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Protein

Urease subunit alpha

Gene

ureA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach.1 Publication

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation2 Publications

Kineticsi

  1. KM=0.48 mM for urea1 Publication
  1. Vmax=1.1 mmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 8.0. Active from pH 4.0 to 10.0. In unbuffered solutions, the dodecameric complex is active at pH 3.0.2 Publications

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease (ureB), Urease subunit beta (ureB), Urease subunit alpha (ureA), Urease (ureA)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

GO - Molecular functioni

GO - Biological processi

  • pathogenesis Source: UniProtKB-KW
  • urea catabolic process Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciHPY:HP0073-MONOMER.
UniPathwayiUPA00258; UER00370.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureAUniRule annotation
Synonyms:hpuA
Ordered Locus Names:HP_0073
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

  • Note: Also associates with the outer membrane upon autolysis of neighboring bacteria.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells do not express urease.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000980751 – 238Urease subunit alphaAdd BLAST238

Proteomic databases

PaxDbiP14916.

Expressioni

Inductioni

By nickel ions.1 Publication

Interactioni

Subunit structurei

Heterohexamer of 3 UreA (alpha) and 3 UreB (beta) subunits. Four heterohexamers assemble to form a 16 nm dodecameric complex.UniRule annotation1 Publication

Protein-protein interaction databases

DIPiDIP-3146N.
IntActiP14916. 6 interactors.
MINTiMINT-180633.
STRINGi85962.HP0073.

Structurei

Secondary structure

1238
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 25Combined sources21
Helixi32 – 49Combined sources18
Helixi54 – 60Combined sources7
Helixi61 – 63Combined sources3
Turni67 – 69Combined sources3
Helixi74 – 77Combined sources4
Beta strandi80 – 87Combined sources8
Beta strandi90 – 97Combined sources8
Beta strandi117 – 119Combined sources3
Turni120 – 123Combined sources4
Beta strandi128 – 133Combined sources6
Beta strandi135 – 137Combined sources3
Beta strandi139 – 142Combined sources4
Helixi147 – 149Combined sources3
Beta strandi154 – 156Combined sources3
Helixi158 – 161Combined sources4
Beta strandi164 – 166Combined sources3
Beta strandi173 – 176Combined sources4
Beta strandi181 – 188Combined sources8
Helixi208 – 221Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E9YX-ray3.00A1-238[»]
1E9ZX-ray3.00A1-238[»]
ProteinModelPortaliP14916.
SMRiP14916.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14916.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 102Urease gammaAdd BLAST102
Regioni103 – 238Urease betaAdd BLAST136

Sequence similaritiesi

In the N-terminal section; belongs to the urease gamma subunit family.UniRule annotation
In the C-terminal section; belongs to the urease beta subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108YZ9. Bacteria.
COG0831. LUCA.
COG0832. LUCA.
KOiK14048.
OMAiISMEIME.

Family and domain databases

CDDicd00407. Urease_beta. 1 hit.
cd00390. Urease_gamma. 1 hit.
Gene3Di2.10.150.10. 1 hit.
3.30.280.10. 1 hit.
HAMAPiMF_01954. Urease_beta. 1 hit.
MF_01955. Urease_beta_gamma. 1 hit.
InterProiIPR002019. Urease_beta.
IPR008223. Urease_gamma-beta_su.
IPR002026. Urease_gamma/gamma-beta_su.
[Graphical view]
PfamiPF00699. Urease_beta. 1 hit.
PF00547. Urease_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF001225. Urease_gammabeta. 1 hit.
ProDomiPD002319. Urease_gamma_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51278. SSF51278. 1 hit.
SSF54111. SSF54111. 1 hit.
TIGRFAMsiTIGR00192. urease_beta. 1 hit.
TIGR00193. urease_gam. 1 hit.

Sequencei

Sequence statusi: Complete.

