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Reviewed, UniProtKB/Swiss-Prot P14843 (AROF_YEAST)

Last modified November 3, 2009. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited
    EC=2.5.1.54
Alternative name(s):
    Phospho-2-keto-3-deoxyheptonate aldolase
    3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
    DAHP synthetase
Gene names
Name: ARO3
Ordered Locus Names: YDR035W
ORF Names: YD9673.07
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Catalytic activity

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Enzyme regulation

Inhibited by phenyalanine.

Pathway

Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.

Induction

By amino acid starvation.

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium. Ref.4

Sequence similarities

Belongs to the class-I DAHP synthetase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited
PRO_0000140849

Amino acid modifications

Modified residue261Phosphothreonine Ref.5 Ref.6

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Sequences

Sequence LengthMass (Da)Tools
P14843-1 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 1EA0EBE7320707F4

FASTA37041,070
        10         20         30         40         50         60 
MFIKNDHAGD RKRLEDWRIK GYDPLTPPDL LQHEFPISAK GEENIIKARD SVCDILNGKD 

        70         80         90        100        110        120 
DRLVIVIGPC SLHDPKAAYD YADRLAKISE KLSKDLLIIM RAYLEKPRTT VGWKGLINDP 

       130        140        150        160        170        180 
DMNNSFQINK GLRISREMFI KLVEKLPIAG EMLDTISPQF LSDCFSLGAI GARTTESQLH 

       190        200        210        220        230        240 
RELASGLSFP IGFKNGTDGG LQVAIDAMRA AAHEHYFLSV TKPGVTAIVG TEGNKDTFLI 

       250        260        270        280        290        300 
LRGGKNGTNF DKESVQNTKK QLEKAGLTDD SQKRIMIDCS HGNSNKDFKN QPKVAKCIYD 

       310        320        330        340        350        360 
QLTEGENSLC GVMIESNINE GRQDIPKEGG REGLKYGCSV TDACIGWEST EQVLELLAEG 

       370 
VRNRRKALKK

« Hide

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References

« Hide 'large scale' references
[1]"Structure of the ARO3 gene of Saccharomyces cerevisiae."
Paravicini G., Braus G., Huetter R.
Mol. Gen. Genet. 214:165-169(1988) [PubMed: 2906401] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-26, MASS SPECTROMETRY.
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-26, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X13514 Genomic DNA. Translation: CAA31865.1.
Z68196 Genomic DNA. Translation: CAA92374.1.
Z74331 Genomic DNA. Translation: CAA98859.1.
AY557657 Genomic DNA. Translation: AAS55983.1.
PIRS22840.
RefSeqNP_010320.1.

3D structure databases

HSSPHSSP built from PDB template 1HFB based on UniProtKB P32449.
ModBaseSearch...

Protein-protein interaction databases

IntActP14843. 14 interactions.
STRINGP14843.

Proteomic databases

PeptideAtlasP14843.

Genome annotation databases

EnsemblYDR035W; YDR035W; YDR035W; Saccharomyces cerevisiae. [Genome view]
GeneID851605.
GenomeReviewsGene locus YDR035W in contig Z71256_GR.
KEGGsce:YDR035W.
NMPDRfig|4932.3.peg.1065.

Organism-specific databases

CYGDYDR035w.
SGDS000002442. ARO3.

Phylogenomic databases

HOGENOMP14843.
OMATEDWRIR.

Enzyme and pathway databases

BRENDA2.5.1.54. 250.

Gene expression databases

ArrayExpressP14843.
GenevestigatorP14843.
GermOnlineYDR035W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006219. DHAP_synth_1.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHERPTHR21225. AroFGH. 1 hit.
PfamPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
PIRSFPIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMsTIGR00034. aroFGH. 1 hit.
ProtoNetSearch...

Other Resources

NextBio969109.

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Entry information

Entry nameAROF_YEAST
AccessionPrimary (citable) accession number: P14843
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 3, 2009
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents