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Protein

Mitochondrial transcription factor 1

Gene

MTF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitochondrial transcription factor that confers selective promoter recognition on the core subunit of the yeast mitochondrial RNA polymerase. Interacts with DNA in a non-specific manner.

Miscellaneous

Present with 9380 molecules/cell in log phase SD medium.1 Publication
Although strongly related to dimethyladenosine transferase proteins, it lacks the methyltransferase activity. Dimethyladenosine transferase methylates the 2 adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 12S mitochondrial rRNA in most species. This explains why 12S rRNA is not methylated in S.cerevisiae.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei23S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei77S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei101S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei137S-adenosyl-L-methioninePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • transcription initiation from mitochondrial promoter Source: SGD

Keywordsi

Molecular functionDNA-binding, Methyltransferase, RNA-binding, Transferase
Biological processTranscription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-32909-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial transcription factor 1 (EC:2.1.1.-)
Alternative name(s):
Mitochondrial transcription factor mtTFB
Mitochondrial-specificity factor
RF1023
Gene namesi
Name:MTF1
Ordered Locus Names:YMR228W
ORF Names:YM9959.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR228W
SGDiS000004841 MTF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000966212 – 341Mitochondrial transcription factor 1Add BLAST340

Proteomic databases

MaxQBiP14908
PaxDbiP14908
PRIDEiP14908

PTM databases

iPTMnetiP14908

Interactioni

Protein-protein interaction databases

BioGridi35406, 29 interactors
DIPiDIP-1542N
IntActiP14908, 1 interactor
MINTiP14908
STRINGi4932.YMR228W

Structurei

Secondary structure

1341
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi17 – 19Combined sources3
Helixi26 – 36Combined sources11
Helixi38 – 40Combined sources3
Turni45 – 47Combined sources3
Beta strandi49 – 54Combined sources6
Helixi59 – 68Combined sources10
Beta strandi71 – 76Combined sources6
Helixi80 – 89Combined sources10
Turni90 – 92Combined sources3
Beta strandi96 – 98Combined sources3
Helixi105 – 111Combined sources7
Turni112 – 115Combined sources4
Beta strandi127 – 137Combined sources11
Helixi143 – 156Combined sources14
Helixi159 – 163Combined sources5
Beta strandi164 – 173Combined sources10
Helixi174 – 181Combined sources8
Helixi191 – 199Combined sources9
Beta strandi200 – 208Combined sources9
Helixi210 – 215Combined sources6
Helixi218 – 224Combined sources7
Helixi231 – 233Combined sources3
Beta strandi234 – 236Combined sources3
Beta strandi242 – 249Combined sources8
Helixi256 – 267Combined sources12
Turni268 – 271Combined sources4
Turni274 – 276Combined sources3
Helixi277 – 280Combined sources4
Helixi285 – 289Combined sources5
Turni290 – 292Combined sources3
Turni297 – 299Combined sources3
Helixi303 – 305Combined sources3
Helixi308 – 319Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I4WX-ray2.60A2-341[»]
ProteinModelPortaliP14908
SMRiP14908
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14908

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi157 – 196Arg/Lys-rich (basic)Add BLAST40
Compositional biasi209 – 233Asp/Glu-rich (acidic)Add BLAST25
Compositional biasi246 – 260Asp/Glu-rich (acidic)Add BLAST15

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000074718
InParanoidiP14908
KOiK15267
OMAiCKLSVIA
OrthoDBiEOG092C25UQ

Family and domain databases

Gene3Di1.10.8.100, 1 hit
InterProiView protein in InterPro
IPR001737 KsgA/Erm
IPR016586 Mtf1
IPR023165 rRNA_Ade_diMease-like
IPR029063 SAM-dependent_MTases
PANTHERiPTHR11727 PTHR11727, 1 hit
PfamiView protein in Pfam
PF00398 RrnaAD, 1 hit
PIRSFiPIRSF011649 MtTFB, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51689 SAM_RNA_A_N6_MT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14908-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVPIPGIKD ISKLKFFYGF KYLWNPTVYN KIFDKLDLTK TYKHPEELKV
60 70 80 90 100
LDLYPGVGIQ SAIFYNKYCP RQYSLLEKRS SLYKFLNAKF EGSPLQILKR
110 120 130 140 150
DPYDWSTYSN LIDEERIFVP EVQSSDHIND KFLTVANVTG EGSEGLIMQW
160 170 180 190 200
LSCIGNKNWL YRFGKVKMLL WMPSTTARKL LARPGMHSRS KCSVVREAFT
210 220 230 240 250
DTKLIAISDA NELKGFDSQC IEEWDPILFS AAEIWPTKGK PIALVEMDPI
260 270 280 290 300
DFDFDVDNWD YVTRHLMILK RTPLNTVMDS LGHGGQQYFN SRITDKDLLK
310 320 330 340
KCPIDLTNDE FIYLTKLFME WPFKPDILMD FVDMYQTEHS G
Length:341
Mass (Da):39,727
Last modified:January 23, 2007 - v4
Checksum:iB579726EE318B0B7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79R → S in CAA31864 (PubMed:2853292).Curated1
Sequence conflicti287 – 288QY → PI in CAA31864 (PubMed:2853292).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13513 Genomic DNA Translation: CAA31864.1
Z49939 Genomic DNA Translation: CAA90199.1
BK006946 Genomic DNA Translation: DAA10127.1
PIRiS57595
RefSeqiNP_013955.1, NM_001182735.1

Genome annotation databases

EnsemblFungiiYMR228W; YMR228W; YMR228W
GeneIDi855268
KEGGisce:YMR228W

Similar proteinsi

Entry informationi

Entry nameiMTF1_YEAST
AccessioniPrimary (citable) accession number: P14908
Secondary accession number(s): D6W053
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 162 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names
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