Reviewed, UniProtKB/Swiss-Prot P14856 (LOX2_PEA)

Names and origin

Protein names

Recommended name:
    Seed lipoxygenase-2
    EC=1.13.11.12

Gene names

Name:

LOX1.2

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	864 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids, containing a cis,cis-1,4-pentadiene structure.
Catalytic activity	Linoleate + O2 = (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate.
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Lipid metabolism; oxylipin biosynthesis.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis Oxylipin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxylipin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW lipoxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 864	864	Seed lipoxygenase-2		PRO_0000220713
Regions
	Domain	46 – 171	126	PLAT
	Domain	174 – 864	691	Lipoxygenase
Sites
	Metal binding	524	1	Iron; catalytic By similarity
	Metal binding	529	1	Iron; catalytic By similarity
	Metal binding	716	1	Iron; catalytic By similarity
	Metal binding	720	1	Iron; catalytic By similarity
	Metal binding	864	1	Iron; via carboxylate; catalytic By similarity
Experimental info
	Sequence conflict	333	1	L → I in CAA55318. Ref.2
	Sequence conflict	562	1	Missing in CAA55318. Ref.2
	Sequence conflict	578	1	L → I in CAA55318. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P14856-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 2919AF5FAF272CDF	FASTA	864	97,134
	10 20 30 40 50 60 MFPNVTGLLN KGHKIRGTVV LMRKNVLDFN TIVSIGGGNV HGVIDSGINI IGSTLDGLTA 70 80 90 100 110 120 FLGRSVSLQL ISATKSDANG KGKVGKDTFL EGVLASLPTL GAGESAFNIH FEWDHEMGIP 130 140 150 160 170 180 GAFYIKNYMQ VEFFLKSLTL EDVPNHGTIR FVCNSWVYNS KLYKSPRIFF ANKSYLPSET 190 200 210 220 230 240 PSPLVKYREE ELQTLRGDGT GERKLHERIY DYDVYNDLGN PDHGEHLARP ILGGSSTHPY 250 260 270 280 290 300 PRRGRTGRYP TRKDPNSEKP ATETYVPRDE NFGHLKSSDF LAYGIKSVSQ CVVPAFESAF 310 320 330 340 350 360 DLNFTPNEFD SFQDVRNLFE GGIKLPLDVI STLSPLPVVK EIFRTDGEQV LKFTPPHVIR 370 380 390 400 410 420 VSKSAWMTDE EFAREMLAGV NPCMIRGLQE FPPKSNLDPA EYGDHTSKIS VDVLNLDGCT 430 440 450 460 470 480 IDEALASGRL FILDYHDTFI PFLRRINETS AKAYATRTIL FLKENGTLKP VAIELSLPHP 490 500 510 520 530 540 DGDKSGFVSK VILPADEGVE STIWLLAKAY VVVNDSCYHQ LMSHWLNTHA VIEPFVIATN 550 560 570 580 590 600 RQLSVVHPIN KLLAPHYRDT MMNINALARD SLINANGLIE RSFLPSKYAV EMSSAVYKYW 610 620 630 640 650 660 VFTDQALPND LIKRNMAVKD SSSPYGLRLL IEDYPYAVDG LEIWTAIKTW VQDYVSLYYA 670 680 690 700 710 720 TDNDIKNDSE LQHWWKEVVE KGHGDLKDKP WWPKLQTFDE LVEVCTIIIW TASALHAAVN 730 740 750 760 770 780 FGQYPYGGLI LNRPTLSRRL LPEEGTAEYD EMVKSSQKAY LRTITPKFQT LIDLSVIEIL 790 800 810 820 830 840 SRHASDEVYL GQRENPHWTS DSKALQAFQK FGNKLAEIEA KLTNKNNDPS LYHRVGPVQL 850 860 PYTLLHPSSK EGLTFRGIPN SISI

References

[1]	"The cDNA cloning of a pea (Pisum sativum) seed lipoxygenase. Sequence comparisons of the two major pea seed lipoxygenase isoforms." Ealing P.M., Casey R. Biochem. J. 264:929-932(1989) [PubMed: 2515855] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Birte.
[2]	Casey R. Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Birte.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X17061 mRNA. Translation: CAA34906.1. X78580 Genomic DNA. Translation: CAA55318.1.
PIR	S07075.
3D structure databases
HSSP	HSSP built from PDB template 2SBL based on UniProtKB P08170.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.13.11.12. 287.
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001246. LipOase_pln. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00468. PLTLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX2_PEA

Accession

Primary (citable) accession number: P14856

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)