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Protein

Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited

Gene

ARO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.

Enzyme regulationi

Inhibited by phenyalanine.

Pathwayi: chorismate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited (ARO4), Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited (ARO3)
  2. Pentafunctional AROM polypeptide (ARO1)
  3. Pentafunctional AROM polypeptide (ARO1)
  4. Pentafunctional AROM polypeptide (ARO1)
  5. Pentafunctional AROM polypeptide (ARO1)
  6. Pentafunctional AROM polypeptide (ARO1)
  7. Chorismate synthase (ARO2)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

  • 3-deoxy-7-phosphoheptulonate synthase activity Source: SGD

GO - Biological processi

  • aromatic amino acid family biosynthetic process Source: GO_Central
  • chorismate biosynthetic process Source: SGD

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Stress response

Enzyme and pathway databases

BioCyciYEAST:YDR035W-MONOMER
UniPathwayiUPA00053; UER00084

Names & Taxonomyi

Protein namesi
Recommended name:
Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited (EC:2.5.1.54)
Alternative name(s):
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
DAHP synthase
Phospho-2-keto-3-deoxyheptonate aldolase
Gene namesi
Name:ARO3
Ordered Locus Names:YDR035W
ORF Names:YD9673.07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR035W
SGDiS000002442 ARO3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001408491 – 370Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibitedAdd BLAST370

Proteomic databases

MaxQBiP14843
PaxDbiP14843
PRIDEiP14843
TopDownProteomicsiP14843

PTM databases

iPTMnetiP14843

Expressioni

Inductioni

By amino acid starvation.

Interactioni

Protein-protein interaction databases

BioGridi32090, 42 interactors
IntActiP14843, 6 interactors
STRINGi4932.YDR035W

Structurei

3D structure databases

ProteinModelPortaliP14843
SMRiP14843
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-I DAHP synthase family.Curated

Phylogenomic databases

GeneTreeiENSGT00910000148669
HOGENOMiHOG000220501
InParanoidiP14843
KOiK01626
OMAiCDILNGK
OrthoDBiEOG092C2RSR

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006218 DAHP1/KDSA
IPR006219 DHAP_synth_1
PANTHERiPTHR21225 PTHR21225, 1 hit
PfamiView protein in Pfam
PF00793 DAHP_synth_1, 1 hit
PIRSFiPIRSF001361 DAHP_synthase, 1 hit
TIGRFAMsiTIGR00034 aroFGH, 1 hit

Sequencei

Sequence statusi: Complete.

P14843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFIKNDHAGD RKRLEDWRIK GYDPLTPPDL LQHEFPISAK GEENIIKARD
60 70 80 90 100
SVCDILNGKD DRLVIVIGPC SLHDPKAAYD YADRLAKISE KLSKDLLIIM
110 120 130 140 150
RAYLEKPRTT VGWKGLINDP DMNNSFQINK GLRISREMFI KLVEKLPIAG
160 170 180 190 200
EMLDTISPQF LSDCFSLGAI GARTTESQLH RELASGLSFP IGFKNGTDGG
210 220 230 240 250
LQVAIDAMRA AAHEHYFLSV TKPGVTAIVG TEGNKDTFLI LRGGKNGTNF
260 270 280 290 300
DKESVQNTKK QLEKAGLTDD SQKRIMIDCS HGNSNKDFKN QPKVAKCIYD
310 320 330 340 350
QLTEGENSLC GVMIESNINE GRQDIPKEGG REGLKYGCSV TDACIGWEST
360 370
EQVLELLAEG VRNRRKALKK
Length:370
Mass (Da):41,070
Last modified:April 1, 1990 - v1
Checksum:i1EA0EBE7320707F4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13514 Genomic DNA Translation: CAA31865.1
Z68196 Genomic DNA Translation: CAA92374.1
Z74331 Genomic DNA Translation: CAA98859.1
AY557657 Genomic DNA Translation: AAS55983.1
BK006938 Genomic DNA Translation: DAA11883.1
PIRiS22840
RefSeqiNP_010320.3, NM_001180343.3

Genome annotation databases

EnsemblFungiiYDR035W; YDR035W; YDR035W
GeneIDi851605
KEGGisce:YDR035W

Entry informationi

Entry nameiAROF_YEAST
AccessioniPrimary (citable) accession number: P14843
Secondary accession number(s): D6VS23
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: May 23, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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