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P14874

- ILVB2_BRANA

UniProt

P14874 - ILVB2_BRANA

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Protein

Acetolactate synthase 2, chloroplastic

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

2 pyruvate = 2-acetolactate + CO2.

Cofactori

Binds 1 magnesium ion per subunit By similarity.
Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei120 – 1201Thiamine pyrophosphate By similarity
Binding sitei222 – 2221FAD By similarity
Metal bindingi513 – 5131Magnesium By similarity
Metal bindingi540 – 5401Magnesium By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi329 – 35022FAD By similarityAdd
BLAST
Nucleotide bindingi372 – 39120FAD By similarityAdd
BLAST

GO - Molecular functioni

  1. acetolactate synthase activity Source: UniProtKB-EC
  2. flavin adenine dinucleotide binding Source: InterPro
  3. magnesium ion binding Source: InterPro
  4. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. isoleucine biosynthetic process Source: UniProtKB-UniPathway
  2. response to herbicide Source: UniProtKB-KW
  3. valine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Herbicide resistance

Keywords - Ligandi

FAD, Flavoprotein, Magnesium, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

UniPathwayiUPA00047; UER00055.
UPA00049; UER00059.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase 2, chloroplastic (EC:2.2.1.6)
Alternative name(s):
ALS II
Acetohydroxy-acid synthase II
Acetolactate synthase II
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 7373Chloroplast By similarityAdd
BLAST
Chaini74 – 637564Acetolactate synthase 2, chloroplasticPRO_0000035657Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP14874.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni462 – 54281Thiamine pyrophosphate bindingAdd
BLAST

Sequence similaritiesi

Belongs to the TPP enzyme family.

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR012846. Acetolactate_synth_lsu.
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00118. acolac_lg. 1 hit.
PROSITEiPS00187. TPP_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14874-1 [UniParc]FASTAAdd to Basket

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MASFSFFGTI PSSPTKASVF SLPVSVTTLP SFPRRRATRV SVSANSKKDQ    50
DRTASRRENP STFSSKYAPN VPRSGADILV EALERQGVDV VFAYPGGASM 100
EIHQALTRSN TIRNVLPRHE QGGIFAAEGY ARSSGKPGIC IATSGPGAMN 150
LVSGLADALF DSVPLIAITG QVPRRMIGTM AFQETPVVEV TRTITKHNYL 200
VMEVDDIPRI VREAFFLATS VRPGPVLIDV PKDVQQQFAI PNWEQPMRLP 250
LYMSTMPKPP KVSHLEQILR LVSESKRPVL YVGGGCLNSS EELRRFVELT 300
GIPVASTFMG LGSYPCDDEE FSLQMLGMHG TVYANYAVEY SDLLLAFGVR 350
FDDRVTGKLE AFASRAKIVH IDIDSTEIGK NKTPHVSVCC DVQLALQGMN 400
EVLENRRDVL DFGEWRCELN EQRLKFPLRY KTFGEEIPPQ YAIQLLDELT 450
DGKAIITTGV GQHQMWAAQF YRFKKPRQWL SSGGLGAMGF GLPAAMGAAI 500
ANPGAVVVDI DGDGSFIMNI QELATIRVEN LPVKVLLINN QHLGMVLQWE 550
DHFYAANRAD SFLGDPANPE AVFPDMLLFA ASCGIPAARV TRREDLREAI 600
QTMLDTPGPF LLDVVCPHQD HVLPLIPSGG TFKDIIV 637
Length:637
Mass (Da):69,970
Last modified:April 1, 1990 - v1
Checksum:i9FC6A6E8124C2455
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11525 Genomic DNA. Translation: CAA77614.1.
X16708 Genomic DNA. Translation: CAA34680.1.
PIRiJQ0357. YCRP.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11525 Genomic DNA. Translation: CAA77614.1 .
X16708 Genomic DNA. Translation: CAA34680.1 .
PIRi JQ0357. YCRP.

3D structure databases

ProteinModelPortali P14874.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00047 ; UER00055 .
UPA00049 ; UER00059 .

Family and domain databases

Gene3Di 3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProi IPR012846. Acetolactate_synth_lsu.
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR011766. TPP_enzyme-bd_C.
[Graphical view ]
Pfami PF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
SUPFAMi SSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00118. acolac_lg. 1 hit.
PROSITEi PS00187. TPP_ENZYMES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization and genetic origin of the Brassica napus acetohydroxyacid synthase multigene family."
    Rutledge R.G., Ouellet T., Hattori J., Miki B.L.A.
    Mol. Gen. Genet. 229:31-40(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Topas.
  2. "Isolation, expression and phylogenetic inheritance of an acetolactate synthase gene from Brassica napus."
    Wiersma P.A., Schmiemann M.G., Condie J.A., Crosby W.L., Moloney M.M.
    Mol. Gen. Genet. 219:413-420(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Westar.
    Tissue: Leaf.

Entry informationi

Entry nameiILVB2_BRANA
AccessioniPrimary (citable) accession number: P14874
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 11, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Acetolactate synthase is the target enzyme for sulfonylurea and imidazolinone herbicides.

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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