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P14874

- ILVB2_BRANA

UniProt

P14874 - ILVB2_BRANA

Protein

Acetolactate synthase 2, chloroplastic

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    2 pyruvate = 2-acetolactate + CO2.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 thiamine pyrophosphate per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei120 – 1201Thiamine pyrophosphateBy similarity
    Binding sitei222 – 2221FADBy similarity
    Metal bindingi513 – 5131MagnesiumBy similarity
    Metal bindingi540 – 5401MagnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi329 – 35022FADBy similarityAdd
    BLAST
    Nucleotide bindingi372 – 39120FADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. acetolactate synthase activity Source: UniProtKB-EC
    2. flavin adenine dinucleotide binding Source: InterPro
    3. magnesium ion binding Source: InterPro
    4. thiamine pyrophosphate binding Source: InterPro

    GO - Biological processi

    1. isoleucine biosynthetic process Source: UniProtKB-UniPathway
    2. response to herbicide Source: UniProtKB-KW
    3. valine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Herbicide resistance

    Keywords - Ligandi

    FAD, Flavoprotein, Magnesium, Metal-binding, Thiamine pyrophosphate

    Enzyme and pathway databases

    UniPathwayiUPA00047; UER00055.
    UPA00049; UER00059.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acetolactate synthase 2, chloroplastic (EC:2.2.1.6)
    Alternative name(s):
    ALS II
    Acetohydroxy-acid synthase II
    Acetolactate synthase II
    OrganismiBrassica napus (Rape)
    Taxonomic identifieri3708 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 7373ChloroplastBy similarityAdd
    BLAST
    Chaini74 – 637564Acetolactate synthase 2, chloroplasticPRO_0000035657Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP14874.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni462 – 54281Thiamine pyrophosphate bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TPP enzyme family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.40.50.1220. 1 hit.
    3.40.50.970. 2 hits.
    InterProiIPR012846. Acetolactate_synth_lsu.
    IPR029035. DHS-like_NAD/FAD-binding_dom.
    IPR029061. THDP-binding.
    IPR012000. Thiamin_PyroP_enz_cen_dom.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    IPR000399. TPP-bd_CS.
    IPR011766. TPP_enzyme-bd_C.
    [Graphical view]
    PfamiPF02775. TPP_enzyme_C. 1 hit.
    PF00205. TPP_enzyme_M. 1 hit.
    PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52467. SSF52467. 1 hit.
    SSF52518. SSF52518. 2 hits.
    TIGRFAMsiTIGR00118. acolac_lg. 1 hit.
    PROSITEiPS00187. TPP_ENZYMES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P14874-1 [UniParc]FASTAAdd to Basket

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    MASFSFFGTI PSSPTKASVF SLPVSVTTLP SFPRRRATRV SVSANSKKDQ    50
    DRTASRRENP STFSSKYAPN VPRSGADILV EALERQGVDV VFAYPGGASM 100
    EIHQALTRSN TIRNVLPRHE QGGIFAAEGY ARSSGKPGIC IATSGPGAMN 150
    LVSGLADALF DSVPLIAITG QVPRRMIGTM AFQETPVVEV TRTITKHNYL 200
    VMEVDDIPRI VREAFFLATS VRPGPVLIDV PKDVQQQFAI PNWEQPMRLP 250
    LYMSTMPKPP KVSHLEQILR LVSESKRPVL YVGGGCLNSS EELRRFVELT 300
    GIPVASTFMG LGSYPCDDEE FSLQMLGMHG TVYANYAVEY SDLLLAFGVR 350
    FDDRVTGKLE AFASRAKIVH IDIDSTEIGK NKTPHVSVCC DVQLALQGMN 400
    EVLENRRDVL DFGEWRCELN EQRLKFPLRY KTFGEEIPPQ YAIQLLDELT 450
    DGKAIITTGV GQHQMWAAQF YRFKKPRQWL SSGGLGAMGF GLPAAMGAAI 500
    ANPGAVVVDI DGDGSFIMNI QELATIRVEN LPVKVLLINN QHLGMVLQWE 550
    DHFYAANRAD SFLGDPANPE AVFPDMLLFA ASCGIPAARV TRREDLREAI 600
    QTMLDTPGPF LLDVVCPHQD HVLPLIPSGG TFKDIIV 637
    Length:637
    Mass (Da):69,970
    Last modified:April 1, 1990 - v1
    Checksum:i9FC6A6E8124C2455
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11525 Genomic DNA. Translation: CAA77614.1.
    X16708 Genomic DNA. Translation: CAA34680.1.
    PIRiJQ0357. YCRP.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11525 Genomic DNA. Translation: CAA77614.1 .
    X16708 Genomic DNA. Translation: CAA34680.1 .
    PIRi JQ0357. YCRP.

    3D structure databases

    ProteinModelPortali P14874.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00047 ; UER00055 .
    UPA00049 ; UER00059 .

    Family and domain databases

    Gene3Di 3.40.50.1220. 1 hit.
    3.40.50.970. 2 hits.
    InterProi IPR012846. Acetolactate_synth_lsu.
    IPR029035. DHS-like_NAD/FAD-binding_dom.
    IPR029061. THDP-binding.
    IPR012000. Thiamin_PyroP_enz_cen_dom.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    IPR000399. TPP-bd_CS.
    IPR011766. TPP_enzyme-bd_C.
    [Graphical view ]
    Pfami PF02775. TPP_enzyme_C. 1 hit.
    PF00205. TPP_enzyme_M. 1 hit.
    PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52467. SSF52467. 1 hit.
    SSF52518. SSF52518. 2 hits.
    TIGRFAMsi TIGR00118. acolac_lg. 1 hit.
    PROSITEi PS00187. TPP_ENZYMES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization and genetic origin of the Brassica napus acetohydroxyacid synthase multigene family."
      Rutledge R.G., Ouellet T., Hattori J., Miki B.L.A.
      Mol. Gen. Genet. 229:31-40(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Topas.
    2. "Isolation, expression and phylogenetic inheritance of an acetolactate synthase gene from Brassica napus."
      Wiersma P.A., Schmiemann M.G., Condie J.A., Crosby W.L., Moloney M.M.
      Mol. Gen. Genet. 219:413-420(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Westar.
      Tissue: Leaf.

    Entry informationi

    Entry nameiILVB2_BRANA
    AccessioniPrimary (citable) accession number: P14874
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Acetolactate synthase is the target enzyme for sulfonylurea and imidazolinone herbicides.

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3