-Tup1 corepressor target in the RNA polymerase II holoenzyme." Papamichos-Chronakis M., Conlan R.S., Gounalaki N., Copf T., Tzamarias D. J. Biol. Chem. 275:8397-8403(2000) [PubMed: 10722672] [Abstract] Cited for: INTERACTION WITH PGD1, FUNCTION OF THE CYC8-TUP1 COMPLEX. |
| [12] | "A sequence resembling a peroxisomal targeting sequence directs the interaction between the tetratricopeptide repeats of Ssn6 and the homeodomain of alpha 2." Smith R.L., Johnson A.D. Proc. Natl. Acad. Sci. U.S.A. 97:3901-3906(2000) [PubMed: 10759558] [Abstract] Cited for: INTERACTION WITH MATALPHA2. |
| [13] | "Regulation of the Sko1 transcriptional repressor by the Hog1 MAP kinase in response to osmotic stress." Proft M., Pascual-Ahuir A., de Nadal E., Arino J., Serrano R., Posas F. EMBO J. 20:1123-1133(2001) [PubMed: 11230135] [Abstract] Cited for: FUNCTION OF THE CYC8-TUP1 COMPLEX. |
| [14] | "SUT1p interaction with Cyc8p(Ssn6p) relieves hypoxic genes from Cyc8p-Tup1p repression in Saccharomyces cerevisiae." Regnacq M., Alimardani P., El Moudni B., Berges T. Mol. Microbiol. 40:1085-1096(2001) [PubMed: 11401714] [Abstract] Cited for: INTERACTION WITH SUT1. |
| [15] | "Cti6, a PHD domain protein, bridges the Cyc8-Tup1 corepressor and the SAGA coactivator to overcome repression at GAL1." Papamichos-Chronakis M., Petrakis T., Ktistaki E., Topalidou I., Tzamarias D. Mol. Cell 9:1297-1305(2002) [PubMed: 12086626] [Abstract] Cited for: INTERACTION WITH CTI6. |
| [16] | "Tup1-Ssn6 interacts with multiple class I histone deacetylases in vivo." Davie J.K., Edmondson D.G., Coco C.B., Dent S.Y. J. Biol. Chem. 278:50158-50162(2003) [PubMed: 14525981] [Abstract] Cited for: INTERACTION WITH HOS1; HOS2 AND RPD3. |
| [17] | "Histone-dependent association of Tup1-Ssn6 with repressed genes in vivo." Davie J.K., Trumbly R.J., Dent S.Y. Mol. Cell. Biol. 22:693-703(2002) [PubMed: 11784848] [Abstract] Cited for: FUNCTION OF THE CYC8-TUP1 COREPRESSOR COMPLEX. |
| [18] | "Recruitment of Tup1-Ssn6 by yeast hypoxic genes and chromatin-independent exclusion of TATA binding protein." Mennella T.A., Klinkenberg L.G., Zitomer R.S. Eukaryot. Cell 2:1288-1303(2003) [PubMed: 14665463] [Abstract] Cited for: FUNCTION OF THE CYC8-TUP1 COMPLEX. |
| [19] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [20] | "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-805, MASS SPECTROMETRY. |
| [21] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-815; SER-817 AND SER-943, MASS SPECTROMETRY. |
| [22] | "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-710, MASS SPECTROMETRY. |
| [23] | "Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-741; SER-780 AND SER-815, MASS SPECTROMETRY. |
| [24] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-429; THR-475; SER-710; SER-741; SER-755; SER-768; THR-779; SER-780; SER-793; SER-805; SER-815; SER-817; SER-866; SER-939 AND SER-943, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| M23440 Genomic DNA. Translation: AAA34545.1. M17826 Genomic DNA. Translation: AAA35103.1. X66247 Genomic DNA. Translation: CAA46973.1. X78993 Genomic DNA. Translation: CAA55615.1. Z35981 Genomic DNA. Translation: CAA85069.1. | |
| PIR | S25365. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:696N. |
| IntAct | P14922. 11 interactions. |
Proteomic databases | |
| PeptideAtlas | P14922. |
Genome annotation databases | |
| Ensembl | YBR112C. Saccharomyces cerevisiae. [Contig view] |
| GenomeReviews | Gene locus YBR112C in contig Y13134_GR. |
| KEGG | sce:YBR112C. |
Organism-specific databases | |
| CYGD | YBR112c. |
| SGD | S000000316. CYC8. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P14922. |
Gene expression databases | |
| ArrayExpress | P14922. |
| GermOnline | YBR112C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR001440. TPR-1. IPR011990. TPR-like_helical. IPR013026. TPR_region. IPR019734. TPR_repeat. [Graphical view] |
| Gene3D | G3DSA:1.25.40.10. TPR-like_helical. 2 hits. |
| Pfam | PF00515. TPR_1. 10 hits. [Graphical view] |
| SMART | SM00028. TPR. 10 hits. [Graphical view] |
| PROSITE | PS50005. TPR. 9 hits. PS50293. TPR_REGION. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | CYC8_YEAST | ||||||||
| Accession | Primary (citable) accession number: P14922 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome II Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names |

Clusters with


