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P14916

- URE23_HELPY

UniProt

P14916 - URE23_HELPY

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Protein
Urease subunit alpha
Gene
ureA, hpuA, HP_0073
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Ammonia produced by ureolysis increases the gastric pH thereby providing an environment permissive for colonization of the stomach.1 Publication

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.2 Publications

Kineticsi

  1. KM=0.48 mM for urea1 Publication

Vmax=1.1 mmol/min/mg enzyme

pH dependencei

Optimum pH is 8.0. Active from pH 4.0 to 10.0. In unbuffered solutions, the dodecameric complex is active at pH 3.0.

Pathwayi

GO - Molecular functioni

  1. nickel cation binding Source: InterPro
  2. urease activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. pathogenesis Source: UniProtKB-KW
  2. urea catabolic process Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BioCyciHPY:HP0073-MONOMER.
UniPathwayiUPA00258; UER00370.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alpha (EC:3.5.1.5)
Alternative name(s):
Urea amidohydrolase subunit alpha
Gene namesi
Name:ureA
Synonyms:hpuA
Ordered Locus Names:HP_0073
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000000429: Chromosome

Subcellular locationi

Cytoplasm
Note: Also associates with the outer membrane upon autolysis of neighboring bacteria.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells do not express urease.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 238238Urease subunit alphaUniRule annotation
PRO_0000098075Add
BLAST

Proteomic databases

PRIDEiP14916.

Expressioni

Inductioni

By nickel ions.1 Publication

Interactioni

Subunit structurei

Heterohexamer of 3 UreA (alpha) and 3 UreB (beta) subunits. Four heterohexamers assemble to form a 16 nm dodecameric complex.1 Publication

Protein-protein interaction databases

DIPiDIP-3146N.
IntActiP14916. 6 interactions.
MINTiMINT-180633.
STRINGi85962.HP0073.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 2521
Helixi32 – 4918
Helixi54 – 607
Helixi61 – 633
Turni67 – 693
Helixi74 – 774
Beta strandi80 – 878
Beta strandi90 – 978
Beta strandi117 – 1193
Turni120 – 1234
Beta strandi128 – 1336
Beta strandi135 – 1373
Beta strandi139 – 1424
Helixi147 – 1493
Beta strandi154 – 1563
Helixi158 – 1614
Beta strandi164 – 1663
Beta strandi173 – 1764
Beta strandi181 – 1888
Helixi208 – 22114

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E9YX-ray3.00A1-238[»]
1E9ZX-ray3.00A1-238[»]
ProteinModelPortaliP14916.
SMRiP14916. Positions 1-238.

Miscellaneous databases

EvolutionaryTraceiP14916.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 102102Urease gammaUniRule annotation
Add
BLAST
Regioni103 – 238136Urease betaUniRule annotation
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the urease gamma subunit family.
In the C-terminal section; belongs to the urease beta subunit family.

Phylogenomic databases

KOiK14048.
OMAiFAHEEER.
OrthoDBiEOG69PQ9G.

Family and domain databases

Gene3Di2.10.150.10. 1 hit.
3.30.280.10. 1 hit.
HAMAPiMF_01954. Urease_beta.
MF_01955. Urease_beta_gamma.
InterProiIPR002019. Urease_beta.
IPR008223. Urease_gamma-beta_su.
IPR002026. Urease_gamma/gamma-beta_su.
[Graphical view]
PfamiPF00699. Urease_beta. 1 hit.
PF00547. Urease_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF001225. Urease_gammabeta. 1 hit.
ProDomiPD002319. Urease_gamma_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51278. SSF51278. 1 hit.
SSF54111. SSF54111. 1 hit.
TIGRFAMsiTIGR00192. urease_beta. 1 hit.
TIGR00193. urease_gam. 1 hit.

Sequencei

Sequence statusi: Complete.