P14916-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLTPKELDK LMLHYAGELA KKRKEKGIKL NYVEAVALIS AHIMEEARAG
60 70 80 90 100
KKTAAELMQE GRTLLKPDDV MDGVASMIHE VGIEAMFPDG TKLVTVHTPI
110 120 130 140 150
EANGKLVPGE LFLKNEDITI NEGKKAVSVK VKNVGDRPVQ IGSHFHFFEV
160 170 180 190 200
NRCLDFDREK TFGKRLDIAS GTAVRFEPGE EKSVELIDIG GNRRIFGFNA
210 220 230
LVDRQADNES KKIALHRAKE RGFHGAKSDD NYVKTIKE
Length:238
Mass (Da):26,540
Last modified:May 1, 1992 - v2
Checksum:i4E77328669CD9A2D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14H → S AA sequence (PubMed:2318539).Curated1
Sequence conflicti37A → R in CAA34932 (PubMed:2326167).Curated1
Sequence conflicti49A → R in CAA34932 (PubMed:2326167).Curated1
Sequence conflicti132 – 133KN → PP in CAA34932 (PubMed:2326167).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17079 Genomic DNA. Translation: CAA34932.1.
M60398 Genomic DNA. Translation: AAA25020.1.
AB032429 Genomic DNA. Translation: BAA84532.1.
AE000511 Genomic DNA. Translation: AAD07144.1.
PIRiA38537. URKCAP.
RefSeqiNP_206873.1. NC_000915.1.
WP_000779223.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07144; AAD07144; HP_0073.
GeneIDi900171.
KEGGiheo:C694_00355.
hpy:HP0073.
PATRICi20591349. VBIHelPyl33062_0076.

Cross-referencesi

Web resourcesi

Protein Spotlight

Going unnoticed - Issue 95 of June 2008

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17079 Genomic DNA. Translation: CAA34932.1.
M60398 Genomic DNA. Translation: AAA25020.1.
AB032429 Genomic DNA. Translation: BAA84532.1.
AE000511 Genomic DNA. Translation: AAD07144.1.
PIRiA38537. URKCAP.
RefSeqiNP_206873.1. NC_000915.1.
WP_000779223.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E9YX-ray3.00A1-238[»]
1E9ZX-ray3.00A1-238[»]
ProteinModelPortaliP14916.
SMRiP14916.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3146N.
IntActiP14916. 6 interactors.
MINTiMINT-180633.
STRINGi85962.HP0073.

Proteomic databases

PaxDbiP14916.

Protocols and materials databases

DNASUi900171.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07144; AAD07144; HP_0073.
GeneIDi900171.
KEGGiheo:C694_00355.
hpy:HP0073.
PATRICi20591349. VBIHelPyl33062_0076.

Phylogenomic databases

eggNOGiENOG4108YZ9. Bacteria.
COG0831. LUCA.
COG0832. LUCA.
KOiK14048.
OMAiISMEIME.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.
BioCyciHPY:HP0073-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP14916.

Family and domain databases

CDDicd00407. Urease_beta. 1 hit.
cd00390. Urease_gamma. 1 hit.
Gene3Di2.10.150.10. 1 hit.
3.30.280.10. 1 hit.
HAMAPiMF_01954. Urease_beta. 1 hit.
MF_01955. Urease_beta_gamma. 1 hit.
InterProiIPR002019. Urease_beta.
IPR008223. Urease_gamma-beta_su.
IPR002026. Urease_gamma/gamma-beta_su.
[Graphical view]
PfamiPF00699. Urease_beta. 1 hit.
PF00547. Urease_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF001225. Urease_gammabeta. 1 hit.
ProDomiPD002319. Urease_gamma_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51278. SSF51278. 1 hit.
SSF54111. SSF54111. 1 hit.
TIGRFAMsiTIGR00192. urease_beta. 1 hit.
TIGR00193. urease_gam. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiURE23_HELPY
AccessioniPrimary (citable) accession number: P14916
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: May 1, 1992
Last modified: November 2, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The novel dodecameric structure of the enzyme may allow it to remain active at the cell surface at acidic gastric pH. Within this dodecameric structure the 12 active sites are clustered within the interior of the proteinaceous shell. This may allow a high local concentration of ammonia within the enzyme which may protect the nickel-chelating groups from protonation.

Caution

The orthologous protein is known as the gamma/beta subunit (UreAB) in most other bacteria.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.