Reviewed, UniProtKB/Swiss-Prot P14913 (4CL2_PETCR)

Names and origin

Protein names

Recommended name:
    4-coumarate--CoA ligase 1
      Short name=4CL 1
    EC=6.2.1.12
Alternative name(s):
    4-coumaroyl-CoA synthase 1

Gene names

Name:	4CL2
Synonyms:	4CL-2

Organism

Petroselinum crispum (Parsley) (Petroselinum hortense)

Taxonomic identifier

4043 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Apiales › Apiaceae › Apioideae › apioid superclade › Apium clade › Petroselinum

Protein attributes

Sequence length	544 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Pathway	Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from 4-coumaric acid: step 1/2.
Induction	By fungal elicitor and UV irradiation.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 544	544	4-coumarate--CoA ligase 1		PRO_0000193034

Sequences

	Sequence		Length	Mass (Da)
	P14913-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: B477965C68F8C534	FASTA	544	59,783
	10 20 30 40 50 60 MGDCVAPKED LIFRSKLPDI YIPKHLPLHT YCFENISKVG DKSCLINGAT GETFTYSQVE 70 80 90 100 110 120 LLSRKVASGL NKLGIQQGDT IMLLLPNSPE YFFAFLGASY RGAISTMANP FFTSAEVIKQ 130 140 150 160 170 180 LKASLAKLII TQACYVDKVK DYAAEKNIQI ICIDDAPQDC LHFSKLMEAD ESEMPEVVID 190 200 210 220 230 240 SDDVVALPYS SGTTGLPKGV MLTHKGLVTS VAQQVDGDNP NLYMHSEDVM ICILPLFHIY 250 260 270 280 290 300 SLNAVLCCGL RAGVTILIMQ KFDIVPFLEL IQKYKVTIGP FVPPIVLAIA KSPVVDKYDL 310 320 330 340 350 360 SSVRTVMSGA APLGKELEDA VRAKFPNAKL GQGYGMTEAG PVLAMCLAFA KEPYEIKSGA 370 380 390 400 410 420 CGTVVRNAEM KIVDPETNAS LPRNQRGEIC IRGDQIMKGY LNDPESTRTT IDEEGWLHTG 430 440 450 460 470 480 DIGFIDDDDE LFIVDRLKEI IKYKGFQVAP AELEALLLTH PTISDAAVVP MIDEKAGEVP 490 500 510 520 530 540 VAFVVRTNGF TTTEEEIKQF VSKQVVFYKR IFRVFFVDAI PKSPSGKILR KDLRAKIASG DLPK

References

[1]	"Primary structures and catalytic properties of isoenzymes encoded by the two 4-coumarate:CoA ligase genes in parsley." Lozoya E., Hoffmann H., Douglas C., Schulz W., Scheel D., Hahlbrock K. Eur. J. Biochem. 176:661-667(1988) [PubMed: 3169018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Structure and elicitor or U.V.-light-stimulated expression of two 4-coumarate:CoA ligase genes in parsley." Douglas C., Hoffmann H., Schulz W., Hahlbrock K. EMBO J. 6:1189-1195(1987) [PubMed: 16453765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1-8.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X13325 mRNA. Translation: CAA31697.1. X05351 Genomic DNA. Translation: CAA28960.1.
PIR	S15695.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.2.1.12. 2662.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CL2_PETCR

Accession

Primary (citable) accession number: P14913

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)