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P14871

- MTF1_PLAOK

UniProt

P14871 - MTF1_PLAOK

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Protein

Modification methylase FokI

Gene

fokIM

Organism
Planomicrobium okeanokoites (Planococcus okeanokoites) (Flavobacterium okeanokoites)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence 5'-GGATG-3' in one strand and 3'-CATCC-5' in the other, causes specific methylation on the A of both strands, and protects the DNA from cleavage by the FokI endonuclease. The 2 domains of the protein participate in modification of the two strands.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3405. M.FokI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase FokI (EC:2.1.1.72)
Short name:
M.FokI
Alternative name(s):
Adenine-specific methyltransferase FokI
Gene namesi
Name:fokIM
Synonyms:mfoKI
OrganismiPlanomicrobium okeanokoites (Planococcus okeanokoites) (Flavobacterium okeanokoites)
Taxonomic identifieri244 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPlanococcaceaePlanomicrobium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 647647Modification methylase FokIPRO_0000087960Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP14871.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 4 hits.
InterProiIPR012186. Ade-mod_methylase_MStsI.
IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 2 hits.
[Graphical view]
PIRSFiPIRSF036638. M_m6A_StsI. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14871-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRFIGSKVNL LDNIQEVIEE NVKDDAHVFM DLFSGTGIVG ENFKKDYQVL
60 70 80 90 100
SNDSLYFSYI LLKAKIENNS IPNFSELKKI GIKEPLHYLE NEEFEISHEF
110 120 130 140 150
FLTHNYSPYM GCERMYFTVE NASRIDFIRL TLNRWKNESL INELEFAYLL
160 170 180 190 200
AILIEAVPFI SNISGTYGAY LKHWDKRALG KLKLRTLDIG NNHYANKTYN
210 220 230 240 250
EDANSLIEKV YGDILYIDPP YNGRQYISNY HLLETIALYD YPEIYGKTGL
260 270 280 290 300
RPYVESKSLY CQKKEVGNAF NHLIEKANFR HILVSYSSEG LLLEEEIESI
310 320 330 340 350
LKSHGLPETY RIYKMPYRKY KSKHKQEASE LHEYIFYIQK DIALTNSVKS
360 370 380 390 400
NKKIEVGKHK TNSYIKSPLN YVGGKHKLLN QIVPLFPDKI DTFVDLFSGG
410 420 430 440 450
FNVGINVNAN KIIATDINTY VVEVLDTMKK TSVEEVIAHI ERRIEEYGLS
460 470 480 490 500
KSNEEGFKAF RNYYNKTKKP LDLYTLICYS FNYQFRFNNN QEYNNPFGRE
510 520 530 540 550
RSQFSPALKK KLVLFIEALH EKNVQFVCSE FEHFNFSQLD QNDLVYCDPP
560 570 580 590 600
YLITTGSYND GNRGFKDWNR LQEIKLLDIL DHLNSKGVYF ALSNVLSHKG
610 620 630 640
LENELLLEWS KKYNIHHLQH SYSNSSHNTT RGESQEVLIT NYTNYTK
Length:647
Mass (Da):75,626
Last modified:April 1, 1990 - v1
Checksum:i66F7D05F17345836
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04623 Genomic DNA. Translation: AAA24926.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04623 Genomic DNA. Translation: AAA24926.1 .

3D structure databases

ProteinModelPortali P14871.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3405. M.FokI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 4 hits.
InterProi IPR012186. Ade-mod_methylase_MStsI.
IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 2 hits.
[Graphical view ]
PIRSFi PIRSF036638. M_m6A_StsI. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 3 hits.
TIGRFAMsi TIGR00571. dam. 1 hit.
PROSITEi PS00092. N6_MTASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The fokI restriction-modification system. I. Organization and nucleotide sequences of the restriction and modification genes."
    Kita K., Kotani H., Sugisaki H., Takanami M.
    J. Biol. Chem. 264:5751-5756(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-9.
    Strain: ATCC 33414 / DSM 15489 / NBRC 12536 / NCIMB 561 / CIP 105082 / LMG 4030 / VKM B-1175.
  2. "The FokI restriction-modification system. II. Presence of two domains in FokI methylase responsible for modification of different DNA strands."
    Sugisaki H., Kita K., Takanami M.
    J. Biol. Chem. 264:5757-5761(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAINS.

Entry informationi

Entry nameiMTF1_PLAOK
AccessioniPrimary (citable) accession number: P14871
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 29, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3