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Protein

General transcriptional corepressor CYC8

Gene

CYC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the CYC8-TUP1 corepressor complex which is involved in the repression of many genes in a wide variety of physiological processes including heme-regulated and catabolite repressed genes. May also be involved in the derepression of at least some target genes. The complex is recruited to target genes by interaction with DNA-bound transcriptional repressors, like MATALPHA2, MIG1, RFX1 and SKO1. The complex recruits histone deacetylases to produce a repressive chromatin structure, interacts with hypoacetylated N-terminal tails of histones H3 and H4 that have been programmed for repression by the action of histone deacetylases and interferes directly with the transcriptional machinery by associating with the RNA polymerase II mediator complex.5 Publications

GO - Molecular functioni

  • histone deacetylase binding Source: SGD
  • transcriptional activator activity, RNA polymerase II transcription factor binding Source: SGD
  • transcriptional repressor activity, RNA polymerase II transcription factor binding Source: SGD

GO - Biological processi

  • chromatin remodeling Source: SGD
  • negative regulation of dipeptide transport by negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter during mitosis Source: SGD
  • nucleosome positioning Source: SGD
  • regulation of fatty acid biosynthetic process by regulation of transcription from RNA polymerase II promoter Source: SGD
  • regulation of response to DNA damage stimulus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Prion, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29073-MONOMER.
ReactomeiR-SCE-2559580. Oxidative Stress Induced Senescence.
R-SCE-3214842. HDMs demethylate histones.

Names & Taxonomyi

Protein namesi
Recommended name:
General transcriptional corepressor CYC8
Alternative name(s):
Glucose repression mediator protein CYC8
Gene namesi
Name:CYC8
Synonyms:CRT8, SSN6
Ordered Locus Names:YBR112C
ORF Names:YBR0908
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR112C.
SGDiS000000316. CYC8.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
  • transcriptional repressor complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Amyloid, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001063271 – 966General transcriptional corepressor CYC8Add BLAST966

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei429PhosphoserineCombined sources1
Modified residuei475PhosphothreonineCombined sources1
Modified residuei710PhosphoserineCombined sources1
Modified residuei741PhosphoserineCombined sources1
Modified residuei768PhosphoserineCombined sources1
Modified residuei815PhosphoserineCombined sources1
Modified residuei817PhosphoserineCombined sources1
Modified residuei866PhosphoserineCombined sources1
Modified residuei943PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP14922.
PRIDEiP14922.

PTM databases

iPTMnetiP14922.

Interactioni

Subunit structurei

Associates with TUP1 to form the CYC8-TUP1 (or TUP1-SSN6) corepressor complex that is composed of 4 copies of TUP1 and one copy of CYC8. Interacts with MATALPHA2, CTI6, MIG1, TUP1, SUT1, RFX1, PGD1, HOS1, HOS2 AND RPD3.10 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HOS2P530964EBI-18215,EBI-8475
PGD1P403563EBI-18215,EBI-13268
RFX1P487432EBI-18215,EBI-15036
RPD3P325616EBI-18215,EBI-15864
TUP1P166495EBI-18215,EBI-19654

GO - Molecular functioni

  • histone deacetylase binding Source: SGD

Protein-protein interaction databases

BioGridi32816. 122 interactors.
DIPiDIP-696N.
IntActiP14922. 10 interactors.
MINTiMINT-579675.

Structurei

3D structure databases

ProteinModelPortaliP14922.
SMRiP14922.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati46 – 79TPR 1Add BLAST34
Repeati80 – 113TPR 2Add BLAST34
Repeati114 – 147TPR 3Add BLAST34
Repeati150 – 183TPR 4Add BLAST34
Repeati187 – 220TPR 5Add BLAST34
Repeati224 – 257TPR 6Add BLAST34
Repeati262 – 295TPR 7Add BLAST34
Repeati296 – 329TPR 8Add BLAST34
Repeati330 – 363TPR 9Add BLAST34
Repeati364 – 398TPR 10Add BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni467 – 682Prion domain (PrD)Add BLAST216

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi15 – 30Poly-GlnAdd BLAST16

Domaini

The prion domain (PrD) is a Gln/Asn (Q/N)-rich domain, which is unstructured in its native, soluble form, and which forms a parallel in-register beta-sheet in its amyloid form.By similarity

Sequence similaritiesi

Belongs to the CYC8/SSN6 family.Curated
Contains 10 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

