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Protein

Cystatin-C

Gene

Cst3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei31 – 311Reactive site

GO - Molecular functioni

  • cysteine-type endopeptidase inhibitor activity Source: RGD
  • peptidase inhibitor activity Source: RGD
  • protease binding Source: RGD

GO - Biological processi

  • apoptotic process Source: RGD
  • brain development Source: RGD
  • cell activation Source: RGD
  • cellular response to hydrogen peroxide Source: RGD
  • cellular response to oxidative stress Source: RGD
  • circadian sleep/wake cycle, REM sleep Source: RGD
  • embryo implantation Source: RGD
  • eye development Source: RGD
  • male gonad development Source: RGD
  • negative regulation of cell death Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of DNA replication Source: RGD
  • regulation of programmed cell death Source: RGD
  • response to axon injury Source: RGD
  • response to carbohydrate Source: RGD
  • response to drug Source: RGD
  • response to estradiol Source: RGD
  • response to hypoxia Source: RGD
  • response to inorganic substance Source: RGD
  • response to nutrient levels Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to oxidative stress Source: RGD
  • response to toxic substance Source: RGD
  • salivary gland development Source: RGD
  • Sertoli cell development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Thiol protease inhibitor

Protein family/group databases

MEROPSiI25.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystatin-C
Alternative name(s):
Cystatin-3
Gene namesi
Name:Cst3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi2432. Cst3.

Subcellular locationi

GO - Cellular componenti

  • axon Source: RGD
  • basement membrane Source: RGD
  • cell projection Source: RGD
  • contractile fiber Source: RGD
  • cytoplasm Source: RGD
  • endoplasmic reticulum Source: RGD
  • extracellular space Source: RGD
  • lysosome Source: RGD
  • multivesicular body Source: RGD
  • neuronal cell body Source: RGD
  • nuclear membrane Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 140120Cystatin-CPRO_0000006644Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi93 ↔ 103By similarity
Disulfide bondi117 ↔ 137By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP14841.
PRIDEiP14841.

PTM databases

iPTMnetiP14841.
PhosphoSiteiP14841.
UniCarbKBiP14841.

Expressioni

Gene expression databases

BgeeiENSRNOG00000005195.
GenevisibleiP14841. RN.

Interactioni

GO - Molecular functioni

  • protease binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007175.

Structurei

3D structure databases

ProteinModelPortaliP14841.
SMRiP14841. Positions 27-140.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi75 – 795Secondary area of contact

Sequence similaritiesi

Belongs to the cystatin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IZZH. Eukaryota.
ENOG4112CFJ. LUCA.
GeneTreeiENSGT00840000129787.
HOGENOMiHOG000231754.
HOVERGENiHBG009556.
InParanoidiP14841.
KOiK13899.
OMAiYTVPWLG.
OrthoDBiEOG091G0TP1.
PhylomeDBiP14841.

Family and domain databases

InterProiIPR027214. Cystatin.
IPR000010. Cystatin_dom.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 1 hit.
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 1 hit.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPLRSLML LLAVLAVAWA GTSRPPPRLL GAPQEADASE EGVQRALDFA
60 70 80 90 100
VSEYNKGSND AYHSRAIQVV RARKQLVAGI NYYLDVEMGR TTCTKSQTNL
110 120 130 140
TNCPFHDQPH LMRKALCSFQ IYSVPWKGTH TLTKSSCKNA
Length:140
Mass (Da):15,437
Last modified:December 6, 2005 - v2
Checksum:iB785B2958F7824F8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 381A → E AA sequence (PubMed:2400577).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087591 mRNA. Translation: AAH87591.1.
X16957 mRNA. Translation: CAA34831.1.
PIRiS07085.
S10587.
RefSeqiNP_036969.1. NM_012837.1.
UniGeneiRn.106351.

Genome annotation databases

EnsembliENSRNOT00000007175; ENSRNOP00000007175; ENSRNOG00000005195.
GeneIDi25307.
KEGGirno:25307.
UCSCiRGD:2432. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC087591 mRNA. Translation: AAH87591.1.
X16957 mRNA. Translation: CAA34831.1.
PIRiS07085.
S10587.
RefSeqiNP_036969.1. NM_012837.1.
UniGeneiRn.106351.

3D structure databases

ProteinModelPortaliP14841.
SMRiP14841. Positions 27-140.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007175.

Protein family/group databases

MEROPSiI25.004.

PTM databases

iPTMnetiP14841.
PhosphoSiteiP14841.
UniCarbKBiP14841.

Proteomic databases

PaxDbiP14841.
PRIDEiP14841.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007175; ENSRNOP00000007175; ENSRNOG00000005195.
GeneIDi25307.
KEGGirno:25307.
UCSCiRGD:2432. rat.

Organism-specific databases

CTDi1471.
RGDi2432. Cst3.

Phylogenomic databases

eggNOGiENOG410IZZH. Eukaryota.
ENOG4112CFJ. LUCA.
GeneTreeiENSGT00840000129787.
HOGENOMiHOG000231754.
HOVERGENiHBG009556.
InParanoidiP14841.
KOiK13899.
OMAiYTVPWLG.
OrthoDBiEOG091G0TP1.
PhylomeDBiP14841.

Miscellaneous databases

PROiP14841.

Gene expression databases

BgeeiENSRNOG00000005195.
GenevisibleiP14841. RN.

Family and domain databases

InterProiIPR027214. Cystatin.
IPR000010. Cystatin_dom.
IPR018073. Prot_inh_cystat_CS.
[Graphical view]
PANTHERiPTHR11413. PTHR11413. 1 hit.
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 1 hit.
[Graphical view]
PROSITEiPS00287. CYSTATIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYTC_RAT
AccessioniPrimary (citable) accession number: P14841
Secondary accession number(s): Q5M968
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: December 6, 2005
Last modified: September 7, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.