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P14871

- MTF1_PLAOK

UniProt

P14871 - MTF1_PLAOK

Protein

Modification methylase FokI

Gene

fokIM

Organism
Planomicrobium okeanokoites (Planococcus okeanokoites) (Flavobacterium okeanokoites)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence 5'-GGATG-3' in one strand and 3'-CATCC-5' in the other, causes specific methylation on the A of both strands, and protects the DNA from cleavage by the FokI endonuclease. The 2 domains of the protein participates in modification of the two strands.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3405. M.FokI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase FokI (EC:2.1.1.72)
    Short name:
    M.FokI
    Alternative name(s):
    Adenine-specific methyltransferase FokI
    Gene namesi
    Name:fokIM
    Synonyms:mfoKI
    OrganismiPlanomicrobium okeanokoites (Planococcus okeanokoites) (Flavobacterium okeanokoites)
    Taxonomic identifieri244 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPlanococcaceaePlanomicrobium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 647647Modification methylase FokIPRO_0000087960Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliP14871.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di1.10.1020.10. 1 hit.
    3.40.50.150. 4 hits.
    InterProiIPR012186. Ade-mod_methylase_MStsI.
    IPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02086. MethyltransfD12. 2 hits.
    [Graphical view]
    PIRSFiPIRSF036638. M_m6A_StsI. 1 hit.
    PRINTSiPR00505. D12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 3 hits.
    TIGRFAMsiTIGR00571. dam. 1 hit.
    PROSITEiPS00092. N6_MTASE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14871-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRFIGSKVNL LDNIQEVIEE NVKDDAHVFM DLFSGTGIVG ENFKKDYQVL    50
    SNDSLYFSYI LLKAKIENNS IPNFSELKKI GIKEPLHYLE NEEFEISHEF 100
    FLTHNYSPYM GCERMYFTVE NASRIDFIRL TLNRWKNESL INELEFAYLL 150
    AILIEAVPFI SNISGTYGAY LKHWDKRALG KLKLRTLDIG NNHYANKTYN 200
    EDANSLIEKV YGDILYIDPP YNGRQYISNY HLLETIALYD YPEIYGKTGL 250
    RPYVESKSLY CQKKEVGNAF NHLIEKANFR HILVSYSSEG LLLEEEIESI 300
    LKSHGLPETY RIYKMPYRKY KSKHKQEASE LHEYIFYIQK DIALTNSVKS 350
    NKKIEVGKHK TNSYIKSPLN YVGGKHKLLN QIVPLFPDKI DTFVDLFSGG 400
    FNVGINVNAN KIIATDINTY VVEVLDTMKK TSVEEVIAHI ERRIEEYGLS 450
    KSNEEGFKAF RNYYNKTKKP LDLYTLICYS FNYQFRFNNN QEYNNPFGRE 500
    RSQFSPALKK KLVLFIEALH EKNVQFVCSE FEHFNFSQLD QNDLVYCDPP 550
    YLITTGSYND GNRGFKDWNR LQEIKLLDIL DHLNSKGVYF ALSNVLSHKG 600
    LENELLLEWS KKYNIHHLQH SYSNSSHNTT RGESQEVLIT NYTNYTK 647
    Length:647
    Mass (Da):75,626
    Last modified:April 1, 1990 - v1
    Checksum:i66F7D05F17345836
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04623 Genomic DNA. Translation: AAA24926.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04623 Genomic DNA. Translation: AAA24926.1 .

    3D structure databases

    ProteinModelPortali P14871.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3405. M.FokI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.1020.10. 1 hit.
    3.40.50.150. 4 hits.
    InterProi IPR012186. Ade-mod_methylase_MStsI.
    IPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02086. MethyltransfD12. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF036638. M_m6A_StsI. 1 hit.
    PRINTSi PR00505. D12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 3 hits.
    TIGRFAMsi TIGR00571. dam. 1 hit.
    PROSITEi PS00092. N6_MTASE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The fokI restriction-modification system. I. Organization and nucleotide sequences of the restriction and modification genes."
      Kita K., Kotani H., Sugisaki H., Takanami M.
      J. Biol. Chem. 264:5751-5756(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-9.
      Strain: ATCC 33414 / DSM 15489 / NBRC 12536 / NCIMB 561 / CIP 105082 / LMG 4030 / VKM B-1175.
    2. "The FokI restriction-modification system. II. Presence of two domains in FokI methylase responsible for modification of different DNA strands."
      Sugisaki H., Kita K., Takanami M.
      J. Biol. Chem. 264:5757-5761(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAINS.

    Entry informationi

    Entry nameiMTF1_PLAOK
    AccessioniPrimary (citable) accession number: P14871
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3