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P14871

- MTF1_PLAOK

UniProt

P14871 - MTF1_PLAOK

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Protein
Modification methylase FokI
Gene
fokIM, mfoKI
Organism
Planomicrobium okeanokoites (Planococcus okeanokoites) (Flavobacterium okeanokoites)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence 5'-GGATG-3' in one strand and 3'-CATCC-5' in the other, causes specific methylation on the A of both strands, and protects the DNA from cleavage by the FokI endonuclease. The 2 domains of the protein participates in modification of the two strands.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3405. M.FokI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase FokI (EC:2.1.1.72)
Short name:
M.FokI
Alternative name(s):
Adenine-specific methyltransferase FokI
Gene namesi
Name:fokIM
Synonyms:mfoKI
OrganismiPlanomicrobium okeanokoites (Planococcus okeanokoites) (Flavobacterium okeanokoites)
Taxonomic identifieri244 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPlanococcaceaePlanomicrobium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 647647Modification methylase FokI
PRO_0000087960Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP14871.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 4 hits.
InterProiIPR012186. Ade-mod_methylase_MStsI.
IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 2 hits.
[Graphical view]
PIRSFiPIRSF036638. M_m6A_StsI. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14871-1 [UniParc]FASTAAdd to Basket

« Hide

MRFIGSKVNL LDNIQEVIEE NVKDDAHVFM DLFSGTGIVG ENFKKDYQVL    50
SNDSLYFSYI LLKAKIENNS IPNFSELKKI GIKEPLHYLE NEEFEISHEF 100
FLTHNYSPYM GCERMYFTVE NASRIDFIRL TLNRWKNESL INELEFAYLL 150
AILIEAVPFI SNISGTYGAY LKHWDKRALG KLKLRTLDIG NNHYANKTYN 200
EDANSLIEKV YGDILYIDPP YNGRQYISNY HLLETIALYD YPEIYGKTGL 250
RPYVESKSLY CQKKEVGNAF NHLIEKANFR HILVSYSSEG LLLEEEIESI 300
LKSHGLPETY RIYKMPYRKY KSKHKQEASE LHEYIFYIQK DIALTNSVKS 350
NKKIEVGKHK TNSYIKSPLN YVGGKHKLLN QIVPLFPDKI DTFVDLFSGG 400
FNVGINVNAN KIIATDINTY VVEVLDTMKK TSVEEVIAHI ERRIEEYGLS 450
KSNEEGFKAF RNYYNKTKKP LDLYTLICYS FNYQFRFNNN QEYNNPFGRE 500
RSQFSPALKK KLVLFIEALH EKNVQFVCSE FEHFNFSQLD QNDLVYCDPP 550
YLITTGSYND GNRGFKDWNR LQEIKLLDIL DHLNSKGVYF ALSNVLSHKG 600
LENELLLEWS KKYNIHHLQH SYSNSSHNTT RGESQEVLIT NYTNYTK 647
Length:647
Mass (Da):75,626
Last modified:April 1, 1990 - v1
Checksum:i66F7D05F17345836
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04623 Genomic DNA. Translation: AAA24926.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04623 Genomic DNA. Translation: AAA24926.1 .

3D structure databases

ProteinModelPortali P14871.
ModBasei Search...

Protein family/group databases

REBASEi 3405. M.FokI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 4 hits.
InterProi IPR012186. Ade-mod_methylase_MStsI.
IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 2 hits.
[Graphical view ]
PIRSFi PIRSF036638. M_m6A_StsI. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 3 hits.
TIGRFAMsi TIGR00571. dam. 1 hit.
PROSITEi PS00092. N6_MTASE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The fokI restriction-modification system. I. Organization and nucleotide sequences of the restriction and modification genes."
    Kita K., Kotani H., Sugisaki H., Takanami M.
    J. Biol. Chem. 264:5751-5756(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-9.
    Strain: ATCC 33414 / DSM 15489 / NBRC 12536 / NCIMB 561 / CIP 105082 / LMG 4030 / VKM B-1175.
  2. "The FokI restriction-modification system. II. Presence of two domains in FokI methylase responsible for modification of different DNA strands."
    Sugisaki H., Kita K., Takanami M.
    J. Biol. Chem. 264:5757-5761(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAINS.

Entry informationi

Entry nameiMTF1_PLAOK
AccessioniPrimary (citable) accession number: P14871
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 11, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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