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Protein

Beta-crystallin A3

Gene

Cryba1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Crystallins are the dominant structural components of the vertebrate eye lens.

GO - Molecular functioni

GO - Biological processi

  • lens development in camera-type eye Source: RGD
  • negative regulation of cytokine production Source: RGD
  • negative regulation of ERK1 and ERK2 cascade Source: RGD
  • negative regulation of phosphatidylinositol 3-kinase signaling Source: RGD
  • negative regulation of protein kinase B signaling Source: RGD
  • negative regulation of TOR signaling Source: RGD
  • positive regulation of anoikis Source: RGD
  • visual perception Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Eye lens protein

Names & Taxonomyi

Protein namesi
Gene namesi
Name:Cryba1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi2415. Cryba1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 215215Beta-crystallin A3PRO_0000006333Add
BLAST
Chaini23 – 215193Beta-crystallin A3, isoform A1, Delta4 formPRO_0000226695Add
BLAST
Chaini26 – 215190Beta-crystallin A3, isoform A1, Delta7 formPRO_0000226696Add
BLAST
Chaini27 – 215189Beta-crystallin A3, isoform A1, Delta8 formPRO_0000226697Add
BLAST
Isoform A1 (identifier: P14881-2)
Initiator methionineiRemovedBy similarity

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei82 – 821S-glutathionyl cysteine; alternateBy similarity
Modified residuei82 – 821S-methylcysteine; alternateBy similarity
Modified residuei117 – 1171S-glutathionyl cysteine; alternateBy similarity
Modified residuei117 – 1171S-methylcysteine; alternateBy similarity
Modified residuei137 – 1371Omega-N-methylated arginineBy similarity
Isoform A1 (identifier: P14881-2)
Modified residuei2 – 21N-acetylalanine By similarityBy similarity

Post-translational modificationi

Specific cleavages in the N-terminal arm occur during lens maturation and give rise to several truncated forms.By similarity
The initiator methionine for isoform A1 is removed. The new N-terminal amino acid is then N-acetylated (By similarity).By similarity

Keywords - PTMi

Acetylation, Glutathionylation, Methylation

Proteomic databases

PaxDbiP14881.
PRIDEiP14881.

PTM databases

PhosphoSiteiP14881.

Miscellaneous databases

PMAP-CutDBP14881.

Expressioni

Gene expression databases

GenevisibleiP14881. RN.

Interactioni

Subunit structurei

Homo/heterodimer, or complexes of higher-order. The structure of beta-crystallin oligomers seems to be stabilized through interactions between the N-terminal arms (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011608.

Structurei

3D structure databases

ProteinModelPortaliP14881.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 7040Beta/gamma crystallin 'Greek key' 1PROSITE-ProRule annotationAdd
BLAST
Domaini71 – 11747Beta/gamma crystallin 'Greek key' 2PROSITE-ProRule annotationAdd
BLAST
Domaini124 – 16542Beta/gamma crystallin 'Greek key' 3PROSITE-ProRule annotationAdd
BLAST
Domaini166 – 21449Beta/gamma crystallin 'Greek key' 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3030N-terminal armAdd
BLAST
Regioni118 – 1236Connecting peptide

Domaini

Has a two-domain beta-structure, folded into four very similar Greek key motifs.

Sequence similaritiesi

Belongs to the beta/gamma-crystallin family.Curated
Contains 4 beta/gamma crystallin 'Greek key' domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IGDN. Eukaryota.
ENOG410ZGPB. LUCA.
GeneTreeiENSGT00760000118812.
HOGENOMiHOG000234388.
HOVERGENiHBG003364.
InParanoidiP14881.
OMAiSLGPWKI.
OrthoDBiEOG091G0JYH.
PhylomeDBiP14881.
TreeFamiTF331401.

