P14892 (CWLA_BACSP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetylmuramoyl-L-alanine amidase CwlA
EC=3.5.1.28

Alternative name(s):
Autolysin
Cell wall hydrolase

Gene names

Name:

cwlA

Organism

Bacillus sp.

Taxonomic identifier

1409 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted Potential.
Miscellaneous	Retention of enzymatic activity was limited to the N-terminal fragment (AA 1-196).
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Competence Sporulation
Cellular component	Secreted
Domain	Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Hydrolase
Gene Ontology (GO)
Biological_process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW establishment of competence for transformation Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 37	37
	Chain	38 – 251	214	N-acetylmuramoyl-L-alanine amidase CwlA		PRO_0000006452

Sequences

	Sequence		Length	Mass (Da)
	P14892 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 1F52F36B20576F0D	FASTA	251	28,473
	10 20 30 40 50 60 MEIKQMLVPV SRYSVLCPYE MNPTEITFHN TYNDAPAINE RNNVANNSTG TSFHIAVDDK 70 80 90 100 110 120 EAIQLIPFNR NAWHAGDGTN GRGNRHSIGV EICYSQSGGA RYRKAELNAV EVIAQLMIQF 130 140 150 160 170 180 DIPISKVKTH QERNGKYCPH RMLDEGRVQW FKNQCANRAS SIKNSNKTQE TGKVEIIVNK 190 200 210 220 230 240 FNKVVTYEFG TALVPEMLGM MDALGYESRI ISYGDKQGLV RFETAYRQGN ELDKATAWLD 250 AKGLKYFYTK E

References

[1]	"Cloning, sequencing and expression of a Bacillus bacteriolytic enzyme in Escherichia coli." Potvin C., Leclerc D., Tremblay G., Asselin A., Bellemare G. Mol. Gen. Genet. 214:241-248(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13524 Genomic DNA. Translation: CAA31877.1.
PIR	S08306.
3D structure databases
ProteinModelPortal	P14892.
SMR	P14892. Positions 1-147.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.80.10. 1 hit.
InterPro	IPR021976. Amidase02_C. IPR002502. Amidase_domain. [Graphical view]
Pfam	PF12123. Amidase02_C. 1 hit. PF01510. Amidase_2. 1 hit. [Graphical view]
SMART	SM00644. Ami_2. 1 hit. [Graphical view]
SUPFAM	SSF55846. Amidase_2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CWLA_BACSP

Accession

Primary (citable) accession number: P14892

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	April 3, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program