GeneTreeiENSGT00410000025758.
InParanoidiP14922.
KOiK06665.
OMAiHHPPRHH.
OrthoDBiEOG092C1SG7.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 10 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50005. TPR. 9 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14922-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPGGEQTIM EQPAQQQQQQ QQQQQQQQQQ AAVPQQPLDP LTQSTAETWL
60 70 80 90 100
SIASLAETLG DGDRAAMAYD ATLQFNPSSA KALTSLAHLY RSRDMFQRAA
110 120 130 140 150
ELYERALLVN PELSDVWATL GHCYLMLDDL QRAYNAYQQA LYHLSNPNVP
160 170 180 190 200
KLWHGIGILY DRYGSLDYAE EAFAKVLELD PHFEKANEIY FRLGIIYKHQ
210 220 230 240 250
GKWSQALECF RYILPQPPAP LQEWDIWFQL GSVLESMGEW QGAKEAYEHV
260 270 280 290 300
LAQNQHHAKV LQQLGCLYGM SNVQFYDPQK ALDYLLKSLE ADPSDATTWY
310 320 330 340 350
HLGRVHMIRT DYTAAYDAFQ QAVNRDSRNP IFWCSIGVLY YQISQYRDAL
360 370 380 390 400
DAYTRAIRLN PYISEVWYDL GTLYETCNNQ LSDALDAYKQ AARLDVNNVH
410 420 430 440 450
IRERLEALTK QLENPGNINK SNGAPTNASP APPPVILQPT LQPNDQGNPL
460 470 480 490 500
NTRISAQSAN ATASMVQQQH PAQQTPINSS ATMYSNGASP QLQAQAQAQA
510 520 530 540 550
QAQAQAQAQA QAQAQAQAQA QAQAQAQAQA QAQAQAHAQA QAQAQAQAQA
560 570 580 590 600
QAQAQAQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQLQP LPRQQLQQKG
610 620 630 640 650
VSVQMLNPQQ GQPYITQPTV IQAHQLQPFS TQAMEHPQSS QLPPQQQQLQ
660 670 680 690 700
SVQHPQQLQG QPQAQAPQPL IQHNVEQNVL PQKRYMEGAI HTLVDAAVSS
710 720 730 740 750
STHTENNTKS PRQPTHAIPT QAPATGITNA EPQVKKQKLN SPNSNINKLV
760 770 780 790 800
NTATSIEENA KSEVSNQSPA VVESNTNNTS QEEKPVKANS IPSVIGAQEP
810 820 830 840 850
PQEASPAEEA TKAASVSPST KPLNTEPESS SVQPTVSSES STTKANDQST
860 870 880 890 900
AETIELSTAT VPAEASPVED EVRQHSKEEN GTTEASAPST EEAEPAASRD
910 920 930 940 950
AEKQQDETAA TTITVIKPTL ETMETVKEEA KMREEEQTSQ EKSPQENTLP
960
RENVVRQVEE DENYDD
Length:966
Mass (Da):107,202
Last modified:October 5, 2010 - v2
Checksum:iFC2D0D0C1B181207
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti547Q → K in AAA34545 (PubMed:2854095).Curated1
Sequence conflicti547Q → K in AAA35103 (PubMed:3316983).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23440 Genomic DNA. Translation: AAA34545.1.
M17826 Genomic DNA. Translation: AAA35103.1.
X66247 Genomic DNA. Translation: CAA46973.1.
X78993 Genomic DNA. Translation: CAA55615.1.
Z35981 Genomic DNA. Translation: CAA85069.1.
BK006936 Genomic DNA. Translation: DAA07232.1.
PIRiS25365.
RefSeqiNP_009670.3. NM_001178460.3.

Genome annotation databases

EnsemblFungiiYBR112C; YBR112C; YBR112C.
GeneIDi852410.
KEGGisce:YBR112C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23440 Genomic DNA. Translation: AAA34545.1.
M17826 Genomic DNA. Translation: AAA35103.1.
X66247 Genomic DNA. Translation: CAA46973.1.
X78993 Genomic DNA. Translation: CAA55615.1.
Z35981 Genomic DNA. Translation: CAA85069.1.
BK006936 Genomic DNA. Translation: DAA07232.1.
PIRiS25365.
RefSeqiNP_009670.3. NM_001178460.3.

3D structure databases

ProteinModelPortaliP14922.
SMRiP14922.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32816. 122 interactors.
DIPiDIP-696N.
IntActiP14922. 10 interactors.
MINTiMINT-579675.

PTM databases

iPTMnetiP14922.

Proteomic databases

MaxQBiP14922.
PRIDEiP14922.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR112C; YBR112C; YBR112C.
GeneIDi852410.
KEGGisce:YBR112C.

Organism-specific databases

EuPathDBiFungiDB:YBR112C.
SGDiS000000316. CYC8.

Phylogenomic databases

GeneTreeiENSGT00410000025758.
InParanoidiP14922.
KOiK06665.
OMAiHHPPRHH.
OrthoDBiEOG092C1SG7.

Enzyme and pathway databases

BioCyciYEAST:G3O-29073-MONOMER.
ReactomeiR-SCE-2559580. Oxidative Stress Induced Senescence.
R-SCE-3214842. HDMs demethylate histones.

Miscellaneous databases

PROiP14922.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 10 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
PROSITEiPS50005. TPR. 9 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYC8_YEAST
AccessioniPrimary (citable) accession number: P14922
Secondary accession number(s): D6VQB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 5, 2010
Last modified: November 2, 2016
This is version 184 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

[OCT+] is the prion form of CYC8. [OCT+] is the result of a conformational change of the cellular CYC8 protein that becomes self-propagating and infectious. [OCT+]-aggregates sequester soluble CYC8, resulting in increased levels of iso-2-cytochrome c, defects in sporulation and mating, higher levels of invertase derepression and increased flocculation, reminiscent of a partial loss of function of the CYC8-TUP1 corepressor complex. [OCT+] can be cured by GdnHCl and by inactivation of the molecular chaperone HSP104, which is required for [OCT+] propagation. It is speculated that prion properties of transcription factors may generate an optimized phenotypic heterogeneity that buffers yeast populations against diverse environmental insults.1 Publication
Present with 3890 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.