Family and domain databases

InterProiIPR001064. Beta/gamma_crystallin.
IPR011024. G_crystallin-rel.
[Graphical view]
PfamiPF00030. Crystall. 2 hits.
[Graphical view]
PRINTSiPR01367. BGCRYSTALLIN.
SMARTiSM00247. XTALbg. 2 hits.
[Graphical view]
SUPFAMiSSF49695. SSF49695. 1 hit.
PROSITEiPS50915. CRYSTALLIN_BETA_GAMMA. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform A3 (identifier: P14881-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METQTVQREL ETLPTTKMAQ TNPMPGSMGP WKITIYDQEN FQGKRMEFTS
60 70 80 90 100
SCPNVSERSF DNVRSLKVEC GAWIGYEHTS FCGQQFILER GEYPRWDAWS
110 120 130 140 150
GSNAYHIERL MSFRPICSAN HKESKITIFE KENFIGRQWE ICDDYPSLQA
160 170 180 190 200
MGWFNNEVGS MKIQCGAWVC YQYPGYRGYQ YILECDHHGG DYKHWREWGT
210
HAQTSQIQSI RRIQQ
Length:215
Mass (Da):25,270
Last modified:February 1, 2005 - v2
Checksum:i3842A801352A96C3
GO
Isoform A1 (identifier: P14881-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: Missing.

Show »
Length:198
Mass (Da):23,283
Checksum:i0A41FC37097CE99D
GO

Mass spectrometryi

Molecular mass is 25308.9 Da from positions 1 - 215. Determined by ESI. Isoform A3.1 Publication
Molecular mass is 23191.3 Da from positions 19 - 215. Determined by ESI. Isoform A1.1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1717Missing in isoform A1. CuratedVSP_018711Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013248 mRNA. Translation: AAB67118.1.
X15143 mRNA. Translation: CAA33241.1.
RefSeqiNP_037188.1. NM_013056.1. [P14881-1]
UniGeneiRn.20326.

Genome annotation databases

EnsembliENSRNOT00000011608; ENSRNOP00000011608; ENSRNOG00000008700. [P14881-1]
GeneIDi25583.
KEGGirno:25583.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013248 mRNA. Translation: AAB67118.1.
X15143 mRNA. Translation: CAA33241.1.
RefSeqiNP_037188.1. NM_013056.1. [P14881-1]
UniGeneiRn.20326.

3D structure databases

ProteinModelPortaliP14881.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011608.

PTM databases

PhosphoSiteiP14881.

Proteomic databases

PaxDbiP14881.
PRIDEiP14881.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011608; ENSRNOP00000011608; ENSRNOG00000008700. [P14881-1]
GeneIDi25583.
KEGGirno:25583.

Organism-specific databases

CTDi1411.
RGDi2415. Cryba1.

Phylogenomic databases

eggNOGiENOG410IGDN. Eukaryota.
ENOG410ZGPB. LUCA.
GeneTreeiENSGT00760000118812.
HOGENOMiHOG000234388.
HOVERGENiHBG003364.
InParanoidiP14881.
OMAiSLGPWKI.
OrthoDBiEOG091G0JYH.
PhylomeDBiP14881.
TreeFamiTF331401.

Miscellaneous databases

PMAP-CutDBP14881.
PROiP14881.

Gene expression databases

GenevisibleiP14881. RN.

Family and domain databases

InterProiIPR001064. Beta/gamma_crystallin.
IPR011024. G_crystallin-rel.
[Graphical view]
PfamiPF00030. Crystall. 2 hits.
[Graphical view]
PRINTSiPR01367. BGCRYSTALLIN.
SMARTiSM00247. XTALbg. 2 hits.
[Graphical view]
SUPFAMiSSF49695. SSF49695. 1 hit.
PROSITEiPS50915. CRYSTALLIN_BETA_GAMMA. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRBA1_RAT
AccessioniPrimary (citable) accession number: P14881
Secondary accession number(s): O35237
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: February 1, 2005
Last modified: September 7, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.