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Protein

Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited

Gene

ARO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Catalytic activityi

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Enzyme regulationi

Inhibited by phenyalanine.

Pathwayi: chorismate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited (ARO4), Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited (ARO3)
  2. Pentafunctional AROM polypeptide (ARO1)
  3. Pentafunctional AROM polypeptide (ARO1)
  4. Pentafunctional AROM polypeptide (ARO1)
  5. Pentafunctional AROM polypeptide (ARO1)
  6. Pentafunctional AROM polypeptide (ARO1)
  7. Chorismate synthase (ARO2)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

  • 3-deoxy-7-phosphoheptulonate synthase activity Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Stress response

Enzyme and pathway databases

BioCyciYEAST:YDR035W-MONOMER.
UniPathwayiUPA00053; UER00084.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited (EC:2.5.1.54)
Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase
Gene namesi
Name:ARO3
Ordered Locus Names:YDR035W
ORF Names:YD9673.07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR035W.
SGDiS000002442. ARO3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001408491 – 370Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibitedAdd BLAST370

Proteomic databases

MaxQBiP14843.
PRIDEiP14843.
TopDownProteomicsiP14843.

PTM databases

iPTMnetiP14843.

Expressioni

Inductioni

By amino acid starvation.

Interactioni

Protein-protein interaction databases

BioGridi32090. 17 interactors.
IntActiP14843. 6 interactors.
MINTiMINT-4480402.

Structurei

3D structure databases

ProteinModelPortaliP14843.
SMRiP14843.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-I DAHP synthase family.Curated

Phylogenomic databases

HOGENOMiHOG000220501.
InParanoidiP14843.
KOiK01626.
OMAiAHEHYFL.
OrthoDBiEOG092C2RSR.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006219. DHAP_synth_1.
[Graphical view]
PANTHERiPTHR21225. PTHR21225. 1 hit.
PfamiPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMsiTIGR00034. aroFGH. 1 hit.

Sequencei

Sequence statusi: Complete.

P14843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFIKNDHAGD RKRLEDWRIK GYDPLTPPDL LQHEFPISAK GEENIIKARD
60 70 80 90 100
SVCDILNGKD DRLVIVIGPC SLHDPKAAYD YADRLAKISE KLSKDLLIIM
110 120 130 140 150
RAYLEKPRTT VGWKGLINDP DMNNSFQINK GLRISREMFI KLVEKLPIAG
160 170 180 190 200
EMLDTISPQF LSDCFSLGAI GARTTESQLH RELASGLSFP IGFKNGTDGG
210 220 230 240 250
LQVAIDAMRA AAHEHYFLSV TKPGVTAIVG TEGNKDTFLI LRGGKNGTNF
260 270 280 290 300
DKESVQNTKK QLEKAGLTDD SQKRIMIDCS HGNSNKDFKN QPKVAKCIYD
310 320 330 340 350
QLTEGENSLC GVMIESNINE GRQDIPKEGG REGLKYGCSV TDACIGWEST
360 370
EQVLELLAEG VRNRRKALKK
Length:370
Mass (Da):41,070
Last modified:April 1, 1990 - v1
Checksum:i1EA0EBE7320707F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13514 Genomic DNA. Translation: CAA31865.1.
Z68196 Genomic DNA. Translation: CAA92374.1.
Z74331 Genomic DNA. Translation: CAA98859.1.
AY557657 Genomic DNA. Translation: AAS55983.1.
BK006938 Genomic DNA. Translation: DAA11883.1.
PIRiS22840.
RefSeqiNP_010320.3. NM_001180343.3.

Genome annotation databases

EnsemblFungiiYDR035W; YDR035W; YDR035W.
GeneIDi851605.
KEGGisce:YDR035W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13514 Genomic DNA. Translation: CAA31865.1.
Z68196 Genomic DNA. Translation: CAA92374.1.
Z74331 Genomic DNA. Translation: CAA98859.1.
AY557657 Genomic DNA. Translation: AAS55983.1.
BK006938 Genomic DNA. Translation: DAA11883.1.
PIRiS22840.
RefSeqiNP_010320.3. NM_001180343.3.

3D structure databases

ProteinModelPortaliP14843.
SMRiP14843.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32090. 17 interactors.
IntActiP14843. 6 interactors.
MINTiMINT-4480402.

PTM databases

iPTMnetiP14843.

Proteomic databases

MaxQBiP14843.
PRIDEiP14843.
TopDownProteomicsiP14843.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR035W; YDR035W; YDR035W.
GeneIDi851605.
KEGGisce:YDR035W.

Organism-specific databases

EuPathDBiFungiDB:YDR035W.
SGDiS000002442. ARO3.

Phylogenomic databases

HOGENOMiHOG000220501.
InParanoidiP14843.
KOiK01626.
OMAiAHEHYFL.
OrthoDBiEOG092C2RSR.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00084.
BioCyciYEAST:YDR035W-MONOMER.

Miscellaneous databases

PROiP14843.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006219. DHAP_synth_1.
[Graphical view]
PANTHERiPTHR21225. PTHR21225. 1 hit.
PfamiPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMsiTIGR00034. aroFGH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROF_YEAST
AccessioniPrimary (citable) accession number: P14843
Secondary accession number(s): D6VS23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 30, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.