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P14843 (AROF_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited
EC=2.5.1.54
Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase
Gene names
Name:ARO3
Ordered Locus Names:YDR035W
ORF Names:YD9673.07
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Catalytic activity

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Enzyme regulation

Inhibited by phenyalanine.

Pathway

Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.

Induction

By amino acid starvation.

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the class-I DAHP synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited
PRO_0000140849

Amino acid modifications

Modified residue261Phosphothreonine Ref.6 Ref.7

Sequences

Sequence LengthMass (Da)Tools
P14843 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 1EA0EBE7320707F4

FASTA37041,070
        10         20         30         40         50         60 
MFIKNDHAGD RKRLEDWRIK GYDPLTPPDL LQHEFPISAK GEENIIKARD SVCDILNGKD 

        70         80         90        100        110        120 
DRLVIVIGPC SLHDPKAAYD YADRLAKISE KLSKDLLIIM RAYLEKPRTT VGWKGLINDP 

       130        140        150        160        170        180 
DMNNSFQINK GLRISREMFI KLVEKLPIAG EMLDTISPQF LSDCFSLGAI GARTTESQLH 

       190        200        210        220        230        240 
RELASGLSFP IGFKNGTDGG LQVAIDAMRA AAHEHYFLSV TKPGVTAIVG TEGNKDTFLI 

       250        260        270        280        290        300 
LRGGKNGTNF DKESVQNTKK QLEKAGLTDD SQKRIMIDCS HGNSNKDFKN QPKVAKCIYD 

       310        320        330        340        350        360 
QLTEGENSLC GVMIESNINE GRQDIPKEGG REGLKYGCSV TDACIGWEST EQVLELLAEG 

       370 
VRNRRKALKK

« Hide

References

« Hide 'large scale' references
[1]"Structure of the ARO3 gene of Saccharomyces cerevisiae."
Paravicini G., Braus G., Huetter R.
Mol. Gen. Genet. 214:165-169(1988) [PubMed: 2906401] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-26, MASS SPECTROMETRY.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-26, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X13514 Genomic DNA. Translation: CAA31865.1.
Z68196 Genomic DNA. Translation: CAA92374.1.
Z74331 Genomic DNA. Translation: CAA98859.1.
AY557657 Genomic DNA. Translation: AAS55983.1.
BK006938 Genomic DNA. Translation: DAA11883.1.
PIRS22840.
RefSeqNP_010320.1. NM_001180343.1.

3D structure databases

ProteinModelPortalP14843.
SMRP14843. Positions 18-370.
ModBaseSearch...

Protein-protein interaction databases

IntActP14843. 6 interactions.
STRINGP14843.

Proteomic databases

PeptideAtlasP14843.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR035W; YDR035W; YDR035W.
GeneID851605.
KEGGsce:YDR035W.
NMPDRfig|4932.3.peg.1065.

Organism-specific databases

CYGDYDR035w.
SGDS000002442. ARO3.

Phylogenomic databases

eggNOGfuNOG04423.
GeneTreeEFGT00050000003783.
HOGENOMHBG670536.
OMAGTEGNED.
OrthoDBEOG4NZZ2W.

Gene expression databases

ArrayExpressP14843.
GenevestigatorP14843.
GermOnlineYDR035W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR013785. Aldolase_TIM.
IPR006218. DAHP1/KDSA.
IPR006219. DHAP_synth_1.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01626.
PANTHERPTHR21225. AroFGH. 1 hit.
PfamPF00793. DAHP_synth_1. 1 hit.
[Graphical view]
PIRSFPIRSF001361. DAHP_synthase. 1 hit.
TIGRFAMsTIGR00034. AroFGH. 1 hit.
ProtoNetSearch...

Other

NextBio969109.

Entry information

Entry nameAROF_YEAST
AccessionPrimary (citable) accession number: P14843
Secondary accession number(s): D6VS23
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: December 14, 2011
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents