Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Urease subunit alpha

Gene

ureA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach.1 Publication

Miscellaneous

The novel dodecameric structure of the enzyme may allow it to remain active at the cell surface at acidic gastric pH. Within this dodecameric structure the 12 active sites are clustered within the interior of the proteinaceous shell. This may allow a high local concentration of ammonia within the enzyme which may protect the nickel-chelating groups from protonation.

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.2 Publications

Kineticsi

  1. KM=0.48 mM for urea1 Publication
  1. Vmax=1.1 mmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 8.0. Active from pH 4.0 to 10.0. In unbuffered solutions, the dodecameric complex is active at pH 3.0.2 Publications

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).
Proteins known to be involved in this subpathway in this organism are:
  1. Urease (ureB), Urease subunit beta (ureB), Urease subunit alpha (ureA), Urease (ureA)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

GO - Molecular functioni

GO - Biological processi

  • pathogenesis Source: UniProtKB-KW
  • urea catabolic process Source: CACAO

Keywordsi

Molecular functionHydrolase
Biological processVirulence

Enzyme and pathway databases

BioCyciHPY:HP0073-MONOMER.
UniPathwayiUPA00258; UER00370.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alpha (EC:3.5.1.5)
Alternative name(s):
Urea amidohydrolase subunit alpha
Gene namesi
Name:ureA
Synonyms:hpuA
Ordered Locus Names:HP_0073
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm 1 Publication
  • Note: Also associates with the outer membrane upon autolysis of neighboring bacteria.

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells do not express urease.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000980751 – 238Urease subunit alphaAdd BLAST238

Proteomic databases

PaxDbiP14916.

Expressioni

Inductioni

By nickel ions.1 Publication

Interactioni

Subunit structurei

Heterohexamer of 3 UreA (alpha) and 3 UreB (beta) subunits. Four heterohexamers assemble to form a 16 nm dodecameric complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ureBP699962EBI-7737170,EBI-7566591

Protein-protein interaction databases

DIPiDIP-3146N.
IntActiP14916. 7 interactors.
MINTiMINT-180633.
STRINGi85962.HP0073.

Chemistry databases

BindingDBiP14916.

Structurei

Secondary structure

1238
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 25Combined sources21
Helixi32 – 49Combined sources18
Helixi54 – 60Combined sources7
Helixi61 – 63Combined sources3
Turni67 – 69Combined sources3
Helixi74 – 77Combined sources4
Beta strandi80 – 87Combined sources8
Beta strandi90 – 97Combined sources8
Beta strandi117 – 119Combined sources3
Turni120 – 123Combined sources4
Beta strandi128 – 133Combined sources6
Beta strandi135 – 137Combined sources3
Beta strandi139 – 142Combined sources4
Helixi147 – 149Combined sources3
Beta strandi154 – 156Combined sources3
Helixi158 – 161Combined sources4
Beta strandi164 – 166Combined sources3
Beta strandi173 – 176Combined sources4
Beta strandi181 – 188Combined sources8
Helixi208 – 221Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E9YX-ray3.00A1-238[»]
1E9ZX-ray3.00A1-238[»]
ProteinModelPortaliP14916.
SMRiP14916.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14916.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 102Urease gammaAdd BLAST102
Regioni103 – 238Urease betaAdd BLAST136

Sequence similaritiesi

In the N-terminal section; belongs to the urease gamma subunit family.
In the C-terminal section; belongs to the urease beta subunit family.

Phylogenomic databases

eggNOGiENOG4108YZ9. Bacteria.
COG0831. LUCA.
COG0832. LUCA.
KOiK14048.
OMAiTSHFHFF.

Family and domain databases

CDDicd00407. Urease_beta. 1 hit.
cd00390. Urease_gamma. 1 hit.
Gene3Di2.10.150.10. 1 hit.
3.30.280.10. 1 hit.
HAMAPiMF_01954. Urease_beta. 1 hit.
MF_01955. Urease_beta_gamma. 1 hit.
InterProiView protein in InterPro
IPR002019. Urease_beta.
IPR036461. Urease_betasu_sf.
IPR008223. Urease_gamma-beta_su.
IPR002026. Urease_gamma/gamma-beta_su.
IPR036463. Urease_gamma_sf.
PfamiView protein in Pfam
PF00699. Urease_beta. 1 hit.
PF00547. Urease_gamma. 1 hit.
PIRSFiPIRSF001225. Urease_gammabeta. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002319. Urease_gamma_reg. 1 hit.
SUPFAMiSSF51278. SSF51278. 1 hit.
SSF54111. SSF54111. 1 hit.
TIGRFAMsiTIGR00192. urease_beta. 1 hit.
TIGR00193. urease_gam. 1 hit.

Sequencei

Sequence statusi: Complete.

P14916-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLTPKELDK LMLHYAGELA KKRKEKGIKL NYVEAVALIS AHIMEEARAG
60 70 80 90 100
KKTAAELMQE GRTLLKPDDV MDGVASMIHE VGIEAMFPDG TKLVTVHTPI
110 120 130 140 150
EANGKLVPGE LFLKNEDITI NEGKKAVSVK VKNVGDRPVQ IGSHFHFFEV
160 170 180 190 200
NRCLDFDREK TFGKRLDIAS GTAVRFEPGE EKSVELIDIG GNRRIFGFNA
210 220 230
LVDRQADNES KKIALHRAKE RGFHGAKSDD NYVKTIKE
Length:238
Mass (Da):26,540
Last modified:May 1, 1992 - v2
Checksum:i4E77328669CD9A2D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14H → S AA sequence (PubMed:2318539).Curated1
Sequence conflicti37A → R in CAA34932 (PubMed:2326167).Curated1
Sequence conflicti49A → R in CAA34932 (PubMed:2326167).Curated1
Sequence conflicti132 – 133KN → PP in CAA34932 (PubMed:2326167).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17079 Genomic DNA. Translation: CAA34932.1.
M60398 Genomic DNA. Translation: AAA25020.1.
AB032429 Genomic DNA. Translation: BAA84532.1.
AE000511 Genomic DNA. Translation: AAD07144.1.
PIRiA38537. URKCAP.
RefSeqiNP_206873.1. NC_000915.1.
WP_000779223.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07144; AAD07144; HP_0073.
GeneIDi900171.
KEGGiheo:C694_00355.
hpy:HP0073.
PATRICifig|85962.47.peg.77.

Similar proteinsi

Entry informationi

Entry nameiURE23_HELPY
AccessioniPrimary (citable) accession number: P14916
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: May 1, 1992
Last modified: November 22, 2017
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

The orthologous protein is known as the gamma/beta subunit (UreAB) in most other bacteria.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  5. SIMILARITY comments
    Index of protein domains and families