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P14916

- URE23_HELPY

UniProt

P14916 - URE23_HELPY

Protein

Urease subunit alpha

Gene

ureA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (01 May 1992)
      Previous versions | rss
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    Functioni

    Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach.1 Publication

    Catalytic activityi

    Urea + H2O = CO2 + 2 NH3.2 PublicationsUniRule annotation

    Kineticsi

    1. KM=0.48 mM for urea1 Publication

    Vmax=1.1 mmol/min/mg enzyme1 Publication

    pH dependencei

    Optimum pH is 8.0. Active from pH 4.0 to 10.0. In unbuffered solutions, the dodecameric complex is active at pH 3.0.2 Publications

    Pathwayi

    GO - Molecular functioni

    1. nickel cation binding Source: InterPro
    2. urease activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. pathogenesis Source: UniProtKB-KW
    2. urea catabolic process Source: CACAO

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Virulence

    Enzyme and pathway databases

    BioCyciHPY:HP0073-MONOMER.
    UniPathwayiUPA00258; UER00370.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
    Alternative name(s):
    Urea amidohydrolase subunit alphaUniRule annotation
    Gene namesi
    Name:ureAUniRule annotation
    Synonyms:hpuA
    Ordered Locus Names:HP_0073
    OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
    Taxonomic identifieri85962 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000000429: Chromosome

    Subcellular locationi

    Cytoplasm 1 PublicationUniRule annotation
    Note: Also associates with the outer membrane upon autolysis of neighboring bacteria.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Cells do not express urease.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 238238Urease subunit alphaPRO_0000098075Add
    BLAST

    Proteomic databases

    PRIDEiP14916.

    Expressioni

    Inductioni

    By nickel ions.1 Publication

    Interactioni

    Subunit structurei

    Heterohexamer of 3 UreA (alpha) and 3 UreB (beta) subunits. Four heterohexamers assemble to form a 16 nm dodecameric complex.1 PublicationUniRule annotation

    Protein-protein interaction databases

    DIPiDIP-3146N.
    IntActiP14916. 6 interactions.
    MINTiMINT-180633.
    STRINGi85962.HP0073.

    Structurei

    Secondary structure

    1
    238
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 2521
    Helixi32 – 4918
    Helixi54 – 607
    Helixi61 – 633
    Turni67 – 693
    Helixi74 – 774
    Beta strandi80 – 878
    Beta strandi90 – 978
    Beta strandi117 – 1193
    Turni120 – 1234
    Beta strandi128 – 1336
    Beta strandi135 – 1373
    Beta strandi139 – 1424
    Helixi147 – 1493
    Beta strandi154 – 1563
    Helixi158 – 1614
    Beta strandi164 – 1663
    Beta strandi173 – 1764
    Beta strandi181 – 1888
    Helixi208 – 22114

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1E9YX-ray3.00A1-238[»]
    1E9ZX-ray3.00A1-238[»]
    ProteinModelPortaliP14916.
    SMRiP14916. Positions 1-238.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP14916.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 102102Urease gammaAdd
    BLAST
    Regioni103 – 238136Urease betaAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the urease gamma subunit family.UniRule annotation
    In the C-terminal section; belongs to the urease beta subunit family.UniRule annotation

    Phylogenomic databases

    KOiK14048.
    OMAiFAHEEER.
    OrthoDBiEOG69PQ9G.

