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P14842 (5HT2A_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-hydroxytryptamine receptor 2A
Short name=5-HT-2
Short name=5-HT-2A
Alternative name(s):
Serotonin receptor 2A
Gene names
Name:Htr2a
Synonyms:Htr2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length471 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production.

Subunit structure

Interacts with MPDZ and INADL. May interact with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Note: Localizes to the postsynaptic thickening of axo-dendritic synapses By similarity.

Domain

The PDZ domain-binding motif is involved in the interaction with INADL, CASK, APBA1, DLG1 and DLG4 By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Sequence caution

The sequence CAA32150.1 differs from that shown. Reason: Frameshift at position 37.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processERK1 and ERK2 cascade

Inferred from electronic annotation. Source: Compara

aging

Inferred from expression pattern PubMed 17374516. Source: RGD

cell death

Inferred from mutant phenotype PubMed 16504407. Source: RGD

memory

Inferred from mutant phenotype PubMed 16149040. Source: RGD

negative regulation of potassium ion transport

Inferred from mutant phenotype PubMed 16527989. Source: RGD

negative regulation of synaptic transmission, glutamatergic

Inferred from mutant phenotype PubMed 17016851. Source: RGD

phosphatidylinositol 3-kinase cascade

Inferred from electronic annotation. Source: Compara

phosphatidylinositol biosynthetic process

Inferred from electronic annotation. Source: Compara

phospholipase C-activating serotonin receptor signaling pathway

Inferred from direct assay PubMed 17010161. Source: RGD

positive regulation of MAP kinase activity

Inferred from mutant phenotype PubMed 15087293. Source: RGD

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 15087293. Source: RGD

positive regulation of vasoconstriction

Inferred from mutant phenotype PubMed 16527989. Source: RGD

regulation of dopamine secretion

Inferred from mutant phenotype PubMed 15999145. Source: RGD

release of sequestered calcium ion into cytosol

Inferred from electronic annotation. Source: Compara

response to drug

Inferred from mutant phenotype PubMed 17077317. Source: RGD

response to stress

Inferred from mutant phenotype PubMed 15999145. Source: RGD

sensory perception of pain

Inferred from mutant phenotype PubMed 16527395. Source: RGD

sleep

Inferred from mutant phenotype PubMed 17059817. Source: RGD

temperature homeostasis

Inferred from mutant phenotype PubMed 17067560. Source: RGD

   Cellular_componentcytosol

Inferred from direct assay PubMed 14534355. Source: RGD

dendritic shaft

Inferred from direct assay PubMed 18622042. Source: RGD

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from direct assay PubMed 14534355. Source: RGD

   Molecular_function1-(4-iodo-2,5-dimethoxyphenyl)propan-2-amine binding

Inferred from electronic annotation. Source: Compara

G-protein alpha-subunit binding

Inferred from mutant phenotype PubMed 19016481. Source: RGD

drug binding

Inferred from electronic annotation. Source: Compara

phosphatidylinositol phospholipase C activity

Inferred from electronic annotation. Source: Compara

serotonin binding

Inferred from direct assay PubMed 19016481. Source: RGD

serotonin receptor activity

Inferred from direct assay PubMed 19016481. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4714715-hydroxytryptamine receptor 2A
PRO_0000068951

Regions

Topological domain1 – 7575Extracellular By similarity
Transmembrane76 – 9924Helical; Name=1; By similarity
Topological domain100 – 11011Cytoplasmic By similarity
Transmembrane111 – 13222Helical; Name=2; By similarity
Topological domain133 – 14715Extracellular By similarity
Transmembrane148 – 17124Helical; Name=3; By similarity
Topological domain172 – 19120Cytoplasmic By similarity
Transmembrane192 – 21524Helical; Name=4; By similarity
Topological domain216 – 23318Extracellular By similarity
Transmembrane234 – 25421Helical; Name=5; By similarity
Topological domain255 – 32470Cytoplasmic By similarity
Transmembrane325 – 34622Helical; Name=6; By similarity
Topological domain347 – 36216Extracellular By similarity
Transmembrane363 – 38422Helical; Name=7; By similarity
Topological domain385 – 47187Cytoplasmic By similarity
Motif469 – 4713PDZ-binding

