P14848 (LGB2_SESRO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leghemoglobin 2

Alternative name(s):
Srglb2

Gene names

Name:

GLB2

Organism

Sesbania rostrata

Taxonomic identifier

3895 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Sesbanieae › Sesbania

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Provides oxygen to the bacteroids. This role is essential for symbiotic nitrogen fixation.
Subunit structure	Monomer.
Tissue specificity	Root nodules.
Sequence similarities	Belongs to the plant globin family.

Ontologies

Keywords
Biological process	Nitrogen fixation Oxygen transport Transport
Ligand	Heme Iron Metal-binding
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 148	147	Leghemoglobin 2		PRO_0000193000
Sites
	Metal binding	63	1	Iron (heme distal ligand) By similarity
	Metal binding	95	1	Iron (heme proximal ligand) By similarity
Experimental info
	Sequence conflict	5	1	E → D AA sequence Ref.3
	Sequence conflict	20	1	Q → K AA sequence Ref.3
	Sequence conflict	25	1	N → H AA sequence Ref.3
	Sequence conflict	37	1	A → E AA sequence Ref.3
	Sequence conflict	43	1	M → L AA sequence Ref.3
	Sequence conflict	72 – 74	3	RDS → HDA AA sequence Ref.3
	Sequence conflict	101	1	L → T AA sequence Ref.3
	Sequence conflict	130	1	N → I AA sequence Ref.3
	Sequence conflict	140	1	S → A AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P14848 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 949AA649CBE303EE	FASTA	148	15,800
	10 20 30 40 50 60 MGFTEKQEAL VNASYEAFKQ NLPGNSVLFY SFILEKAPAA KGMFSFLKDS DGVPQNNPSL 70 80 90 100 110 120 QAHAEKVFGL VRDSAAQLRA TGVVVLADAS LGSVHVQKGV LDPHFVVVKE ALLKTLKEAA 130 140 GATWSDEVSN AWEVAYDGLS AAIKKAMS

References

[1]	"Primary structure and promoter analysis of leghemoglobin genes of the stem-nodulated tropical legume Sesbania rostrata: conserved coding sequences, cis-elements and trans-acting factors." Metz B.A., Welters P., Hoffmann H.J., Jensen E.O., Schell J., de Bruijn F.J. Mol. Gen. Genet. 214:181-191(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Leaf.
[2]	Welters P., Metz B., Schell J., de Bruijn F.J. Submitted (MAY-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The amino acid sequence of leghemoglobin II from Sesbania rostrata stem nodules." Kortt A.A., Strike P.M., Bogusz D., Appleby C.A. Biochem. Int. 15:509-516(1987) Cited for: PROTEIN SEQUENCE OF 2-148. Tissue: Stem nodule.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13505 Genomic DNA. Translation: CAA31859.1. X13815 Genomic DNA. Translation: CAA32044.1.
PIR	GPFJG2. S08322. S20162.
3D structure databases
ProteinModelPortal	P14848.
SMR	P14848. Positions 1-146.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.490.10. 1 hit.
InterPro	IPR000971. Globin. IPR009050. Globin-like. IPR012292. Globin_dom. IPR001032. Leghaemoglobin. IPR019824. Leghaemoglobin_Fe_BS. [Graphical view]
Pfam	PF00042. Globin. 1 hit. [Graphical view]
PRINTS	PR00188. PLANTGLOBIN.
SUPFAM	SSF46458. Globin_like. 1 hit.
PROSITE	PS01033. GLOBIN. 1 hit. PS00208. PLANT_GLOBIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LGB2_SESRO

Accession

Primary (citable) accession number: P14848

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program