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P14874 (ILVB2_BRANA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acetolactate synthase 2, chloroplastic

EC=2.2.1.6
Alternative name(s):
ALS II
Acetohydroxy-acid synthase II
Acetolactate synthase II
OrganismBrassica napus (Rape)
Taxonomic identifier3708 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Protein attributes

Sequence length637 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

2 pyruvate = 2-acetolactate + CO2.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.

Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.

Subcellular location

Plastidchloroplast.

Miscellaneous

Acetolactate synthase is the target enzyme for sulfonylurea and imidazolinone herbicides.

Sequence similarities

Belongs to the TPP enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 7373Chloroplast By similarity
Chain74 – 637564Acetolactate synthase 2, chloroplastic
PRO_0000035657

Regions

Nucleotide binding329 – 35022FAD By similarity
Nucleotide binding372 – 39120FAD By similarity
Region462 – 54281Thiamine pyrophosphate binding

Sites

Metal binding5131Magnesium By similarity
Metal binding5401Magnesium By similarity
Binding site1201Thiamine pyrophosphate By similarity
Binding site2221FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
P14874 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 9FC6A6E8124C2455

FASTA63769,970
        10         20         30         40         50         60 
MASFSFFGTI PSSPTKASVF SLPVSVTTLP SFPRRRATRV SVSANSKKDQ DRTASRRENP 

        70         80         90        100        110        120 
STFSSKYAPN VPRSGADILV EALERQGVDV VFAYPGGASM EIHQALTRSN TIRNVLPRHE 

       130        140        150        160        170        180 
QGGIFAAEGY ARSSGKPGIC IATSGPGAMN LVSGLADALF DSVPLIAITG QVPRRMIGTM 

       190        200        210        220        230        240 
AFQETPVVEV TRTITKHNYL VMEVDDIPRI VREAFFLATS VRPGPVLIDV PKDVQQQFAI 

       250        260        270        280        290        300 
PNWEQPMRLP LYMSTMPKPP KVSHLEQILR LVSESKRPVL YVGGGCLNSS EELRRFVELT 

       310        320        330        340        350        360 
GIPVASTFMG LGSYPCDDEE FSLQMLGMHG TVYANYAVEY SDLLLAFGVR FDDRVTGKLE 

       370        380        390        400        410        420 
AFASRAKIVH IDIDSTEIGK NKTPHVSVCC DVQLALQGMN EVLENRRDVL DFGEWRCELN 

       430        440        450        460        470        480 
EQRLKFPLRY KTFGEEIPPQ YAIQLLDELT DGKAIITTGV GQHQMWAAQF YRFKKPRQWL 

       490        500        510        520        530        540 
SSGGLGAMGF GLPAAMGAAI ANPGAVVVDI DGDGSFIMNI QELATIRVEN LPVKVLLINN 

       550        560        570        580        590        600 
QHLGMVLQWE DHFYAANRAD SFLGDPANPE AVFPDMLLFA ASCGIPAARV TRREDLREAI 

       610        620        630 
QTMLDTPGPF LLDVVCPHQD HVLPLIPSGG TFKDIIV 

« Hide

References

[1]"Molecular characterization and genetic origin of the Brassica napus acetohydroxyacid synthase multigene family."
Rutledge R.G., Ouellet T., Hattori J., Miki B.L.A.
Mol. Gen. Genet. 229:31-40(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Topas.
[2]"Isolation, expression and phylogenetic inheritance of an acetolactate synthase gene from Brassica napus."
Wiersma P.A., Schmiemann M.G., Condie J.A., Crosby W.L., Moloney M.M.
Mol. Gen. Genet. 219:413-420(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Westar.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z11525 Genomic DNA. Translation: CAA77614.1.
X16708 Genomic DNA. Translation: CAA34680.1.
PIRYCRP. JQ0357.

3D structure databases

ProteinModelPortalP14874.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00047; UER00055.
UPA00049; UER00059.

Family and domain databases

Gene3D3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProIPR012846. Acetolactate_synth_lsu.
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
SUPFAMSSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.
TIGRFAMsTIGR00118. acolac_lg. 1 hit.
PROSITEPS00187. TPP_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameILVB2_BRANA
AccessionPrimary (citable) accession number: P14874
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 11, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways