Reviewed, UniProtKB/Swiss-Prot P14829 (GYRA_KLEOX)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3

Gene names

Name:

gyrA

Organism

Klebsiella oxytoca

Taxonomic identifier

571 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	876 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 876	876	DNA gyrase subunit A		PRO_0000145238
Sites
	Active site	122	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14829-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 7722CBFB07418D42	FASTA	876	97,155
	10 20 30 40 50 60 MSDLAREITP VNIEEELKSS YLDYAMSVIV GRALPDVRDG LKPVHRRVLY AMNVLGNDWN 70 80 90 100 110 120 KAYKKSARVV GDVIGKYHPH GDTAVYDTIV RMAQPFSLRY MLVDGQGNFG SVDGDSAAAM 130 140 150 160 170 180 RYTEIRMSKI AHELMADLEK ETVDFVDNYD GTEKIPDVMP TKIPNLLVNG SFGIAVGMAT 190 200 210 220 230 240 NIPPHNLTEV INGRLAYVED EEISIEGLME HIPGPDFPTA AIINGRRGIE EAYRTGRGKV 250 260 270 280 290 300 YICARAEVEA DAKTGRETII VHEIPYQVNK ARLIEKIAEL VKEKRVEGIS ALRDESDKDG 310 320 330 340 350 360 MRIVIEVKRD AVGRVVLNNL YSQTQLQVSF GINMVALHHG QPKIMNLKEI IAAFVRHRRE 370 380 390 400 410 420 VVTRRTILAL RKARDRADIL EALSIALANI DPIIELIRRA PTPAEAKAGL IARSWDLGNV 430 440 450 460 470 480 SAMLEAGDDA ARPEWLEPEF GVRDGQYYLT EQQAQAILDL RLQKLTGLEH EKLLDEYKEL 490 500 510 520 530 540 LEQIAELLHI LGSADRLMEV IREELELVRE QFGDARRTDI TANSVDINIE DLITQEDVVV 550 560 570 580 590 600 TLSHEGYVKY QPVNDYEAQR RGGKGKSAPR IKEEDFIDRL LVANTHDTIL CFSSRGRLYW 610 620 630 640 650 660 MKVYQVPEAS RGARGRPIVN LLPLEANERY TAILPVREYE EGVNVFMATA SGTVKKTPAD 670 680 690 700 710 720 EFSRPRSAGI IAVNLNEGDE LIGVDLTSGQ DEVMLFSAAG KVVRFKEDDV RAMGRTATGV 730 740 750 760 770 780 RGIKLAGEDK VVSLIVPRGE GRILTATENG YRKRTAVAEY PTKSRATQGV ISIKVTERNG 790 800 810 820 830 840 SVVGAVQVDD CDQIMMITDA GTLVRIRVSE VSIVGRNTQG VILIRTAEDE NVVALQRVAE 850 860 870 PVDDEELDAI DGSAAEGDED IAPEADTDDD IAEDEE

References

[1]	"Cloning and sequence analysis of gyrA gene of Klebsiella pneumoniae." Dimri G.P., Das H.K. Nucleic Acids Res. 18:151-156(1990) [PubMed: 2155395] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M5a1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X16817 Genomic DNA. Translation: CAA34724.1.
PIR	ITKBAP. S07722.
3D structure databases
HSSP	HSSP built from PDB template 1AB4 based on UniProtKB P09097.
SMR	P14829. Positions 30-521, 534-840.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.99.1.3. 81705.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_KLEOX

Accession

Primary (citable) accession number: P14829

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)