P14898 (AMY2_DICT6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-amylase 2
EC=3.2.1.1

Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase

Gene names

Name:	amyB
Ordered Locus Names:	DICTH_0636.1

Organism

Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) [Complete proteome] [HAMAP]

Taxonomic identifier

309799 [NCBI]

Taxonomic lineage

Bacteria › Dictyoglomi › Dictyoglomales › Dictyoglomaceae › Dictyoglomus

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.
Cofactor	Binds 1 calcium ion per subunit Potential.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	When compared to AmyA, AmyB produced larger amounts of reducing sugar.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Calcium Metal-binding
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	Alpha-amylase 2		PRO_0000054285
Sites
	Active site	309	1	Nucleophile By similarity
	Active site	338	1	Proton donor By similarity
	Metal binding	236	1	Calcium Potential
	Site	404	1	Transition state stabilizer By similarity
Experimental info
	Sequence conflict	124	1	N → S in CAA31586. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P14898 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: 368FF33D155FC3F4	FASTA	562	67,027
	10 20 30 40 50 60 MIYDDKIFGD LCHKEFLVER EVKKLEEIYL EEVLPEDPKP EDEIEFTFNC PLKFHITSGK 70 80 90 100 110 120 IVKDNREIYT FNIQERKTQW NDSIFNFSEI IKIKIPPLKE NGLYQIHLYE MNEKIYEQYL 130 140 150 160 170 180 SIDNFEAPLW SEESIIYHIF IDRFAKDEKE VEYSENLKEK LGGNLKGILS RLDYIENLGI 190 200 210 220 230 240 NTIWISPIFK STSYHGYDIE DYFEIDPIWG TKEDLKKLVR EAFNRGIRII LDFVPNHMSY 250 260 270 280 290 300 KNPIFQKALK DKNSNLRSWF IFKGEDYETF FGVKSMPKIN LKNKEAIDYI INAAKYWIRE 310 320 330 340 350 360 FGISGYRMDH ATGPDINFWS IFYYNLKSEF PETFYFGEIV ETPKETKKYV GKFDGTLDFY 370 380 390 400 410 420 LFKIIRDFFI GKRWSTKEFV KMIDLEEKFY GNKFKRISFL ENHDSNRFLW VAKDKKLLRL 430 440 450 460 470 480 ASIFQFSINA IPIIYNGQEM GCSQYRDILE GNRTLHEHAR LPIPWSDDKQ DKELIDFYRQ 490 500 510 520 530 540 LVKIRKSHPA LYKGTFIPIF SDMISFIKET QEESILVLIN IEDKEEIFNL NGTYRDLFSG 550 560 NIYTNSLKLG PMSAHLLLRI DH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression in Escherichia coli of two additional amylase genes of a strictly anaerobic thermophile, Dictyoglomus thermophilum, and their nucleotide sequences with extremely low guanine-plus-cytosine contents." Horinouchi S., Fukusumi S., Ohshima T., Beppu T. Eur. J. Biochem. 176:243-253(1988) [PubMed: 2458257] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-8.
[2]	"The complete genome sequence of Dictyoglomus thermophilum strain ATCC 35947 / DSM 3960 / H-6-12." Dodson R.J., Durkin A.S., Wu M., Eisen J., Sutton G. Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35947 / DSM 3960 / H-6-12.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13199 Genomic DNA. Translation: CAA31586.1. CP001146 Genomic DNA. No translation available.
PIR	S01312.
3D structure databases
ProteinModelPortal	P14898.
ModBase	Search...
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus DICTH_0636.1 in contig CP001146_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG465660.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10357. Alpha_amylase. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AMY2_DICT6

Accession

Primary (citable) accession number: P14898

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 20, 2009
Last modified:	December 14, 2011
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program