    Family and domain databases

    Gene3Di2.10.150.10. 1 hit.
    3.30.280.10. 1 hit.
    HAMAPiMF_01954. Urease_beta.
    MF_01955. Urease_beta_gamma.
    InterProiIPR002019. Urease_beta.
    IPR008223. Urease_gamma-beta_su.
    IPR002026. Urease_gamma/gamma-beta_su.
    [Graphical view]
    PfamiPF00699. Urease_beta. 1 hit.
    PF00547. Urease_gamma. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001225. Urease_gammabeta. 1 hit.
    ProDomiPD002319. Urease_gamma_reg. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF51278. SSF51278. 1 hit.
    SSF54111. SSF54111. 1 hit.
    TIGRFAMsiTIGR00192. urease_beta. 1 hit.
    TIGR00193. urease_gam. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P14916-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLTPKELDK LMLHYAGELA KKRKEKGIKL NYVEAVALIS AHIMEEARAG    50
    KKTAAELMQE GRTLLKPDDV MDGVASMIHE VGIEAMFPDG TKLVTVHTPI 100
    EANGKLVPGE LFLKNEDITI NEGKKAVSVK VKNVGDRPVQ IGSHFHFFEV 150
    NRCLDFDREK TFGKRLDIAS GTAVRFEPGE EKSVELIDIG GNRRIFGFNA 200
    LVDRQADNES KKIALHRAKE RGFHGAKSDD NYVKTIKE 238
    Length:238
    Mass (Da):26,540
    Last modified:May 1, 1992 - v2
    Checksum:i4E77328669CD9A2D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti14 – 141H → S AA sequence (PubMed:2318539)Curated
    Sequence conflicti37 – 371A → R in CAA34932. (PubMed:2326167)Curated
    Sequence conflicti49 – 491A → R in CAA34932. (PubMed:2326167)Curated
    Sequence conflicti132 – 1332KN → PP in CAA34932. (PubMed:2326167)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17079 Genomic DNA. Translation: CAA34932.1.
    M60398 Genomic DNA. Translation: AAA25020.1.
    AB032429 Genomic DNA. Translation: BAA84532.1.
    AE000511 Genomic DNA. Translation: AAD07144.1.
    PIRiA38537. URKCAP.
    RefSeqiNP_206873.1. NC_000915.1.
    WP_000779223.1. NC_018939.1.
    YP_006933995.1. NC_018939.1.

    Genome annotation databases

    EnsemblBacteriaiAAD07144; AAD07144; HP_0073.
    GeneIDi13869249.
    900171.
    KEGGiheo:C694_00355.
    hpy:HP0073.
    PATRICi20591349. VBIHelPyl33062_0076.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Going unnoticed - Issue 95 of June 2008

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17079 Genomic DNA. Translation: CAA34932.1 .
    M60398 Genomic DNA. Translation: AAA25020.1 .
    AB032429 Genomic DNA. Translation: BAA84532.1 .
    AE000511 Genomic DNA. Translation: AAD07144.1 .
    PIRi A38537. URKCAP.
    RefSeqi NP_206873.1. NC_000915.1.
    WP_000779223.1. NC_018939.1.
    YP_006933995.1. NC_018939.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1E9Y X-ray 3.00 A 1-238 [» ]
    1E9Z X-ray 3.00 A 1-238 [» ]
    ProteinModelPortali P14916.
    SMRi P14916. Positions 1-238.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-3146N.
    IntActi P14916. 6 interactions.
    MINTi MINT-180633.
    STRINGi 85962.HP0073.

    Proteomic databases

    PRIDEi P14916.

    Protocols and materials databases

    DNASUi 900171.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD07144 ; AAD07144 ; HP_0073 .
    GeneIDi 13869249.
    900171.
    KEGGi heo:C694_00355.
    hpy:HP0073.
    PATRICi 20591349. VBIHelPyl33062_0076.

    Phylogenomic databases

    KOi K14048.
    OMAi FAHEEER.
    OrthoDBi EOG69PQ9G.

    Enzyme and pathway databases

    UniPathwayi UPA00258 ; UER00370 .
    BioCyci HPY:HP0073-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P14916.

    Family and domain databases

    Gene3Di 2.10.150.10. 1 hit.
    3.30.280.10. 1 hit.
    HAMAPi MF_01954. Urease_beta.
    MF_01955. Urease_beta_gamma.
    InterProi IPR002019. Urease_beta.
    IPR008223. Urease_gamma-beta_su.
    IPR002026. Urease_gamma/gamma-beta_su.
    [Graphical view ]
    Pfami PF00699. Urease_beta. 1 hit.
    PF00547. Urease_gamma. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001225. Urease_gammabeta. 1 hit.
    ProDomi PD002319. Urease_gamma_reg. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF51278. SSF51278. 1 hit.
    SSF54111. SSF54111. 1 hit.
    TIGRFAMsi TIGR00192. urease_beta. 1 hit.
    TIGR00193. urease_gam. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of two genes from Helicobacter pylori encoding for urease subunits."
      Clayton C.L., Pallen M.J., Kleanthous H., Wren B.W., Tabaqchali S.
      Nucleic Acids Res. 18:362-362(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: CPM630.
    2. "Shuttle cloning and nucleotide sequences of Helicobacter pylori genes responsible for urease activity."
      Labigne A., Cussac V., Courcoux P.
      J. Bacteriol. 173:1920-1931(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 85P.
    3. "Identification of the urease operon in Helicobacter pylori and its control by mRNA decay in response to pH."
      Akada J.K., Shirai M., Takeuchi H., Tsuda M., Nakazawa T.
      Mol. Microbiol. 36:1071-1084(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HPK5.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700392 / 26695.
    5. "Purification and N-terminal analysis of urease from Helicobacter pylori."
      Hu L.-T., Mobley H.L.T.
      Infect. Immun. 58:992-998(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-20.
    6. "Purification and characterization of urease from Helicobacter pylori."
      Dunn B.E., Campbell G.P., Perez-Perez G.I., Blaser M.J.
      J. Biol. Chem. 265:9464-9469(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-20, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH UREB.
    7. "Purification and characterization of the urease enzymes of Helicobacter species from humans and animals."
      Turbett G.R., Hoej P.B., Horne R., Mee B.J.
      Infect. Immun. 60:5259-5266(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-20.
      Strain: ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487.
    8. "Expression of Helicobacter pylori urease genes in Escherichia coli grown under nitrogen-limiting conditions."
      Cussac V., Ferrero R.L., Labigne A.
      J. Bacteriol. 174:2466-2473(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
      Strain: 85P.
    9. "Purification of recombinant Helicobacter pylori urease apoenzyme encoded by ureA and ureB."
      Hu L.-T., Foxall P.A., Russell R., Mobley H.L.T.
      Infect. Immun. 60:2657-2666(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY.
    10. "A urease-negative mutant of Helicobacter pylori constructed by allelic exchange mutagenesis lacks the ability to colonize the nude mouse stomach."
      Tsuda M., Karita M., Morshed M.G., Okita K., Nakazawa T.
      Infect. Immun. 62:3586-3589(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Surface localization of Helicobacter pylori urease and a heat shock protein homolog requires bacterial autolysis."
      Phadnis S.H., Parlow M.H., Levy M., Ilver D., Caulkins C.M., Connors J.B., Dunn B.E.
      Infect. Immun. 64:905-912(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    12. "Nickel-responsive induction of urease expression in Helicobacter pylori is mediated at the transcriptional level."
      van Vliet A.H.M., Kuipers E.J., Waidner B., Davies B.J., de Vries N., Penn C.W., Vandenbroucke-Grauls C.M.J.E., Kist M., Bereswill S., Kusters J.G.
      Infect. Immun. 69:4891-4897(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    13. "Bacterial factors that mediate colonization of the stomach and virulence of Helicobacter pylori."
      Clyne M., Dolan B., Reeves E.P.
      FEMS Microbiol. Lett. 268:135-143(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON VIRULENCE OF H.PYLORI.
    14. "Supramolecular assembly and acid resistance of Helicobacter pylori urease."
      Ha N.-C., Oh S.-T., Sung J.Y., Cha K.A., Lee M.H., Oh B.-H.
      Nat. Struct. Biol. 8:505-509(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS), SUBUNIT STRUCTURE, PH DEPENDENCE.

    Entry informationi

    Entry nameiURE23_HELPY
    AccessioniPrimary (citable) accession number: P14916
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The novel dodecameric structure of the enzyme may allow it to remain active at the cell surface at acidic gastric pH. Within this dodecameric structure the 12 active sites are clustered within the interior of the proteinaceous shell. This may allow a high local concentration of ammonia within the enzyme which may protect the nickel-chelating groups from protonation.

    Caution

    The orthologous protein is known as the gamma/beta subunit (UreAB) in most other bacteria.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Helicobacter pylori
      Helicobacter pylori (strain 26695): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3