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Reviewed, UniProtKB/Swiss-Prot P14899 (AMY3_DICT6)

Last modified November 3, 2009. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alpha-amylase 3
    EC=3.2.1.1
Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase
Gene names
Name: amyC
Ordered Locus Names: DICTH_1568
OrganismDictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) [Complete proteome] [HAMAP]
Taxonomic identifier309799 [NCBI]
Taxonomic lineageBacteria › Dictyoglomi › Dictyoglomales › Dictyoglomaceae › Dictyoglomus

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Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm.

Miscellaneous

The profile of production of reducing sugar from soluble starch by the action of amyC was intermediate between those of amyA and amyB.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

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Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentCytoplasm
   LigandCalcium
Metal-binding
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionalpha-amylase activity

Inferred from electronic annotation. Source: EC

calcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499Alpha-amylase 3
PRO_0000054286

Sites

Active site2131Nucleophile By similarity
Active site2481Proton donor By similarity
Active site3151 By similarity
Metal binding1271Calcium By similarity
Metal binding1831Calcium By similarity
Metal binding2171Calcium; via carbonyl oxygen By similarity

Experimental info

Sequence conflict279 – 29618VANQK…EFERE → SSKSKRLRNYRISRLKR in CAA34072. Ref.1
Sequence conflict341 – 3444PGIP → AGNT in CAA34072. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P14899-1 [UniParc].

Last modified January 20, 2009. Version 2.
Checksum: FE6E3FC8F5D0CDAB

FASTA49959,086
        10         20         30         40         50         60 
MKIKFFIKRT LIFIFILVTF LTYIHGYNEP WYKNAIFYEV FVRSFADSDG DRVGDLNGLI 

        70         80         90        100        110        120 
DKLDYFKNLN ITALWLMPIF PSVSYHGYDV TDYYDIHPGY GTMEDFENLI RKAHEKNIKI 

       130        140        150        160        170        180 
ILDLVVNHTS SRHPWFVSSA SSYNSPYRDY YIWSTEKPEK NSNLWYKKPT GYYYALFWSE 

       190        200        210        220        230        240 
MPDLNFDNPK VREEVKKIAK FWIEKGVDGF RLDAAKHIYD DDSKNIQWWK EFYSYLKSIK 

       250        260        270        280        290        300 
PDVYLVGEVW DNEYKIAEYY KGLPSNFNFP LSDKIMNSVA NQKDLGIIEF LEFERELFGE 

       310        320        330        340        350        360 
NNTDFADAIF LRNHDQVRVR TFFGGSIDKS ILAGSIYLTL PGIPFIYYGE EIGMEGSKPD 

       370        380        390        400        410        420 
EYIREPFKWT DDMKSKYQTY WIIPRYNLPG NGIALDTEEK DPNSIYNHYK KLLEIRVKCR 

       430        440        450        460        470        480 
ALSNGKIERI KTQDRSILAY KLELEDEKIM VVHNLNRIEN TFNFNNEIKE KDILYIRNAK 

       490 
TEKNKIILGP YSTVIVKIP

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References

« Hide 'large scale' references
[1]"Cloning and expression in Escherichia coli of two additional amylase genes of a strictly anaerobic thermophile, Dictyoglomus thermophilum, and their nucleotide sequences with extremely low guanine-plus-cytosine contents."
Horinouchi S., Fukusumi S., Ohshima T., Beppu T.
Eur. J. Biochem. 176:243-253(1988) [PubMed: 2458257] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete genome sequence of Dictyoglomus thermophilum strain ATCC 35947 / DSM 3960 / H-6-12."
Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G.
Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

X15948 Genomic DNA. Translation: CAA34072.1.
CP001146 Genomic DNA. Translation: ACI19039.1.
PIRS01313.
RefSeqYP_002251382.1.

3D structure databases

HSSPHSSP built from PDB template 1LWJ based on UniProtKB P80099.
ModBaseSearch...

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Genome annotation databases

GeneID6945329.
GenomeReviewsGene locus DICTH_1568 in contig CP001146_GR.
KEGGdth:DICTH_1568.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAPGHESAN.

Enzyme and pathway databases

BRENDA3.2.1.1. 141232.

Family and domain databases

InterProIPR006046. Glyco_hydro_13.
[Graphical view]
PRINTSPR00110. ALPHAAMYLASE.
ProtoNetSearch...

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Entry information

Entry nameAMY3_DICT6
AccessionPrimary (citable) accession number: P14899
Secondary accession number(s): B5YAB2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 20, 2009
Last modified: November 3, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents