Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P14870

- T2F1_PLAOK

UniProt

P14870 - T2F1_PLAOK

Protein

Type-2 restriction enzyme FokI

Gene

fokIR

Organism
Planomicrobium okeanokoites (Planococcus okeanokoites) (Flavobacterium okeanokoites)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Recognizes the double-stranded sequences GGATG and CATCC and cleaves respectively 14 bases after G-1 and 13 bases before C-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium

    Protein family/group databases

    REBASEi1056. FokI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme FokI (EC:3.1.21.4)
    Short name:
    R.FokI
    Alternative name(s):
    Endonuclease FokI
    Type II restriction enzyme FokI
    Type IIS restriction enzyme FokI
    Gene namesi
    Name:fokIR
    Synonyms:rfoKI
    OrganismiPlanomicrobium okeanokoites (Planococcus okeanokoites) (Flavobacterium okeanokoites)
    Taxonomic identifieri244 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPlanococcaceaePlanomicrobium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 583583Type-2 restriction enzyme FokIPRO_0000077308Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer. Homodimer when bound to DNA.1 Publication

    Structurei

    Secondary structure

    1
    583
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi21 – 288
    Helixi36 – 438
    Helixi45 – 495
    Helixi53 – 6412
    Beta strandi65 – 673
    Helixi72 – 765
    Helixi83 – 853
    Helixi91 – 955
    Beta strandi102 – 1054
    Helixi108 – 12013
    Beta strandi123 – 1275
    Turni128 – 1314
    Beta strandi132 – 1354
    Helixi137 – 1448
    Helixi150 – 16112
    Helixi164 – 17310
    Helixi181 – 1855
    Beta strandi188 – 1903
    Helixi201 – 2099
    Helixi213 – 22210
    Helixi226 – 24015
    Beta strandi243 – 2464
    Beta strandi249 – 2524
    Beta strandi262 – 27110
    Helixi273 – 28311
    Beta strandi286 – 2883
    Helixi297 – 2993
    Helixi307 – 32317
    Beta strandi325 – 3284
    Helixi329 – 33810
    Helixi345 – 35612
    Turni357 – 3593
    Beta strandi362 – 3654
    Beta strandi368 – 3714
    Turni381 – 3844
    Helixi386 – 3883
    Helixi393 – 40210
    Beta strandi406 – 4083
    Helixi410 – 4145
    Helixi415 – 4184
    Helixi422 – 4243
    Helixi425 – 43814
    Beta strandi443 – 4464
    Beta strandi449 – 4524
    Beta strandi454 – 4585
    Beta strandi460 – 4645
    Beta strandi467 – 4737
    Helixi483 – 49816
    Turni501 – 5033
    Helixi508 – 5114
    Beta strandi519 – 5268
    Helixi532 – 54312
    Beta strandi546 – 5516
    Helixi552 – 56413
    Helixi569 – 5735
    Beta strandi577 – 5804

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FOKX-ray2.80A8-583[»]
    2FOKX-ray2.30A/B5-583[»]
    ProteinModelPortaliP14870.
    SMRiP14870. Positions 8-583.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP14870.

