P14841 (CYTC_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cystatin-C

Alternative name(s):
Cystatin-3

Gene names

Name:

Cst3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Known to inhibit cathepsin B, H, and L.
Subcellular location	Secreted.
Sequence similarities	Belongs to the cystatin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Protease inhibitor Thiol protease inhibitor
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Sertoli cell development Inferred from expression pattern PubMed 9432134. Source: RGD apoptotic process Inferred from expression pattern PubMed 16414118. Source: RGD brain development Inferred from expression pattern PubMed 2471663. Source: RGD cell activation Inferred from expression pattern PubMed 16521186. Source: RGD cellular response to hydrogen peroxide Inferred from expression pattern PubMed 17440810. Source: RGD circadian sleep/wake cycle, REM sleep Inferred from expression pattern PubMed 17027151. Source: RGD defense response Inferred from electronic annotation. Source: Compara embryo implantation Inferred from expression pattern PubMed 16935259. Source: RGD extracellular fibril organization Inferred from electronic annotation. Source: Compara eye development Inferred from expression pattern PubMed 11431459. Source: RGD negative regulation of blood vessel remodeling Inferred from electronic annotation. Source: Compara negative regulation of cell death Inferred from mutant phenotype PubMed 16140167. Source: RGD negative regulation of collagen catabolic process Inferred from electronic annotation. Source: Compara negative regulation of elastin catabolic process Inferred from electronic annotation. Source: Compara negative regulation of extracellular matrix disassembly Inferred from electronic annotation. Source: Compara positive regulation of DNA replication Inferred from direct assay PubMed 1540157. Source: RGD positive regulation of cell proliferation Inferred from direct assay PubMed 11144350PubMed 1540157. Source: RGD regulation of programmed cell death Inferred from mutant phenotype PubMed 16140167. Source: RGD response to axon injury Inferred from expression pattern PubMed 12044447PubMed 14745560PubMed 8738161PubMed 9147287. Source: RGD response to carbohydrate stimulus Inferred from expression pattern PubMed 11246163PubMed 9222450. Source: RGD response to drug Inferred from expression pattern PubMed 16248890. Source: RGD response to estradiol stimulus Inferred from expression pattern PubMed 16935259. Source: RGD response to hypoxia Inferred from expression pattern PubMed 8590041. Source: RGD response to nutrient levels Inferred from expression pattern PubMed 18723004. Source: RGD response to toxin Inferred from expression pattern PubMed 16414118. Source: RGD salivary gland development Inferred from expression pattern PubMed 10075146. Source: RGD
Cellular_component	axon part Inferred from direct assay PubMed 14738488. Source: RGD basement membrane Inferred from direct assay PubMed 15872057. Source: RGD contractile fiber Inferred from direct assay PubMed 8245434. Source: RGD endoplasmic reticulum Inferred from direct assay PubMed 15872057. Source: RGD extracellular space Inferred from direct assay Ref.2. Source: RGD lysosome Inferred from direct assay PubMed 9222450. Source: RGD multivesicular body Inferred from direct assay PubMed 10950881. Source: RGD neuronal cell body Inferred from direct assay PubMed 14738488. Source: RGD nuclear membrane Inferred from direct assay PubMed 15872057. Source: RGD perinuclear region of cytoplasm Inferred from direct assay PubMed 16414118. Source: RGD vesicle Inferred from direct assay PubMed 10950881. Source: RGD
Molecular_function	beta-amyloid binding Inferred from electronic annotation. Source: Compara cysteine-type endopeptidase inhibitor activity Traceable author statement PubMed 16140167Ref.2. Source: RGD protease binding Inferred from direct assay PubMed 2622871PubMed 8761177. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 140	120	Cystatin-C		PRO_0000006644
Regions
	Motif	75 – 79	5	Secondary area of contact
Sites
	Site	31	1	Reactive site
Amino acid modifications
	Disulfide bond	93 ↔ 103		By similarity
	Disulfide bond	117 ↔ 137		By similarity
Experimental info
	Sequence conflict	38	1	A → E AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P14841 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: B785B2958F7824F8	FASTA	140	15,437
	10 20 30 40 50 60 MASPLRSLML LLAVLAVAWA GTSRPPPRLL GAPQEADASE EGVQRALDFA VSEYNKGSND 70 80 90 100 110 120 AYHSRAIQVV RARKQLVAGI NYYLDVEMGR TTCTKSQTNL TNCPFHDQPH LMRKALCSFQ 130 140 IYSVPWKGTH TLTKSSCKNA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[2]	"The cDNA structure and expression analysis of the genes for the cysteine proteinase inhibitor cystatin C and for beta 2-microglobulin in rat brain." Cole T., Dickson P.W., Esnard F., Averill F., Risbridger G., Gauthier F., Schreiber G. Eur. J. Biochem. 186:35-42(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-140. Strain: Buffalo.
[3]	"Rat cystatin C: the complete amino acid sequence reveals a site for N-glycosylation." Esnard F., Esnard A., Faucher D., Capony J.-P., Derancourt J., Brillard M., Gauthier F. Biol. Chem. Hoppe-Seyler 371:161-166(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-140.
[4]	"Two rat homologues of human cystatin C." Esnard A., Esnard F., Faucher D., Gauthier F. FEBS Lett. 236:475-478(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-62.
[5]	"Production of the cysteine proteinase inhibitor cystatin C by rat Sertoli cells." Esnard A., Esnard F., Guillou F., Gauthier F. FEBS Lett. 300:131-135(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 42-54. Tissue: Sertoli cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC087591 mRNA. Translation: AAH87591.1. X16957 mRNA. Translation: CAA34831.1.
IPI	IPI00231801.
PIR	S07085. S10587.
RefSeq	NP_036969.1. NM_012837.1.
UniGene	Rn.106351.
3D structure databases
ProteinModelPortal	P14841.
SMR	P14841. Positions 27-140.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000007175.
Protein family/group databases
MEROPS	I25.004.
PTM databases
PhosphoSite	P14841.
Proteomic databases
PaxDb	P14841.
PRIDE	P14841.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000007175; ENSRNOP00000007175; ENSRNOG00000005195.
GeneID	25307.
KEGG	rno:25307.
UCSC	RGD:2432. rat.
Organism-specific databases
CTD	1471.
RGD	2432. Cst3.
Phylogenomic databases
eggNOG	NOG44865.
GeneTree	ENSGT00570000079039.
HOGENOM	HOG000231754.
HOVERGEN	HBG009556.
InParanoid	P14841.
KO	K13899.
OMA	YTVPWLG.
OrthoDB	EOG49GKJ0.
Gene expression databases
Genevestigator	P14841.
GermOnline	ENSRNOG00000005195. Rattus norvegicus.
Family and domain databases
InterPro	IPR027214. Cystatin. IPR000010. Prot_inh_cystat. IPR018073. Prot_inh_cystat_CS. [Graphical view]
PANTHER	PTHR11413. PTHR11413. 1 hit.
Pfam	PF00031. Cystatin. 1 hit. [Graphical view]
SMART	SM00043. CY. 1 hit. [Graphical view]
PROSITE	PS00287. CYSTATIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	606107.

Entry information

Entry name

CYTC_RAT

Accession

Primary (citable) accession number: P14841
Secondary accession number(s): Q5M968

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	December 6, 2005
Last modified:	April 3, 2013
This is version 127 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program