Amino acid modifications

Glycosylation81N-linked (GlcNAc...) Potential
Glycosylation381N-linked (GlcNAc...) Potential
Glycosylation441N-linked (GlcNAc...) Potential
Glycosylation511N-linked (GlcNAc...) Potential
Glycosylation541N-linked (GlcNAc...) Potential
Disulfide bond148 ↔ 227 By similarity

Sequences

Sequence LengthMass (Da)Tools
P14842 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 3626DACE5F6C7FF4

FASTA47152,850
        10         20         30         40         50         60 
MEILCEDNIS LSSIPNSLMQ LGDGPRLYHN DFNSRDANTS EASNWTIDAE NRTNLSCEGY 

        70         80         90        100        110        120 
LPPTCLSILH LQEKNWSALL TTVVIILTIA GNILVIMAVS LEKKLQNATN YFLMSLAIAD 

       130        140        150        160        170        180 
MLLGFLVMPV SMLTILYGYR WPLPSKLCAI WIYLDVLFST ASIMHLCAIS LDRYVAIQNP 

       190        200        210        220        230        240 
IHHSRFNSRT KAFLKIIAVW TISVGISMPI PVFGLQDDSK VFKEGSCLLA DDNFVLIGSF 

       250        260        270        280        290        300 
VAFFIPLTIM VITYFLTIKS LQKEATLCVS DLSTRAKLAS FSFLPQSSLS SEKLFQRSIH 

       310        320        330        340        350        360 
REPGSYAGRR TMQSISNEQK ACKVLGIVFF LFVVMWCPFF ITNIMAVICK ESCNENVIGA 

       370        380        390        400        410        420 
LLNVFVWIGY LSSAVNPLVY TLFNKTYRSA FSRYIQCQYK ENRKPLQLIL VNTIPALAYK 

       430        440        450        460        470 
SSQLQVGQKK NSQEDAEQTV DDCSMVTLGK QQSEENCTDN IETVNEKVSC V

« Hide

References

[1]"The 5HT2 receptor defines a family of structurally distinct but functionally conserved serotonin receptors."
Julius D., Huang K.N., Livelli T.J., Axel R., Jessell T.M.
Proc. Natl. Acad. Sci. U.S.A. 87:928-932(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structure and functional expression of cloned rat serotonin 5HT-2 receptor."
Pritchett D.B., Bach A.W.J., Wozny M., Taleb O., Dal-Toso R., Shih J.C., Seeburg P.H.
EMBO J. 7:4135-4140(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The 5-HT2 serotonin receptor gene Htr-2 is tightly linked to Es-10 on mouse chromosome 14."
Liu J., Chen Y., Kozak C.A., Yu L.
Genomics 11:231-234(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M30705 mRNA. Translation: AAA42178.1.
X13971 mRNA. Translation: CAA32150.1. Frameshift.
M64867 mRNA. No translation available.
IPIIPI00209445.
PIRA34863.
S02011.
RefSeqNP_058950.1. NM_017254.1.
UniGeneRn.10294.
Rn.146756.

3D structure databases

ProteinModelPortalP14842.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000013408.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP14842.

Proteomic databases

PaxDbP14842.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000013408; ENSRNOP00000013408; ENSRNOG00000010063.
GeneID29595.
KEGGrno:29595.
UCSCRGD:61800. rat.

Organism-specific databases

CTD3356.
RGD61800. Htr2a.

Phylogenomic databases

eggNOGNOG247243.
GeneTreeENSGT00700000104081.
HOGENOMHOG000240378.
HOVERGENHBG107487.
InParanoidP14842.
KOK04157.
OMAFITNVMA.
OrthoDBEOG4TMR24.

Gene expression databases

GenevestigatorP14842.
GermOnlineENSRNOG00000010063. Rattus norvegicus.

Family and domain databases

InterProIPR000455. 5HT2A_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00516. 5HT2ARECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP14842.
ChEMBLCHEMBL322.
NextBio609738.

Entry information

Entry name5HT2A_RAT
AccessionPrimary (citable) accession number: P14842
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 3, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

SIMILARITY comments

Index of protein domains and families

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