    Family & Domainsi

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    3.40.91.30. 1 hit.
    InterProiIPR011335. Restrct_endonuc-II-like.
    IPR011578. Restrct_endonuc_C/endonuc_I.
    IPR015334. Restrct_endonuc_II_FokI_C.
    IPR004233. Restrct_endonuc_II_FokI_cat.
    IPR004234. Restrct_endonuc_II_FokI_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF09254. Endonuc-FokI_C. 1 hit.
    PF02980. FokI_C. 1 hit.
    PF02981. FokI_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P14870-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFLSMVSKIR TFGWVQNPGK FENLKRVVQV FDRNSKVHNE VKNIKIPTLV    50
    KESKIQKELV AIMNQHDLIY TYKELVGTGT SIRSEAPCDA IIQATIADQG 100
    NKKGYIDNWS SDGFLRWAHA LGFIEYINKS DSFVITDVGL AYSKSADGSA 150
    IEKEILIEAI SSYPPAIRIL TLLEDGQHLT KFDLGKNLGF SGESGFTSLP 200
    EGILLDTLAN AMPKDKGEIR NNWEGSSDKY ARMIGGWLDK LGLVKQGKKE 250
    FIIPTLGKPD NKEFISHAFK ITGEGLKVLR RAKGSTKFTR VPKRVYWEML 300
    ATNLTDKEYV RTRRALILEI LIKAGSLKIE QIQDNLKKLG FDEVIETIEN 350
    DIKGLINTGI FIEIKGRFYQ LKDHILQFVI PNRGVTKQLV KSELEEKKSE 400
    LRHKLKYVPH EYIELIEIAR NSTQDRILEM KVMEFFMKVY GYRGKHLGGS 450
    RKPDGAIYTV GSPIDYGVIV DTKAYSGGYN LPIGQADEMQ RYVEENQTRN 500
    KHINPNEWWK VYPSSVTEFK FLFVSGHFKG NYKAQLTRLN HITNCNGAVL 550
    SVEELLIGGE MIKAGTLTLE EVRRKFNNGE INF 583
    Length:583
    Mass (Da):66,219
    Last modified:April 1, 1990 - v1
    Checksum:iF4AF13DEEFA5CF09
    GO

    Sequence cautioni

    The sequence AAA24934.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04623 Genomic DNA. Translation: AAA24927.1.
    M28828 Genomic DNA. Translation: AAA24934.1. Different initiation.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04623 Genomic DNA. Translation: AAA24927.1 .
    M28828 Genomic DNA. Translation: AAA24934.1 . Different initiation.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FOK X-ray 2.80 A 8-583 [» ]
    2FOK X-ray 2.30 A/B 5-583 [» ]
    ProteinModelPortali P14870.
    SMRi P14870. Positions 8-583.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1056. FokI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P14870.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    3.40.91.30. 1 hit.
    InterProi IPR011335. Restrct_endonuc-II-like.
    IPR011578. Restrct_endonuc_C/endonuc_I.
    IPR015334. Restrct_endonuc_II_FokI_C.
    IPR004233. Restrct_endonuc_II_FokI_cat.
    IPR004234. Restrct_endonuc_II_FokI_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF09254. Endonuc-FokI_C. 1 hit.
    PF02980. FokI_C. 1 hit.
    PF02981. FokI_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The fokI restriction-modification system. I. Organization and nucleotide sequences of the restriction and modification genes."
      Kita K., Kotani H., Sugisaki H., Takanami M.
      J. Biol. Chem. 264:5751-5756(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 33414 / DSM 15489 / NBRC 12536 / NCIMB 561 / CIP 105082 / LMG 4030 / VKM B-1175.
    2. "Nucleotide sequence of the FokI restriction-modification system: separate strand-specificity domains in the methyltransferase."
      Looney M.C., Moran L.S., Jack W.E., Feehery G.R., Benner J.S., Slatko B.E., Wilson G.G.
      Gene 80:193-208(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Structure of the multimodular endonuclease FokI bound to DNA."
      Wah D.A., Hirsch J.A., Dorner L.F., Schildkraut I., Aggarwal A.K.
      Nature 388:97-100(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
      Strain: ATCC 33414 / DSM 15489 / NBRC 12536 / NCIMB 561 / CIP 105082 / LMG 4030 / VKM B-1175.
    4. Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
      Strain: ATCC 33414 / DSM 15489 / NBRC 12536 / NCIMB 561 / CIP 105082 / LMG 4030 / VKM B-1175.
    5. Cited for: SUBUNIT.

    Entry informationi

    Entry nameiT2F1_PLAOK
    AccessioniPrimary (citable) accession number: P14870
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3