P14916-1 [UniParc]FASTAAdd to Basket

« Hide

MKLTPKELDK LMLHYAGELA KKRKEKGIKL NYVEAVALIS AHIMEEARAG    50
KKTAAELMQE GRTLLKPDDV MDGVASMIHE VGIEAMFPDG TKLVTVHTPI 100
EANGKLVPGE LFLKNEDITI NEGKKAVSVK VKNVGDRPVQ IGSHFHFFEV 150
NRCLDFDREK TFGKRLDIAS GTAVRFEPGE EKSVELIDIG GNRRIFGFNA 200
LVDRQADNES KKIALHRAKE RGFHGAKSDD NYVKTIKE 238
Length:238
Mass (Da):26,540
Last modified:May 1, 1992 - v2
Checksum:i4E77328669CD9A2D
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141H → S AA sequence 1 Publication
Sequence conflicti37 – 371A → R in CAA34932. 1 Publication
Sequence conflicti49 – 491A → R in CAA34932. 1 Publication
Sequence conflicti132 – 1332KN → PP in CAA34932. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17079 Genomic DNA. Translation: CAA34932.1.
M60398 Genomic DNA. Translation: AAA25020.1.
AB032429 Genomic DNA. Translation: BAA84532.1.
AE000511 Genomic DNA. Translation: AAD07144.1.
PIRiA38537. URKCAP.
RefSeqiNP_206873.1. NC_000915.1.
WP_000779223.1. NC_018939.1.
YP_006933995.1. NC_018939.1.

Genome annotation databases

EnsemblBacteriaiAAD07144; AAD07144; HP_0073.
GeneIDi13869249.
900171.
KEGGiheo:C694_00355.
hpy:HP0073.
PATRICi20591349. VBIHelPyl33062_0076.

Cross-referencesi

Web resourcesi

Protein Spotlight

Going unnoticed - Issue 95 of June 2008

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17079 Genomic DNA. Translation: CAA34932.1 .
M60398 Genomic DNA. Translation: AAA25020.1 .
AB032429 Genomic DNA. Translation: BAA84532.1 .
AE000511 Genomic DNA. Translation: AAD07144.1 .
PIRi A38537. URKCAP.
RefSeqi NP_206873.1. NC_000915.1.
WP_000779223.1. NC_018939.1.
YP_006933995.1. NC_018939.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1E9Y X-ray 3.00 A 1-238 [» ]
1E9Z X-ray 3.00 A 1-238 [» ]
ProteinModelPortali P14916.
SMRi P14916. Positions 1-238.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-3146N.
IntActi P14916. 6 interactions.
MINTi MINT-180633.
STRINGi 85962.HP0073.

Proteomic databases

PRIDEi P14916.

Protocols and materials databases

DNASUi 900171.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD07144 ; AAD07144 ; HP_0073 .
GeneIDi 13869249.
900171.
KEGGi heo:C694_00355.
hpy:HP0073.
PATRICi 20591349. VBIHelPyl33062_0076.

Phylogenomic databases

KOi K14048.
OMAi FAHEEER.
OrthoDBi EOG69PQ9G.

Enzyme and pathway databases

UniPathwayi UPA00258 ; UER00370 .
BioCyci HPY:HP0073-MONOMER.

Miscellaneous databases

EvolutionaryTracei P14916.

