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Protein

Cystatin-C

Gene

Cst3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei31Reactive site1

GO - Molecular functioni

  • beta-amyloid binding Source: Ensembl
  • cysteine-type endopeptidase inhibitor activity Source: RGD
  • identical protein binding Source: Ensembl
  • peptidase inhibitor activity Source: RGD
  • protease binding Source: RGD

GO - Biological processi

  • apoptotic process Source: RGD
  • brain development Source: RGD
  • cell activation Source: RGD
  • cellular response to hydrogen peroxide Source: RGD
  • cellular response to oxidative stress Source: RGD
  • circadian sleep/wake cycle, REM sleep Source: RGD
  • defense response Source: Ensembl
  • embryo implantation Source: RGD
  • eye development Source: RGD
  • male gonad development Source: RGD
  • negative regulation of blood vessel remodeling Source: Ensembl
  • negative regulation of cell death Source: RGD
  • negative regulation of collagen catabolic process Source: Ensembl
  • negative regulation of cysteine-type endopeptidase activity Source: GO_Central
  • negative regulation of elastin catabolic process Source: Ensembl
  • negative regulation of extracellular matrix disassembly Source: Ensembl
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of DNA replication Source: RGD
  • regulation of programmed cell death Source: RGD
  • response to axon injury Source: RGD
  • response to carbohydrate Source: RGD
  • response to drug Source: RGD
  • response to estradiol Source: RGD
  • response to hypoxia Source: RGD
  • response to inorganic substance Source: RGD
  • response to nutrient levels Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to oxidative stress Source: RGD
  • response to toxic substance Source: RGD
  • salivary gland development Source: RGD
  • Sertoli cell development Source: RGD
  • supramolecular fiber organization Source: Ensembl

Keywordsi

Molecular functionProtease inhibitor, Thiol protease inhibitor

Protein family/group databases

MEROPSiI25.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystatin-C
Alternative name(s):
Cystatin-3
Gene namesi
Name:Cst3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi2432. Cst3.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • basement membrane Source: RGD
  • cell projection Source: RGD
  • contractile fiber Source: RGD
  • cytoplasm Source: RGD
  • endoplasmic reticulum Source: RGD
  • extracellular exosome Source: Ensembl
  • extracellular space Source: RGD
  • lysosome Source: RGD
  • multivesicular body Source: RGD
  • neuronal cell body Source: RGD
  • nuclear membrane Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • vesicle Source: RGD

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000000664421 – 140Cystatin-CAdd BLAST120

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi93 ↔ 103By similarity
Disulfide bondi117 ↔ 137By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP14841.
PRIDEiP14841.

PTM databases

iPTMnetiP14841.
PhosphoSitePlusiP14841.
UniCarbKBiP14841.

Expressioni

Gene expression databases

BgeeiENSRNOG00000005195.
GenevisibleiP14841. RN.

Interactioni

GO - Molecular functioni

  • identical protein binding Source: Ensembl
  • protease binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007175.

Structurei

3D structure databases

ProteinModelPortaliP14841.
SMRiP14841.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi75 – 79Secondary area of contact5

Sequence similaritiesi

Belongs to the cystatin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IZZH. Eukaryota.
ENOG4112CFJ. LUCA.
GeneTreeiENSGT00880000137924.
HOGENOMiHOG000231754.
HOVERGENiHBG009556.
InParanoidiP14841.
KOiK13899.
OMAiYTVPWLG.
OrthoDBiEOG091G0TP1.
PhylomeDBiP14841.

Family and domain databases

CDDicd00042. CY. 1 hit.
InterProiView protein in InterPro
IPR027214. Cystatin.
IPR000010. Cystatin_dom.
IPR018073. Prot_inh_cystat_CS.
PANTHERiPTHR11413. PTHR11413. 1 hit.
PfamiView protein in Pfam
PF00031. Cystatin. 1 hit.
SMARTiView protein in SMART
SM00043. CY. 1 hit.
PROSITEiView protein in PROSITE
PS00287. CYSTATIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPLRSLML LLAVLAVAWA GTSRPPPRLL GAPQEADASE EGVQRALDFA
60 70 80 90 100
VSEYNKGSND AYHSRAIQVV RARKQLVAGI NYYLDVEMGR TTCTKSQTNL
110 120 130 140
TNCPFHDQPH LMRKALCSFQ IYSVPWKGTH TLTKSSCKNA
Length:140
Mass (Da):15,437
Last modified:December 6, 2005 - v2
Checksum:iB785B2958F7824F8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti38A → E AA sequence (PubMed:2400577).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087591 mRNA. Translation: AAH87591.1.
X16957 mRNA. Translation: CAA34831.1.
PIRiS07085.
S10587.
RefSeqiNP_036969.1. NM_012837.1.
UniGeneiRn.106351.

Genome annotation databases

EnsembliENSRNOT00000007175; ENSRNOP00000007175; ENSRNOG00000005195.
GeneIDi25307.
KEGGirno:25307.
UCSCiRGD:2432. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCYTC_RAT
AccessioniPrimary (citable) accession number: P14841
Secondary accession number(s): Q5M968
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: December 6, 2005
Last modified: July 5, 2017
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families