Family and domain databases

Gene3Di 2.10.150.10. 1 hit.
3.30.280.10. 1 hit.
HAMAPi MF_01954. Urease_beta.
MF_01955. Urease_beta_gamma.
InterProi IPR002019. Urease_beta.
IPR008223. Urease_gamma-beta_su.
IPR002026. Urease_gamma/gamma-beta_su.
[Graphical view ]
Pfami PF00699. Urease_beta. 1 hit.
PF00547. Urease_gamma. 1 hit.
[Graphical view ]
PIRSFi PIRSF001225. Urease_gammabeta. 1 hit.
ProDomi PD002319. Urease_gamma_reg. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF51278. SSF51278. 1 hit.
SSF54111. SSF54111. 1 hit.
TIGRFAMsi TIGR00192. urease_beta. 1 hit.
TIGR00193. urease_gam. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of two genes from Helicobacter pylori encoding for urease subunits."
    Clayton C.L., Pallen M.J., Kleanthous H., Wren B.W., Tabaqchali S.
    Nucleic Acids Res. 18:362-362(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CPM630.
  2. "Shuttle cloning and nucleotide sequences of Helicobacter pylori genes responsible for urease activity."
    Labigne A., Cussac V., Courcoux P.
    J. Bacteriol. 173:1920-1931(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 85P.
  3. "Identification of the urease operon in Helicobacter pylori and its control by mRNA decay in response to pH."
    Akada J.K., Shirai M., Takeuchi H., Tsuda M., Nakazawa T.
    Mol. Microbiol. 36:1071-1084(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HPK5.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700392 / 26695.
  5. "Purification and N-terminal analysis of urease from Helicobacter pylori."
    Hu L.-T., Mobley H.L.T.
    Infect. Immun. 58:992-998(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-20.
  6. "Purification and characterization of urease from Helicobacter pylori."
    Dunn B.E., Campbell G.P., Perez-Perez G.I., Blaser M.J.
    J. Biol. Chem. 265:9464-9469(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-20, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, INTERACTION WITH UREB.
  7. "Purification and characterization of the urease enzymes of Helicobacter species from humans and animals."
    Turbett G.R., Hoej P.B., Horne R., Mee B.J.
    Infect. Immun. 60:5259-5266(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-20.
    Strain: ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487.
  8. "Expression of Helicobacter pylori urease genes in Escherichia coli grown under nitrogen-limiting conditions."
    Cussac V., Ferrero R.L., Labigne A.
    J. Bacteriol. 174:2466-2473(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: 85P.
  9. "Purification of recombinant Helicobacter pylori urease apoenzyme encoded by ureA and ureB."
    Hu L.-T., Foxall P.A., Russell R., Mobley H.L.T.
    Infect. Immun. 60:2657-2666(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.
  10. "A urease-negative mutant of Helicobacter pylori constructed by allelic exchange mutagenesis lacks the ability to colonize the nude mouse stomach."
    Tsuda M., Karita M., Morshed M.G., Okita K., Nakazawa T.
    Infect. Immun. 62:3586-3589(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Surface localization of Helicobacter pylori urease and a heat shock protein homolog requires bacterial autolysis."
    Phadnis S.H., Parlow M.H., Levy M., Ilver D., Caulkins C.M., Connors J.B., Dunn B.E.
    Infect. Immun. 64:905-912(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  12. "Nickel-responsive induction of urease expression in Helicobacter pylori is mediated at the transcriptional level."
    van Vliet A.H.M., Kuipers E.J., Waidner B., Davies B.J., de Vries N., Penn C.W., Vandenbroucke-Grauls C.M.J.E., Kist M., Bereswill S., Kusters J.G.
    Infect. Immun. 69:4891-4897(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  13. "Bacterial factors that mediate colonization of the stomach and virulence of Helicobacter pylori."
    Clyne M., Dolan B., Reeves E.P.
    FEMS Microbiol. Lett. 268:135-143(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON VIRULENCE OF H.PYLORI.
  14. "Supramolecular assembly and acid resistance of Helicobacter pylori urease."
    Ha N.-C., Oh S.-T., Sung J.Y., Cha K.A., Lee M.H., Oh B.-H.
    Nat. Struct. Biol. 8:505-509(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS), SUBUNIT STRUCTURE, PH DEPENDENCE.

Entry informationi

Entry nameiURE23_HELPY
AccessioniPrimary (citable) accession number: P14916
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: May 1, 1992
Last modified: September 3, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The novel dodecameric structure of the enzyme may allow it to remain active at the cell surface at acidic gastric pH. Within this dodecameric structure the 12 active sites are clustered within the interior of the proteinaceous shell. This may allow a high local concentration of ammonia within the enzyme which may protect the nickel-chelating groups from protonation.

Caution

The orthologous protein is known as the gamma/beta subunit (UreAB) in most other bacteria.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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